MD11G1010900.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002071
Physical Location & Seq
Reverse (-)
819632 .. 822490
2859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1010900.v1.1.491

Sequence Viewer

Length: 822 bp
ATGGATGTTACAATTTGGAATAATCGAACAGGGTTAAAATTGTCATTAAATTACCCTCGTAACAAAGAAGTGCATTGGATACCAACAACCATCCAGCCCAATCATAGAACAAATTTTTCTTCCTATATTACCCCTCAGTATTCTCCTAAACTTCCATCTCTCTTCCTCCTCTGTTCTTCCCCAAGTCAAACTCTCACCTTTCTTCCTCAGAGCTCTGCAATAATGGCACCAGGCACCGCTCAGATCTCTCTCTACCTTCTCCTCCTAATCCTTTTCTCAAAAACCGATCTAACTCTATCCCTCAGATACCTCAAACCCAATCCTGACGGTCCATCCAACACCTCCGTATCAATCGCAGACGTCCACGACCTCCTCCCCAAGTACGGCCTCCCAAAGGGTCTCTTACCCGACAACGTAGAATCCTACACTCTCGCAGAAGATGGAAGCTTCGAGATCTTCCTCGGAAGCCCGTGTTACGTGCACTTTGATCAGTTGGTTTACTACAACAAGCATATTAAAGGGAAGCTGAGTTATGGGGCAGTTTCTGATGTGTCTGGGATTCAAGCCAAGAAGCTTTTTATTTGGGTTTCTGTTACCGGGATTCACCGTGACAAAGGATCGGATTCCGTTGAGTTTTACGTTGGTGCCTTGTCGGAGAAGCTGCCGGCGAAACAGTTTGAAGATATTCCTGATTGTAAGAACAAGGCTTGCCATGGTGTCGGAAGTGGTTCTGTAGAAAAAGTACAATGCAATCACTGCATGGAAGAAGGAAACCATCTGCAGATCTTTCCCTCGATCTCATGCAGTCATCTGTATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

274

Amino Acids

30.29

Weight (kDa)

7.12

Isoelectric Point (pI)

39.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 121 - 227 2.2e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 363
AccB1I GGYRCC 3 cut(s) 226, 233, 644
AccBSI CCGCTC 1 cut(s) 239
AciI CCGC 1 cut(s) 237
AclWI GGATC 1 cut(s) 625
AcsI RAATTY 1 cut(s) 112
AcyI GRCGYC 1 cut(s) 360
AfaI GTAC 2 cut(s) 383, 744
AfiI CCNNNNNNNGG 2 cut(s) 383, 394
AgsI TTSAA 2 cut(s) 563, 680
AjnI CCWGG 1 cut(s) 229
AluBI AGCT 5 cut(s) 213, 447, 526, 574, 661
AluI AGCT 5 cut(s) 213, 447, 526, 574, 661
Alw21I GWGCWC 2 cut(s) 215, 483
Alw26I GTCTC 1 cut(s) 404
Alw44I GTGCAC 1 cut(s) 479
AlwI GGATC 1 cut(s) 625
AlwNI CAGNNNCTG 1 cut(s) 545
AoxI GGCC 1 cut(s) 385
ApaLI GTGCAC 1 cut(s) 479
ApeKI GCWGC 1 cut(s) 661
ApoI RAATTY 1 cut(s) 112
Asp700I GAANNNNTTC 2 cut(s) 684, 727
AspS9I GGNCC 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 2 cut(s) 187, 596
AvaII GGWCC 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 483
BaeI ACNNNNGTAYC 2 cut(s) 330, 363
BanI GGYRCC 3 cut(s) 226, 233, 644
BanII GRGCYC 1 cut(s) 215
Bbv12I GWGCWC 2 cut(s) 215, 483
BbvI GCAGC 1 cut(s) 648
BccI CCATC 5 cut(s) 98, 163, 340, 434, 783
BceAI ACGGC 1 cut(s) 400
BciT130I CCWGG 1 cut(s) 231
BciVI GTATCC 1 cut(s) 72
BclI TGATCA 1 cut(s) 487
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 1 cut(s) 404
BfmI CTRYAG 2 cut(s) 732, 779
BfuI GTATCC 1 cut(s) 72
BglII AGATCT 3 cut(s) 243, 453, 783
BisI GCNGC 1 cut(s) 662
BlsI GCNGC 1 cut(s) 663
Bme1390I CCNGG 2 cut(s) 231, 598
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 3 cut(s) 228, 235, 646
BmrFI CCNGG 2 cut(s) 231, 598
BpuMI CCSGG 1 cut(s) 598
BsaAI YACGTR 1 cut(s) 478
BsaHI GRCGYC 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 2 cut(s) 460, 712
Bsc4I CCNNNNNNNGG 2 cut(s) 383, 394
Bse118I RCCGGY 1 cut(s) 664
BseBI CCWGG 1 cut(s) 231
BseDI CCNNGG 2 cut(s) 460, 712
BseGI GGATG 3 cut(s) 10, 90, 332
BseLI CCNNNNNNNGG 2 cut(s) 383, 394
BseMII CTCAG 5 cut(s) 149, 221, 254, 316, 518
BseRI GAGGAG 3 cut(s) 158, 251, 362
BseSI GKGCMC 1 cut(s) 483
BseXI GCAGC 1 cut(s) 648
BshFI GGCC 1 cut(s) 387
BshNI GGYRCC 3 cut(s) 226, 233, 644
BsiHKAI GWGCWC 2 cut(s) 215, 483
BsiSI CCGG 2 cut(s) 597, 665
BslI CCNNNNNNNGG 2 cut(s) 383, 394
BsmAI GTCTC 1 cut(s) 404
BsnI GGCC 1 cut(s) 387
Bso31I GGTCTC 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 215, 483
Bsp143I GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
Bsp19I CCATGG 1 cut(s) 712
BspACI CCGC 1 cut(s) 237
BspANI GGCC 1 cut(s) 387
BspCNI CTCAG 5 cut(s) 148, 220, 253, 315, 519
BspLI GGNNCC 3 cut(s) 228, 235, 646
BspMAI CTGCAG 1 cut(s) 783
BspPI GGATC 1 cut(s) 625
BspT107I GGYRCC 3 cut(s) 226, 233, 644
BspTNI GGTCTC 1 cut(s) 404
BsrBI CCGCTC 1 cut(s) 239
BsrFI RCCGGY 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 2 cut(s) 460, 712
BssMI GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
BssNI GRCGYC 1 cut(s) 360
BssT1I CCWWGG 1 cut(s) 712
Bst2UI CCWGG 1 cut(s) 231
Bst4CI ACNGT 3 cut(s) 329, 608, 675
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 360
BstAPI GCANNNNNTGC 1 cut(s) 756
BstBAI YACGTR 1 cut(s) 478
BstC8I GCNNGC 2 cut(s) 666, 709
BstDEI CTNAG 5 cut(s) 135, 207, 240, 302, 527
BstDSI CCRYGG 1 cut(s) 712
BstENI CCTNNNNNAGG 1 cut(s) 392
BstF5I GGATG 3 cut(s) 10, 90, 332
BstKTI GATC 7 cut(s) 246, 289, 456, 490, 620, 786, 798
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
BstMWI GCNNNNNNNGC 2 cut(s) 224, 756
BstNI CCWGG 1 cut(s) 231
BstSCI CCNGG 2 cut(s) 229, 596
BstSFI CTRYAG 2 cut(s) 732, 779
BstSLI GKGCMC 1 cut(s) 483
BstV1I GCAGC 1 cut(s) 648
BstX2I RGATCY 3 cut(s) 243, 453, 783
BstYI RGATCY 3 cut(s) 243, 453, 783
BsuI GTATCC 1 cut(s) 72
BsuRI GGCC 1 cut(s) 387
BtgI CCRYGG 1 cut(s) 712
BtsCI GGATG 3 cut(s) 10, 90, 332
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 1 cut(s) 754
Cac8I GCNNGC 2 cut(s) 666, 709
CaiI CAGNNNCTG 1 cut(s) 545
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 382, 743
CviAII CATG 3 cut(s) 713, 760, 801
CviQI GTAC 2 cut(s) 382, 743
DdeI CTNAG 5 cut(s) 135, 207, 240, 302, 527
DpnI GATC 7 cut(s) 245, 288, 455, 489, 619, 785, 797
DpnII GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 712
Eco24I GRGCYC 1 cut(s) 215
Eco31I GGTCTC 1 cut(s) 404
Eco47I GGWCC 1 cut(s) 329
Eco53kI GAGCTC 1 cut(s) 213
EcoICRI GAGCTC 1 cut(s) 213
EcoNI CCTNNNNNAGG 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 712
EcoT38I GRGCYC 1 cut(s) 215
ErhI CCWWGG 1 cut(s) 712
FaeI CATG 3 cut(s) 716, 763, 804
FaiI YATR 8 cut(s) 105, 126, 513, 534, 714, 761, 802, 816
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FatI CATG 3 cut(s) 712, 759, 800
FbaI TGATCA 1 cut(s) 487
Fnu4HI GCNGC 1 cut(s) 662
FokI GGATG 3 cut(s) 17, 77, 319
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 1 cut(s) 662
GluI GCNGC 1 cut(s) 662
HaeIII GGCC 1 cut(s) 387
HapII CCGG 2 cut(s) 597, 665
Hin1I GRCGYC 1 cut(s) 360
Hin1II CATG 3 cut(s) 716, 763, 804
HindIII AAGCTT 2 cut(s) 445, 572
HinfI GANTC 4 cut(s) 419, 559, 601, 623
HpaII CCGG 2 cut(s) 597, 665
HphI GGTGA 2 cut(s) 187, 596
Hpy166II GTNNAC 3 cut(s) 364, 481, 499
Hpy188I TCNGA 8 cut(s) 210, 243, 305, 464, 547, 622, 655, 722
Hpy188III TCNNGA 3 cut(s) 323, 451, 689
Hpy8I GTNNAC 3 cut(s) 364, 481, 499
HpyAV CCTTC 2 cut(s) 266, 761
HpyCH4III ACNGT 3 cut(s) 329, 608, 675
HpyCH4IV ACGT 4 cut(s) 360, 414, 477, 639
HpyCH4V TGCA 7 cut(s) 73, 218, 481, 750, 759, 781, 804
HpyF10VI GCNNNNNNNGC 2 cut(s) 224, 756
HpyF3I CTNAG 5 cut(s) 135, 207, 240, 302, 527
HpySE526I ACGT 4 cut(s) 360, 414, 477, 639
Hsp92I GRCGYC 1 cut(s) 360
Hsp92II CATG 3 cut(s) 716, 763, 804
KroI GCCGGC 1 cut(s) 664
KroNI GCCGGC 1 cut(s) 666
Ksp22I TGATCA 1 cut(s) 487
Kzo9I GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
LpnPI CCDG 9 cut(s) 15, 107, 216, 243, 336, 540, 610, 678, 702
Lsp1109I GCAGC 1 cut(s) 648
MaeII ACGT 4 cut(s) 360, 414, 477, 639
MaeIII GTNAC 5 cut(s) 7, 59, 473, 592, 608
MalI GATC 7 cut(s) 245, 288, 455, 489, 619, 785, 797
MbiI CCGCTC 1 cut(s) 239
MboI GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
MboII GAAGA 8 cut(s) 111, 154, 168, 194, 448, 449, 692, 776
MflI RGATCY 3 cut(s) 243, 453, 783
MhlI GDGCHC 2 cut(s) 215, 483
MluCI AATT 4 cut(s) 12, 38, 49, 112
MmeI TCCRAC 3 cut(s) 360, 633, 700
MroNI GCCGGC 1 cut(s) 664
MroXI GAANNNNTTC 2 cut(s) 684, 727
MseI TTAA 3 cut(s) 35, 47, 516
MslI CAYNNNNRTG 1 cut(s) 813
MspI CCGG 2 cut(s) 597, 665
MspR9I CCNGG 2 cut(s) 231, 598
MvaI CCWGG 1 cut(s) 231
MwoI GCNNNNNNNGC 2 cut(s) 224, 756
NaeI GCCGGC 1 cut(s) 666
NciI CCSGG 1 cut(s) 598
NcoI CCATGG 1 cut(s) 712
NdeII GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
NgoMIV GCCGGC 1 cut(s) 664
NlaIII CATG 3 cut(s) 716, 763, 804
NlaIV GGNNCC 3 cut(s) 228, 235, 646
NmuCI GTSAC 1 cut(s) 608
PdiI GCCGGC 1 cut(s) 666
PdmI GAANNNNTTC 2 cut(s) 684, 727
PfeI GAWTC 4 cut(s) 419, 559, 601, 623
PkrI GCNGC 1 cut(s) 663
Ppu21I YACGTR 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 215
Psp6I CCWGG 1 cut(s) 229
PspGI CCWGG 1 cut(s) 229
PspN4I GGNNCC 3 cut(s) 228, 235, 646
PspPI GGNCC 1 cut(s) 329
PstI CTGCAG 1 cut(s) 783
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 3 cut(s) 243, 453, 783
RsaI GTAC 2 cut(s) 383, 744
RsaNI GTAC 2 cut(s) 382, 743
RseI CAYNNNNRTG 1 cut(s) 813
SacI GAGCTC 1 cut(s) 215
SaqAI TTAA 3 cut(s) 35, 47, 516
SatI GCNGC 1 cut(s) 662
Sau3AI GATC 7 cut(s) 243, 286, 453, 487, 617, 783, 795
Sau96I GGNCC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 231, 598
SduI GDGCHC 2 cut(s) 215, 483
SfcI CTRYAG 2 cut(s) 732, 779
SinI GGWCC 1 cut(s) 329
SmiMI CAYNNNNRTG 1 cut(s) 813
Sse9I AATT 4 cut(s) 12, 38, 49, 112
SsiI CCGC 1 cut(s) 237
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 2 cut(s) 229, 596
StyI CCWWGG 1 cut(s) 712
TaaI ACNGT 3 cut(s) 329, 608, 675
TaiI ACGT 4 cut(s) 363, 417, 480, 642
TaqI TCGA 3 cut(s) 25, 450, 794
TasI AATT 4 cut(s) 12, 38, 49, 112
TatI WGTACW 1 cut(s) 742
TfiI GAWTC 4 cut(s) 419, 559, 601, 623
Tru1I TTAA 3 cut(s) 35, 47, 516
Tru9I TTAA 3 cut(s) 35, 47, 516
TscAI CASTG 1 cut(s) 761
TseFI GTSAC 1 cut(s) 608
TseI GCWGC 1 cut(s) 661
Tsp45I GTSAC 1 cut(s) 608
TspGWI ACGGA 2 cut(s) 334, 616
TspRI CASTG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 479
VpaK11BI GGWCC 1 cut(s) 329
XagI CCTNNNNNAGG 1 cut(s) 392
XapI RAATTY 1 cut(s) 112
XmnI GAANNNNTTC 2 cut(s) 684, 727
ZraI GACGTC 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.