Rh7AG306900

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0004333
Physical Location & Seq
Forward (+)
34318994 .. 34322000
3007 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG306900.1

Sequence Viewer

Length: 420 bp
ATGGGAGGCGCTTCCATCACAACCGAACCTAACTCTTCCAACTCCACACTTCCGCTGCCTTCTCAGAAAAATGATGGCCAATCAGAGAATGGCAAAGAAGAAGAAGAAGAAGAAGATGCAGAGGAAGAGAAAGGTGAGTGTGGGTTTTGCTTATTCATGAAAGCAGGTCCGTGTGGAGAGAGATTCACAGCTTGGGAACAGTGCGTTAAAGAGTCTGAGAAGAACAAGGATTATGCTGCTGCAAAGTGTGTTGAGGTCTTCAAAACTTTGGATGAGTGTATGAAAGAGAATCAAGATTACTATGATTTTTTCTTGAGGGCCGAGAAAGCAGAATTGGAGAAGGAAGAGGATTTGATGAGAGAGAGCTCAGAGCACAAAGCCAGTTCAATTGGGGATTCTACTGACAAGCAAGAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

139

Amino Acids

15.6

Weight (kDa)

4.36

Isoelectric Point (pI)

71.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 45 - 114 6.6e-25 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 155
AciI CCGC 1 cut(s) 53
AcoI YGGCCR 1 cut(s) 76
AgsI TTSAA 2 cut(s) 262, 387
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 2 cut(s) 191, 366
AluI AGCT 2 cut(s) 191, 366
Alw21I GWGCWC 2 cut(s) 368, 375
AoxI GGCC 2 cut(s) 76, 318
ApeKI GCWGC 3 cut(s) 55, 236, 239
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 2 cut(s) 167, 318
AsuHPI GGTGA 1 cut(s) 146
AvaII GGWCC 1 cut(s) 167
BalI TGGCCA 1 cut(s) 78
BanII GRGCYC 1 cut(s) 368
BbsI GAAGAC 1 cut(s) 250
Bbv12I GWGCWC 2 cut(s) 368, 375
BbvI GCAGC 3 cut(s) 42, 223, 226
BccI CCATC 2 cut(s) 23, 68
BfoI RGCGCY 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 155
BisI GCNGC 3 cut(s) 56, 237, 240
BlsI GCNGC 3 cut(s) 57, 238, 241
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 167, 318
BmsI GCATC 1 cut(s) 106
BpiI GAAGAC 1 cut(s) 250
BpuEI CTTGAG 1 cut(s) 334
Bse1I ACTGG 1 cut(s) 381
BseGI GGATG 1 cut(s) 277
BseMII CTCAG 3 cut(s) 77, 207, 381
BseNI ACTGG 1 cut(s) 381
BseXI GCAGC 3 cut(s) 42, 223, 226
BshFI GGCC 2 cut(s) 78, 320
BsiHKAI GWGCWC 2 cut(s) 368, 375
BsnI GGCC 2 cut(s) 78, 320
Bsp1286I GDGCHC 2 cut(s) 368, 375
BspACI CCGC 1 cut(s) 53
BspANI GGCC 2 cut(s) 78, 320
BspCNI CTCAG 3 cut(s) 76, 208, 380
BspHI TCATGA 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 155
BsrI ACTGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 201
Bst6I CTCTTC 3 cut(s) 40, 120, 339
BstDEI CTNAG 3 cut(s) 63, 216, 367
BstF5I GGATG 1 cut(s) 277
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 326
BstV1I GCAGC 3 cut(s) 42, 223, 226
BstV2I GAAGAC 1 cut(s) 250
BsuRI GGCC 2 cut(s) 78, 320
BtsCI GGATG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 206
BveI ACCTGC 1 cut(s) 155
CciI TCATGA 1 cut(s) 156
CfoI GCGC 1 cut(s) 11
Cfr13I GGNCC 2 cut(s) 167, 318
CviAII CATG 1 cut(s) 157
CviJI RGCY 5 cut(s) 78, 191, 320, 366, 380
CviKI_1 RGCY 5 cut(s) 78, 191, 320, 366, 380
DdeI CTNAG 3 cut(s) 63, 216, 367
EaeI YGGCCR 1 cut(s) 76
Eam1104I CTCTTC 3 cut(s) 40, 120, 339
EarI CTCTTC 3 cut(s) 40, 120, 339
Ecl136II GAGCTC 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 167
Eco53kI GAGCTC 1 cut(s) 366
EcoICRI GAGCTC 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 368
FaeI CATG 1 cut(s) 160
FaiI YATR 4 cut(s) 158, 234, 281, 303
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 3 cut(s) 56, 237, 240
FokI GGATG 1 cut(s) 284
FriOI GRGCYC 1 cut(s) 368
Fsp4HI GCNGC 3 cut(s) 56, 237, 240
GlaI GCGC 1 cut(s) 10
GluI GCNGC 3 cut(s) 56, 237, 240
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 78, 320
HhaI GCGC 1 cut(s) 11
Hin1II CATG 1 cut(s) 160
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HinfI GANTC 4 cut(s) 183, 212, 289, 395
HphI GGTGA 1 cut(s) 146
Hpy188I TCNGA 4 cut(s) 66, 85, 217, 370
Hpy188III TCNNGA 3 cut(s) 157, 293, 313
HpyAV CCTTC 3 cut(s) 69, 334, 407
HpyCH4III ACNGT 1 cut(s) 201
HpyCH4V TGCA 2 cut(s) 119, 242
HpyF10VI GCNNNNNNNGC 1 cut(s) 326
HpyF3I CTNAG 3 cut(s) 63, 216, 367
Hsp92II CATG 1 cut(s) 160
HspAI GCGC 1 cut(s) 9
LpnPI CCDG 2 cut(s) 150, 394
Lsp1109I GCAGC 3 cut(s) 42, 223, 226
LweI GCATC 1 cut(s) 106
MfeI CAATTG 1 cut(s) 387
MhlI GDGCHC 2 cut(s) 368, 375
MlsI TGGCCA 1 cut(s) 78
MluCI AATT 2 cut(s) 332, 387
MluNI TGGCCA 1 cut(s) 78
MlyI GAGTC 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 63
MnlI CCTC 4 cut(s) 115, 247, 309, 340
Mox20I TGGCCA 1 cut(s) 78
MscI TGGCCA 1 cut(s) 78
MseI TTAA 1 cut(s) 207
Msp20I TGGCCA 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 55
MunI CAATTG 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 326
NlaIII CATG 1 cut(s) 160
NmeAIII GCCGAG 1 cut(s) 346
PagI TCATGA 1 cut(s) 156
PfeI GAWTC 3 cut(s) 183, 289, 395
PkrI GCNGC 3 cut(s) 57, 238, 241
PleI GAGTC 1 cut(s) 220
PpsI GAGTC 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 368
PspPI GGNCC 2 cut(s) 167, 318
SacI GAGCTC 1 cut(s) 368
SaqAI TTAA 1 cut(s) 207
SatI GCNGC 3 cut(s) 56, 237, 240
Sau96I GGNCC 2 cut(s) 167, 318
SchI GAGTC 1 cut(s) 221
SduI GDGCHC 2 cut(s) 368, 375
SetI ASST 7 cut(s) 31, 136, 169, 193, 258, 368, 418
SfaNI GCATC 1 cut(s) 106
SgeI CNNG 9 cut(s) 169, 177, 183, 204, 238, 305, 325, 334, 393
SinI GGWCC 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 313
SmoI CTYRAG 1 cut(s) 313
Sse9I AATT 2 cut(s) 332, 387
SsiI CCGC 1 cut(s) 53
SstI GAGCTC 1 cut(s) 368
TaaI ACNGT 1 cut(s) 201
TasI AATT 2 cut(s) 332, 387
TfiI GAWTC 3 cut(s) 183, 289, 395
Tru1I TTAA 1 cut(s) 207
Tru9I TTAA 1 cut(s) 207
TscAI CASTG 1 cut(s) 206
TseI GCWGC 3 cut(s) 55, 236, 239
TspDTI ATGAA 3 cut(s) 145, 173, 296
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 167
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.