Rh5DG564600

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0002071
Physical Location & Seq
Forward (+)
87011406 .. 87018922
7517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG564600.1

Sequence Viewer

Length: 402 bp
ATGGCTCAAATGATGAGCCACGAACAACTCCCGATCCGAATCCACTTCGCTCTCGACGAAGTCTACGACGATTCCTCGCAACTCGAGGAGGCCCAGCTCCGCTTACACCGCCTCAAGACCAAGTTTCACAAGGTCTTCGGGCACCTTCCTCAATTGGTGTACTACAACAAGAACATCAAAGGGAAGCTGAGTTTCGGCTCTGTTTCCGATGTGTCGGGGATTCAAGCCAAGAAGCTGTTTATTTGGGTTACCGTGACGGGGATGCATATGGAACAAGGGTCTGATTCAGTTGAGTTTTACGTTGGGGCTCTGTCCGAGAAATTGCCTGCTAAACAGTTCGAGGATATTCCGGTATGTAAGAGCAAGGCTTGCCGAGGAGGAGCTAGTGTTGAGTCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

133

Amino Acids

15.01

Weight (kDa)

7.81

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 31 - 114 1.6e-16 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccI GTMKAC 1 cut(s) 63
AciI CCGC 2 cut(s) 100, 109
AclWI GGATC 1 cut(s) 28
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 1 cut(s) 224
AluBI AGCT 4 cut(s) 97, 187, 235, 383
AluI AGCT 4 cut(s) 97, 187, 235, 383
AlwI GGATC 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 90
AspS9I GGNCC 1 cut(s) 91
AvaI CYCGRG 1 cut(s) 83
BaeGI GKGCMC 1 cut(s) 144
BanI GGYRCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 310
BbsI GAAGAC 1 cut(s) 127
BfaI CTAG 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 143
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 127
BpuEI CTTGAG 1 cut(s) 98
BsaBI GATNNNNATC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 373
BsaWI WCCGGW 1 cut(s) 349
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse8I GATNNNNATC 1 cut(s) 38
BseDI CCNNGG 1 cut(s) 373
BseGI GGATG 1 cut(s) 267
BseJI GATNNNNATC 1 cut(s) 38
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 179
BseRI GAGGAG 3 cut(s) 101, 390, 393
BseSI GKGCMC 1 cut(s) 144
BseYI CCCAGC 1 cut(s) 93
BshFI GGCC 1 cut(s) 92
BshNI GGYRCC 1 cut(s) 141
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 1 cut(s) 350
BslI CCNNNNNNNGG 1 cut(s) 258
BsnI GGCC 1 cut(s) 92
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 2 cut(s) 144, 310
Bsp143I GATC 1 cut(s) 33
BspACI CCGC 2 cut(s) 100, 109
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 141
BssECI CCNNGG 1 cut(s) 373
BssMI GATC 1 cut(s) 33
Bst4CI ACNGT 2 cut(s) 253, 336
BstAPI GCANNNNNTGC 1 cut(s) 369
BstC8I GCNNGC 2 cut(s) 327, 370
BstDEI CTNAG 1 cut(s) 188
BstEII GGTNACC 1 cut(s) 247
BstF5I GGATG 1 cut(s) 267
BstKTI GATC 1 cut(s) 36
BstMBI GATC 1 cut(s) 33
BstMWI GCNNNNNNNGC 2 cut(s) 108, 369
BstPI GGTNACC 1 cut(s) 247
BstSLI GKGCMC 1 cut(s) 144
BstV2I GAAGAC 1 cut(s) 127
BsuRI GGCC 1 cut(s) 92
BtsCI GGATG 1 cut(s) 267
Cac8I GCNNGC 2 cut(s) 327, 370
Cfr13I GGNCC 1 cut(s) 91
Csp6I GTAC 1 cut(s) 160
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 1 cut(s) 188
DpnI GATC 1 cut(s) 35
DpnII GATC 1 cut(s) 33
Eco24I GRGCYC 1 cut(s) 310
Eco88I CYCGRG 1 cut(s) 83
Eco91I GGTNACC 1 cut(s) 247
EcoO65I GGTNACC 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 310
FaiI YATR 3 cut(s) 267, 269, 355
FauNDI CATATG 1 cut(s) 267
FblI GTMKAC 1 cut(s) 63
FokI GGATG 1 cut(s) 274
FriOI GRGCYC 1 cut(s) 310
FspBI CTAG 1 cut(s) 384
GsaI CCCAGC 1 cut(s) 97
HaeIII GGCC 1 cut(s) 92
HapII CCGG 1 cut(s) 350
HinfI GANTC 5 cut(s) 39, 71, 220, 284, 392
HpaII CCGG 1 cut(s) 350
Hpy166II GTNNAC 2 cut(s) 64, 160
Hpy188I TCNGA 4 cut(s) 38, 208, 283, 316
Hpy188III TCNNGA 3 cut(s) 31, 53, 115
Hpy8I GTNNAC 2 cut(s) 64, 160
Hpy99I CGWCG 2 cut(s) 59, 71
HpyAV CCTTC 1 cut(s) 155
HpyCH4III ACNGT 2 cut(s) 253, 336
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 1 cut(s) 265
HpyF10VI GCNNNNNNNGC 2 cut(s) 108, 369
HpyF3I CTNAG 1 cut(s) 188
HpySE526I ACGT 1 cut(s) 300
Kzo9I GATC 1 cut(s) 33
LmnI GCTCC 2 cut(s) 102, 380
LpnPI CCDG 3 cut(s) 107, 339, 363
LweI GCATC 1 cut(s) 252
MaeI CTAG 1 cut(s) 384
MaeII ACGT 1 cut(s) 300
MaeIII GTNAC 2 cut(s) 247, 253
MalI GATC 1 cut(s) 35
MboI GATC 1 cut(s) 33
MboII GAAGA 1 cut(s) 127
MfeI CAATTG 1 cut(s) 152
MhlI GDGCHC 2 cut(s) 144, 310
MluCI AATT 2 cut(s) 152, 320
MlyI GAGTC 1 cut(s) 401
MnlI CCTC 8 cut(s) 79, 82, 85, 122, 159, 334, 368, 371
Mph1103I ATGCAT 1 cut(s) 267
MspI CCGG 1 cut(s) 350
MunI CAATTG 1 cut(s) 152
MwoI GCNNNNNNNGC 2 cut(s) 108, 369
NdeI CATATG 1 cut(s) 267
NdeII GATC 1 cut(s) 33
NlaIV GGNNCC 1 cut(s) 143
NmeAIII GCCGAG 1 cut(s) 398
NmuCI GTSAC 1 cut(s) 253
NsiI ATGCAT 1 cut(s) 267
PaeR7I CTCGAG 1 cut(s) 83
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 63
PfeI GAWTC 4 cut(s) 39, 71, 220, 284
PflFI GACNNNGTC 1 cut(s) 59
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
PspEI GGTNACC 1 cut(s) 247
PspFI CCCAGC 1 cut(s) 93
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 91
PspXI VCTCGAGB 1 cut(s) 83
PsyI GACNNNGTC 1 cut(s) 59
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
Sau3AI GATC 1 cut(s) 33
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 401
SduI GDGCHC 2 cut(s) 144, 310
SetI ASST 7 cut(s) 99, 135, 147, 189, 237, 303, 385
SfaNI GCATC 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 83
SlaI CTCGAG 1 cut(s) 83
SmlI CTYRAG 2 cut(s) 83, 113
SmoI CTYRAG 2 cut(s) 83, 113
Sse9I AATT 2 cut(s) 152, 320
SsiI CCGC 2 cut(s) 100, 109
SspMI CTAG 1 cut(s) 384
TaaI ACNGT 2 cut(s) 253, 336
TaiI ACGT 1 cut(s) 303
TaqI TCGA 3 cut(s) 54, 84, 339
TasI AATT 2 cut(s) 152, 320
TatI WGTACW 1 cut(s) 159
TfiI GAWTC 4 cut(s) 39, 71, 220, 284
TseFI GTSAC 1 cut(s) 253
Tsp45I GTSAC 1 cut(s) 253
Tth111I GACNNNGTC 1 cut(s) 59
XhoI CTCGAG 1 cut(s) 83
XmiI GTMKAC 1 cut(s) 63
XspI CTAG 1 cut(s) 384
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.