Rh5DG565500

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002071
Physical Location & Seq
Forward (+)
87217657 .. 87321283
103627 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG565500.1

Sequence Viewer

Length: 552 bp
ATGGAGTACAGGAAGCTCAAAGATCAGGATGAAAATGGAAATTCAGCTGCCGACGACATAAAAAATTTATGGGGAAATCCGCTTTCTATTGAAAGCCAATTATCTCAATCCCTCAGAGACCTCAAACCCAGCCCCAACCGTCCATCCACCTCGCTCCAATCAATCACAGATGTCCATGACCTCCTCCCCAAGTTTGGCCTCCCAAGGGGTCTGTTACCCGACAACGTAAGATCCTACACTCTATCTGACGATGGAACCTTTGAGATCCATCTCGAAAACCCATGTTATGTTCACTTTGATCAGTTGGTGTACTACAACAAGAACATCAAAGGGAAGCTGAGTTTCGGCTCTGTTTCCGATGTGTCGGGGATTCAAGCCAAGAAGCTATTTATTTGGGTTTCCGTGACGGGGATGCATATGGAACAAGGGTCTGATTCGGTTGAGTTTTACGTTGGGGCTCTGTCCGAGAAATTGCCTGCTAAACAGTTCGAGGACATTCCTGTATGTAAGAGCAAGGCTTGCCGAGGAGGAGCTAGTGTGGAGTCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

183

Amino Acids

20.34

Weight (kDa)

5.56

Isoelectric Point (pI)

44.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 58 - 164 2.7e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 80
AclWI GGATC 2 cut(s) 225, 259
AcsI RAATTY 2 cut(s) 40, 64
AfaI GTAC 2 cut(s) 8, 311
AfiI CCNNNNNNNGG 3 cut(s) 194, 205, 408
AgsI TTSAA 2 cut(s) 92, 374
AluBI AGCT 5 cut(s) 16, 47, 337, 385, 533
AluI AGCT 5 cut(s) 16, 47, 337, 385, 533
Alw26I GTCTC 1 cut(s) 111
AlwI GGATC 2 cut(s) 225, 259
AoxI GGCC 1 cut(s) 196
ApeKI GCWGC 1 cut(s) 47
ApoI RAATTY 2 cut(s) 40, 64
BanII GRGCYC 1 cut(s) 460
BbvI GCAGC 1 cut(s) 34
BccI CCATC 3 cut(s) 151, 245, 276
BclI TGATCA 1 cut(s) 298
BcoDI GTCTC 1 cut(s) 111
BfaI CTAG 1 cut(s) 534
BisI GCNGC 1 cut(s) 48
BlsI GCNGC 1 cut(s) 49
BmiI GGNNCC 1 cut(s) 256
BmsI GCATC 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 203, 523
BsaXI ACNNNNNCTCC 3 cut(s) 522, 533, 552
Bsc4I CCNNNNNNNGG 3 cut(s) 194, 205, 408
BseDI CCNNGG 2 cut(s) 203, 523
BseGI GGATG 3 cut(s) 34, 143, 417
BseLI CCNNNNNNNGG 3 cut(s) 194, 205, 408
BseMII CTCAG 2 cut(s) 127, 329
BseRI GAGGAG 3 cut(s) 173, 540, 543
BseXI GCAGC 1 cut(s) 34
BseYI CCCAGC 1 cut(s) 128
BshFI GGCC 1 cut(s) 198
BslI CCNNNNNNNGG 3 cut(s) 194, 205, 408
BsmAI GTCTC 1 cut(s) 111
BsnI GGCC 1 cut(s) 198
Bso31I GGTCTC 1 cut(s) 111
Bsp1286I GDGCHC 1 cut(s) 460
Bsp143I GATC 4 cut(s) 22, 230, 264, 298
BspACI CCGC 1 cut(s) 80
BspANI GGCC 1 cut(s) 198
BspCNI CTCAG 2 cut(s) 126, 330
BspLI GGNNCC 1 cut(s) 256
BspPI GGATC 2 cut(s) 225, 259
BspTNI GGTCTC 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 203, 523
BssMI GATC 4 cut(s) 22, 230, 264, 298
BssT1I CCWWGG 1 cut(s) 203
Bst4CI ACNGT 2 cut(s) 140, 486
BstAPI GCANNNNNTGC 1 cut(s) 519
BstC8I GCNNGC 2 cut(s) 477, 520
BstDEI CTNAG 2 cut(s) 113, 338
BstF5I GGATG 3 cut(s) 34, 143, 417
BstKTI GATC 4 cut(s) 25, 233, 267, 301
BstMAI GTCTC 1 cut(s) 111
BstMBI GATC 4 cut(s) 22, 230, 264, 298
BstMWI GCNNNNNNNGC 1 cut(s) 519
BstV1I GCAGC 1 cut(s) 34
BstX2I RGATCY 2 cut(s) 230, 264
BstYI RGATCY 2 cut(s) 230, 264
BsuRI GGCC 1 cut(s) 198
BtsCI GGATG 3 cut(s) 34, 143, 417
Cac8I GCNNGC 2 cut(s) 477, 520
Csp6I GTAC 2 cut(s) 7, 310
CviAII CATG 2 cut(s) 176, 282
CviQI GTAC 2 cut(s) 7, 310
DdeI CTNAG 2 cut(s) 113, 338
DpnI GATC 4 cut(s) 24, 232, 266, 300
DpnII GATC 4 cut(s) 22, 230, 264, 298
Eco130I CCWWGG 1 cut(s) 203
Eco24I GRGCYC 1 cut(s) 460
Eco31I GGTCTC 1 cut(s) 111
EcoT14I CCWWGG 1 cut(s) 203
EcoT22I ATGCAT 1 cut(s) 417
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 2 cut(s) 179, 285
FaiI YATR 8 cut(s) 59, 70, 177, 283, 288, 417, 419, 505
FatI CATG 2 cut(s) 175, 281
FauNDI CATATG 1 cut(s) 417
FbaI TGATCA 1 cut(s) 298
Fnu4HI GCNGC 1 cut(s) 48
FokI GGATG 3 cut(s) 41, 130, 424
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 1 cut(s) 48
FspBI CTAG 1 cut(s) 534
GluI GCNGC 1 cut(s) 48
GsaI CCCAGC 1 cut(s) 132
HaeIII GGCC 1 cut(s) 198
Hin1II CATG 2 cut(s) 179, 285
HinfI GANTC 3 cut(s) 370, 434, 542
Hpy166II GTNNAC 2 cut(s) 292, 310
Hpy188I TCNGA 5 cut(s) 116, 247, 358, 433, 466
Hpy188III TCNNGA 2 cut(s) 26, 272
Hpy8I GTNNAC 2 cut(s) 292, 310
Hpy99I CGWCG 1 cut(s) 56
HpyCH4III ACNGT 2 cut(s) 140, 486
HpyCH4IV ACGT 2 cut(s) 225, 450
HpyCH4V TGCA 1 cut(s) 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 519
HpyF3I CTNAG 2 cut(s) 113, 338
HpySE526I ACGT 2 cut(s) 225, 450
Hsp92II CATG 2 cut(s) 179, 285
Ksp22I TGATCA 1 cut(s) 298
Kzo9I GATC 4 cut(s) 22, 230, 264, 298
LmnI GCTCC 2 cut(s) 159, 530
LpnPI CCDG 4 cut(s) 11, 142, 489, 513
Lsp1109I GCAGC 1 cut(s) 34
LweI GCATC 1 cut(s) 402
MaeI CTAG 1 cut(s) 534
MaeII ACGT 2 cut(s) 225, 450
MaeIII GTNAC 2 cut(s) 213, 403
MalI GATC 4 cut(s) 24, 232, 266, 300
MboI GATC 4 cut(s) 22, 230, 264, 298
MflI RGATCY 2 cut(s) 230, 264
MhlI GDGCHC 1 cut(s) 460
MluCI AATT 4 cut(s) 40, 64, 98, 470
MlyI GAGTC 1 cut(s) 551
MnlI CCTC 9 cut(s) 122, 131, 160, 191, 194, 209, 484, 518, 521
Mph1103I ATGCAT 1 cut(s) 417
MspA1I CMGCKG 1 cut(s) 47
MwoI GCNNNNNNNGC 1 cut(s) 519
NdeI CATATG 1 cut(s) 417
NdeII GATC 4 cut(s) 22, 230, 264, 298
NlaIII CATG 2 cut(s) 179, 285
NlaIV GGNNCC 1 cut(s) 256
NmeAIII GCCGAG 1 cut(s) 548
NmuCI GTSAC 1 cut(s) 403
NsiI ATGCAT 1 cut(s) 417
PfeI GAWTC 2 cut(s) 370, 434
PkrI GCNGC 1 cut(s) 49
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
PspFI CCCAGC 1 cut(s) 128
PspN4I GGNNCC 1 cut(s) 256
PsuI RGATCY 2 cut(s) 230, 264
PvuII CAGCTG 1 cut(s) 47
RsaI GTAC 2 cut(s) 8, 311
RsaNI GTAC 2 cut(s) 7, 310
SatI GCNGC 1 cut(s) 48
Sau3AI GATC 4 cut(s) 22, 230, 264, 298
SchI GAGTC 1 cut(s) 551
SduI GDGCHC 1 cut(s) 460
SfaNI GCATC 1 cut(s) 402
Sse9I AATT 4 cut(s) 40, 64, 98, 470
SsiI CCGC 1 cut(s) 80
SspMI CTAG 1 cut(s) 534
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 2 cut(s) 140, 486
TaiI ACGT 2 cut(s) 228, 453
TaqI TCGA 2 cut(s) 273, 489
TasI AATT 4 cut(s) 40, 64, 98, 470
TatI WGTACW 2 cut(s) 6, 309
TfiI GAWTC 2 cut(s) 370, 434
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 47
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 1 cut(s) 45
TspGWI ACGGA 1 cut(s) 391
XapI RAATTY 2 cut(s) 40, 64
XspI CTAG 1 cut(s) 534
Zsp2I ATGCAT 1 cut(s) 417
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.