Rw6G011350

At1g01500-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001242
Physical Location & Seq
Reverse (-)
19984731 .. 19989151
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G011350.1

Sequence Viewer

Length: 993 bp
ATGGAAAATTCTTATGAGACATCAAACAGCCACACACCTATTTTCAATGGCTATGCACCTACTTCAAATGGCCATGGACCTATTTCAAATGGTCGTGGGTCAACTGGTGGTGGCCACATGATTGGGAGACAATCCACCTACCAACCCAAGGTGTCACTACTACCTTGGCTTGATGTAAGAGTTTTCTATGTTAGGATTAGCAAATGTGAGATTGATGATTTGGCTCCAGAATTCCTCACGGTAAATCACATTCCATTGGATGCTGATACCCTTCTTGAAGTCAATGGTGTTAGAACTAGCATGTATTCAGATGGGGAAACAACTCTTCTTCGAAGAGACCGGTTAGATAAGAAGTCAGAAGAAGCTACGTTTGTCAGCACTGATAGTATAAGGATGACTGGAAGTGTGAAGTTTGAGGTTTTTGACAAGGATGTTATTGTGCTATCTGGGGTTTTAGAGTTGTGTAATAATATTGGTTTCATTGCGGAAACAGATCATCATGGCCCGAGATGGAGCATGAATTGTGAAACAAATATAATTGCAAGCACTGGCTTCTTTAAACCAAAGCAGTTTACGGGTTCAGAGTCTGCAACACCTTCAATTGAAGTATACATTGCAGGGTCCTTCTTGGGAACTCCAATAATTCTAACAAAGACTTTACAGCTGAGTCCTTGGAAGAAGCATACGAGGAAGGGGTTATTGGATTCAATACCAGAGTATGATGCAACTGAAGACGAGAACAATAATCCTTCTATGTTCATGTTTCAGGGACCAGAATACTTGGACCTGAAACCAGAATATGGAGAAAATAACTTGTACTACTCTGGGACATATTTAGAAGGTGAAGATGGAGAGCTTTCATGGTTTAATGCTGGTGTGAGAGTTGGTGTTGGGATTGGCTTGAGCATATGCGTTGGGGTTGGCATCGGAGTAGGTTTGCTTGTTCGAACTTACCAAGGCACCACTCAGAGCTTTAGAAGGCGGCTACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

330

Amino Acids

36.51

Weight (kDa)

5.17

Isoelectric Point (pI)

32.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 959
AccB7I CCANNNNNTGG 1 cut(s) 800
AccI GTMKAC 1 cut(s) 609
AciI CCGC 2 cut(s) 485, 982
AcoI YGGCCR 2 cut(s) 70, 112
AcsI RAATTY 2 cut(s) 7, 230
AcuI CTGAAG 1 cut(s) 750
AfaI GTAC 1 cut(s) 818
AfiI CCNNNNNNNGG 2 cut(s) 148, 800
AgeI ACCGGT 1 cut(s) 339
AgsI TTSAA 7 cut(s) 46, 66, 87, 278, 600, 605, 708
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AluBI AGCT 4 cut(s) 365, 664, 856, 972
AluI AGCT 4 cut(s) 365, 664, 856, 972
Alw26I GTCTC 3 cut(s) 11, 121, 330
AlwNI CAGNNNCTG 1 cut(s) 587
Ama87I CYCGRG 1 cut(s) 505
AoxI GGCC 3 cut(s) 70, 112, 502
ApoI RAATTY 2 cut(s) 7, 230
AsiGI ACCGGT 1 cut(s) 339
AspS9I GGNCC 5 cut(s) 77, 503, 621, 770, 784
AsuHPI GGTGA 1 cut(s) 854
AsuII TTCGAA 2 cut(s) 331, 946
AvaI CYCGRG 1 cut(s) 505
AvaII GGWCC 4 cut(s) 77, 621, 770, 784
BalI TGGCCA 2 cut(s) 72, 114
BanI GGYRCC 1 cut(s) 959
BbsI GAAGAC 1 cut(s) 738
BccI CCATC 3 cut(s) 305, 504, 842
BcoDI GTCTC 3 cut(s) 11, 121, 330
BfaI CTAG 1 cut(s) 297
BisI GCNGC 1 cut(s) 983
BlsI GCNGC 1 cut(s) 984
Bme18I GGWCC 4 cut(s) 77, 621, 770, 784
BmeT110I CYCGRG 1 cut(s) 505
BmgT120I GGNCC 5 cut(s) 77, 503, 621, 770, 784
BmiI GGNNCC 4 cut(s) 225, 622, 771, 961
BmsI GCATC 3 cut(s) 250, 712, 933
BpiI GAAGAC 1 cut(s) 738
BpmI CTGGAG 1 cut(s) 210
Bpu14I TTCGAA 2 cut(s) 331, 946
BpuEI CTTGAG 1 cut(s) 922
BsaI GGTCTC 1 cut(s) 330
BsaJI CCNNGG 5 cut(s) 73, 147, 164, 671, 955
BsaWI WCCGGW 1 cut(s) 339
Bsc4I CCNNNNNNNGG 2 cut(s) 148, 800
Bse118I RCCGGY 1 cut(s) 339
Bse1I ACTGG 3 cut(s) 109, 403, 553
Bse3DI GCAATG 2 cut(s) 480, 612
BseDI CCNNGG 5 cut(s) 73, 147, 164, 671, 955
BseGI GGATG 3 cut(s) 265, 399, 436
BseLI CCNNNNNNNGG 2 cut(s) 148, 800
BseMI GCAATG 2 cut(s) 480, 612
BseMII CTCAG 2 cut(s) 656, 980
BseNI ACTGG 3 cut(s) 109, 403, 553
BshFI GGCC 3 cut(s) 72, 114, 504
BshNI GGYRCC 1 cut(s) 959
BshTI ACCGGT 1 cut(s) 339
BsiHKCI CYCGRG 1 cut(s) 505
BsiSI CCGG 1 cut(s) 340
BslFI GGGAC 2 cut(s) 783, 841
BslI CCNNNNNNNGG 2 cut(s) 148, 800
BsmAI GTCTC 3 cut(s) 11, 121, 330
BsmFI GGGAC 2 cut(s) 783, 841
BsnI GGCC 3 cut(s) 72, 114, 504
Bso31I GGTCTC 1 cut(s) 330
BsoBI CYCGRG 1 cut(s) 505
Bsp119I TTCGAA 2 cut(s) 331, 946
Bsp143I GATC 1 cut(s) 493
Bsp19I CCATGG 1 cut(s) 73
BspACI CCGC 2 cut(s) 485, 982
BspANI GGCC 3 cut(s) 72, 114, 504
BspCNI CTCAG 2 cut(s) 657, 979
BspLI GGNNCC 4 cut(s) 225, 622, 771, 961
BspT104I TTCGAA 2 cut(s) 331, 946
BspT107I GGYRCC 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 330
BsrDI GCAATG 2 cut(s) 480, 612
BsrFI RCCGGY 1 cut(s) 339
BsrI ACTGG 3 cut(s) 109, 403, 553
BssAI RCCGGY 1 cut(s) 339
BssECI CCNNGG 5 cut(s) 73, 147, 164, 671, 955
BssMI GATC 1 cut(s) 493
BssNAI GTATAC 1 cut(s) 610
BssT1I CCWWGG 5 cut(s) 73, 147, 164, 671, 955
Bst1107I GTATAC 1 cut(s) 610
Bst4CI ACNGT 2 cut(s) 241, 990
Bst6I CTCTTC 2 cut(s) 328, 330
BstBI TTCGAA 2 cut(s) 331, 946
BstC8I GCNNGC 1 cut(s) 544
BstDEI CTNAG 2 cut(s) 665, 966
BstDSI CCRYGG 1 cut(s) 73
BstF5I GGATG 3 cut(s) 265, 399, 436
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 3 cut(s) 11, 121, 330
BstMBI GATC 1 cut(s) 493
BstNSI RCATGY 1 cut(s) 304
BstV2I GAAGAC 1 cut(s) 738
BstXI CCANNNNNNTGG 1 cut(s) 122
BstZ17I GTATAC 1 cut(s) 610
BsuRI GGCC 3 cut(s) 72, 114, 504
BtgI CCRYGG 1 cut(s) 73
BtsCI GGATG 3 cut(s) 265, 399, 436
BtsIMutI CAGTG 2 cut(s) 378, 546
Cac8I GCNNGC 1 cut(s) 544
CaiI CAGNNNCTG 1 cut(s) 587
Cfr10I RCCGGY 1 cut(s) 339
Cfr13I GGNCC 5 cut(s) 77, 503, 621, 770, 784
Csp6I GTAC 1 cut(s) 817
CspAI ACCGGT 1 cut(s) 339
CviAII CATG 7 cut(s) 74, 118, 301, 500, 517, 760, 861
CviQI GTAC 1 cut(s) 817
DdeI CTNAG 2 cut(s) 665, 966
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
DraI TTTAAA 1 cut(s) 559
EaeI YGGCCR 2 cut(s) 70, 112
Eam1104I CTCTTC 2 cut(s) 328, 330
EarI CTCTTC 2 cut(s) 328, 330
Eco130I CCWWGG 5 cut(s) 73, 147, 164, 671, 955
Eco31I GGTCTC 1 cut(s) 330
Eco47I GGWCC 4 cut(s) 77, 621, 770, 784
Eco57I CTGAAG 1 cut(s) 750
Eco88I CYCGRG 1 cut(s) 505
EcoO109I RGGNCCY 1 cut(s) 621
EcoRI GAATTC 1 cut(s) 230
EcoT14I CCWWGG 5 cut(s) 73, 147, 164, 671, 955
ErhI CCWWGG 5 cut(s) 73, 147, 164, 671, 955
FaeI CATG 7 cut(s) 77, 121, 304, 503, 520, 763, 864
FaqI GGGAC 2 cut(s) 783, 841
FatI CATG 7 cut(s) 73, 117, 300, 499, 516, 759, 860
FauNDI CATATG 1 cut(s) 908
FblI GTMKAC 1 cut(s) 609
Fnu4HI GCNGC 1 cut(s) 983
FokI GGATG 3 cut(s) 272, 406, 443
Fsp4HI GCNGC 1 cut(s) 983
FspBI CTAG 1 cut(s) 297
GluI GCNGC 1 cut(s) 983
GsuI CTGGAG 1 cut(s) 210
HaeIII GGCC 3 cut(s) 72, 114, 504
HapII CCGG 1 cut(s) 340
Hin1II CATG 7 cut(s) 77, 121, 304, 503, 520, 763, 864
HincII GTYRAC 1 cut(s) 102
HindII GTYRAC 1 cut(s) 102
HinfI GANTC 3 cut(s) 584, 667, 704
HpaII CCGG 1 cut(s) 340
HphI GGTGA 1 cut(s) 854
Hpy166II GTNNAC 3 cut(s) 102, 573, 610
Hpy188I TCNGA 5 cut(s) 310, 358, 583, 929, 969
Hpy188III TCNNGA 2 cut(s) 227, 275
Hpy8I GTNNAC 3 cut(s) 102, 573, 610
HpyAV CCTTC 7 cut(s) 281, 606, 634, 685, 759, 833, 972
HpyCH4III ACNGT 2 cut(s) 241, 990
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 5 cut(s) 56, 542, 590, 617, 725
HpyF3I CTNAG 2 cut(s) 665, 966
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 7 cut(s) 77, 121, 304, 503, 520, 763, 864
Kzo9I GATC 1 cut(s) 493
LmnI GCTCC 2 cut(s) 229, 513
LweI GCATC 3 cut(s) 250, 712, 933
MaeI CTAG 1 cut(s) 297
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 1 cut(s) 153
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 7 cut(s) 317, 320, 345, 371, 688, 743, 857
MfeI CAATTG 1 cut(s) 600
MlsI TGGCCA 2 cut(s) 72, 114
MluCI AATT 6 cut(s) 7, 230, 520, 537, 600, 642
MluNI TGGCCA 2 cut(s) 72, 114
MlyI GAGTC 2 cut(s) 593, 676
MnlI CCTC 3 cut(s) 245, 409, 681
Mox20I TGGCCA 2 cut(s) 72, 114
MscI TGGCCA 2 cut(s) 72, 114
MseI TTAA 2 cut(s) 558, 867
Msp20I TGGCCA 2 cut(s) 72, 114
MspA1I CMGCKG 1 cut(s) 664
MspI CCGG 1 cut(s) 340
MunI CAATTG 1 cut(s) 600
NcoI CCATGG 1 cut(s) 73
NdeI CATATG 1 cut(s) 908
NdeII GATC 1 cut(s) 493
NlaIII CATG 7 cut(s) 77, 121, 304, 503, 520, 763, 864
NlaIV GGNNCC 4 cut(s) 225, 622, 771, 961
NmuCI GTSAC 1 cut(s) 153
NspI RCATGY 1 cut(s) 304
NspV TTCGAA 2 cut(s) 331, 946
PfeI GAWTC 1 cut(s) 704
PflMI CCANNNNNTGG 1 cut(s) 800
PinAI ACCGGT 1 cut(s) 339
PkrI GCNGC 1 cut(s) 984
PleI GAGTC 2 cut(s) 592, 675
PpsI GAGTC 2 cut(s) 592, 675
PpuMI RGGWCCY 1 cut(s) 621
Psp5II RGGWCCY 1 cut(s) 621
PspN4I GGNNCC 4 cut(s) 225, 622, 771, 961
PspPI GGNCC 5 cut(s) 77, 503, 621, 770, 784
PspPPI RGGWCCY 1 cut(s) 621
PstNI CAGNNNCTG 1 cut(s) 587
PvuII CAGCTG 1 cut(s) 664
RsaI GTAC 1 cut(s) 818
RsaNI GTAC 1 cut(s) 817
SaqAI TTAA 2 cut(s) 558, 867
SatI GCNGC 1 cut(s) 983
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 5 cut(s) 77, 503, 621, 770, 784
SchI GAGTC 2 cut(s) 593, 676
SfaNI GCATC 3 cut(s) 250, 712, 933
SfuI TTCGAA 2 cut(s) 331, 946
SinI GGWCC 4 cut(s) 77, 621, 770, 784
SmlI CTYRAG 1 cut(s) 901
SmoI CTYRAG 1 cut(s) 901
Sse9I AATT 6 cut(s) 7, 230, 520, 537, 600, 642
SsiI CCGC 2 cut(s) 485, 982
SspI AATATT 1 cut(s) 472
SspMI CTAG 1 cut(s) 297
StyI CCWWGG 5 cut(s) 73, 147, 164, 671, 955
TaaI ACNGT 2 cut(s) 241, 990
TaiI ACGT 1 cut(s) 371
TaqI TCGA 2 cut(s) 331, 946
TasI AATT 6 cut(s) 7, 230, 520, 537, 600, 642
TatI WGTACW 1 cut(s) 816
TauI GCSGC 1 cut(s) 985
TfiI GAWTC 1 cut(s) 704
Tru1I TTAA 2 cut(s) 558, 867
Tru9I TTAA 2 cut(s) 558, 867
TscAI CASTG 2 cut(s) 385, 553
TseFI GTSAC 1 cut(s) 153
Tsp45I GTSAC 1 cut(s) 153
TspDTI ATGAA 4 cut(s) 469, 533, 748, 849
TspRI CASTG 2 cut(s) 385, 553
Van91I CCANNNNNTGG 1 cut(s) 800
VpaK11BI GGWCC 4 cut(s) 77, 621, 770, 784
XapI RAATTY 2 cut(s) 7, 230
XceI RCATGY 1 cut(s) 304
XmiI GTMKAC 1 cut(s) 609
XspI CTAG 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.