AT1G55265

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002071
Physical Location & Seq
Forward (+)
20617313 .. 20618005
693 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G55265.1

Sequence Viewer

Length: 528 bp
ATGGCTTCTTCTTTGCTTGCACTCTTCTCCTGTCTCTTCCTCTCTCTCTTATCTCTCTCCTCCTCCCTAAATCTCCGCCGTCCGATCTTCTCTCAATCCAACGACCTCGATCTCTTCTCTTCTCTAAATCTCGACCGTCCATCTCTCGCCGCCGATGACATCCACGATCTTCTCCCACGCTACGGATTCCCGAAAGGTCTTCTTCCCAACAACGTCAAATCGTACACTATCTCCGACGACGGCGATTTCACCGTTGACCTGATTTCCAGTTGCTACGTCAAGTTCTCCGATCAACTCGTTTTCTACGGCAAGAATATCGCCGGAAAACTCAGTTACGGATCTGTTAAAGACGTCCGTGGAATCCAAGCTAAAGAAGCTTTCCTTTGGCTACCAATCACCGCCATGGAATCGGATCCAAGCTCTGCCACGGTTGTGTTCTCCGTCGGATTTGTGTCCAAGACTTTACCTGCTTCCATGTTCGAAAATGTTCCTTCTTGCTCAAGAAACCTAAATCTTCAAGACTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

175

Amino Acids

19.14

Weight (kDa)

5.0

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 54 - 161 3.6e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 354
Acc36I ACCTGC 1 cut(s) 475
AciI CCGC 3 cut(s) 76, 150, 399
AclWI GGATC 3 cut(s) 346, 407, 420
AcyI GRCGYC 1 cut(s) 351
AfaI GTAC 1 cut(s) 224
AfiI CCNNNNNNNGG 1 cut(s) 182
AgsI TTSAA 1 cut(s) 518
AleI CACNNNNGTG 1 cut(s) 431
AluBI AGCT 3 cut(s) 368, 377, 420
AluI AGCT 3 cut(s) 368, 377, 420
Alw26I GTCTC 1 cut(s) 38
AlwI GGATC 3 cut(s) 346, 407, 420
Asp700I GAANNNNTTC 1 cut(s) 486
AsuHPI GGTGA 2 cut(s) 241, 388
AsuII TTCGAA 1 cut(s) 480
BamHI GGATCC 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 191
BccI CCATC 1 cut(s) 148
BceAI ACGGC 3 cut(s) 63, 256, 322
BcgI CGANNNNNNTGC 2 cut(s) 298, 332
BcoDI GTCTC 1 cut(s) 38
BfuAI ACCTGC 1 cut(s) 475
BisI GCNGC 1 cut(s) 150
BlsI GCNGC 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 414
BpiI GAAGAC 1 cut(s) 191
Bpu14I TTCGAA 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 484
BsaHI GRCGYC 1 cut(s) 351
BsaJI CCNNGG 3 cut(s) 355, 402, 426
BsaXI ACNNNNNCTCC 2 cut(s) 215, 245
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 267
BseDI CCNNGG 3 cut(s) 355, 402, 426
BseGI GGATG 1 cut(s) 159
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMII CTCAG 1 cut(s) 343
BseNI ACTGG 1 cut(s) 267
BseRI GAGGAG 2 cut(s) 49, 52
Bsh1285I CGRYCG 1 cut(s) 136
BsiEI CGRYCG 1 cut(s) 136
BsiSI CCGG 1 cut(s) 321
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 480
Bsp143I GATC 6 cut(s) 84, 109, 166, 289, 338, 412
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 3 cut(s) 76, 150, 399
BspCNI CTCAG 1 cut(s) 342
BspLI GGNNCC 1 cut(s) 414
BspMI ACCTGC 1 cut(s) 475
BspPI GGATC 3 cut(s) 346, 407, 420
BspT104I TTCGAA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 267
BssECI CCNNGG 3 cut(s) 355, 402, 426
BssMI GATC 6 cut(s) 84, 109, 166, 289, 338, 412
BssNI GRCGYC 1 cut(s) 351
BssT1I CCWWGG 1 cut(s) 402
Bst4CI ACNGT 3 cut(s) 137, 253, 430
Bst6I CTCTTC 4 cut(s) 29, 41, 119, 124
BstACI GRCGYC 1 cut(s) 351
BstBI TTCGAA 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 329
BstDSI CCRYGG 3 cut(s) 355, 402, 426
BstF5I GGATG 1 cut(s) 159
BstKTI GATC 6 cut(s) 87, 112, 169, 292, 341, 415
BstMAI GTCTC 1 cut(s) 38
BstMBI GATC 6 cut(s) 84, 109, 166, 289, 338, 412
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 1 cut(s) 374
BstV2I GAAGAC 1 cut(s) 191
BstX2I RGATCY 2 cut(s) 338, 412
BstYI RGATCY 2 cut(s) 338, 412
BtgI CCRYGG 3 cut(s) 355, 402, 426
BtsCI GGATG 1 cut(s) 159
BveI ACCTGC 1 cut(s) 475
Cac8I GCNNGC 1 cut(s) 18
Csp6I GTAC 1 cut(s) 223
CviAII CATG 2 cut(s) 403, 475
CviJI RGCY 5 cut(s) 5, 368, 377, 388, 420
CviKI_1 RGCY 5 cut(s) 5, 368, 377, 388, 420
CviQI GTAC 1 cut(s) 223
DdeI CTNAG 1 cut(s) 329
DpnI GATC 6 cut(s) 86, 111, 168, 291, 340, 414
DpnII GATC 6 cut(s) 84, 109, 166, 289, 338, 412
Eam1104I CTCTTC 4 cut(s) 29, 41, 119, 124
EarI CTCTTC 4 cut(s) 29, 41, 119, 124
EciI GGCGGA 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 402
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 2 cut(s) 406, 478
FaiI YATR 2 cut(s) 404, 476
FalI AAGNNNNNCTT 4 cut(s) 186, 218, 366, 398
FatI CATG 2 cut(s) 402, 474
Fnu4HI GCNGC 1 cut(s) 150
FokI GGATG 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 150
HapII CCGG 1 cut(s) 321
Hin1I GRCGYC 1 cut(s) 351
Hin1II CATG 2 cut(s) 406, 478
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 375
HinfI GANTC 4 cut(s) 186, 360, 407, 521
HpaII CCGG 1 cut(s) 321
HphI GGTGA 2 cut(s) 241, 388
Hpy166II GTNNAC 2 cut(s) 225, 256
Hpy188I TCNGA 5 cut(s) 84, 235, 289, 412, 446
Hpy188III TCNNGA 5 cut(s) 131, 190, 501, 518, 525
Hpy8I GTNNAC 2 cut(s) 225, 256
Hpy99I CGWCG 3 cut(s) 239, 242, 446
HpyAV CCTTC 1 cut(s) 501
HpyCH4III ACNGT 3 cut(s) 137, 253, 430
HpyCH4IV ACGT 3 cut(s) 213, 276, 351
HpyCH4V TGCA 1 cut(s) 20
HpyF10VI GCNNNNNNNGC 1 cut(s) 374
HpyF3I CTNAG 1 cut(s) 329
HpySE526I ACGT 3 cut(s) 213, 276, 351
Hsp92I GRCGYC 1 cut(s) 351
Hsp92II CATG 2 cut(s) 406, 478
Kzo9I GATC 6 cut(s) 84, 109, 166, 289, 338, 412
LpnPI CCDG 5 cut(s) 43, 272, 280, 334, 480
MaeII ACGT 3 cut(s) 213, 276, 351
MaeIII GTNAC 1 cut(s) 332
MalI GATC 6 cut(s) 86, 111, 168, 291, 340, 414
MboI GATC 6 cut(s) 84, 109, 166, 289, 338, 412
MboII GAAGA 9 cut(s) 16, 28, 79, 106, 111, 161, 191, 194, 506
MflI RGATCY 2 cut(s) 338, 412
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 3 cut(s) 123, 258, 424
MnlI CCTC 4 cut(s) 50, 70, 73, 116
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 2 cut(s) 401, 431
MspI CCGG 1 cut(s) 321
MwoI GCNNNNNNNGC 1 cut(s) 374
NcoI CCATGG 1 cut(s) 402
NdeII GATC 6 cut(s) 84, 109, 166, 289, 338, 412
NlaIII CATG 2 cut(s) 406, 478
NlaIV GGNNCC 1 cut(s) 414
NspV TTCGAA 1 cut(s) 480
OliI CACNNNNGTG 1 cut(s) 431
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 3 cut(s) 186, 360, 407
PkrI GCNGC 1 cut(s) 151
PleI GAGTC 1 cut(s) 515
PpsI GAGTC 1 cut(s) 515
PspN4I GGNNCC 1 cut(s) 414
PsuI RGATCY 2 cut(s) 338, 412
RsaI GTAC 1 cut(s) 224
RsaNI GTAC 1 cut(s) 223
RseI CAYNNNNRTG 2 cut(s) 401, 431
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 1 cut(s) 150
Sau3AI GATC 6 cut(s) 84, 109, 166, 289, 338, 412
SchI GAGTC 1 cut(s) 515
SfuI TTCGAA 1 cut(s) 480
SmiMI CAYNNNNRTG 2 cut(s) 401, 431
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
SsiI CCGC 3 cut(s) 76, 150, 399
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 3 cut(s) 137, 253, 430
TaiI ACGT 3 cut(s) 216, 279, 354
TaqI TCGA 3 cut(s) 108, 132, 480
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 3 cut(s) 186, 360, 407
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TspGWI ACGGA 4 cut(s) 198, 344, 351, 430
XmnI GAANNNNTTC 1 cut(s) 486
ZraI GACGTC 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.