AT2G13450

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005646
Physical Location & Seq
Reverse (-)
5598421 .. 5599923
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G13450.1

Sequence Viewer

Length: 1185 bp
ATGGCAGACGAGTTGTGGGATGAGTTACAACACTTGGAGTTGGGAAGGGAGGATCCTGCTCTCTTTATTCCGCATGAAGCCTATGCCATTGTGGAGTCTAGGAATCGGCTTAGTCTGATAGCTAGACCTCTTAATCCTCGCTCTCAGAACCTTCACGCTGTGATCTCGGCTTTACCAAGAGCATGGGGTCTGACTAATAGAGTGCATGGCCGGGTCCTTAATGACACGTTTGTCCAGTTCATCTTTCAATCTGAGATTGATCTCTTGTCAGTACTACGAAGGGAGCCTTGGCTGTATAACAACTGGTTTGTGACAGCTCAGAGATGGGAGGTAAATCTTACGTTTCACCTGTTAACATCTATTGAATTATGGGTTCAGATGCGTGGAATTCCTCTTTTGTATGTGTGTGAGGAAACTGCTTTAGAGATAGCACATGAGTTAGGAGAGATCATCACTCTGGATTTTCATGACTCCACTACGACTCAAATAGCTTATATCCGTGTGAGAATCCGGTTTGGAATTACAGATCGCCTGAGGTTTTTCCTAAGAATCATTTTTGATTCTGGTGAGACTGCTCTGATTAGCTTTCAGTATGAAAGACTTAGGAGAATCTGTAGTAGCTGCTTCAGAATGACTCATCACAGGAACTCATGTCTTTACAGGCAGATTGAGTCTCTCCATAGGGTGACTAACACTACAGCTCAAAGGAATGTTAGAGAAGAGGTGTTCATGAGAGATGAAAATCTGAGATCTTCAATGAATTCACAATCTCAAATGTCTGAGAGTTCGTTTCCTACTCCTATTGATCCACCTCCAAGGATTCCTCATCCTCCTTTAAATCCTGATGAATTGGTGGCTGCATATTACCCTCATACAAGAGCTACCTCTTTACCAAACTTTGCAGGTCCTTTGCCTCAGGTACCTCTTAGAAGAAATGTGGATGAAAGGGATTCTAACATCCAGCCTTTCTCCGGACCTGCATTTGCAGCACACTCTCCACGTCTGGTGGAAGTAGGTGAATCATCAAGGCAAGGTGAGAATACTCAGAATGTTCACACGGTGGAAAAGGGGGATTCATCAAAAAGAAAGAACATGGGTGGTCCCAAGTTTAAAGATGATGCAAGAAAGTCCAATGAAGATGAGCATATGAATGGAGGGATTCTGAAACCTCCAAAAAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

394

Amino Acids

45.55

Weight (kDa)

7.28

Isoelectric Point (pI)

50.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 33 - 112 1.3e-18 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 172 - 220 4.3e-20 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 230
Acc36I ACCTGC 2 cut(s) 893, 985
Acc65I GGTACC 1 cut(s) 919
AccB1I GGYRCC 1 cut(s) 919
AccIII TCCGGA 1 cut(s) 971
AciI CCGC 1 cut(s) 71
AclWI GGATC 3 cut(s) 47, 60, 800
AcoI YGGCCR 1 cut(s) 208
AcsI RAATTY 2 cut(s) 387, 760
AcuI CTGAAG 1 cut(s) 610
AdeI CACNNNGTG 2 cut(s) 160, 1060
AfaI GTAC 2 cut(s) 273, 921
AfiI CCNNNNNNNGG 1 cut(s) 971
AflIII ACRYGT 1 cut(s) 225
AgsI TTSAA 3 cut(s) 248, 365, 756
AjiI CACGTC 1 cut(s) 1001
AjuI GAANNNNNNNTTGG 2 cut(s) 271, 303
AluBI AGCT 7 cut(s) 122, 317, 491, 585, 621, 701, 881
AluI AGCT 7 cut(s) 122, 317, 491, 585, 621, 701, 881
Alw26I GTCTC 2 cut(s) 563, 678
AlwI GGATC 3 cut(s) 47, 60, 800
Aor13HI TCCGGA 1 cut(s) 971
AoxI GGCC 1 cut(s) 208
ApeKI GCWGC 3 cut(s) 621, 857, 986
ApoI RAATTY 2 cut(s) 387, 760
Asp718I GGTACC 1 cut(s) 919
AspS9I GGNCC 4 cut(s) 214, 905, 974, 1100
AsuC2I CCSGG 1 cut(s) 212
AsuHPI GGTGA 5 cut(s) 338, 578, 697, 1028, 1046
AvaII GGWCC 4 cut(s) 214, 905, 974, 1100
AxyI CCTNAGG 2 cut(s) 533, 915
BamHI GGATCC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 919
BbvI GCAGC 3 cut(s) 608, 844, 998
BccI CCATC 1 cut(s) 318
BcnI CCSGG 1 cut(s) 212
BcoDI GTCTC 2 cut(s) 563, 678
BfaI CTAG 2 cut(s) 99, 123
BfmI CTRYAG 2 cut(s) 613, 696
BfuAI ACCTGC 2 cut(s) 893, 985
BglII AGATCT 1 cut(s) 749
BisI GCNGC 3 cut(s) 622, 858, 987
BlsI GCNGC 3 cut(s) 623, 859, 988
BmcAI AGTACT 1 cut(s) 273
Bme1390I CCNGG 1 cut(s) 212
Bme18I GGWCC 4 cut(s) 214, 905, 974, 1100
BmgBI CACGTC 1 cut(s) 1001
BmgT120I GGNCC 4 cut(s) 214, 905, 974, 1100
BmiI GGNNCC 5 cut(s) 54, 215, 285, 921, 1102
BmrFI CCNGG 1 cut(s) 212
BmsI GCATC 2 cut(s) 369, 1108
BpuMI CCSGG 1 cut(s) 212
BsaBI GATNNNNATC 1 cut(s) 741
BsaJI CCNNGG 2 cut(s) 287, 815
BsaWI WCCGGW 2 cut(s) 510, 971
BsaXI ACNNNNNCTCC 2 cut(s) 598, 628
Bsc4I CCNNNNNNNGG 1 cut(s) 971
Bse1I ACTGG 2 cut(s) 235, 308
Bse21I CCTNAGG 2 cut(s) 533, 915
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 971
BseDI CCNNGG 2 cut(s) 287, 815
BseGI GGATG 4 cut(s) 25, 826, 946, 957
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 1 cut(s) 971
BseMII CTCAG 8 cut(s) 158, 243, 332, 524, 737, 771, 929, 1058
BseNI ACTGG 2 cut(s) 235, 308
BseXI GCAGC 3 cut(s) 608, 844, 998
BshFI GGCC 1 cut(s) 210
BshNI GGYRCC 1 cut(s) 919
BsiSI CCGG 3 cut(s) 211, 511, 972
BslFI GGGAC 1 cut(s) 1086
BslI CCNNNNNNNGG 1 cut(s) 971
BsmAI GTCTC 2 cut(s) 563, 678
BsmFI GGGAC 1 cut(s) 1086
BsnI GGCC 1 cut(s) 210
Bsp13I TCCGGA 1 cut(s) 971
Bsp143I GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
BspACI CCGC 1 cut(s) 71
BspANI GGCC 1 cut(s) 210
BspCNI CTCAG 8 cut(s) 157, 244, 331, 525, 738, 772, 928, 1057
BspEI TCCGGA 1 cut(s) 971
BspHI TCATGA 2 cut(s) 466, 729
BspLI GGNNCC 5 cut(s) 54, 215, 285, 921, 1102
BspMI ACCTGC 2 cut(s) 893, 985
BspPI GGATC 3 cut(s) 47, 60, 800
BspT107I GGYRCC 1 cut(s) 919
BsrI ACTGG 2 cut(s) 235, 308
BssECI CCNNGG 2 cut(s) 287, 815
BssMI GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
BssT1I CCWWGG 2 cut(s) 287, 815
Bst4CI ACNGT 1 cut(s) 1060
Bst6I CTCTTC 1 cut(s) 714
BstF5I GGATG 4 cut(s) 25, 826, 946, 957
BstKTI GATC 7 cut(s) 55, 165, 262, 450, 529, 752, 808
BstMAI GTCTC 2 cut(s) 563, 678
BstMBI GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
BstMWI GCNNNNNNNGC 1 cut(s) 986
BstSCI CCNGG 1 cut(s) 210
BstSFI CTRYAG 2 cut(s) 613, 696
BstV1I GCAGC 3 cut(s) 608, 844, 998
BstX2I RGATCY 2 cut(s) 52, 749
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 2 cut(s) 52, 749
Bsu36I CCTNAGG 2 cut(s) 533, 915
BsuRI GGCC 1 cut(s) 210
BtrI CACGTC 1 cut(s) 1001
BtsCI GGATG 4 cut(s) 25, 826, 946, 957
BveI ACCTGC 2 cut(s) 893, 985
CciI TCATGA 2 cut(s) 466, 729
Cfr13I GGNCC 4 cut(s) 214, 905, 974, 1100
Csp6I GTAC 2 cut(s) 272, 920
CviAII CATG 8 cut(s) 74, 183, 206, 434, 467, 651, 730, 1093
CviQI GTAC 2 cut(s) 272, 920
DpnI GATC 7 cut(s) 54, 164, 261, 449, 528, 751, 807
DpnII GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
DraI TTTAAA 2 cut(s) 837, 1111
DraIII CACNNNGTG 2 cut(s) 160, 1060
DrdI GACNNNNNNGTC 1 cut(s) 230
DseDI GACNNNNNNGTC 1 cut(s) 230
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 1 cut(s) 714
EarI CTCTTC 1 cut(s) 714
Eco130I CCWWGG 2 cut(s) 287, 815
Eco47I GGWCC 4 cut(s) 214, 905, 974, 1100
Eco57I CTGAAG 1 cut(s) 610
Eco81I CCTNAGG 2 cut(s) 533, 915
EcoO109I RGGNCCY 2 cut(s) 214, 905
EcoRI GAATTC 2 cut(s) 387, 760
EcoT14I CCWWGG 2 cut(s) 287, 815
ErhI CCWWGG 2 cut(s) 287, 815
FaeI CATG 8 cut(s) 77, 186, 209, 437, 470, 654, 733, 1096
FalI AAGNNNNNCTT 2 cut(s) 271, 303
FaqI GGGAC 1 cut(s) 1086
FatI CATG 8 cut(s) 73, 182, 205, 433, 466, 650, 729, 1092
FauNDI CATATG 1 cut(s) 1146
Fnu4HI GCNGC 3 cut(s) 622, 858, 987
FokI GGATG 4 cut(s) 32, 813, 944, 953
Fsp4HI GCNGC 3 cut(s) 622, 858, 987
FspBI CTAG 2 cut(s) 99, 123
GluI GCNGC 3 cut(s) 622, 858, 987
HaeIII GGCC 1 cut(s) 210
HapII CCGG 3 cut(s) 211, 511, 972
Hin1II CATG 8 cut(s) 77, 186, 209, 437, 470, 654, 733, 1096
HincII GTYRAC 1 cut(s) 354
HindII GTYRAC 1 cut(s) 354
HpaI GTTAAC 1 cut(s) 354
HpaII CCGG 3 cut(s) 211, 511, 972
HphI GGTGA 5 cut(s) 338, 578, 697, 1028, 1046
Hpy166II GTNNAC 2 cut(s) 354, 1054
Hpy188III TCNNGA 5 cut(s) 458, 467, 730, 842, 972
Hpy8I GTNNAC 2 cut(s) 354, 1054
HpyAV CCTTC 3 cut(s) 39, 161, 273
HpyCH4III ACNGT 1 cut(s) 1060
HpyCH4IV ACGT 3 cut(s) 227, 341, 1000
HpyCH4V TGCA 6 cut(s) 205, 860, 902, 980, 986, 1121
HpyF10VI GCNNNNNNNGC 1 cut(s) 986
HpySE526I ACGT 3 cut(s) 227, 341, 1000
Hsp92II CATG 8 cut(s) 77, 186, 209, 437, 470, 654, 733, 1096
Kpn2I TCCGGA 1 cut(s) 971
KpnI GGTACC 1 cut(s) 923
KspAI GTTAAC 1 cut(s) 354
Kzo9I GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
LmnI GCTCC 1 cut(s) 283
Lsp1109I GCAGC 3 cut(s) 608, 844, 998
LweI GCATC 2 cut(s) 369, 1108
MaeI CTAG 2 cut(s) 99, 123
MaeII ACGT 3 cut(s) 227, 341, 1000
MaeIII GTNAC 3 cut(s) 24, 310, 685
MalI GATC 7 cut(s) 54, 164, 261, 449, 528, 751, 807
MboI GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
MboII GAAGA 4 cut(s) 731, 744, 942, 1148
MflI RGATCY 2 cut(s) 52, 749
MluCI AATT 5 cut(s) 365, 387, 519, 760, 848
MlyI GAGTC 5 cut(s) 104, 464, 475, 628, 680
MroI TCCGGA 1 cut(s) 971
MseI TTAA 5 cut(s) 132, 219, 353, 836, 1110
MslI CAYNNNNRTG 1 cut(s) 1149
MspI CCGG 3 cut(s) 211, 511, 972
MspR9I CCNGG 1 cut(s) 212
MwoI GCNNNNNNNGC 1 cut(s) 986
NciI CCSGG 1 cut(s) 212
NdeI CATATG 1 cut(s) 1146
NdeII GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
NlaIII CATG 8 cut(s) 77, 186, 209, 437, 470, 654, 733, 1096
NlaIV GGNNCC 5 cut(s) 54, 215, 285, 921, 1102
NmeAIII GCCGAG 1 cut(s) 146
NmuCI GTSAC 2 cut(s) 310, 685
PagI TCATGA 2 cut(s) 466, 729
PkrI GCNGC 3 cut(s) 623, 859, 988
PleI GAGTC 5 cut(s) 103, 464, 475, 628, 679
PpsI GAGTC 5 cut(s) 103, 464, 475, 628, 679
PpuMI RGGWCCY 2 cut(s) 214, 905
Psp5II RGGWCCY 2 cut(s) 214, 905
PspN4I GGNNCC 5 cut(s) 54, 215, 285, 921, 1102
PspPI GGNCC 4 cut(s) 214, 905, 974, 1100
PspPPI RGGWCCY 2 cut(s) 214, 905
PsuI RGATCY 2 cut(s) 52, 749
RsaI GTAC 2 cut(s) 273, 921
RsaNI GTAC 2 cut(s) 272, 920
RseI CAYNNNNRTG 1 cut(s) 1149
SaqAI TTAA 5 cut(s) 132, 219, 353, 836, 1110
SatI GCNGC 3 cut(s) 622, 858, 987
Sau3AI GATC 7 cut(s) 52, 162, 259, 447, 526, 749, 805
Sau96I GGNCC 4 cut(s) 214, 905, 974, 1100
ScaI AGTACT 1 cut(s) 273
SchI GAGTC 5 cut(s) 104, 464, 475, 628, 680
ScrFI CCNGG 1 cut(s) 212
SfaNI GCATC 2 cut(s) 369, 1108
SfcI CTRYAG 2 cut(s) 613, 696
SinI GGWCC 4 cut(s) 214, 905, 974, 1100
SmiMI CAYNNNNRTG 1 cut(s) 1149
Sse9I AATT 5 cut(s) 365, 387, 519, 760, 848
SsiI CCGC 1 cut(s) 71
SspMI CTAG 2 cut(s) 99, 123
StyD4I CCNGG 1 cut(s) 210
StyI CCWWGG 2 cut(s) 287, 815
TaaI ACNGT 1 cut(s) 1060
TaiI ACGT 3 cut(s) 230, 344, 1003
TasI AATT 5 cut(s) 365, 387, 519, 760, 848
TatI WGTACW 1 cut(s) 271
Tru1I TTAA 5 cut(s) 132, 219, 353, 836, 1110
Tru9I TTAA 5 cut(s) 132, 219, 353, 836, 1110
TseFI GTSAC 2 cut(s) 310, 685
TseI GCWGC 3 cut(s) 621, 857, 986
Tsp45I GTSAC 2 cut(s) 310, 685
TspGWI ACGGA 1 cut(s) 488
VpaK11BI GGWCC 4 cut(s) 214, 905, 974, 1100
XapI RAATTY 2 cut(s) 387, 760
XspI CTAG 2 cut(s) 99, 123
ZrmI AGTACT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.