AT1G19400

At1g01500-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001242
Physical Location & Seq
Reverse (-)
6712022 .. 6714483
2462 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G19400.2

Sequence Viewer

Length: 912 bp
ATGGAGGAGCCTTGTGAGACACTTGACAGTGGTGAAGCCTATCAAATATCTAGATATCATAACTATAACCAACACAGCTCCAGAAGCTCATCTCCCTGGTTGGATTTGAGAGTGTTCTATGTCCGGATAAGCAATTTCGTGGTGGAAGATTCAACTCCTGAGGTCCTCACTATCAATCACATTCCTCTGGATCCAGATACGCTTTTGGAGATTAACGGTGTTAGAATGGGAATGTACTCAGAAGGAGGTTCTTCACAGCTTAGGAGAGATCGGGTTGATAAGAAATCTGAAGAAGCTACTTATGTCAGCACAGACAATATCAGGTTAACTGGTAGTGTGAAGTTTGAGGTTTATGACAAAAATGAGCTAGTTTTGTCTGGAACTCTTGAGATGTCTGGTAGTAATGGTTTCACTGGGGAATCAAAGAATCGATGGAAAATGAATTGTGAGGCTGAGGTCACTGCAGGGTCTGGTTTCCTTAAGGAGAAGAGCATAAACGGTCAAGAGTTATCGTCTCCATTGCCAACTATCGAGGTCTATGTTACTGGTTGCTTCTCAGGAACACCCATCATCTTAACCAAGACTCTGCAACTTGGTTTGAAAAAGAAGCAGAGTAGAAGAATGGCATTGGATGCAATTCCTGAGTATGAAACTGCAGAGCCTCAGAAAGACACCTCCTATGCACTTGATCTTCAGGCAACAACAGAGTACGGGAATTACAAAGAGGAATACGAAGGAGACATGTATTGGAGAAGTGAGTGCATTGATGGGGAGATGTCATGGTTTAATGCAGGTGTAAGGGTTGGTGTTGGGATAGGACTTGGTGTCTGTGTTGGTCTTGGGATTGGTGTCGGTCTTCTCGTTCGTACTTATCAATCAACTACCAGAACCTTCAGGAGGAGGCTTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

303

Amino Acids

33.95

Weight (kDa)

5.09

Isoelectric Point (pI)

55.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 782
Acc36I ACCTGC 1 cut(s) 782
AccIII TCCGGA 1 cut(s) 123
AclWI GGATC 2 cut(s) 185, 198
AcuI CTGAAG 3 cut(s) 309, 677, 877
AfaI GTAC 3 cut(s) 236, 710, 868
AfiI CCNNNNNNNGG 1 cut(s) 897
AflII CTTAAG 1 cut(s) 479
AflIII ACRYGT 1 cut(s) 741
AgsI TTSAA 2 cut(s) 153, 601
AhdI GACNNNNNGTC 1 cut(s) 824
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 5 cut(s) 78, 87, 259, 296, 367
AluI AGCT 5 cut(s) 78, 87, 259, 296, 367
Alw26I GTCTC 3 cut(s) 11, 519, 732
AlwI GGATC 2 cut(s) 185, 198
AlwNI CAGNNNCTG 1 cut(s) 470
Aor13HI TCCGGA 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 163
AxyI CCTNAGG 1 cut(s) 159
BamHI GGATCC 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 848
BbvCI CCTCAGC 1 cut(s) 453
BccI CCATC 3 cut(s) 426, 575, 761
BciT130I CCWGG 1 cut(s) 97
BcoDI GTCTC 3 cut(s) 11, 519, 732
BfaI CTAG 3 cut(s) 51, 368, 910
BfmI CTRYAG 2 cut(s) 462, 654
BfrI CTTAAG 1 cut(s) 479
BfuAI ACCTGC 1 cut(s) 782
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 163
BmeRI GACNNNNNGTC 1 cut(s) 824
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 2 cut(s) 9, 192
BmrFI CCNGG 1 cut(s) 97
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 1 cut(s) 622
BmuI ACTGGG 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 848
BpmI CTGGAG 1 cut(s) 64
Bpu10I CCTNAGC 2 cut(s) 260, 453
BpuEI CTTGAG 1 cut(s) 407
Bsa29I ATCGAT 1 cut(s) 430
BsaJI CCNNGG 1 cut(s) 95
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 1 cut(s) 897
Bse1I ACTGG 3 cut(s) 334, 418, 550
Bse21I CCTNAGG 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 518
BseAI TCCGGA 1 cut(s) 123
BseBI CCWGG 1 cut(s) 97
BseCI ATCGAT 1 cut(s) 430
BseDI CCNNGG 1 cut(s) 95
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 1 cut(s) 897
BseMI GCAATG 1 cut(s) 518
BseMII CTCAG 6 cut(s) 150, 252, 444, 570, 633, 677
BseNI ACTGG 3 cut(s) 334, 418, 550
BseRI GAGGAG 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 430
BsiSI CCGG 1 cut(s) 124
BslI CCNNNNNNNGG 1 cut(s) 897
BsmAI GTCTC 3 cut(s) 11, 519, 732
BsmBI CGTCTC 1 cut(s) 519
Bsp13I TCCGGA 1 cut(s) 123
Bsp143I GATC 3 cut(s) 190, 268, 688
BspCNI CTCAG 6 cut(s) 151, 251, 445, 569, 634, 676
BspDI ATCGAT 1 cut(s) 430
BspEI TCCGGA 1 cut(s) 123
BspLI GGNNCC 2 cut(s) 9, 192
BspMAI CTGCAG 2 cut(s) 466, 658
BspMI ACCTGC 1 cut(s) 782
BspPI GGATC 2 cut(s) 185, 198
BspQI GCTCTTC 1 cut(s) 482
BspTI CTTAAG 1 cut(s) 479
BsrDI GCAATG 1 cut(s) 518
BsrI ACTGG 3 cut(s) 334, 418, 550
BssECI CCNNGG 1 cut(s) 95
BssMI GATC 3 cut(s) 190, 268, 688
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 29, 218, 500
Bst6I CTCTTC 1 cut(s) 482
BstAFI CTTAAG 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 632
BstDEI CTNAG 7 cut(s) 159, 238, 260, 453, 556, 642, 663
BstENI CCTNNNNNAGG 1 cut(s) 895
BstF5I GGATG 1 cut(s) 637
BstKTI GATC 3 cut(s) 193, 271, 691
BstMAI GTCTC 3 cut(s) 11, 519, 732
BstMBI GATC 3 cut(s) 190, 268, 688
BstMWI GCNNNNNNNGC 2 cut(s) 84, 632
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 1 cut(s) 745
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 2 cut(s) 462, 654
BstV2I GAAGAC 1 cut(s) 848
BstX2I RGATCY 1 cut(s) 190
BstYI RGATCY 1 cut(s) 190
Bsu15I ATCGAT 1 cut(s) 430
Bsu36I CCTNAGG 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 430
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 3 cut(s) 34, 411, 459
BveI ACCTGC 1 cut(s) 782
CaiI CAGNNNCTG 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 163
ClaI ATCGAT 1 cut(s) 430
Csp6I GTAC 3 cut(s) 235, 709, 867
CviAII CATG 2 cut(s) 742, 780
CviQI GTAC 3 cut(s) 235, 709, 867
DdeI CTNAG 7 cut(s) 159, 238, 260, 453, 556, 642, 663
DpnI GATC 3 cut(s) 192, 270, 690
DpnII GATC 3 cut(s) 190, 268, 688
DriI GACNNNNNGTC 1 cut(s) 824
Eam1104I CTCTTC 1 cut(s) 482
Eam1105I GACNNNNNGTC 1 cut(s) 824
EarI CTCTTC 1 cut(s) 482
Eco32I GATATC 1 cut(s) 56
Eco47I GGWCC 1 cut(s) 163
Eco57I CTGAAG 3 cut(s) 309, 677, 877
Eco81I CCTNAGG 1 cut(s) 159
EcoNI CCTNNNNNAGG 1 cut(s) 895
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 95
EcoRV GATATC 1 cut(s) 56
Esp3I CGTCTC 1 cut(s) 519
FaeI CATG 2 cut(s) 745, 783
FatI CATG 2 cut(s) 741, 779
FokI GGATG 1 cut(s) 644
FspBI CTAG 3 cut(s) 51, 368, 910
GsuI CTGGAG 1 cut(s) 64
HapII CCGG 1 cut(s) 124
Hin1II CATG 2 cut(s) 745, 783
HincII GTYRAC 1 cut(s) 327
HindII GTYRAC 1 cut(s) 327
HinfI GANTC 4 cut(s) 149, 419, 427, 583
HpaI GTTAAC 1 cut(s) 327
HpaII CCGG 1 cut(s) 124
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 327
Hpy188I TCNGA 3 cut(s) 241, 289, 666
Hpy8I GTNNAC 1 cut(s) 327
HpyAV CCTTC 3 cut(s) 236, 728, 901
HpyCH4III ACNGT 3 cut(s) 29, 218, 500
HpyCH4V TGCA 7 cut(s) 464, 589, 635, 656, 683, 762, 791
HpyF10VI GCNNNNNNNGC 2 cut(s) 84, 632
HpyF3I CTNAG 7 cut(s) 159, 238, 260, 453, 556, 642, 663
Hsp92II CATG 2 cut(s) 745, 783
Kpn2I TCCGGA 1 cut(s) 123
KspAI GTTAAC 1 cut(s) 327
Kzo9I GATC 3 cut(s) 190, 268, 688
LguI GCTCTTC 1 cut(s) 482
LmnI GCTCC 2 cut(s) 7, 83
LweI GCATC 1 cut(s) 622
MaeI CTAG 3 cut(s) 51, 368, 910
MaeIII GTNAC 2 cut(s) 457, 541
MalI GATC 3 cut(s) 192, 270, 690
MboI GATC 3 cut(s) 190, 268, 688
MboII GAAGA 7 cut(s) 158, 243, 302, 499, 630, 683, 848
MflI RGATCY 1 cut(s) 190
MluCI AATT 4 cut(s) 133, 442, 636, 715
MlyI GAGTC 1 cut(s) 577
MmeI TCCRAC 1 cut(s) 81
MroI TCCGGA 1 cut(s) 123
MseI TTAA 5 cut(s) 213, 326, 480, 575, 786
MspCI CTTAAG 1 cut(s) 479
MspI CCGG 1 cut(s) 124
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 84, 632
NdeII GATC 3 cut(s) 190, 268, 688
NlaIII CATG 2 cut(s) 745, 783
NlaIV GGNNCC 2 cut(s) 9, 192
NmuCI GTSAC 1 cut(s) 457
NspI RCATGY 1 cut(s) 745
PaqCI CACCTGC 1 cut(s) 782
PciI ACATGT 1 cut(s) 741
PciSI GCTCTTC 1 cut(s) 482
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PfeI GAWTC 3 cut(s) 149, 419, 427
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
PpuMI RGGWCCY 1 cut(s) 163
PscI ACATGT 1 cut(s) 741
Psp5II RGGWCCY 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 2 cut(s) 9, 192
PspPI GGNCC 1 cut(s) 163
PspPPI RGGWCCY 1 cut(s) 163
PstI CTGCAG 2 cut(s) 466, 658
PstNI CAGNNNCTG 1 cut(s) 470
PsuI RGATCY 1 cut(s) 190
RsaI GTAC 3 cut(s) 236, 710, 868
RsaNI GTAC 3 cut(s) 235, 709, 867
SapI GCTCTTC 1 cut(s) 482
SaqAI TTAA 5 cut(s) 213, 326, 480, 575, 786
Sau3AI GATC 3 cut(s) 190, 268, 688
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 577
ScrFI CCNGG 1 cut(s) 97
SfaNI GCATC 1 cut(s) 622
SfcI CTRYAG 2 cut(s) 462, 654
SinI GGWCC 1 cut(s) 163
SmlI CTYRAG 2 cut(s) 386, 479
SmoI CTYRAG 2 cut(s) 386, 479
Sse9I AATT 4 cut(s) 133, 442, 636, 715
SspMI CTAG 3 cut(s) 51, 368, 910
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 3 cut(s) 29, 218, 500
TaqI TCGA 2 cut(s) 430, 531
TaqII GACCGA 1 cut(s) 842
TasI AATT 4 cut(s) 133, 442, 636, 715
TatI WGTACW 1 cut(s) 234
TfiI GAWTC 3 cut(s) 149, 419, 427
Tru1I TTAA 5 cut(s) 213, 326, 480, 575, 786
Tru9I TTAA 5 cut(s) 213, 326, 480, 575, 786
TscAI CASTG 3 cut(s) 34, 418, 466
TseFI GTSAC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 2 cut(s) 455, 663
TspRI CASTG 3 cut(s) 34, 418, 466
Vha464I CTTAAG 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 163
XagI CCTNNNNNAGG 1 cut(s) 895
XbaI TCTAGA 1 cut(s) 50
XceI RCATGY 1 cut(s) 745
XspI CTAG 3 cut(s) 51, 368, 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.