RchiOBHm_Chr5g0082171

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002071
Physical Location & Seq
Forward (+)
88142130 .. 88144288
2159 bp
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UTR
Exon/CDS
Intron
PRQ35639

Sequence Viewer

Length: 519 bp
ATGGCCTCGTCCATCATCCAAATTTCTCTCTACCTTCTACTCCTATCCCTCTTCTCAGAAACCCAATTATCTCAATCCCTCAGAGACCTCAAACCCAACCCCAACGGCCCATCCACCTCGCTCCAATCAATCACAGACGTCCATGACCTCCTCCCCAAGTATGGCCTCCCAAAGGGTCTGTTACCCAACAACGTAAGATCCTACACTCTCTCCGACGATGGAACCTTCGAGATCCATCTCGAAAACCCATGTTATGTTCACTTTGATCAGTTGGTGTACTACAACAAGAACATCAAAGGGAAGCTGAGTTTCGGCTCTGTTTCCGATGTGTCGGGGATTCAAGCCAAGAAGCTATTTATTTGGGTTTCCGTTACGGGGATGCATATGGAACAAGGGTCTGATTCAGTTGAGTTTTACGTTGGGGCTCTGTCCGAGAAATTGCCTGCTAAACAGTTCGAGGATGTTCCTGTATGTAAGAGCAAGGCTTGCCGAGGAGGAGCTAGTGTTGAGTCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

172

Amino Acids

18.94

Weight (kDa)

6.06

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 47 - 153 8.8e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AclWI GGATC 2 cut(s) 192, 226
AcsI RAATTY 1 cut(s) 21
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 278
AfiI CCNNNNNNNGG 3 cut(s) 161, 172, 375
AgsI TTSAA 1 cut(s) 341
AluBI AGCT 3 cut(s) 304, 352, 500
AluI AGCT 3 cut(s) 304, 352, 500
Alw26I GTCTC 1 cut(s) 78
AlwI GGATC 2 cut(s) 192, 226
AoxI GGCC 3 cut(s) 3, 106, 163
ApoI RAATTY 1 cut(s) 21
AspS9I GGNCC 1 cut(s) 107
BanII GRGCYC 1 cut(s) 427
BccI CCATC 4 cut(s) 20, 118, 212, 243
BceAI ACGGC 1 cut(s) 121
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 1 cut(s) 501
BmgT120I GGNCC 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 223
BmsI GCATC 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 490
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 489, 519
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 172, 375
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 4 cut(s) 15, 110, 384, 466
BseLI CCNNNNNNNGG 3 cut(s) 161, 172, 375
BseMII CTCAG 3 cut(s) 69, 94, 296
BseRI GAGGAG 3 cut(s) 140, 507, 510
BshFI GGCC 3 cut(s) 5, 108, 165
BslI CCNNNNNNNGG 3 cut(s) 161, 172, 375
BsmAI GTCTC 1 cut(s) 78
BsnI GGCC 3 cut(s) 5, 108, 165
Bso31I GGTCTC 1 cut(s) 78
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 3 cut(s) 197, 231, 265
BspANI GGCC 3 cut(s) 5, 108, 165
BspCNI CTCAG 3 cut(s) 68, 93, 297
BspLI GGNNCC 1 cut(s) 223
BspPI GGATC 2 cut(s) 192, 226
BspTNI GGTCTC 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 3 cut(s) 197, 231, 265
BssNI GRCGYC 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 453
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 444, 487
BstDEI CTNAG 3 cut(s) 55, 80, 305
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 4 cut(s) 15, 110, 384, 466
BstKTI GATC 3 cut(s) 200, 234, 268
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 3 cut(s) 197, 231, 265
BstMWI GCNNNNNNNGC 1 cut(s) 486
BstX2I RGATCY 2 cut(s) 197, 231
BstYI RGATCY 2 cut(s) 197, 231
BsuRI GGCC 3 cut(s) 5, 108, 165
BtsCI GGATG 4 cut(s) 15, 110, 384, 466
Cac8I GCNNGC 2 cut(s) 444, 487
Cfr13I GGNCC 1 cut(s) 107
Csp6I GTAC 1 cut(s) 277
CviAII CATG 2 cut(s) 143, 249
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 3 cut(s) 55, 80, 305
DpnI GATC 3 cut(s) 199, 233, 267
DpnII GATC 3 cut(s) 197, 231, 265
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco24I GRGCYC 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoT22I ATGCAT 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 427
FaeI CATG 2 cut(s) 146, 252
FaiI YATR 7 cut(s) 144, 162, 250, 255, 384, 386, 472
FatI CATG 2 cut(s) 142, 248
FauNDI CATATG 1 cut(s) 384
FbaI TGATCA 1 cut(s) 265
FokI GGATG 4 cut(s) 2, 97, 391, 473
FriOI GRGCYC 1 cut(s) 427
FspBI CTAG 1 cut(s) 501
HaeIII GGCC 3 cut(s) 5, 108, 165
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 2 cut(s) 146, 252
HinfI GANTC 3 cut(s) 337, 401, 509
Hpy166II GTNNAC 2 cut(s) 259, 277
Hpy188I TCNGA 6 cut(s) 58, 83, 214, 325, 400, 433
Hpy188III TCNNGA 2 cut(s) 229, 239
Hpy8I GTNNAC 2 cut(s) 259, 277
Hpy99I CGWCG 1 cut(s) 218
HpyAV CCTTC 2 cut(s) 44, 235
HpyCH4III ACNGT 1 cut(s) 453
HpyCH4IV ACGT 3 cut(s) 138, 192, 417
HpyCH4V TGCA 1 cut(s) 382
HpyF10VI GCNNNNNNNGC 1 cut(s) 486
HpyF3I CTNAG 3 cut(s) 55, 80, 305
HpySE526I ACGT 3 cut(s) 138, 192, 417
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 2 cut(s) 146, 252
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 3 cut(s) 197, 231, 265
LmnI GCTCC 2 cut(s) 126, 497
LpnPI CCDG 2 cut(s) 456, 480
LweI GCATC 1 cut(s) 369
MaeI CTAG 1 cut(s) 501
MaeII ACGT 3 cut(s) 138, 192, 417
MaeIII GTNAC 2 cut(s) 180, 370
MalI GATC 3 cut(s) 199, 233, 267
MboI GATC 3 cut(s) 197, 231, 265
MboII GAAGA 1 cut(s) 43
MflI RGATCY 2 cut(s) 197, 231
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 3 cut(s) 21, 65, 437
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 237
Mph1103I ATGCAT 1 cut(s) 384
MwoI GCNNNNNNNGC 1 cut(s) 486
NdeI CATATG 1 cut(s) 384
NdeII GATC 3 cut(s) 197, 231, 265
NlaIII CATG 2 cut(s) 146, 252
NlaIV GGNNCC 1 cut(s) 223
NmeAIII GCCGAG 1 cut(s) 515
NsiI ATGCAT 1 cut(s) 384
PfeI GAWTC 2 cut(s) 337, 401
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 107
PsuI RGATCY 2 cut(s) 197, 231
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
Sau3AI GATC 3 cut(s) 197, 231, 265
Sau96I GGNCC 1 cut(s) 107
SchI GAGTC 1 cut(s) 518
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 1 cut(s) 369
Sse9I AATT 3 cut(s) 21, 65, 437
SspMI CTAG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 453
TaiI ACGT 3 cut(s) 141, 195, 420
TaqI TCGA 3 cut(s) 228, 240, 456
TasI AATT 3 cut(s) 21, 65, 437
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 2 cut(s) 337, 401
TspGWI ACGGA 1 cut(s) 358
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 1 cut(s) 21
XspI CTAG 1 cut(s) 501
ZraI GACGTC 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.