Rh6BG127600

At1g01500-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001242
Physical Location & Seq
Reverse (-)
19910163 .. 19912132
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG127600.1

Sequence Viewer

Length: 990 bp
ATGGAAAATTCTTATGAGACATCAAACAGCCACACACCTATTTTCAATGGCTATGCACCTACTTCAAATGGCTATGGACCTATTTCAAATGGTCGTGGGTCAACTGGTGATGGCCACATGATTGGGAGACAATCCACCTACCAACCCAAGGTGTCACTACCTTGGCTTGATGTAAGAGTTTTCTATGTTAGGATTAGCAAATGTGAGATTGATGATTCGGCTCCAGAATTCCTCACGGTAAATCACATTCCATTGGATGCTGATACCCTTCTTGAAGTCAATGGTGTTAGAACTAGCATGTATTCAGATGGGGAAACAACTCTTCTTCGAAGAGACCGGTTAGATAAGAAGTCAGAAGAAGCTACGTTTGTCAGCACTGATAGTATAAGGATGACTGGAAGTGTGAAGTTTGAGGTTTTTGACAAGGATGTTATCGTGCTATCTGGGGTTTTAGAGTTGTGTAATAATATTGGTTTCATTGCGGAAACAGATCATCATGGCCCGAGATGGAGCATGAATTGTGAAACAAATATAGTTGCAAGCACTGGCTTCTTTAAACCAAAGCAGTTTACGGGTTCAGAGTCTGCAACACCCTCAATTGAAGTATACATTGCAGGGTCCTTCTTGGGAACTCCAATAATTCTAACAAAGACTTTACAGCTGAGTCCTTGGAAGAAGCATACGAGGAAGGGGTTATTGGATTCAATACCAGAGTATGATGCAACTGAAGACGAGAACAATAATCCTTCTAGGTTCATGTTTCAGGGACCAGAATACTTGGACCTGAAACCAGAATATGGAGAAAATAACTTGTACTACTCTGGGACATATTTAGAAGGTGAAGATGGGGAGCTTTCATGGTTTAATGCTGGTGTGAGAGTTGGTGTTGGGATTGGCTTGAGCATATGCGTTGGGGTTGGCATCGGAGTAGGTTTGCTTGTTCGAACTTACCAAGGCACCACTCAGAGCTTTAGAAGGCGGCTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

329

Amino Acids

36.47

Weight (kDa)

5.1

Isoelectric Point (pI)

32.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 956
AccB7I CCANNNNNTGG 1 cut(s) 797
AccI GTMKAC 1 cut(s) 606
AciI CCGC 2 cut(s) 482, 979
AcoI YGGCCR 1 cut(s) 112
AcsI RAATTY 2 cut(s) 7, 227
AcuI CTGAAG 1 cut(s) 747
AfaI GTAC 1 cut(s) 815
AfiI CCNNNNNNNGG 2 cut(s) 148, 797
AgeI ACCGGT 1 cut(s) 336
AgsI TTSAA 6 cut(s) 46, 66, 87, 275, 602, 705
AjuI GAANNNNNNNTTGG 2 cut(s) 680, 712
AluBI AGCT 4 cut(s) 362, 661, 853, 969
AluI AGCT 4 cut(s) 362, 661, 853, 969
Alw26I GTCTC 3 cut(s) 11, 121, 327
AlwNI CAGNNNCTG 1 cut(s) 584
Ama87I CYCGRG 1 cut(s) 502
AoxI GGCC 2 cut(s) 112, 499
ApoI RAATTY 2 cut(s) 7, 227
AsiGI ACCGGT 1 cut(s) 336
AspS9I GGNCC 5 cut(s) 77, 500, 618, 767, 781
AsuHPI GGTGA 2 cut(s) 119, 851
AsuII TTCGAA 2 cut(s) 328, 943
AvaI CYCGRG 1 cut(s) 502
AvaII GGWCC 4 cut(s) 77, 618, 767, 781
BalI TGGCCA 1 cut(s) 114
BanI GGYRCC 1 cut(s) 956
BbsI GAAGAC 1 cut(s) 735
BccI CCATC 4 cut(s) 104, 302, 501, 839
BcoDI GTCTC 3 cut(s) 11, 121, 327
BfaI CTAG 2 cut(s) 294, 750
BisI GCNGC 1 cut(s) 980
BlsI GCNGC 1 cut(s) 981
Bme18I GGWCC 4 cut(s) 77, 618, 767, 781
BmeT110I CYCGRG 1 cut(s) 502
BmgT120I GGNCC 5 cut(s) 77, 500, 618, 767, 781
BmiI GGNNCC 4 cut(s) 222, 619, 768, 958
BmsI GCATC 3 cut(s) 247, 709, 930
BpiI GAAGAC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 207
Bpu14I TTCGAA 2 cut(s) 328, 943
BpuEI CTTGAG 1 cut(s) 919
BsaI GGTCTC 1 cut(s) 327
BsaJI CCNNGG 4 cut(s) 147, 161, 668, 952
BsaWI WCCGGW 1 cut(s) 336
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 797
Bse118I RCCGGY 1 cut(s) 336
Bse1I ACTGG 3 cut(s) 109, 400, 550
Bse3DI GCAATG 2 cut(s) 477, 609
BseDI CCNNGG 4 cut(s) 147, 161, 668, 952
BseGI GGATG 3 cut(s) 262, 396, 433
BseLI CCNNNNNNNGG 2 cut(s) 148, 797
BseMI GCAATG 2 cut(s) 477, 609
BseMII CTCAG 2 cut(s) 653, 977
BseNI ACTGG 3 cut(s) 109, 400, 550
BshFI GGCC 2 cut(s) 114, 501
BshNI GGYRCC 1 cut(s) 956
BshTI ACCGGT 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 502
BsiSI CCGG 1 cut(s) 337
BslFI GGGAC 2 cut(s) 780, 838
BslI CCNNNNNNNGG 2 cut(s) 148, 797
BsmAI GTCTC 3 cut(s) 11, 121, 327
BsmFI GGGAC 2 cut(s) 780, 838
BsnI GGCC 2 cut(s) 114, 501
Bso31I GGTCTC 1 cut(s) 327
BsoBI CYCGRG 1 cut(s) 502
Bsp119I TTCGAA 2 cut(s) 328, 943
Bsp143I GATC 1 cut(s) 490
BspACI CCGC 2 cut(s) 482, 979
BspANI GGCC 2 cut(s) 114, 501
BspCNI CTCAG 2 cut(s) 654, 976
BspLI GGNNCC 4 cut(s) 222, 619, 768, 958
BspT104I TTCGAA 2 cut(s) 328, 943
BspT107I GGYRCC 1 cut(s) 956
BspTNI GGTCTC 1 cut(s) 327
BsrDI GCAATG 2 cut(s) 477, 609
BsrFI RCCGGY 1 cut(s) 336
BsrI ACTGG 3 cut(s) 109, 400, 550
BssAI RCCGGY 1 cut(s) 336
BssECI CCNNGG 4 cut(s) 147, 161, 668, 952
BssMI GATC 1 cut(s) 490
BssNAI GTATAC 1 cut(s) 607
BssT1I CCWWGG 4 cut(s) 147, 161, 668, 952
Bst1107I GTATAC 1 cut(s) 607
Bst4CI ACNGT 2 cut(s) 238, 987
Bst6I CTCTTC 2 cut(s) 325, 327
BstBI TTCGAA 2 cut(s) 328, 943
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 2 cut(s) 662, 963
BstF5I GGATG 3 cut(s) 262, 396, 433
BstKTI GATC 1 cut(s) 493
BstMAI GTCTC 3 cut(s) 11, 121, 327
BstMBI GATC 1 cut(s) 490
BstNSI RCATGY 1 cut(s) 301
BstV2I GAAGAC 1 cut(s) 735
BstXI CCANNNNNNTGG 1 cut(s) 122
BstZ17I GTATAC 1 cut(s) 607
BsuRI GGCC 2 cut(s) 114, 501
BtsCI GGATG 3 cut(s) 262, 396, 433
BtsIMutI CAGTG 2 cut(s) 375, 543
Cac8I GCNNGC 1 cut(s) 541
CaiI CAGNNNCTG 1 cut(s) 584
Cfr10I RCCGGY 1 cut(s) 336
Cfr13I GGNCC 5 cut(s) 77, 500, 618, 767, 781
Csp6I GTAC 1 cut(s) 814
CspAI ACCGGT 1 cut(s) 336
CviAII CATG 6 cut(s) 118, 298, 497, 514, 757, 858
CviQI GTAC 1 cut(s) 814
DdeI CTNAG 2 cut(s) 662, 963
DpnI GATC 1 cut(s) 492
DpnII GATC 1 cut(s) 490
DraI TTTAAA 1 cut(s) 556
EaeI YGGCCR 1 cut(s) 112
Eam1104I CTCTTC 2 cut(s) 325, 327
EarI CTCTTC 2 cut(s) 325, 327
Eco130I CCWWGG 4 cut(s) 147, 161, 668, 952
Eco31I GGTCTC 1 cut(s) 327
Eco47I GGWCC 4 cut(s) 77, 618, 767, 781
Eco57I CTGAAG 1 cut(s) 747
Eco88I CYCGRG 1 cut(s) 502
EcoO109I RGGNCCY 1 cut(s) 618
EcoRI GAATTC 1 cut(s) 227
EcoT14I CCWWGG 4 cut(s) 147, 161, 668, 952
ErhI CCWWGG 4 cut(s) 147, 161, 668, 952
FaeI CATG 6 cut(s) 121, 301, 500, 517, 760, 861
FaqI GGGAC 2 cut(s) 780, 838
FatI CATG 6 cut(s) 117, 297, 496, 513, 756, 857
FauNDI CATATG 1 cut(s) 905
FblI GTMKAC 1 cut(s) 606
Fnu4HI GCNGC 1 cut(s) 980
FokI GGATG 3 cut(s) 269, 403, 440
Fsp4HI GCNGC 1 cut(s) 980
FspBI CTAG 2 cut(s) 294, 750
GluI GCNGC 1 cut(s) 980
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 2 cut(s) 114, 501
HapII CCGG 1 cut(s) 337
Hin1II CATG 6 cut(s) 121, 301, 500, 517, 760, 861
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HinfI GANTC 4 cut(s) 215, 581, 664, 701
HpaII CCGG 1 cut(s) 337
HphI GGTGA 2 cut(s) 119, 851
Hpy166II GTNNAC 3 cut(s) 102, 570, 607
Hpy188I TCNGA 5 cut(s) 307, 355, 580, 926, 966
Hpy188III TCNNGA 2 cut(s) 224, 272
Hpy8I GTNNAC 3 cut(s) 102, 570, 607
HpyAV CCTTC 6 cut(s) 278, 631, 682, 756, 830, 969
HpyCH4III ACNGT 2 cut(s) 238, 987
HpyCH4IV ACGT 1 cut(s) 365
HpyCH4V TGCA 5 cut(s) 56, 539, 587, 614, 722
HpyF3I CTNAG 2 cut(s) 662, 963
HpySE526I ACGT 1 cut(s) 365
Hsp92II CATG 6 cut(s) 121, 301, 500, 517, 760, 861
Kzo9I GATC 1 cut(s) 490
LmnI GCTCC 3 cut(s) 226, 510, 850
LweI GCATC 3 cut(s) 247, 709, 930
MaeI CTAG 2 cut(s) 294, 750
MaeII ACGT 1 cut(s) 365
MaeIII GTNAC 1 cut(s) 153
MalI GATC 1 cut(s) 492
MboI GATC 1 cut(s) 490
MboII GAAGA 7 cut(s) 314, 317, 342, 368, 685, 740, 854
MfeI CAATTG 1 cut(s) 597
MlsI TGGCCA 1 cut(s) 114
MluCI AATT 5 cut(s) 7, 227, 517, 597, 639
MluNI TGGCCA 1 cut(s) 114
MlyI GAGTC 2 cut(s) 590, 673
MnlI CCTC 4 cut(s) 242, 406, 604, 678
Mox20I TGGCCA 1 cut(s) 114
MscI TGGCCA 1 cut(s) 114
MseI TTAA 2 cut(s) 555, 864
Msp20I TGGCCA 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 661
MspI CCGG 1 cut(s) 337
MunI CAATTG 1 cut(s) 597
NdeI CATATG 1 cut(s) 905
NdeII GATC 1 cut(s) 490
NlaIII CATG 6 cut(s) 121, 301, 500, 517, 760, 861
NlaIV GGNNCC 4 cut(s) 222, 619, 768, 958
NmuCI GTSAC 1 cut(s) 153
NspI RCATGY 1 cut(s) 301
NspV TTCGAA 2 cut(s) 328, 943
PfeI GAWTC 2 cut(s) 215, 701
PflMI CCANNNNNTGG 1 cut(s) 797
PinAI ACCGGT 1 cut(s) 336
PkrI GCNGC 1 cut(s) 981
PleI GAGTC 2 cut(s) 589, 672
PpsI GAGTC 2 cut(s) 589, 672
PpuMI RGGWCCY 1 cut(s) 618
Psp5II RGGWCCY 1 cut(s) 618
PspN4I GGNNCC 4 cut(s) 222, 619, 768, 958
PspPI GGNCC 5 cut(s) 77, 500, 618, 767, 781
PspPPI RGGWCCY 1 cut(s) 618
PstNI CAGNNNCTG 1 cut(s) 584
PvuII CAGCTG 1 cut(s) 661
RsaI GTAC 1 cut(s) 815
RsaNI GTAC 1 cut(s) 814
SaqAI TTAA 2 cut(s) 555, 864
SatI GCNGC 1 cut(s) 980
Sau3AI GATC 1 cut(s) 490
Sau96I GGNCC 5 cut(s) 77, 500, 618, 767, 781
SchI GAGTC 2 cut(s) 590, 673
SfaNI GCATC 3 cut(s) 247, 709, 930
SfuI TTCGAA 2 cut(s) 328, 943
SinI GGWCC 4 cut(s) 77, 618, 767, 781
SmlI CTYRAG 1 cut(s) 898
SmoI CTYRAG 1 cut(s) 898
Sse9I AATT 5 cut(s) 7, 227, 517, 597, 639
SsiI CCGC 2 cut(s) 482, 979
SspI AATATT 1 cut(s) 469
SspMI CTAG 2 cut(s) 294, 750
StyI CCWWGG 4 cut(s) 147, 161, 668, 952
TaaI ACNGT 2 cut(s) 238, 987
TaiI ACGT 1 cut(s) 368
TaqI TCGA 2 cut(s) 328, 943
TasI AATT 5 cut(s) 7, 227, 517, 597, 639
TatI WGTACW 1 cut(s) 813
TauI GCSGC 1 cut(s) 982
TfiI GAWTC 2 cut(s) 215, 701
Tru1I TTAA 2 cut(s) 555, 864
Tru9I TTAA 2 cut(s) 555, 864
TscAI CASTG 2 cut(s) 382, 550
TseFI GTSAC 1 cut(s) 153
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 4 cut(s) 466, 530, 745, 846
TspRI CASTG 2 cut(s) 382, 550
Van91I CCANNNNNTGG 1 cut(s) 797
VpaK11BI GGWCC 4 cut(s) 77, 618, 767, 781
XapI RAATTY 2 cut(s) 7, 227
XceI RCATGY 1 cut(s) 301
XmiI GTMKAC 1 cut(s) 606
XspI CTAG 2 cut(s) 294, 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.