Rorug06G0010400

At1g01500-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001242
Physical Location & Seq
Reverse (-)
1496641 .. 1499110
2470 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0010400.1

Sequence Viewer

Length: 1323 bp
ATGGTAGACTTATTATTACAAGTTCTTCTATGGAGTTTCTATCTCCTCTCTCTTGTTCTACACCTTTTTGAAGACCTGAAAAAATTAGCCTTTTCCATAGCTCTAACTCTCAACTCTTCTCCTTCAGCATCTACTTTGTCACGGTATCTCTCTTGGGTTGGGTCTCTTTTTGCGAGAATTCAGAGCATTTACCATCACACCCAAGGCCAGATCAGGTCGAATGTAAAAAGGTGGGTTTTTGAGCAGCATGGTGAAGAAAACTATGAAGCAAAGAATTTGGCACATATCATAAGCTGCCAAGACAGTAACAGTAAGATGAAAGAAAAGAATGAAGATAAAAAAGGTATGCATGAAATACTGCATAGTTCACAAAAGAAGTCTGGTCTTTCGGGTAATAGAGGGAGTGCTATCAATAGTAAGTTGCGAATGAGGCCTGCGAAGCTGTTTGTGCCGGATTTTTGTCCAAGGCAGGAGTTCATTGAAATGGGACTAAAGAAGTTGAAAGAGGACAAAAAGGAATTTGAGGTGGAAGGGAGGGATTTCTGCTTGGCAAGTAAGAAAGGTGCTAGAAGGGAGTTCATGGAAGATGGGCACAGAGAAATGGTTGATATAATGGGAGATCCCAAGCAGGCATTTTTTGCTGTGATCGATGGGCACGGAGGTTGTGCAGCTGCTGATTATGTGGCTGAAAATTTAGGGAAGAACATTATGAAAGAGCTTGAACATGTTGGAAAGGATCAAGAACATGAATTGCAGCAGGCTATTCGCCAAGGCTACTCAGTCACAGACAAGGGCTTCCTTAGTCAGGGTGTGAGCAGTGGAGCATGTGCAGCCAGTGTGGTGTTGAAGGATGGAGTGCTACATGTAGCGAATGTAGGAGATTGTAGAGTAGTTTTGAGTAACAAAGGAGTAGCAGATGTATTAACAAAAGACCACCGTTTGAGTAGTGAAGATGAGCGTCTCCGAATCGAAAACACTGGTGGCTTTGTGCAACATTTCCGCAACGGAGTTTGGAGACTTCAAGGCTCACTTGCAGTTTCTAGAGCAATTGGGGATCAACATTTGAAAGATTGGGTCATTTCTGAACCAGAAACTAAAACGATTCCTCTAACATCAGATTGCGAGTTCTTGATAATGGCTTCGGATGGCTTGTGGGACAAGGTAGATGATCAAGAGGCAGTGAATGTGGTTTTAAAAGAGAAGGATTCACTGAAGTCTTGCAAGAAGCTTGTGAATATGTCTTCAAGCAGAGGGAGCATGGATGACATAACTATTATGGTGATCAACCTTCGAAGCTTTTTGGCAGCTGATCATCTTCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

440

Amino Acids

49.28

Weight (kDa)

6.77

Isoelectric Point (pI)

39.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 185 - 423 8.2e-55 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 6
AciI CCGC 1 cut(s) 1000
AclWI GGATC 3 cut(s) 614, 744, 1062
AcsI RAATTY 4 cut(s) 177, 274, 518, 691
AcuI CTGAAG 2 cut(s) 108, 1232
AfiI CCNNNNNNNGG 1 cut(s) 805
AflIII ACRYGT 2 cut(s) 724, 862
AgsI TTSAA 8 cut(s) 71, 482, 502, 722, 847, 1022, 1066, 1245
AluBI AGCT 8 cut(s) 101, 294, 442, 671, 718, 1228, 1296, 1307
AluI AGCT 8 cut(s) 101, 294, 442, 671, 718, 1228, 1296, 1307
Alw26I GTCTC 3 cut(s) 168, 965, 1009
AlwI GGATC 3 cut(s) 614, 744, 1062
AlwNI CAGNNNCTG 1 cut(s) 674
AoxI GGCC 2 cut(s) 205, 431
ApeKI GCWGC 7 cut(s) 244, 294, 668, 671, 754, 830, 1304
ApoI RAATTY 4 cut(s) 177, 274, 518, 691
AsuHPI GGTGA 2 cut(s) 263, 1291
AsuII TTCGAA 1 cut(s) 1291
BaeGI GKGCMC 2 cut(s) 594, 657
BbsI GAAGAC 2 cut(s) 78, 1233
BbvI GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
BccI CCATC 5 cut(s) 201, 581, 644, 845, 1139
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BclI TGATCA 3 cut(s) 1168, 1281, 1309
BcoDI GTCTC 3 cut(s) 168, 965, 1009
BfaI CTAG 2 cut(s) 567, 1041
BisI GCNGC 7 cut(s) 245, 295, 669, 672, 755, 831, 1305
BlsI GCNGC 7 cut(s) 246, 296, 670, 673, 756, 832, 1306
BmsI GCATC 1 cut(s) 137
BpiI GAAGAC 2 cut(s) 78, 1233
Bpu14I TTCGAA 1 cut(s) 1291
Bsa29I ATCGAT 1 cut(s) 648
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 202, 464, 769
Bsc4I CCNNNNNNNGG 1 cut(s) 805
Bse1I ACTGG 2 cut(s) 834, 982
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 3 cut(s) 202, 464, 769
BseGI GGATG 3 cut(s) 856, 1150, 1267
BseLI CCNNNNNNNGG 1 cut(s) 805
BseMII CTCAG 1 cut(s) 792
BseNI ACTGG 2 cut(s) 834, 982
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 2 cut(s) 594, 657
BseXI GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
BsgI GTGCAG 2 cut(s) 687, 849
BshFI GGCC 2 cut(s) 207, 433
BshVI ATCGAT 1 cut(s) 648
BsiSI CCGG 1 cut(s) 452
BslFI GGGAC 2 cut(s) 501, 1169
BslI CCNNNNNNNGG 1 cut(s) 805
BsmAI GTCTC 3 cut(s) 168, 965, 1009
BsmBI CGTCTC 1 cut(s) 965
BsmFI GGGAC 2 cut(s) 501, 1169
BsnI GGCC 2 cut(s) 207, 433
Bso31I GGTCTC 1 cut(s) 168
Bsp119I TTCGAA 1 cut(s) 1291
Bsp1286I GDGCHC 2 cut(s) 594, 657
Bsp143I GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
BspACI CCGC 1 cut(s) 1000
BspANI GGCC 2 cut(s) 207, 433
BspCNI CTCAG 1 cut(s) 791
BspDI ATCGAT 1 cut(s) 648
BspPI GGATC 3 cut(s) 614, 744, 1062
BspT104I TTCGAA 1 cut(s) 1291
BspTNI GGTCTC 1 cut(s) 168
BsrI ACTGG 2 cut(s) 834, 982
BssECI CCNNGG 3 cut(s) 202, 464, 769
BssMI GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
BssT1I CCWWGG 3 cut(s) 202, 464, 769
Bst4CI ACNGT 4 cut(s) 144, 305, 311, 938
Bst6I CTCTTC 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 638
BstBI TTCGAA 1 cut(s) 1291
BstC8I GCNNGC 3 cut(s) 435, 630, 759
BstDEI CTNAG 2 cut(s) 778, 800
BstENI CCTNNNNNAGG 1 cut(s) 803
BstF5I GGATG 3 cut(s) 856, 1150, 1267
BstKTI GATC 8 cut(s) 213, 622, 648, 739, 1057, 1171, 1284, 1312
BstMAI GTCTC 3 cut(s) 168, 965, 1009
BstMBI GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
BstMWI GCNNNNNNNGC 6 cut(s) 430, 439, 448, 638, 830, 1254
BstNSI RCATGY 3 cut(s) 728, 828, 866
BstSLI GKGCMC 2 cut(s) 594, 657
BstV1I GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
BstV2I GAAGAC 2 cut(s) 78, 1233
BstX2I RGATCY 1 cut(s) 619
BstYI RGATCY 1 cut(s) 619
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 2 cut(s) 207, 433
BsuTUI ATCGAT 1 cut(s) 648
BtsCI GGATG 3 cut(s) 856, 1150, 1267
BtsI GCAGTG 2 cut(s) 823, 1185
BtsIMutI CAGTG 5 cut(s) 823, 841, 975, 1185, 1208
Cac8I GCNNGC 3 cut(s) 435, 630, 759
CaiI CAGNNNCTG 1 cut(s) 674
ClaI ATCGAT 1 cut(s) 648
CseI GACGC 1 cut(s) 947
CviAII CATG 8 cut(s) 248, 350, 580, 725, 746, 825, 863, 1258
DdeI CTNAG 2 cut(s) 778, 800
DpnI GATC 8 cut(s) 212, 621, 647, 738, 1056, 1170, 1283, 1311
DpnII GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
DraI TTTAAA 1 cut(s) 1194
Eam1104I CTCTTC 1 cut(s) 121
EarI CTCTTC 1 cut(s) 121
Eco130I CCWWGG 3 cut(s) 202, 464, 769
Eco147I AGGCCT 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 168
Eco57I CTGAAG 2 cut(s) 108, 1232
EcoNI CCTNNNNNAGG 1 cut(s) 803
EcoRI GAATTC 1 cut(s) 177
EcoT14I CCWWGG 3 cut(s) 202, 464, 769
EcoT22I ATGCAT 1 cut(s) 351
ErhI CCWWGG 3 cut(s) 202, 464, 769
Esp3I CGTCTC 1 cut(s) 965
FaeI CATG 8 cut(s) 251, 353, 583, 728, 749, 828, 866, 1261
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FaqI GGGAC 2 cut(s) 501, 1169
FatI CATG 8 cut(s) 247, 349, 579, 724, 745, 824, 862, 1257
FbaI TGATCA 3 cut(s) 1168, 1281, 1309
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 7 cut(s) 245, 295, 669, 672, 755, 831, 1305
FokI GGATG 3 cut(s) 863, 1157, 1274
Fsp4HI GCNGC 7 cut(s) 245, 295, 669, 672, 755, 831, 1305
FspBI CTAG 2 cut(s) 567, 1041
GluI GCNGC 7 cut(s) 245, 295, 669, 672, 755, 831, 1305
HaeIII GGCC 2 cut(s) 207, 433
HapII CCGG 1 cut(s) 452
HgaI GACGC 1 cut(s) 947
Hin1II CATG 8 cut(s) 251, 353, 583, 728, 749, 828, 866, 1261
HindIII AAGCTT 2 cut(s) 1226, 1294
HinfI GANTC 3 cut(s) 966, 1102, 1205
HpaII CCGG 1 cut(s) 452
HphI GGTGA 2 cut(s) 263, 1291
Hpy166II GTNNAC 2 cut(s) 7, 368
Hpy188I TCNGA 5 cut(s) 183, 965, 1084, 1117, 1144
Hpy188III TCNNGA 4 cut(s) 740, 1041, 1129, 1172
Hpy8I GTNNAC 2 cut(s) 7, 368
HpyAV CCTTC 6 cut(s) 132, 524, 564, 841, 1195, 1298
HpyCH4III ACNGT 4 cut(s) 144, 305, 311, 938
HpyCH4V TGCA 8 cut(s) 349, 361, 668, 754, 830, 991, 1034, 1221
HpyF10VI GCNNNNNNNGC 6 cut(s) 430, 439, 448, 638, 830, 1254
HpyF3I CTNAG 2 cut(s) 778, 800
Hsp92II CATG 8 cut(s) 251, 353, 583, 728, 749, 828, 866, 1261
Ksp22I TGATCA 3 cut(s) 1168, 1281, 1309
Kzo9I GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
LmnI GCTCC 2 cut(s) 821, 1254
Lsp1109I GCAGC 7 cut(s) 256, 281, 658, 680, 766, 842, 1316
LweI GCATC 1 cut(s) 137
MaeI CTAG 2 cut(s) 567, 1041
MaeIII GTNAC 4 cut(s) 138, 305, 781, 899
MalI GATC 8 cut(s) 212, 621, 647, 738, 1056, 1170, 1283, 1311
MboI GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
MfeI CAATTG 1 cut(s) 1047
MflI RGATCY 1 cut(s) 619
MhlI GDGCHC 2 cut(s) 594, 657
MluCI AATT 7 cut(s) 83, 177, 274, 518, 691, 749, 1047
MmeI TCCRAC 1 cut(s) 709
Mph1103I ATGCAT 1 cut(s) 351
MseI TTAA 2 cut(s) 923, 1193
MslI CAYNNNNRTG 1 cut(s) 482
MspA1I CMGCKG 2 cut(s) 671, 1307
MspI CCGG 1 cut(s) 452
MunI CAATTG 1 cut(s) 1047
MwoI GCNNNNNNNGC 6 cut(s) 430, 439, 448, 638, 830, 1254
NdeII GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
NlaIII CATG 8 cut(s) 251, 353, 583, 728, 749, 828, 866, 1261
NmuCI GTSAC 2 cut(s) 138, 781
NsiI ATGCAT 1 cut(s) 351
NspI RCATGY 3 cut(s) 728, 828, 866
NspV TTCGAA 1 cut(s) 1291
PceI AGGCCT 1 cut(s) 433
PciI ACATGT 2 cut(s) 724, 862
PfeI GAWTC 3 cut(s) 966, 1102, 1205
PkrI GCNGC 7 cut(s) 246, 296, 670, 673, 756, 832, 1306
PscI ACATGT 2 cut(s) 724, 862
PstNI CAGNNNCTG 1 cut(s) 674
PsuI RGATCY 1 cut(s) 619
PvuII CAGCTG 2 cut(s) 671, 1307
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 2 cut(s) 923, 1193
SatI GCNGC 7 cut(s) 245, 295, 669, 672, 755, 831, 1305
Sau3AI GATC 8 cut(s) 210, 619, 645, 736, 1054, 1168, 1281, 1309
SduI GDGCHC 2 cut(s) 594, 657
SfaNI GCATC 1 cut(s) 137
SfuI TTCGAA 1 cut(s) 1291
SmiMI CAYNNNNRTG 1 cut(s) 482
Sse9I AATT 7 cut(s) 83, 177, 274, 518, 691, 749, 1047
SseBI AGGCCT 1 cut(s) 433
SsiI CCGC 1 cut(s) 1000
SspMI CTAG 2 cut(s) 567, 1041
StuI AGGCCT 1 cut(s) 433
StyI CCWWGG 3 cut(s) 202, 464, 769
TaaI ACNGT 4 cut(s) 144, 305, 311, 938
TaqI TCGA 4 cut(s) 218, 648, 969, 1291
TasI AATT 7 cut(s) 83, 177, 274, 518, 691, 749, 1047
TfiI GAWTC 3 cut(s) 966, 1102, 1205
Tru1I TTAA 2 cut(s) 923, 1193
Tru9I TTAA 2 cut(s) 923, 1193
TscAI CASTG 5 cut(s) 823, 841, 982, 1185, 1215
TseFI GTSAC 2 cut(s) 138, 781
TseI GCWGC 7 cut(s) 244, 294, 668, 671, 754, 830, 1304
Tsp45I GTSAC 2 cut(s) 138, 781
TspDTI ATGAA 8 cut(s) 279, 332, 345, 366, 466, 568, 725, 762
TspGWI ACGGA 3 cut(s) 672, 1020, 1308
TspRI CASTG 5 cut(s) 823, 841, 982, 1185, 1215
XagI CCTNNNNNAGG 1 cut(s) 803
XapI RAATTY 4 cut(s) 177, 274, 518, 691
XbaI TCTAGA 1 cut(s) 1040
XceI RCATGY 3 cut(s) 728, 828, 866
XmiI GTMKAC 1 cut(s) 6
XspI CTAG 2 cut(s) 567, 1041
Zsp2I ATGCAT 1 cut(s) 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.