AT5G35737

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005646
Physical Location & Seq
Forward (+)
13907164 .. 13907535
372 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G35737.1

Sequence Viewer

Length: 372 bp
ATGGCGGATGAGTTATGGGATGAGTTACAACATTTAGAGTTTGGAAGGGAGGATCCAGCTCTGTTTATTGCACATGCAGCTTATGCAACTGTTGAATCTCGGAATAGGCTTAGCTTGATTGCTAGGCCTCTTAGTCCTCGTTCTCAAAACCGCAACGCTGTAATTGCTATTTTACCAAAATCATGGGGTCTGACTTCTAGAGTTTATGGAAGAGTGCTTAATATAACTTTTGTTCAATTTCTCTCTCAGTCTGAGGTTGATCATTTGTATGTGCTAAAAAGGGAACCGTGGTTATATAACAGTTGGTTTGTCACGGCTCAGAGATGGGAGGTAAATCTTGCTTTTGAGTTCTTGTCAAATATTGACTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

123

Amino Acids

14.38

Weight (kDa)

5.88

Isoelectric Point (pI)

57.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 33 - 113 2.1e-14 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 151
AclWI GGATC 2 cut(s) 47, 60
AgsI TTSAA 2 cut(s) 95, 236
AluBI AGCT 3 cut(s) 59, 80, 114
AluI AGCT 3 cut(s) 59, 80, 114
AlwI GGATC 2 cut(s) 47, 60
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 1 cut(s) 77
BamHI GGATCC 1 cut(s) 52
BbvI GCAGC 1 cut(s) 89
BccI CCATC 1 cut(s) 318
BceAI ACGGC 1 cut(s) 330
BclI TGATCA 1 cut(s) 259
BfaI CTAG 2 cut(s) 123, 198
BisI GCNGC 1 cut(s) 78
BlpI GCTNAGC 1 cut(s) 110
BlsI GCNGC 1 cut(s) 79
BmiI GGNNCC 2 cut(s) 54, 285
Bpu1102I GCTNAGC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 287
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 2 cut(s) 13, 25
BseMII CTCAG 3 cut(s) 243, 260, 332
BseXI GCAGC 1 cut(s) 89
BshFI GGCC 1 cut(s) 127
BsnI GGCC 1 cut(s) 127
Bsp143I GATC 2 cut(s) 52, 259
Bsp1720I GCTNAGC 1 cut(s) 110
BspACI CCGC 2 cut(s) 5, 151
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 3 cut(s) 244, 259, 331
BspLI GGNNCC 2 cut(s) 54, 285
BspPI GGATC 2 cut(s) 47, 60
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 2 cut(s) 52, 259
Bst4CI ACNGT 3 cut(s) 91, 288, 302
Bst6I CTCTTC 1 cut(s) 205
BstAPI GCANNNNNTGC 1 cut(s) 83
BstDEI CTNAG 5 cut(s) 110, 131, 246, 252, 318
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 2 cut(s) 13, 25
BstKTI GATC 2 cut(s) 55, 262
BstMBI GATC 2 cut(s) 52, 259
BstMWI GCNNNNNNNGC 3 cut(s) 77, 83, 164
BstNSI RCATGY 1 cut(s) 77
BstV1I GCAGC 1 cut(s) 89
BstX2I RGATCY 1 cut(s) 52
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 1 cut(s) 52
BsuRI GGCC 1 cut(s) 127
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 2 cut(s) 13, 25
CviAII CATG 2 cut(s) 74, 183
CviJI RGCY 6 cut(s) 59, 80, 109, 114, 127, 317
CviKI_1 RGCY 6 cut(s) 59, 80, 109, 114, 127, 317
DdeI CTNAG 5 cut(s) 110, 131, 246, 252, 318
DpnI GATC 2 cut(s) 54, 261
DpnII GATC 2 cut(s) 52, 259
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 1 cut(s) 20
Eco147I AGGCCT 1 cut(s) 127
FaeI CATG 2 cut(s) 77, 186
FaiI YATR 9 cut(s) 16, 75, 84, 184, 207, 224, 270, 295, 297
FatI CATG 2 cut(s) 73, 182
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 1 cut(s) 78
FokI GGATG 2 cut(s) 20, 32
Fsp4HI GCNGC 1 cut(s) 78
FspBI CTAG 2 cut(s) 123, 198
GluI GCNGC 1 cut(s) 78
HaeIII GGCC 1 cut(s) 127
Hin1II CATG 2 cut(s) 77, 186
HinfI GANTC 1 cut(s) 95
Hpy188I TCNGA 4 cut(s) 102, 192, 253, 321
Hpy188III TCNNGA 1 cut(s) 198
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 3 cut(s) 91, 288, 302
HpyCH4V TGCA 3 cut(s) 71, 77, 86
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 83, 164
HpyF3I CTNAG 5 cut(s) 110, 131, 246, 252, 318
Hsp92II CATG 2 cut(s) 77, 186
Ksp22I TGATCA 1 cut(s) 259
Kzo9I GATC 2 cut(s) 52, 259
LpnPI CCDG 1 cut(s) 69
Lsp1109I GCAGC 1 cut(s) 89
MaeI CTAG 2 cut(s) 123, 198
MaeIII GTNAC 2 cut(s) 24, 310
MalI GATC 2 cut(s) 54, 261
MboI GATC 2 cut(s) 52, 259
MboII GAAGA 1 cut(s) 222
MflI RGATCY 1 cut(s) 52
MluCI AATT 2 cut(s) 162, 236
MnlI CCTC 5 cut(s) 43, 138, 147, 247, 322
MseI TTAA 1 cut(s) 219
MslI CAYNNNNRTG 1 cut(s) 267
MwoI GCNNNNNNNGC 3 cut(s) 77, 83, 164
NdeII GATC 2 cut(s) 52, 259
NlaIII CATG 2 cut(s) 77, 186
NlaIV GGNNCC 2 cut(s) 54, 285
NmuCI GTSAC 1 cut(s) 310
NspI RCATGY 1 cut(s) 77
PceI AGGCCT 1 cut(s) 127
PfeI GAWTC 1 cut(s) 95
PkrI GCNGC 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 54, 285
PsuI RGATCY 1 cut(s) 52
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 1 cut(s) 219
SatI GCNGC 1 cut(s) 78
Sau3AI GATC 2 cut(s) 52, 259
SetI ASST 5 cut(s) 61, 82, 116, 258, 333
SmiMI CAYNNNNRTG 1 cut(s) 267
Sse9I AATT 2 cut(s) 162, 236
SseBI AGGCCT 1 cut(s) 127
SsiI CCGC 2 cut(s) 5, 151
SspI AATATT 1 cut(s) 361
SspMI CTAG 2 cut(s) 123, 198
StuI AGGCCT 1 cut(s) 127
TaaI ACNGT 3 cut(s) 91, 288, 302
TasI AATT 2 cut(s) 162, 236
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 219
Tru9I TTAA 1 cut(s) 219
TseFI GTSAC 1 cut(s) 310
TseI GCWGC 1 cut(s) 77
Tsp45I GTSAC 1 cut(s) 310
XbaI TCTAGA 1 cut(s) 197
XceI RCATGY 1 cut(s) 77
XspI CTAG 2 cut(s) 123, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.