AT5G36228

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005646
Physical Location & Seq
Forward (+)
14271349 .. 14273258
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G36228.1

Sequence Viewer

Length: 1086 bp
ATGAGTGATGAATTGTGGAACGCCATACAACATATGGATTTAGGTCGTGAGGAGCCTGAACTTTATATCCCATATCATGCTTATGTTGGAGCGTTGGCTTCTAACAGATTAAGTCTCCTTGGAAGAATTCTAAATCCTCAAACTCAAAGCGTTGAAAGAGCTATCTTGGAGCTACCTTATCAGTGGGGACTTGGAACTCAGGTACATGGACGCATCCTTGACGACAGATGTTTTCAAGTTCGTTTTCGCTCGGAGATAGATCTACTTAATGGGCTAAGAAGAGCGCCTTGGGTTTTTAATGAATGGTTTATTGCACTTCAGAGATGGGAAGACTTTCCTACAGAGGATTTCCTTACCTTTATAGATGTTTGGGTTCATATTAGAGGAATTCCTTTACCATATGTGTCAGAAAGGACAGTTGAGATCATTGCTAGTACTTTAGGAGAGGTTGTGGCTATGGATTTCAATGAAGAAACTACATCGCAGATAACCTTTATTCGTGTTAAGGTTAGAATGGATTTCACTGAGCCACTACGTTTCTTTCGAAGAGTACGGTTTGCATCTAGAGAAAGAGCCATGATTGGCTTTGAGTATGAGAAGCTTCAGCGAGTTTGCACCAACTGCTGTCGAGTGAATCATCAAGTCAGTCATTGCCCGTATGTTGTGCATCAGGAGGAGATGGATAATGAACCAGATGTTTTGGTCTCTCCAGAGAGGTATGATGATGAAGACAGTCTCAATCAAGAAGATCACGGGAGACACTCACAAAGTTCTGTTATCTCATCGTTTTCATCCTTAACTCCAATATCACTGAATGCGCCTCCTGTTGTAAACTGGAATGATAACATGATAGGCAACATACCTCATAGATTCCCATCAACTTCAGTCTCATCTTCTCACACTGTTTCAGATGGTTACCTTGCAGCTTCGGAATGGAGACCTAAAGATCAAGTTAGCTATGAGGTTGGTGAGAGTTCAAAGAGAAAGAAAGGGAAGCAAGTATTAGAAGTGCCTGAACGCAGCATCCGACAGAGACGAATGGGATCCGGGATAAGGTTTTACCCGGTGAATGGTGAAAATCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

361

Amino Acids

41.88

Weight (kDa)

5.63

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 34 - 112 5.1e-18 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 172 - 220 5.7e-24 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 1038, 1051
AcsI RAATTY 2 cut(s) 126, 387
AcuI CTGAAG 3 cut(s) 302, 587, 867
AfaI GTAC 3 cut(s) 204, 436, 552
AfiI CCNNNNNNNGG 2 cut(s) 1053, 1070
AgsI TTSAA 4 cut(s) 155, 236, 466, 978
AjuI GAANNNNNNNTTGG 2 cut(s) 271, 303
AloI GAACNNNNNNTCC 4 cut(s) 51, 83, 1008, 1040
AluBI AGCT 5 cut(s) 161, 172, 601, 926, 957
AluI AGCT 5 cut(s) 161, 172, 601, 926, 957
Alw26I GTCTC 7 cut(s) 119, 709, 740, 751, 892, 931, 1027
AlwI GGATC 2 cut(s) 1038, 1051
ApeKI GCWGC 2 cut(s) 923, 1020
ApoI RAATTY 2 cut(s) 126, 387
Asp700I GAANNNNTTC 1 cut(s) 333
AspLEI GCGC 2 cut(s) 286, 820
AsuC2I CCSGG 2 cut(s) 1048, 1064
AsuHPI GGTGA 3 cut(s) 980, 1078, 1085
AsuII TTCGAA 1 cut(s) 544
BamHI GGATCC 1 cut(s) 1043
BbsI GAAGAC 2 cut(s) 336, 735
BbvI GCAGC 2 cut(s) 935, 1032
BccI CCATC 4 cut(s) 318, 673, 883, 905
BcnI CCSGG 2 cut(s) 1048, 1064
BcoDI GTCTC 7 cut(s) 119, 709, 740, 751, 892, 931, 1027
BfaI CTAG 2 cut(s) 432, 564
BfmI CTRYAG 1 cut(s) 339
BfoI RGCGCY 1 cut(s) 287
BglII AGATCT 1 cut(s) 259
BisI GCNGC 2 cut(s) 924, 1021
BlsI GCNGC 2 cut(s) 925, 1022
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 2 cut(s) 1048, 1064
BmiI GGNNCC 2 cut(s) 54, 1045
BmrFI CCNGG 2 cut(s) 1048, 1064
BmsI GCATC 4 cut(s) 222, 569, 676, 1032
BpiI GAAGAC 2 cut(s) 336, 735
BpmI CTGGAG 1 cut(s) 693
Bpu14I TTCGAA 1 cut(s) 544
BpuMI CCSGG 2 cut(s) 1048, 1064
BsaBI GATNNNNATC 1 cut(s) 874
BsaI GGTCTC 2 cut(s) 709, 931
BsaJI CCNNGG 2 cut(s) 118, 287
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 1053, 1070
Bse1I ACTGG 1 cut(s) 839
Bse3DI GCAATG 2 cut(s) 426, 649
Bse8I GATNNNNATC 1 cut(s) 874
BseDI CCNNGG 2 cut(s) 118, 287
BseGI GGATG 3 cut(s) 213, 791, 1023
BseJI GATNNNNATC 1 cut(s) 874
BseLI CCNNNNNNNGG 2 cut(s) 1053, 1070
BseMI GCAATG 2 cut(s) 426, 649
BseMII CTCAG 2 cut(s) 212, 516
BseNI ACTGG 1 cut(s) 839
BseRI GAGGAG 2 cut(s) 65, 689
BseXI GCAGC 2 cut(s) 935, 1032
BsiSI CCGG 2 cut(s) 1047, 1064
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 1053, 1070
BsmAI GTCTC 7 cut(s) 119, 709, 740, 751, 892, 931, 1027
BsmBI CGTCTC 1 cut(s) 1027
BsmFI GGGAC 1 cut(s) 201
BsmI GAATGC 1 cut(s) 820
Bso31I GGTCTC 2 cut(s) 709, 931
Bsp119I TTCGAA 1 cut(s) 544
Bsp143I GATC 5 cut(s) 259, 423, 748, 946, 1043
BspCNI CTCAG 2 cut(s) 211, 517
BspLI GGNNCC 2 cut(s) 54, 1045
BspPI GGATC 2 cut(s) 1038, 1051
BspQI GCTCTTC 1 cut(s) 274
BspT104I TTCGAA 1 cut(s) 544
BspTNI GGTCTC 2 cut(s) 709, 931
BsrDI GCAATG 2 cut(s) 426, 649
BsrI ACTGG 1 cut(s) 839
BssECI CCNNGG 2 cut(s) 118, 287
BssMI GATC 5 cut(s) 259, 423, 748, 946, 1043
BssT1I CCWWGG 2 cut(s) 118, 287
Bst4CI ACNGT 4 cut(s) 418, 555, 734, 904
Bst6I CTCTTC 2 cut(s) 274, 541
BstAPI GCANNNNNTGC 1 cut(s) 621
BstBI TTCGAA 1 cut(s) 544
BstDEI CTNAG 3 cut(s) 198, 275, 525
BstEII GGTNACC 1 cut(s) 914
BstF5I GGATG 3 cut(s) 213, 791, 1023
BstH2I RGCGCY 1 cut(s) 287
BstHHI GCGC 2 cut(s) 286, 820
BstKTI GATC 5 cut(s) 262, 426, 751, 949, 1046
BstMAI GTCTC 7 cut(s) 119, 709, 740, 751, 892, 931, 1027
BstMBI GATC 5 cut(s) 259, 423, 748, 946, 1043
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstPI GGTNACC 1 cut(s) 914
BstSCI CCNGG 2 cut(s) 1046, 1062
BstSFI CTRYAG 1 cut(s) 339
BstV1I GCAGC 2 cut(s) 935, 1032
BstV2I GAAGAC 2 cut(s) 336, 735
BstX2I RGATCY 2 cut(s) 259, 1043
BstYI RGATCY 2 cut(s) 259, 1043
BtgZI GCGATG 1 cut(s) 465
BtsCI GGATG 3 cut(s) 213, 791, 1023
BtsIMutI CAGTG 4 cut(s) 188, 522, 809, 900
CfoI GCGC 2 cut(s) 286, 820
CseI GACGC 1 cut(s) 219
Csp6I GTAC 3 cut(s) 203, 435, 551
CviAII CATG 4 cut(s) 77, 206, 577, 847
CviQI GTAC 3 cut(s) 203, 435, 551
DdeI CTNAG 3 cut(s) 198, 275, 525
DpnI GATC 5 cut(s) 261, 425, 750, 948, 1045
DpnII GATC 5 cut(s) 259, 423, 748, 946, 1043
Eam1104I CTCTTC 2 cut(s) 274, 541
EarI CTCTTC 2 cut(s) 274, 541
Eco130I CCWWGG 2 cut(s) 118, 287
Eco31I GGTCTC 2 cut(s) 709, 931
Eco57I CTGAAG 3 cut(s) 302, 587, 867
Eco91I GGTNACC 1 cut(s) 914
EcoO65I GGTNACC 1 cut(s) 914
EcoRI GAATTC 2 cut(s) 126, 387
EcoT14I CCWWGG 2 cut(s) 118, 287
ErhI CCWWGG 2 cut(s) 118, 287
Esp3I CGTCTC 1 cut(s) 1027
FaeI CATG 4 cut(s) 80, 209, 580, 850
FalI AAGNNNNNCTT 2 cut(s) 271, 303
FaqI GGGAC 1 cut(s) 201
FatI CATG 4 cut(s) 76, 205, 576, 846
FauNDI CATATG 2 cut(s) 33, 400
Fnu4HI GCNGC 2 cut(s) 924, 1021
FokI GGATG 3 cut(s) 200, 778, 1010
Fsp4HI GCNGC 2 cut(s) 924, 1021
FspBI CTAG 2 cut(s) 432, 564
GlaI GCGC 2 cut(s) 285, 819
GluI GCNGC 2 cut(s) 924, 1021
GsuI CTGGAG 1 cut(s) 693
HaeII RGCGCY 1 cut(s) 287
HapII CCGG 2 cut(s) 1047, 1064
HgaI GACGC 1 cut(s) 219
HhaI GCGC 2 cut(s) 286, 820
Hin1II CATG 4 cut(s) 80, 209, 580, 850
Hin6I GCGC 2 cut(s) 284, 818
HinP1I GCGC 2 cut(s) 284, 818
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 2 cut(s) 634, 870
HpaII CCGG 2 cut(s) 1047, 1064
HphI GGTGA 3 cut(s) 980, 1078, 1085
Hpy166II GTNNAC 1 cut(s) 832
Hpy188I TCNGA 6 cut(s) 253, 321, 409, 910, 931, 1028
Hpy188III TCNNGA 5 cut(s) 47, 564, 671, 710, 743
Hpy8I GTNNAC 1 cut(s) 832
HpyCH4III ACNGT 4 cut(s) 418, 555, 734, 904
HpyCH4IV ACGT 1 cut(s) 535
HpyCH4V TGCA 5 cut(s) 314, 560, 615, 667, 923
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpyF3I CTNAG 3 cut(s) 198, 275, 525
HpySE526I ACGT 1 cut(s) 535
Hsp92II CATG 4 cut(s) 80, 209, 580, 850
HspAI GCGC 2 cut(s) 284, 818
Kzo9I GATC 5 cut(s) 259, 423, 748, 946, 1043
LguI GCTCTTC 1 cut(s) 274
LmnI GCTCC 3 cut(s) 52, 89, 169
Lsp1109I GCAGC 2 cut(s) 935, 1032
LweI GCATC 4 cut(s) 222, 569, 676, 1032
MaeI CTAG 2 cut(s) 432, 564
MaeII ACGT 1 cut(s) 535
MaeIII GTNAC 1 cut(s) 914
MalI GATC 5 cut(s) 261, 425, 750, 948, 1045
MboI GATC 5 cut(s) 259, 423, 748, 946, 1043
MboII GAAGA 8 cut(s) 135, 291, 341, 482, 558, 740, 758, 885
MflI RGATCY 2 cut(s) 259, 1043
MluCI AATT 3 cut(s) 11, 126, 387
MmeI TCCRAC 2 cut(s) 67, 1051
MroXI GAANNNNTTC 1 cut(s) 333
MseI TTAA 6 cut(s) 110, 267, 297, 504, 797, 1084
MslI CAYNNNNRTG 1 cut(s) 81
MspI CCGG 2 cut(s) 1047, 1064
MspR9I CCNGG 2 cut(s) 1048, 1064
Mva1269I GAATGC 1 cut(s) 820
MwoI GCNNNNNNNGC 1 cut(s) 621
NciI CCSGG 2 cut(s) 1048, 1064
NdeI CATATG 2 cut(s) 33, 400
NdeII GATC 5 cut(s) 259, 423, 748, 946, 1043
NlaIII CATG 4 cut(s) 80, 209, 580, 850
NlaIV GGNNCC 2 cut(s) 54, 1045
NspV TTCGAA 1 cut(s) 544
PciSI GCTCTTC 1 cut(s) 274
PcsI WCGNNNNNNNCGW 2 cut(s) 541, 1024
PctI GAATGC 1 cut(s) 820
PdmI GAANNNNTTC 1 cut(s) 333
PfeI GAWTC 2 cut(s) 634, 870
PfoI TCCNGGA 1 cut(s) 1046
PkrI GCNGC 2 cut(s) 925, 1022
PspEI GGTNACC 1 cut(s) 914
PspN4I GGNNCC 2 cut(s) 54, 1045
PsuI RGATCY 2 cut(s) 259, 1043
RsaI GTAC 3 cut(s) 204, 436, 552
RsaNI GTAC 3 cut(s) 203, 435, 551
RseI CAYNNNNRTG 1 cut(s) 81
SapI GCTCTTC 1 cut(s) 274
SaqAI TTAA 6 cut(s) 110, 267, 297, 504, 797, 1084
SatI GCNGC 2 cut(s) 924, 1021
Sau3AI GATC 5 cut(s) 259, 423, 748, 946, 1043
ScaI AGTACT 1 cut(s) 436
ScrFI CCNGG 2 cut(s) 1048, 1064
SfaNI GCATC 4 cut(s) 222, 569, 676, 1032
SfcI CTRYAG 1 cut(s) 339
SfuI TTCGAA 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 81
Sse9I AATT 3 cut(s) 11, 126, 387
SspMI CTAG 2 cut(s) 432, 564
StyD4I CCNGG 2 cut(s) 1046, 1062
StyI CCWWGG 2 cut(s) 118, 287
TaaI ACNGT 4 cut(s) 418, 555, 734, 904
TaiI ACGT 1 cut(s) 538
TaqI TCGA 2 cut(s) 544, 628
TasI AATT 3 cut(s) 11, 126, 387
TatI WGTACW 1 cut(s) 434
TfiI GAWTC 2 cut(s) 634, 870
Tru1I TTAA 6 cut(s) 110, 267, 297, 504, 797, 1084
Tru9I TTAA 6 cut(s) 110, 267, 297, 504, 797, 1084
TscAI CASTG 4 cut(s) 188, 529, 816, 907
TseI GCWGC 2 cut(s) 923, 1020
TspDTI ATGAA 7 cut(s) 24, 315, 365, 483, 702, 741, 780
TspRI CASTG 4 cut(s) 188, 529, 816, 907
XapI RAATTY 2 cut(s) 126, 387
XbaI TCTAGA 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 31
XmnI GAANNNNTTC 1 cut(s) 333
XspI CTAG 2 cut(s) 432, 564
ZrmI AGTACT 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.