RchiOBHm_Chr5g0082531

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002071
Physical Location & Seq
Forward (+)
88511088 .. 88513410
2323 bp
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UTR
Exon/CDS
Intron
PRQ35673

Sequence Viewer

Length: 510 bp
ATGGCCTCATTTGGCTCTCTCTTCCTTCTAGTCCTAACCCTCTTCTCAGAAACCCAATTATCTCAATCCCTCAGAGACCTCAAACCCAACCCCAACCGTCCATCCACCTCGCTCCAATCAATCACAGACGTCCATGACCTCCTCCCCAAGTACGGCCTCCCAAGGGGTCTGTTACCCGACAACGTAAGATCCTACACTCTATCTGACGATGGAACCTTTGAGATCCATCTCGAAAACCCATGTTATGTTCACTTTGATCAGTTGGTGTACTACAACAAGAACATCAAAGGGAAGCTGAGTTTCGGCTCTGTTTCCGATGTGTCGGGGATTCAAGCCAAGAAGCTATTTATTTGGGTTTCCGTGACGGGGATGCATATGGAACAAGGGTCTGATTCAGTTGAGTTTTACGTTGGGGCTCTGTCCGAGAAATTGCCTGCTAAACAGTTCGAGGATATTCCTGTATGTAAGAGCAAGGCTTGCCGAGAAGGAGCTAGTGTGGAGTCACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

169

Amino Acids

18.77

Weight (kDa)

5.78

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 44 - 150 8.8e-32 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 132
AclWI GGATC 2 cut(s) 183, 217
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 2 cut(s) 152, 269
AfiI CCNNNNNNNGG 3 cut(s) 152, 163, 366
AgsI TTSAA 1 cut(s) 332
AluBI AGCT 3 cut(s) 295, 343, 491
AluI AGCT 3 cut(s) 295, 343, 491
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 2 cut(s) 183, 217
AoxI GGCC 2 cut(s) 3, 154
BanII GRGCYC 1 cut(s) 418
BccI CCATC 3 cut(s) 109, 203, 234
BceAI ACGGC 1 cut(s) 169
BclI TGATCA 1 cut(s) 256
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 2 cut(s) 29, 492
BmiI GGNNCC 1 cut(s) 214
BmsI GCATC 1 cut(s) 360
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 1 cut(s) 161
BsaXI ACNNNNNCTCC 3 cut(s) 480, 491, 510
Bsc4I CCNNNNNNNGG 3 cut(s) 152, 163, 366
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 2 cut(s) 101, 375
BseLI CCNNNNNNNGG 3 cut(s) 152, 163, 366
BseMII CTCAG 3 cut(s) 60, 85, 287
BseRI GAGGAG 1 cut(s) 131
BshFI GGCC 2 cut(s) 5, 156
BslI CCNNNNNNNGG 3 cut(s) 152, 163, 366
BsmAI GTCTC 1 cut(s) 69
BsnI GGCC 2 cut(s) 5, 156
Bso31I GGTCTC 1 cut(s) 69
Bsp1286I GDGCHC 1 cut(s) 418
Bsp143I GATC 3 cut(s) 188, 222, 256
BspANI GGCC 2 cut(s) 5, 156
BspCNI CTCAG 3 cut(s) 59, 84, 288
BspLI GGNNCC 1 cut(s) 214
BspPI GGATC 2 cut(s) 183, 217
BspTNI GGTCTC 1 cut(s) 69
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 3 cut(s) 188, 222, 256
BssNI GRCGYC 1 cut(s) 129
BssT1I CCWWGG 1 cut(s) 161
Bst4CI ACNGT 3 cut(s) 98, 444, 507
Bst6I CTCTTC 2 cut(s) 26, 47
BstACI GRCGYC 1 cut(s) 129
BstAPI GCANNNNNTGC 1 cut(s) 477
BstC8I GCNNGC 2 cut(s) 435, 478
BstDEI CTNAG 3 cut(s) 46, 71, 296
BstF5I GGATG 2 cut(s) 101, 375
BstKTI GATC 3 cut(s) 191, 225, 259
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 3 cut(s) 188, 222, 256
BstMWI GCNNNNNNNGC 1 cut(s) 477
BstX2I RGATCY 2 cut(s) 188, 222
BstYI RGATCY 2 cut(s) 188, 222
BsuRI GGCC 2 cut(s) 5, 156
BtsCI GGATG 2 cut(s) 101, 375
BtsIMutI CAGTG 1 cut(s) 503
Cac8I GCNNGC 2 cut(s) 435, 478
Csp6I GTAC 2 cut(s) 151, 268
CviAII CATG 2 cut(s) 134, 240
CviQI GTAC 2 cut(s) 151, 268
DdeI CTNAG 3 cut(s) 46, 71, 296
DpnI GATC 3 cut(s) 190, 224, 258
DpnII GATC 3 cut(s) 188, 222, 256
Eam1104I CTCTTC 2 cut(s) 26, 47
EarI CTCTTC 2 cut(s) 26, 47
Eco130I CCWWGG 1 cut(s) 161
Eco24I GRGCYC 1 cut(s) 418
Eco31I GGTCTC 1 cut(s) 69
EcoT14I CCWWGG 1 cut(s) 161
EcoT22I ATGCAT 1 cut(s) 375
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 1 cut(s) 161
FaeI CATG 2 cut(s) 137, 243
FaiI YATR 6 cut(s) 135, 241, 246, 375, 377, 463
FatI CATG 2 cut(s) 133, 239
FauNDI CATATG 1 cut(s) 375
FbaI TGATCA 1 cut(s) 256
FokI GGATG 2 cut(s) 88, 382
FriOI GRGCYC 1 cut(s) 418
FspBI CTAG 2 cut(s) 29, 492
HaeIII GGCC 2 cut(s) 5, 156
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 2 cut(s) 137, 243
HinfI GANTC 3 cut(s) 328, 392, 500
Hpy166II GTNNAC 2 cut(s) 250, 268
Hpy188I TCNGA 6 cut(s) 49, 74, 205, 316, 391, 424
Hpy188III TCNNGA 1 cut(s) 230
Hpy8I GTNNAC 2 cut(s) 250, 268
HpyAV CCTTC 2 cut(s) 35, 479
HpyCH4III ACNGT 3 cut(s) 98, 444, 507
HpyCH4IV ACGT 3 cut(s) 129, 183, 408
HpyCH4V TGCA 1 cut(s) 373
HpyF10VI GCNNNNNNNGC 1 cut(s) 477
HpyF3I CTNAG 3 cut(s) 46, 71, 296
HpySE526I ACGT 3 cut(s) 129, 183, 408
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 2 cut(s) 137, 243
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 3 cut(s) 188, 222, 256
LmnI GCTCC 2 cut(s) 117, 488
LpnPI CCDG 2 cut(s) 447, 471
LweI GCATC 1 cut(s) 360
MaeI CTAG 2 cut(s) 29, 492
MaeII ACGT 3 cut(s) 129, 183, 408
MaeIII GTNAC 3 cut(s) 171, 361, 501
MalI GATC 3 cut(s) 190, 224, 258
MboI GATC 3 cut(s) 188, 222, 256
MboII GAAGA 2 cut(s) 13, 34
MflI RGATCY 2 cut(s) 188, 222
MhlI GDGCHC 1 cut(s) 418
MluCI AATT 2 cut(s) 56, 428
MlyI GAGTC 1 cut(s) 509
MnlI CCTC 9 cut(s) 16, 50, 80, 89, 118, 149, 152, 167, 442
Mph1103I ATGCAT 1 cut(s) 375
MwoI GCNNNNNNNGC 1 cut(s) 477
NdeI CATATG 1 cut(s) 375
NdeII GATC 3 cut(s) 188, 222, 256
NlaIII CATG 2 cut(s) 137, 243
NlaIV GGNNCC 1 cut(s) 214
NmeAIII GCCGAG 1 cut(s) 506
NmuCI GTSAC 2 cut(s) 361, 501
NsiI ATGCAT 1 cut(s) 375
PfeI GAWTC 2 cut(s) 328, 392
PleI GAGTC 1 cut(s) 508
PpsI GAGTC 1 cut(s) 508
PspN4I GGNNCC 1 cut(s) 214
PsuI RGATCY 2 cut(s) 188, 222
RsaI GTAC 2 cut(s) 152, 269
RsaNI GTAC 2 cut(s) 151, 268
Sau3AI GATC 3 cut(s) 188, 222, 256
SchI GAGTC 1 cut(s) 509
SduI GDGCHC 1 cut(s) 418
SfaNI GCATC 1 cut(s) 360
Sse9I AATT 2 cut(s) 56, 428
SspMI CTAG 2 cut(s) 29, 492
StyI CCWWGG 1 cut(s) 161
TaaI ACNGT 3 cut(s) 98, 444, 507
TaiI ACGT 3 cut(s) 132, 186, 411
TaqI TCGA 2 cut(s) 231, 447
TasI AATT 2 cut(s) 56, 428
TatI WGTACW 1 cut(s) 267
TfiI GAWTC 2 cut(s) 328, 392
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 2 cut(s) 361, 501
Tsp45I GTSAC 2 cut(s) 361, 501
TspGWI ACGGA 1 cut(s) 349
TspRI CASTG 1 cut(s) 510
XspI CTAG 2 cut(s) 29, 492
ZraI GACGTC 1 cut(s) 130
Zsp2I ATGCAT 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.