RLG00000029137

GCK

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0004333
Physical Location & Seq
Reverse (-)
33581221 .. 33581817
597 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029137

Sequence Viewer

Length: 597 bp
ATGGGTGCTTCCAACTCCATTGACAGACCAGCACCAGACTCATCAGAGGCGTCTCCCCCACCAAAGACCGATTCAATTCCCTCCTCCGTCTCCGTCTCCATGTCCGATTCGGCTTCCAAACCCCAATCGGAAGACCAACCCCCGCCGCAGCCCCAATCGGACACAAAACCCTCCGAAGAAGAATCGGCGGCGGCGAATCCCAAGGAAGATGAGGCAGTCCAATCGGAGGAGGAGGAAGATGAGGAGGAGAAGGGGGAGTGTGGGTTCTGCTTGTACATGAAAGGCGGCGGGTGCAAAGAGAGCTTTATAGCTTGGGAAAACTGCATTGAAGAGGCGGAGAAGACGAACGAGGACATTACTCAGAAGTGCTTCGATCTCACCTCCGCTCTCAGGACATGTATGCTGGAACATTCCGATTACTACGAGCCCATTCTCAGGGCGGAGAAGGCCGCCGAGGAAGAAGCCATCAAGGAACTCGACAAGGAAAAGAAGGAAGAGAAGGAGAAGGAGAAGGAGAAGGAGGTGGTTATAGAATTGGAGAAAGAAAAGGCTCCAGCTTCTTCGGAGGAGGAGGAGAGTTCCGGTGGGAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GCK

Protein Analysis

199

Amino Acids

21.83

Weight (kDa)

4.3

Isoelectric Point (pI)

90.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCK PF07802 85 - 158 6.3e-35 GCK domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 386
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 3 cut(s) 226, 390, 435
AflIII ACRYGT 1 cut(s) 395
AgsI TTSAA 2 cut(s) 75, 329
AjuI GAANNNNNNNTTGG 2 cut(s) 55, 87
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 3 cut(s) 303, 311, 557
AluI AGCT 3 cut(s) 303, 311, 557
Alw26I GTCTC 3 cut(s) 57, 94, 100
AoxI GGCC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 148
Asp700I GAANNNNTTC 1 cut(s) 368
AsuHPI GGTGA 1 cut(s) 370
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 2 cut(s) 138, 347
BbvI GCAGC 1 cut(s) 160
BccI CCATC 1 cut(s) 473
BcgI CGANNNNNNTGC 2 cut(s) 204, 238
BcoDI GTCTC 3 cut(s) 57, 94, 100
BisI GCNGC 6 cut(s) 146, 149, 189, 192, 286, 450
BlsI GCNGC 6 cut(s) 147, 150, 190, 193, 287, 451
BmiI GGNNCC 1 cut(s) 552
BpiI GAAGAC 2 cut(s) 138, 347
BpmI CTGGAG 1 cut(s) 537
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 201, 453
BsaWI WCCGGW 1 cut(s) 581
BsaXI ACNNNNNCTCC 2 cut(s) 248, 278
Bsc4I CCNNNNNNNGG 3 cut(s) 226, 390, 435
BseDI CCNNGG 2 cut(s) 201, 453
BseLI CCNNNNNNNGG 3 cut(s) 226, 390, 435
BseMII CTCAG 3 cut(s) 374, 403, 448
BseRI GAGGAG 8 cut(s) 73, 242, 245, 257, 260, 581, 584, 587
BseXI GCAGC 1 cut(s) 160
BshFI GGCC 1 cut(s) 449
BsiSI CCGG 1 cut(s) 582
BslI CCNNNNNNNGG 3 cut(s) 226, 390, 435
BsmAI GTCTC 3 cut(s) 57, 94, 100
BsmBI CGTCTC 3 cut(s) 57, 94, 100
BsnI GGCC 1 cut(s) 449
Bsp1286I GDGCHC 1 cut(s) 429
Bsp1407I TGTACA 1 cut(s) 273
Bsp143I GATC 1 cut(s) 373
BspANI GGCC 1 cut(s) 449
BspCNI CTCAG 3 cut(s) 373, 402, 447
BspLI GGNNCC 1 cut(s) 552
BsrBI CCGCTC 1 cut(s) 386
BsrGI TGTACA 1 cut(s) 273
BssECI CCNNGG 2 cut(s) 201, 453
BssMI GATC 1 cut(s) 373
BssNI GRCGYC 1 cut(s) 50
BssT1I CCWWGG 1 cut(s) 201
Bst6I CTCTTC 2 cut(s) 324, 489
BstACI GRCGYC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 273
BstDEI CTNAG 3 cut(s) 360, 389, 434
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 3 cut(s) 57, 94, 100
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 3 cut(s) 291, 300, 446
BstNSI RCATGY 1 cut(s) 399
BstV1I GCAGC 1 cut(s) 160
BstV2I GAAGAC 2 cut(s) 138, 347
BsuRI GGCC 1 cut(s) 449
CseI GACGC 1 cut(s) 39
Csp6I GTAC 1 cut(s) 274
CviAII CATG 3 cut(s) 100, 277, 396
CviJI RGCY 9 cut(s) 113, 151, 303, 311, 427, 449, 464, 551, 557
CviKI_1 RGCY 9 cut(s) 113, 151, 303, 311, 427, 449, 464, 551, 557
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 3 cut(s) 360, 389, 434
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
Eam1104I CTCTTC 2 cut(s) 324, 489
EarI CTCTTC 2 cut(s) 324, 489
EciI GGCGGA 2 cut(s) 350, 455
Eco130I CCWWGG 1 cut(s) 201
Eco24I GRGCYC 1 cut(s) 429
EcoT14I CCWWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 429
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 3 cut(s) 57, 94, 100
FaeI CATG 3 cut(s) 103, 280, 399
FaiI YATR 6 cut(s) 101, 278, 308, 397, 401, 530
FatI CATG 3 cut(s) 99, 276, 395
FauI CCCGC 2 cut(s) 150, 281
Fnu4HI GCNGC 6 cut(s) 146, 149, 189, 192, 286, 450
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 6 cut(s) 146, 149, 189, 192, 286, 450
GluI GCNGC 6 cut(s) 146, 149, 189, 192, 286, 450
GsuI CTGGAG 1 cut(s) 537
HaeIII GGCC 1 cut(s) 449
HapII CCGG 1 cut(s) 582
HgaI GACGC 1 cut(s) 39
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 3 cut(s) 103, 280, 399
HinfI GANTC 5 cut(s) 38, 71, 107, 182, 196
HpaII CCGG 1 cut(s) 582
HphI GGTGA 1 cut(s) 370
Hpy188I TCNGA 9 cut(s) 46, 106, 130, 160, 175, 226, 363, 415, 565
Hpy188III TCNNGA 1 cut(s) 391
HpyAV CCTTC 7 cut(s) 244, 439, 484, 493, 499, 505, 511
HpyCH4V TGCA 2 cut(s) 294, 324
HpyF10VI GCNNNNNNNGC 3 cut(s) 291, 300, 446
HpyF3I CTNAG 3 cut(s) 360, 389, 434
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 3 cut(s) 103, 280, 399
Kzo9I GATC 1 cut(s) 373
LmnI GCTCC 1 cut(s) 556
LpnPI CCDG 6 cut(s) 42, 48, 376, 389, 421, 567
Lsp1109I GCAGC 1 cut(s) 160
MalI GATC 1 cut(s) 375
MbiI CCGCTC 1 cut(s) 386
MboI GATC 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 2 cut(s) 75, 533
MlyI GAGTC 1 cut(s) 32
MmeI TCCRAC 1 cut(s) 36
MroXI GAANNNNTTC 1 cut(s) 368
MspI CCGG 1 cut(s) 582
MwoI GCNNNNNNNGC 3 cut(s) 291, 300, 446
NdeII GATC 1 cut(s) 373
NlaIII CATG 3 cut(s) 103, 280, 399
NlaIV GGNNCC 1 cut(s) 552
NmeAIII GCCGAG 1 cut(s) 478
NspI RCATGY 1 cut(s) 399
PciI ACATGT 1 cut(s) 395
PcsI WCGNNNNNNNCGW 1 cut(s) 191
PdmI GAANNNNTTC 1 cut(s) 368
PfeI GAWTC 4 cut(s) 71, 107, 182, 196
PkrI GCNGC 6 cut(s) 147, 150, 190, 193, 287, 451
PleI GAGTC 1 cut(s) 32
PpsI GAGTC 1 cut(s) 32
PscI ACATGT 1 cut(s) 395
PspN4I GGNNCC 1 cut(s) 552
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
SatI GCNGC 6 cut(s) 146, 149, 189, 192, 286, 450
Sau3AI GATC 1 cut(s) 373
SchI GAGTC 1 cut(s) 32
SduI GDGCHC 1 cut(s) 429
SetI ASST 5 cut(s) 305, 313, 383, 525, 559
Sse9I AATT 2 cut(s) 75, 533
StyI CCWWGG 1 cut(s) 201
TaqI TCGA 2 cut(s) 372, 477
TaqII GACCGA 1 cut(s) 83
TasI AATT 2 cut(s) 75, 533
TatI WGTACW 1 cut(s) 273
TauI GCSGC 5 cut(s) 148, 191, 194, 288, 452
TfiI GAWTC 4 cut(s) 71, 107, 182, 196
TseI GCWGC 1 cut(s) 148
TspDTI ATGAA 1 cut(s) 293
TspGWI ACGGA 2 cut(s) 76, 82
XceI RCATGY 1 cut(s) 399
XmnI GAANNNNTTC 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.