Rmu_co8302127.1_g000001

At1g01500-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8302127.1
OG0001242
Physical Location & Seq
Reverse (-)
1 .. 758
758 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8302127.1_g000001.1.cds

Sequence Viewer

Length: 309 bp
atgtctaatagcaatggtttcgttggggaatcaaagaacaatgccaagcgatggagcatgaattgtgaatcggagatcactgctggcactggatttttcaagggaaaacaacttaatggcactgaatcacctgcaccaagcattgaggtttatgttgctggctccttctctggatcaccaatcatcttaacgaagaccctgcagattagtttccgaaagaagcacaataggaagagcactttggatgcaattccggagtatgaagcaactgaattgcagaaagatgagtctcctgaacttgatacagag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

103

Amino Acids

11.3

Weight (kDa)

5.46

Isoelectric Point (pI)

65.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 51
AccIII TCCGGA 1 cut(s) 253
AclWI GGATC 1 cut(s) 181
AfiI CCNNNNNNNGG 1 cut(s) 51
AgsI TTSAA 1 cut(s) 100
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
Alw21I GWGCWC 1 cut(s) 239
Alw26I GTCTC 1 cut(s) 294
AlwI GGATC 1 cut(s) 181
Aor13HI TCCGGA 1 cut(s) 253
AsuHPI GGTGA 2 cut(s) 120, 168
BbsI GAAGAC 1 cut(s) 200
Bbv12I GWGCWC 1 cut(s) 239
BccI CCATC 1 cut(s) 45
BcgI CGANNNNNNTGC 3 cut(s) 35, 181, 215
BcoDI GTCTC 1 cut(s) 294
BfmI CTRYAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 139
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 1 cut(s) 235
BpiI GAAGAC 1 cut(s) 200
BsaWI WCCGGW 1 cut(s) 253
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 19
BseAI TCCGGA 1 cut(s) 253
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 51
BseMI GCAATG 1 cut(s) 19
BseNI ACTGG 1 cut(s) 94
BsgI GTGCAG 1 cut(s) 117
BsiHKAI GWGCWC 1 cut(s) 239
BsiSI CCGG 1 cut(s) 254
BslI CCNNNNNNNGG 1 cut(s) 51
BsmAI GTCTC 1 cut(s) 294
Bsp1286I GDGCHC 1 cut(s) 239
Bsp13I TCCGGA 1 cut(s) 253
Bsp143I GATC 2 cut(s) 75, 173
BspEI TCCGGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 204
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 1 cut(s) 181
BspQI GCTCTTC 1 cut(s) 227
BsrDI GCAATG 1 cut(s) 19
BsrI ACTGG 1 cut(s) 94
BssMI GATC 2 cut(s) 75, 173
Bst6I CTCTTC 1 cut(s) 227
BstC8I GCNNGC 2 cut(s) 85, 160
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 2 cut(s) 78, 176
BstMAI GTCTC 1 cut(s) 294
BstMBI GATC 2 cut(s) 75, 173
BstSFI CTRYAG 1 cut(s) 200
BstV2I GAAGAC 1 cut(s) 200
BtgZI GCGATG 1 cut(s) 64
BtsCI GGATG 1 cut(s) 250
BtsI GCAGTG 1 cut(s) 78
BtsIMutI CAGTG 3 cut(s) 78, 87, 120
BveI ACCTGC 1 cut(s) 139
Cac8I GCNNGC 2 cut(s) 85, 160
CviAII CATG 1 cut(s) 58
CviJI RGCY 1 cut(s) 162
CviKI_1 RGCY 1 cut(s) 162
DpnI GATC 2 cut(s) 77, 175
DpnII GATC 2 cut(s) 75, 173
Eam1104I CTCTTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 227
FaeI CATG 1 cut(s) 61
FaiI YATR 3 cut(s) 59, 153, 261
FatI CATG 1 cut(s) 57
FokI GGATG 1 cut(s) 257
HapII CCGG 1 cut(s) 254
Hin1II CATG 1 cut(s) 61
HinfI GANTC 4 cut(s) 29, 68, 125, 287
HpaII CCGG 1 cut(s) 254
HphI GGTGA 2 cut(s) 120, 168
Hpy188I TCNGA 2 cut(s) 73, 215
Hpy188III TCNNGA 3 cut(s) 171, 254, 293
HpyAV CCTTC 1 cut(s) 175
HpyCH4V TGCA 4 cut(s) 134, 202, 248, 277
Hsp92II CATG 1 cut(s) 61
Kpn2I TCCGGA 1 cut(s) 253
Kzo9I GATC 2 cut(s) 75, 173
LguI GCTCTTC 1 cut(s) 227
LmnI GCTCC 2 cut(s) 54, 167
LpnPI CCDG 7 cut(s) 69, 75, 144, 144, 156, 212, 267
LweI GCATC 1 cut(s) 235
MalI GATC 2 cut(s) 77, 175
MboI GATC 2 cut(s) 75, 173
MboII GAAGA 2 cut(s) 205, 244
MhlI GDGCHC 1 cut(s) 239
MluCI AATT 3 cut(s) 61, 249, 272
MlyI GAGTC 1 cut(s) 296
MnlI CCTC 1 cut(s) 139
MroI TCCGGA 1 cut(s) 253
MseI TTAA 2 cut(s) 114, 188
MspI CCGG 1 cut(s) 254
NdeII GATC 2 cut(s) 75, 173
NlaIII CATG 1 cut(s) 61
NlaIV GGNNCC 1 cut(s) 163
PaqCI CACCTGC 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 227
PfeI GAWTC 3 cut(s) 29, 68, 125
PflMI CCANNNNNTGG 1 cut(s) 51
PleI GAGTC 1 cut(s) 295
PpsI GAGTC 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 163
PstI CTGCAG 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 227
SaqAI TTAA 2 cut(s) 114, 188
Sau3AI GATC 2 cut(s) 75, 173
SchI GAGTC 1 cut(s) 296
SduI GDGCHC 1 cut(s) 239
SetI ASST 2 cut(s) 133, 150
SfaNI GCATC 1 cut(s) 235
SfcI CTRYAG 1 cut(s) 200
Sse9I AATT 3 cut(s) 61, 249, 272
TasI AATT 3 cut(s) 61, 249, 272
TfiI GAWTC 3 cut(s) 29, 68, 125
Tru1I TTAA 2 cut(s) 114, 188
Tru9I TTAA 2 cut(s) 114, 188
TscAI CASTG 3 cut(s) 85, 94, 127
TspDTI ATGAA 2 cut(s) 74, 276
TspRI CASTG 3 cut(s) 85, 94, 127
Van91I CCANNNNNTGG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.