MD03G1007700.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002071
Physical Location & Seq
Reverse (-)
614038 .. 616315
2278 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1007700.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGCATCAGCCTCCGCTCAGATCTCTCTCTACCTTCTCCTCCTAACCCTTTTTTCAAAAACCCATCTCACTCTATCCCTCAGAGACCTCAAACCCAATCCCAACGGTTCATCCAACACCTCCCAATCAATTGCAGACGTCCATGACCTCCTCCCCCAGTACGGCCTCCCAAAGGGTCTCTTACCAAACAACGTAAAATCCTACACTCTCACAGAAGACGGAAGCTTCGAGATCTTTCTCGAAAGCCCGTGTTACGTGCACTTTGATCAGTTGGTTTACTACAACAAGCATATTAAAGGGAAGCTGAGTTATGGGGAAGTTTCTGATGTGTCTGGGATTCAAGCCAAGAAGCTTTTTATTTGGGTTTCTGTTACCGGGATTCACCGCGATAAAGGATCGGATTCCGTTGAGTTTTACGTTGGTGCCTTGTCGGAGAAGCTGCCGGCGAAACAGTTTGAAGATATTCCTGATTGTAAGAACAAGGCTTGCCATGGGATCAGAAGTGGTTCTGCAGAAAGAGCACAATGCAAATTATGCAATTACTGCATGGAAGAAGGAAACCATCTGCAGATCTTTCCATCGATCTCTTGCAGTCGTCTTTATGAATATACCGGTGGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

208

Amino Acids

22.98

Weight (kDa)

6.64

Isoelectric Point (pI)

37.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 47 - 153 2.1e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
AccB1I GGYRCC 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 17
AccII CGCG 1 cut(s) 387
AciI CCGC 2 cut(s) 15, 385
AclWI GGATC 2 cut(s) 403, 503
AcoI YGGCCR 1 cut(s) 616
AcyI GRCGYC 1 cut(s) 138
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 161, 172
AgeI ACCGGT 1 cut(s) 611
AgsI TTSAA 3 cut(s) 57, 341, 458
AluBI AGCT 4 cut(s) 225, 304, 352, 439
AluI AGCT 4 cut(s) 225, 304, 352, 439
Alw21I GWGCWC 2 cut(s) 261, 523
Alw26I GTCTC 2 cut(s) 78, 182
Alw44I GTGCAC 1 cut(s) 257
AlwI GGATC 2 cut(s) 403, 503
AoxI GGCC 2 cut(s) 163, 616
ApaLI GTGCAC 1 cut(s) 257
ApeKI GCWGC 1 cut(s) 439
ArsI GACNNNNNNTTYG 2 cut(s) 209, 241
AsiGI ACCGGT 1 cut(s) 611
Asp700I GAANNNNTTC 2 cut(s) 462, 505
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 374
BaeGI GKGCMC 1 cut(s) 261
BalI TGGCCA 1 cut(s) 618
BanI GGYRCC 1 cut(s) 422
BbsI GAAGAC 1 cut(s) 222
Bbv12I GWGCWC 2 cut(s) 261, 523
BbvI GCAGC 1 cut(s) 426
BccI CCATC 3 cut(s) 72, 570, 586
BceAI ACGGC 1 cut(s) 178
BclI TGATCA 1 cut(s) 265
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 2 cut(s) 78, 182
BfmI CTRYAG 2 cut(s) 510, 566
BglII AGATCT 3 cut(s) 21, 231, 570
BisI GCNGC 1 cut(s) 440
BlsI GCNGC 1 cut(s) 441
Bme1390I CCNGG 1 cut(s) 376
BmiI GGNNCC 1 cut(s) 424
BmrFI CCNGG 1 cut(s) 376
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 1 cut(s) 14
BmuI ACTGGG 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 376
Bsa29I ATCGAT 1 cut(s) 581
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 138
BsaI GGTCTC 2 cut(s) 78, 182
BsaJI CCNNGG 1 cut(s) 490
BsaWI WCCGGW 1 cut(s) 611
Bsc4I CCNNNNNNNGG 2 cut(s) 161, 172
Bse118I RCCGGY 2 cut(s) 442, 611
Bse1I ACTGG 2 cut(s) 157, 619
BseCI ATCGAT 1 cut(s) 581
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 1 cut(s) 110
BseLI CCNNNNNNNGG 2 cut(s) 161, 172
BseMII CTCAG 3 cut(s) 32, 94, 296
BseNI ACTGG 2 cut(s) 157, 619
BseRI GAGGAG 2 cut(s) 29, 140
BseSI GKGCMC 1 cut(s) 261
BseXI GCAGC 1 cut(s) 426
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 2 cut(s) 165, 618
BshNI GGYRCC 1 cut(s) 422
BshTI ACCGGT 1 cut(s) 611
BshVI ATCGAT 1 cut(s) 581
BsiHKAI GWGCWC 2 cut(s) 261, 523
BsiSI CCGG 3 cut(s) 375, 443, 612
BslI CCNNNNNNNGG 2 cut(s) 161, 172
BsmAI GTCTC 2 cut(s) 78, 182
BsnI GGCC 2 cut(s) 165, 618
Bso31I GGTCTC 2 cut(s) 78, 182
Bsp1286I GDGCHC 2 cut(s) 261, 523
Bsp143I GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 2 cut(s) 15, 385
BspANI GGCC 2 cut(s) 165, 618
BspCNI CTCAG 3 cut(s) 31, 93, 297
BspDI ATCGAT 1 cut(s) 581
BspFNI CGCG 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 424
BspMAI CTGCAG 2 cut(s) 514, 570
BspPI GGATC 2 cut(s) 403, 503
BspT107I GGYRCC 1 cut(s) 422
BspTNI GGTCTC 2 cut(s) 78, 182
BsrBI CCGCTC 1 cut(s) 17
BsrFI RCCGGY 2 cut(s) 442, 611
BsrI ACTGG 2 cut(s) 157, 619
BssAI RCCGGY 2 cut(s) 442, 611
BssECI CCNNGG 1 cut(s) 490
BssMI GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
BssNI GRCGYC 1 cut(s) 138
BssT1I CCWWGG 1 cut(s) 490
Bst4CI ACNGT 2 cut(s) 107, 453
BstACI GRCGYC 1 cut(s) 138
BstAPI GCANNNNNTGC 2 cut(s) 534, 543
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 444, 487
BstDEI CTNAG 3 cut(s) 18, 80, 305
BstDSI CCRYGG 1 cut(s) 490
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 1 cut(s) 110
BstFNI CGCG 1 cut(s) 387
BstKTI GATC 7 cut(s) 24, 234, 268, 398, 498, 573, 585
BstMAI GTCTC 2 cut(s) 78, 182
BstMBI GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
BstMWI GCNNNNNNNGC 3 cut(s) 518, 534, 543
BstSCI CCNGG 1 cut(s) 374
BstSFI CTRYAG 2 cut(s) 510, 566
BstSLI GKGCMC 1 cut(s) 261
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 1 cut(s) 426
BstV2I GAAGAC 1 cut(s) 222
BstX2I RGATCY 3 cut(s) 21, 231, 570
BstYI RGATCY 3 cut(s) 21, 231, 570
Bsu15I ATCGAT 1 cut(s) 581
BsuRI GGCC 2 cut(s) 165, 618
BsuTUI ATCGAT 1 cut(s) 581
BtgI CCRYGG 1 cut(s) 490
BtsCI GGATG 1 cut(s) 110
Cac8I GCNNGC 2 cut(s) 444, 487
Cfr10I RCCGGY 2 cut(s) 442, 611
ClaI ATCGAT 1 cut(s) 581
Csp6I GTAC 1 cut(s) 160
CspAI ACCGGT 1 cut(s) 611
CviAII CATG 3 cut(s) 143, 491, 547
CviQI GTAC 1 cut(s) 160
DdeI CTNAG 3 cut(s) 18, 80, 305
DpnI GATC 7 cut(s) 23, 233, 267, 397, 497, 572, 584
DpnII GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
EaeI YGGCCR 1 cut(s) 616
Eco130I CCWWGG 1 cut(s) 490
Eco31I GGTCTC 2 cut(s) 78, 182
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
FaeI CATG 3 cut(s) 146, 494, 550
FaiI YATR 8 cut(s) 144, 291, 312, 492, 535, 548, 603, 609
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FatI CATG 3 cut(s) 142, 490, 546
FbaI TGATCA 1 cut(s) 265
Fnu4HI GCNGC 1 cut(s) 440
FokI GGATG 1 cut(s) 97
Fsp4HI GCNGC 1 cut(s) 440
GluI GCNGC 1 cut(s) 440
HaeIII GGCC 2 cut(s) 165, 618
HapII CCGG 3 cut(s) 375, 443, 612
Hin1I GRCGYC 1 cut(s) 138
Hin1II CATG 3 cut(s) 146, 494, 550
HindIII AAGCTT 2 cut(s) 223, 350
HinfI GANTC 3 cut(s) 337, 379, 401
HpaII CCGG 3 cut(s) 375, 443, 612
HphI GGTGA 1 cut(s) 374
Hpy166II GTNNAC 2 cut(s) 259, 277
Hpy188I TCNGA 6 cut(s) 21, 83, 325, 400, 433, 500
Hpy188III TCNNGA 3 cut(s) 229, 239, 467
Hpy8I GTNNAC 2 cut(s) 259, 277
HpyAV CCTTC 2 cut(s) 44, 548
HpyCH4III ACNGT 2 cut(s) 107, 453
HpyCH4IV ACGT 4 cut(s) 138, 192, 255, 417
HpyCH4V TGCA 8 cut(s) 134, 259, 512, 528, 537, 546, 568, 591
HpyF10VI GCNNNNNNNGC 3 cut(s) 518, 534, 543
HpyF3I CTNAG 3 cut(s) 18, 80, 305
HpySE526I ACGT 4 cut(s) 138, 192, 255, 417
Hsp92I GRCGYC 1 cut(s) 138
Hsp92II CATG 3 cut(s) 146, 494, 550
KroI GCCGGC 1 cut(s) 442
KroNI GCCGGC 1 cut(s) 444
Ksp22I TGATCA 1 cut(s) 265
Kzo9I GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
LpnPI CCDG 5 cut(s) 170, 318, 388, 456, 480
Lsp1109I GCAGC 1 cut(s) 426
LweI GCATC 1 cut(s) 14
MaeII ACGT 4 cut(s) 138, 192, 255, 417
MaeIII GTNAC 2 cut(s) 251, 370
MalI GATC 7 cut(s) 23, 233, 267, 397, 497, 572, 584
MbiI CCGCTC 1 cut(s) 17
MboI GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
MboII GAAGA 3 cut(s) 227, 470, 563
MfeI CAATTG 1 cut(s) 129
MflI RGATCY 3 cut(s) 21, 231, 570
MhlI GDGCHC 2 cut(s) 261, 523
MlsI TGGCCA 1 cut(s) 618
MluCI AATT 3 cut(s) 129, 530, 538
MluNI TGGCCA 1 cut(s) 618
MmeI TCCRAC 2 cut(s) 138, 411
MnlI CCTC 8 cut(s) 22, 50, 89, 98, 130, 158, 161, 176
Mox20I TGGCCA 1 cut(s) 618
MroNI GCCGGC 1 cut(s) 442
MroXI GAANNNNTTC 2 cut(s) 462, 505
MscI TGGCCA 1 cut(s) 618
MseI TTAA 1 cut(s) 294
Msp20I TGGCCA 1 cut(s) 618
MspI CCGG 3 cut(s) 375, 443, 612
MspR9I CCNGG 1 cut(s) 376
MunI CAATTG 1 cut(s) 129
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 3 cut(s) 518, 534, 543
NaeI GCCGGC 1 cut(s) 444
NciI CCSGG 1 cut(s) 376
NcoI CCATGG 1 cut(s) 490
NdeII GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
NgoMIV GCCGGC 1 cut(s) 442
NlaIII CATG 3 cut(s) 146, 494, 550
NlaIV GGNNCC 1 cut(s) 424
PcsI WCGNNNNNNNCGW 1 cut(s) 225
PdiI GCCGGC 1 cut(s) 444
PdmI GAANNNNTTC 2 cut(s) 462, 505
PfeI GAWTC 3 cut(s) 337, 379, 401
PinAI ACCGGT 1 cut(s) 611
PkrI GCNGC 1 cut(s) 441
Ppu21I YACGTR 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 424
PstI CTGCAG 2 cut(s) 514, 570
PsuI RGATCY 3 cut(s) 21, 231, 570
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 440
Sau3AI GATC 7 cut(s) 21, 231, 265, 395, 495, 570, 582
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 2 cut(s) 261, 523
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 2 cut(s) 510, 566
Sse9I AATT 3 cut(s) 129, 530, 538
SsiI CCGC 2 cut(s) 15, 385
StyD4I CCNGG 1 cut(s) 374
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 2 cut(s) 107, 453
TaiI ACGT 4 cut(s) 141, 195, 258, 420
TaqI TCGA 3 cut(s) 228, 240, 581
TasI AATT 3 cut(s) 129, 530, 538
TfiI GAWTC 3 cut(s) 337, 379, 401
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TseI GCWGC 1 cut(s) 439
TspDTI ATGAA 2 cut(s) 99, 618
TspGWI ACGGA 2 cut(s) 234, 394
VneI GTGCAC 1 cut(s) 257
XagI CCTNNNNNAGG 1 cut(s) 170
XmnI GAANNNNTTC 2 cut(s) 462, 505
ZraI GACGTC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.