AT5G51195

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005646
Physical Location & Seq
Reverse (-)
20803106 .. 20804268
1163 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51195.1

Sequence Viewer

Length: 804 bp
ATGGTTCCTCTTCCTGCACCAAGAAATGATGGTAGAGGTCAAGACATGGCAGAAAGGACAAGAGCGGCTTTACATGAGGAGCTTTGCAGGTCGGATATGAATTCTCAATCACAACTGTCAGATGACTCCTTTCCTGCTCCTATAACTCCACCTCCAAGGACTGCTACCCCTCCTTTGAATTCTGCAGAGCTTGCAGCTGCAAGACTTTACCTGTTCCCTTCAAGAACAGCTACTCCTCAGAACTTGATGGTTGAAAAACCTCAAATGGCAACTCTTACACCACGTTTCAGGTCAGAATCTCATCTCTCTCCACCAATTGCAGATGAAGTCACTTCTAGATTTTCAAGAATTTTAGAGATTGGTGAATCATCAAGAAGAGCTGAGATTGGGGAAGGTTCCAGGCCAACTGAAGAAGGTGACTCATCTAAACGCAAGACCACACAAGTTGAACAGCCAGAGGCAGTTCGAAGGAATCAAAAGCAGAAAGTAACAGAGCTTAAAGAAGGAGGGATTCTGAAACCTCCAAAGAAGCGCAAGGGTTTGAATAATTGGTATGCTCAGGATTTTATTGGAGTATTTGCATACCTAATGGTTAGTGTGCAGCAATTCCGTTTGAATAGACTGGAGATTCAAGCAGAAATGGAATCTGAAGATGTAGCTGCAACCTTTTGGATCGATGATGAAACTCACGATGACTTTTTTGCGTCTAGCAAGTCTGGATGGTGGTGTGATCGTGGTGATTTGGTTGTTGGGTTTAGGGATTTTTTTTACTGGGCAGCTATTTTAGGTTATTTGGAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

267

Amino Acids

30.24

Weight (kDa)

5.61

Isoelectric Point (pI)

58.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 78
AccBSI CCGCTC 1 cut(s) 65
AciI CCGC 1 cut(s) 65
AclWI GGATC 1 cut(s) 680
AcsI RAATTY 3 cut(s) 100, 178, 348
AcuI CTGAAG 2 cut(s) 429, 669
AgsI TTSAA 8 cut(s) 178, 222, 254, 345, 449, 544, 616, 632
AjnI CCWGG 1 cut(s) 398
AloI GAACNNNNNNTCC 2 cut(s) 217, 249
AluBI AGCT 8 cut(s) 82, 190, 197, 230, 380, 496, 659, 779
AluI AGCT 8 cut(s) 82, 190, 197, 230, 380, 496, 659, 779
AlwI GGATC 1 cut(s) 680
AoxI GGCC 1 cut(s) 401
ApeKI GCWGC 5 cut(s) 194, 197, 601, 659, 776
ApoI RAATTY 3 cut(s) 100, 178, 348
AspLEI GCGC 1 cut(s) 534
AsuHPI GGTGA 3 cut(s) 374, 428, 749
AsuII TTCGAA 1 cut(s) 466
BbvI GCAGC 5 cut(s) 184, 206, 613, 646, 788
BccI CCATC 3 cut(s) 23, 241, 714
BciT130I CCWGG 1 cut(s) 400
BfaI CTAG 2 cut(s) 336, 708
BfmI CTRYAG 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 78
BisI GCNGC 6 cut(s) 66, 195, 198, 602, 660, 777
BlsI GCNGC 6 cut(s) 67, 196, 199, 603, 661, 778
Bme1390I CCNGG 1 cut(s) 400
BmiI GGNNCC 2 cut(s) 6, 397
BmrFI CCNGG 1 cut(s) 400
BmrI ACTGGG 1 cut(s) 781
BmuI ACTGGG 1 cut(s) 781
BpmI CTGGAG 1 cut(s) 644
Bpu10I CCTNAGC 1 cut(s) 558
Bpu14I TTCGAA 1 cut(s) 466
Bsa29I ATCGAT 1 cut(s) 675
BsaJI CCNNGG 1 cut(s) 155
BsaXI ACNNNNNCTCC 4 cut(s) 136, 166, 217, 247
Bse1I ACTGG 2 cut(s) 627, 776
BseBI CCWGG 1 cut(s) 400
BseCI ATCGAT 1 cut(s) 675
BseDI CCNNGG 1 cut(s) 155
BseGI GGATG 1 cut(s) 725
BseMII CTCAG 3 cut(s) 251, 372, 572
BseNI ACTGG 2 cut(s) 627, 776
BseRI GAGGAG 2 cut(s) 92, 225
BseXI GCAGC 5 cut(s) 184, 206, 613, 646, 788
BsgI GTGCAG 1 cut(s) 620
BshFI GGCC 1 cut(s) 403
BshVI ATCGAT 1 cut(s) 675
BsnI GGCC 1 cut(s) 403
Bsp119I TTCGAA 1 cut(s) 466
Bsp143I GATC 2 cut(s) 672, 730
BspACI CCGC 1 cut(s) 65
BspANI GGCC 1 cut(s) 403
BspCNI CTCAG 3 cut(s) 250, 373, 571
BspDI ATCGAT 1 cut(s) 675
BspLI GGNNCC 2 cut(s) 6, 397
BspMAI CTGCAG 1 cut(s) 187
BspMI ACCTGC 1 cut(s) 78
BspPI GGATC 1 cut(s) 680
BspQI GCTCTTC 1 cut(s) 370
BspT104I TTCGAA 1 cut(s) 466
BsrBI CCGCTC 1 cut(s) 65
BsrI ACTGG 2 cut(s) 627, 776
BssECI CCNNGG 1 cut(s) 155
BssMI GATC 2 cut(s) 672, 730
BssT1I CCWWGG 1 cut(s) 155
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 1 cut(s) 117
Bst6I CTCTTC 2 cut(s) 15, 370
BstAPI GCANNNNNTGC 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 466
BstC8I GCNNGC 1 cut(s) 192
BstDEI CTNAG 3 cut(s) 237, 381, 558
BstF5I GGATG 1 cut(s) 725
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 2 cut(s) 675, 733
BstMBI GATC 2 cut(s) 672, 730
BstMWI GCNNNNNNNGC 1 cut(s) 191
BstNI CCWGG 1 cut(s) 400
BstSCI CCNGG 1 cut(s) 398
BstSFI CTRYAG 1 cut(s) 183
BstV1I GCAGC 5 cut(s) 184, 206, 613, 646, 788
Bsu15I ATCGAT 1 cut(s) 675
BsuRI GGCC 1 cut(s) 403
BsuTUI ATCGAT 1 cut(s) 675
BtsCI GGATG 1 cut(s) 725
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 1 cut(s) 192
CfoI GCGC 1 cut(s) 534
ClaI ATCGAT 1 cut(s) 675
CseI GACGC 1 cut(s) 693
CspCI CAANNNNNGTGG 2 cut(s) 427, 462
CviAII CATG 2 cut(s) 46, 74
DdeI CTNAG 3 cut(s) 237, 381, 558
DpnI GATC 2 cut(s) 674, 732
DpnII GATC 2 cut(s) 672, 730
Eam1104I CTCTTC 2 cut(s) 15, 370
EarI CTCTTC 2 cut(s) 15, 370
Eco130I CCWWGG 1 cut(s) 155
Eco57I CTGAAG 2 cut(s) 429, 669
EcoRI GAATTC 2 cut(s) 100, 178
EcoRII CCWGG 1 cut(s) 398
EcoT14I CCWWGG 1 cut(s) 155
ErhI CCWWGG 1 cut(s) 155
FaeI CATG 2 cut(s) 49, 77
FaiI YATR 6 cut(s) 47, 75, 98, 143, 555, 583
FalI AAGNNNNNCTT 2 cut(s) 52, 84
FatI CATG 2 cut(s) 45, 73
Fnu4HI GCNGC 6 cut(s) 66, 195, 198, 602, 660, 777
FokI GGATG 1 cut(s) 732
Fsp4HI GCNGC 6 cut(s) 66, 195, 198, 602, 660, 777
FspBI CTAG 2 cut(s) 336, 708
GlaI GCGC 1 cut(s) 533
GluI GCNGC 6 cut(s) 66, 195, 198, 602, 660, 777
GsuI CTGGAG 1 cut(s) 644
HaeIII GGCC 1 cut(s) 403
HgaI GACGC 1 cut(s) 693
HhaI GCGC 1 cut(s) 534
Hin1II CATG 2 cut(s) 49, 77
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HinfI GANTC 8 cut(s) 125, 296, 365, 419, 472, 511, 628, 644
HphI GGTGA 3 cut(s) 374, 428, 749
Hpy188I TCNGA 6 cut(s) 94, 121, 240, 295, 516, 649
Hpy188III TCNNGA 8 cut(s) 41, 222, 336, 345, 372, 560, 689, 717
HpyAV CCTTC 5 cut(s) 228, 386, 407, 462, 497
HpyCH4III ACNGT 1 cut(s) 117
HpyCH4IV ACGT 1 cut(s) 283
HpyCH4V TGCA 9 cut(s) 17, 87, 185, 194, 200, 320, 581, 601, 662
HpyF10VI GCNNNNNNNGC 1 cut(s) 191
HpyF3I CTNAG 3 cut(s) 237, 381, 558
HpySE526I ACGT 1 cut(s) 283
Hsp92II CATG 2 cut(s) 49, 77
HspAI GCGC 1 cut(s) 532
Kzo9I GATC 2 cut(s) 672, 730
LguI GCTCTTC 1 cut(s) 370
LmnI GCTCC 2 cut(s) 79, 142
Lsp1109I GCAGC 5 cut(s) 184, 206, 613, 646, 788
MaeI CTAG 2 cut(s) 336, 708
MaeII ACGT 1 cut(s) 283
MaeIII GTNAC 3 cut(s) 328, 416, 487
MalI GATC 2 cut(s) 674, 732
MbiI CCGCTC 1 cut(s) 65
MboI GATC 2 cut(s) 672, 730
MboII GAAGA 3 cut(s) 387, 422, 662
MfeI CAATTG 1 cut(s) 315
MluCI AATT 6 cut(s) 100, 178, 315, 348, 547, 605
MlyI GAGTC 2 cut(s) 119, 413
MmeI TCCRAC 1 cut(s) 72
MseI TTAA 1 cut(s) 498
MspA1I CMGCKG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 400
MunI CAATTG 1 cut(s) 315
MvaI CCWGG 1 cut(s) 400
MwoI GCNNNNNNNGC 1 cut(s) 191
NdeII GATC 2 cut(s) 672, 730
NlaIII CATG 2 cut(s) 49, 77
NlaIV GGNNCC 2 cut(s) 6, 397
NmuCI GTSAC 2 cut(s) 328, 416
NspV TTCGAA 1 cut(s) 466
PciSI GCTCTTC 1 cut(s) 370
PfeI GAWTC 6 cut(s) 296, 365, 472, 511, 628, 644
PkrI GCNGC 6 cut(s) 67, 196, 199, 603, 661, 778
PleI GAGTC 2 cut(s) 119, 413
PpsI GAGTC 2 cut(s) 119, 413
Psp6I CCWGG 1 cut(s) 398
PspGI CCWGG 1 cut(s) 398
PspN4I GGNNCC 2 cut(s) 6, 397
PstI CTGCAG 1 cut(s) 187
PvuII CAGCTG 1 cut(s) 197
SapI GCTCTTC 1 cut(s) 370
SaqAI TTAA 1 cut(s) 498
SatI GCNGC 6 cut(s) 66, 195, 198, 602, 660, 777
Sau3AI GATC 2 cut(s) 672, 730
SchI GAGTC 2 cut(s) 119, 413
ScrFI CCNGG 1 cut(s) 400
SfcI CTRYAG 1 cut(s) 183
SfuI TTCGAA 1 cut(s) 466
Sse9I AATT 6 cut(s) 100, 178, 315, 348, 547, 605
SsiI CCGC 1 cut(s) 65
SspMI CTAG 2 cut(s) 336, 708
StyD4I CCNGG 1 cut(s) 398
StyI CCWWGG 1 cut(s) 155
TaaI ACNGT 1 cut(s) 117
TaiI ACGT 1 cut(s) 286
TaqI TCGA 2 cut(s) 466, 675
TasI AATT 6 cut(s) 100, 178, 315, 348, 547, 605
TauI GCSGC 1 cut(s) 68
TfiI GAWTC 6 cut(s) 296, 365, 472, 511, 628, 644
Tru1I TTAA 1 cut(s) 498
Tru9I TTAA 1 cut(s) 498
TseFI GTSAC 2 cut(s) 328, 416
TseI GCWGC 5 cut(s) 194, 197, 601, 659, 776
Tsp45I GTSAC 2 cut(s) 328, 416
TspDTI ATGAA 3 cut(s) 113, 339, 696
TspGWI ACGGA 1 cut(s) 599
XapI RAATTY 3 cut(s) 100, 178, 348
XbaI TCTAGA 1 cut(s) 335
XspI CTAG 2 cut(s) 336, 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.