Rh5BG554600

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002071
Physical Location & Seq
Forward (+)
88130430 .. 88130948
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG554600.1

Sequence Viewer

Length: 519 bp
ATGGCCTCATCCATCGCCCAAATCTCTCTCTACCTTCTACTCCTAACCCTCTTCTCAGAAACCCAATTATCTCTATCCCTCAGAGACCTCAAACCCAACCCCAACCGTCCATCCACCTCGCTCCAATCAATCACGGACGTCCATGACCTCCTCCCCAAGTACGGCCTCCCAAGGGGTCTGTTACCCGACAACGTAAGGTCCTACACTCTCTCCGACAATGGAACCTTCGAGATCTATCTCGAGAACCAATGTTATGTTCACTTTGATCAGTTGGTGTACTACAACAAGAACATTAAAGGGAAGCTGAGTTTCGGCTCTGTTTCCGATGTGTCGGGGATTCAAGCCAAGAAGCTATTTATTTGGGTTACCGTGACGGGGATGCATATGGAACAAGGGTCTGATTCGGTTGAGTTTTACGTTGGGGCTCTGTCCGAGAAATTGCCTGCTAAACAGTTCGAGGATATTCCTGTATGTAAGAGCAAGGCTTGCAGGGGAGGAGCTAGTGTTGAGTCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

172

Amino Acids

19.11

Weight (kDa)

6.28

Isoelectric Point (pI)

37.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 47 - 153 1.3e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 2 cut(s) 161, 278
AfiI CCNNNNNNNGG 3 cut(s) 161, 172, 375
AgsI TTSAA 1 cut(s) 341
AjuI GAANNNNNNNTTGG 2 cut(s) 240, 272
AluBI AGCT 3 cut(s) 304, 352, 500
AluI AGCT 3 cut(s) 304, 352, 500
Alw26I GTCTC 1 cut(s) 78
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 2 cut(s) 3, 163
AspS9I GGNCC 1 cut(s) 198
AvaI CYCGRG 1 cut(s) 239
AvaII GGWCC 1 cut(s) 198
BanII GRGCYC 1 cut(s) 427
BccI CCATC 2 cut(s) 20, 118
BceAI ACGGC 1 cut(s) 178
BclI TGATCA 1 cut(s) 265
BcoDI GTCTC 1 cut(s) 78
BfaI CTAG 1 cut(s) 501
BglII AGATCT 1 cut(s) 231
Bme18I GGWCC 1 cut(s) 198
BmeT110I CYCGRG 1 cut(s) 239
BmgT120I GGNCC 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 223
BmsI GCATC 1 cut(s) 369
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 170
BsaXI ACNNNNNCTCC 4 cut(s) 194, 224, 489, 519
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 172, 375
BseDI CCNNGG 1 cut(s) 170
BseGI GGATG 3 cut(s) 8, 110, 384
BseLI CCNNNNNNNGG 3 cut(s) 161, 172, 375
BseMII CTCAG 3 cut(s) 69, 94, 296
BseRI GAGGAG 2 cut(s) 140, 510
BshFI GGCC 2 cut(s) 5, 165
BsiHKCI CYCGRG 1 cut(s) 239
BslI CCNNNNNNNGG 3 cut(s) 161, 172, 375
BsmAI GTCTC 1 cut(s) 78
BsnI GGCC 2 cut(s) 5, 165
Bso31I GGTCTC 1 cut(s) 78
BsoBI CYCGRG 1 cut(s) 239
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 2 cut(s) 231, 265
BspANI GGCC 2 cut(s) 5, 165
BspCNI CTCAG 3 cut(s) 68, 93, 297
BspLI GGNNCC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 170
BssMI GATC 2 cut(s) 231, 265
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 170
Bst4CI ACNGT 3 cut(s) 107, 370, 453
Bst6I CTCTTC 1 cut(s) 56
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 1 cut(s) 486
BstC8I GCNNGC 2 cut(s) 444, 487
BstDEI CTNAG 3 cut(s) 55, 80, 305
BstEII GGTNACC 1 cut(s) 364
BstF5I GGATG 3 cut(s) 8, 110, 384
BstKTI GATC 2 cut(s) 234, 268
BstMAI GTCTC 1 cut(s) 78
BstMBI GATC 2 cut(s) 231, 265
BstMWI GCNNNNNNNGC 1 cut(s) 486
BstPI GGTNACC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 231
BstYI RGATCY 1 cut(s) 231
BsuRI GGCC 2 cut(s) 5, 165
BtsCI GGATG 3 cut(s) 8, 110, 384
Cac8I GCNNGC 2 cut(s) 444, 487
Cfr13I GGNCC 1 cut(s) 198
Csp6I GTAC 2 cut(s) 160, 277
CviAII CATG 1 cut(s) 143
CviJI RGCY 9 cut(s) 5, 165, 304, 315, 344, 352, 425, 485, 500
CviKI_1 RGCY 9 cut(s) 5, 165, 304, 315, 344, 352, 425, 485, 500
CviQI GTAC 2 cut(s) 160, 277
DdeI CTNAG 3 cut(s) 55, 80, 305
DpnI GATC 2 cut(s) 233, 267
DpnII GATC 2 cut(s) 231, 265
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
Eco130I CCWWGG 1 cut(s) 170
Eco24I GRGCYC 1 cut(s) 427
Eco31I GGTCTC 1 cut(s) 78
Eco47I GGWCC 1 cut(s) 198
Eco88I CYCGRG 1 cut(s) 239
Eco91I GGTNACC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 198
EcoO65I GGTNACC 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 170
EcoT22I ATGCAT 1 cut(s) 384
EcoT38I GRGCYC 1 cut(s) 427
ErhI CCWWGG 1 cut(s) 170
FaeI CATG 1 cut(s) 146
FaiI YATR 5 cut(s) 144, 255, 384, 386, 472
FatI CATG 1 cut(s) 142
FauNDI CATATG 1 cut(s) 384
FbaI TGATCA 1 cut(s) 265
FokI GGATG 2 cut(s) 97, 391
FriOI GRGCYC 1 cut(s) 427
FspBI CTAG 1 cut(s) 501
HaeIII GGCC 2 cut(s) 5, 165
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 1 cut(s) 146
HinfI GANTC 3 cut(s) 337, 401, 509
Hpy166II GTNNAC 2 cut(s) 259, 277
Hpy188I TCNGA 6 cut(s) 58, 83, 214, 325, 400, 433
Hpy188III TCNNGA 3 cut(s) 229, 239, 241
Hpy8I GTNNAC 2 cut(s) 259, 277
HpyAV CCTTC 2 cut(s) 44, 235
HpyCH4III ACNGT 3 cut(s) 107, 370, 453
HpyCH4IV ACGT 3 cut(s) 138, 192, 417
HpyCH4V TGCA 2 cut(s) 382, 489
HpyF10VI GCNNNNNNNGC 1 cut(s) 486
HpyF3I CTNAG 3 cut(s) 55, 80, 305
HpySE526I ACGT 3 cut(s) 138, 192, 417
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 1 cut(s) 146
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 2 cut(s) 231, 265
LmnI GCTCC 2 cut(s) 126, 497
LpnPI CCDG 3 cut(s) 456, 475, 480
LweI GCATC 1 cut(s) 369
MaeI CTAG 1 cut(s) 501
MaeII ACGT 3 cut(s) 138, 192, 417
MaeIII GTNAC 3 cut(s) 180, 364, 370
MalI GATC 2 cut(s) 233, 267
MboI GATC 2 cut(s) 231, 265
MboII GAAGA 1 cut(s) 43
MflI RGATCY 1 cut(s) 231
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 2 cut(s) 65, 437
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 1 cut(s) 237
Mph1103I ATGCAT 1 cut(s) 384
MseI TTAA 1 cut(s) 294
MwoI GCNNNNNNNGC 1 cut(s) 486
NdeI CATATG 1 cut(s) 384
NdeII GATC 2 cut(s) 231, 265
NlaIII CATG 1 cut(s) 146
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 1 cut(s) 370
NsiI ATGCAT 1 cut(s) 384
PaeR7I CTCGAG 1 cut(s) 239
PfeI GAWTC 2 cut(s) 337, 401
PleI GAGTC 1 cut(s) 517
PpsI GAGTC 1 cut(s) 517
PpuMI RGGWCCY 1 cut(s) 198
Psp5II RGGWCCY 1 cut(s) 198
PspEI GGTNACC 1 cut(s) 364
PspN4I GGNNCC 1 cut(s) 223
PspPI GGNCC 1 cut(s) 198
PspPPI RGGWCCY 1 cut(s) 198
PsuI RGATCY 1 cut(s) 231
RsaI GTAC 2 cut(s) 161, 278
RsaNI GTAC 2 cut(s) 160, 277
SaqAI TTAA 1 cut(s) 294
Sau3AI GATC 2 cut(s) 231, 265
Sau96I GGNCC 1 cut(s) 198
SchI GAGTC 1 cut(s) 518
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 1 cut(s) 369
Sfr274I CTCGAG 1 cut(s) 239
SinI GGWCC 1 cut(s) 198
SlaI CTCGAG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
Sse9I AATT 2 cut(s) 65, 437
SspMI CTAG 1 cut(s) 501
StyI CCWWGG 1 cut(s) 170
TaaI ACNGT 3 cut(s) 107, 370, 453
TaiI ACGT 3 cut(s) 141, 195, 420
TaqI TCGA 3 cut(s) 228, 240, 456
TasI AATT 2 cut(s) 65, 437
TatI WGTACW 1 cut(s) 276
TfiI GAWTC 2 cut(s) 337, 401
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseFI GTSAC 1 cut(s) 370
Tsp45I GTSAC 1 cut(s) 370
TspGWI ACGGA 1 cut(s) 149
VpaK11BI GGWCC 1 cut(s) 198
XhoI CTCGAG 1 cut(s) 239
XspI CTAG 1 cut(s) 501
ZraI GACGTC 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.