AT5G25600

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005646
Physical Location & Seq
Reverse (-)
8912959 .. 8914055
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25600.1

Sequence Viewer

Length: 996 bp
ATGGCTGATGAGATTTGGCATGATATACAGTACATGGTTTTGGGTCGTGATGATCCAGAACTTTTTGTACCTCAGGCAGCTTATGGTGTTGTTGTTGCTGGCAACAGACTGAGTCTTATTGCAAGACCGCTTAACATCCATGCTCAACACCTCAGAAGAGTACTTAGGGCCTTGCCTCGCAGCTGGGGTATGGCTTCTCGTGTACATGGCCGCATCATTGATGATAGGTGCATCCAGTTTCGTTTTCGCTCTGGTATTGACTTGGCTTCGGTCATGCTGAGAGGCCCATGGTTATTCAACCAATGGTTTGTACCTCTGCAGAGATGGGAAGACTTTCCCCGTGCTGACTTTCTTACCTATATTGATCTTTGGGTTCAAGTTAGAGGCATACCTCTCTATGTGTCTACTATGACTGTCAGGTTTATTGCTAGTACTCTGGGGCCAGTCACGGGGCTTGATTTTGATGAAGAGACCTCTACTCAGATAGCCTTTATCAGAGTGAAAGTGAGGATTTCCATCACGGATAGGATGCGTTTCTTTCGACGGGTGCGGTTTGAATCACGAGAAGGGGATCACCATAATGATGGTGCTCATGTTGTTGATGATGAAGCGGATGTTTTGATGGTTCCTGTTTGGGAAGAGGGAGGGGGATCGAACAATCCTACACCCCCTCCACCTGCGAATCACTCTTCAAGTTCTGATATCTCTTCTCACAGACCTATATCACAGGCACCAACCTCTGCTTCACCGGGTTTAGACTTGAATGAACCTGCTATTGAACATCTTCCTTCAAGTGTTCCATTAGTAAGTCCTAAAGCTCTCTCTGATGATTCCACCACTCCTACTGCTATTAATGATCAATCAAGATATGCATGTGGTGAGAGTTCTAAACGCAGAAAAGGAAAGCAGATTGACCTTACTCCTGATAAAAAGAAGAAACAATATCGCAAAGATTATGGAGTAAGGTTTTATTCGGAGTCTGATAACACACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

331

Amino Acids

37.49

Weight (kDa)

7.17

Isoelectric Point (pI)

53.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 35 - 111 6.1e-15 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 685
Acc36I ACCTGC 2 cut(s) 685, 778
AccB1I GGYRCC 1 cut(s) 730
AccI GTMKAC 1 cut(s) 404
AciI CCGC 4 cut(s) 128, 211, 550, 611
AclWI GGATC 3 cut(s) 47, 579, 658
AcoI YGGCCR 1 cut(s) 208
AfaI GTAC 6 cut(s) 32, 69, 162, 204, 312, 433
AfiI CCNNNNNNNGG 1 cut(s) 449
AgsI TTSAA 7 cut(s) 298, 377, 557, 693, 763, 779, 792
AjuI GAANNNNNNNTTGG 2 cut(s) 727, 759
AluBI AGCT 3 cut(s) 80, 183, 818
AluI AGCT 3 cut(s) 80, 183, 818
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 1 cut(s) 464
AlwI GGATC 3 cut(s) 47, 579, 658
AoxI GGCC 4 cut(s) 168, 208, 283, 440
ApeKI GCWGC 2 cut(s) 77, 180
AseI ATTAAT 1 cut(s) 852
Asp700I GAANNNNTTC 2 cut(s) 333, 783
AspS9I GGNCC 3 cut(s) 168, 284, 440
AsuC2I CCSGG 1 cut(s) 750
AsuHPI GGTGA 3 cut(s) 566, 738, 890
AxyI CCTNAGG 1 cut(s) 72
BanI GGYRCC 1 cut(s) 730
BauI CACGAG 2 cut(s) 198, 561
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 2 cut(s) 89, 192
BccI CCATC 4 cut(s) 318, 524, 578, 616
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 1 cut(s) 750
BcoDI GTCTC 1 cut(s) 464
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 317
BfuAI ACCTGC 2 cut(s) 685, 778
BisI GCNGC 3 cut(s) 78, 181, 211
BlsI GCNGC 3 cut(s) 79, 182, 212
BmcAI AGTACT 2 cut(s) 162, 433
Bme1390I CCNGG 1 cut(s) 750
BmgT120I GGNCC 3 cut(s) 168, 284, 440
BmiI GGNNCC 3 cut(s) 441, 627, 732
BmrFI CCNGG 1 cut(s) 750
BmsI GCATC 3 cut(s) 222, 240, 519
BpiI GAAGAC 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 750
BsaBI GATNNNNATC 1 cut(s) 515
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 655, 685
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 2 cut(s) 235, 443
Bse21I CCTNAGG 1 cut(s) 72
Bse8I GATNNNNATC 1 cut(s) 515
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 4 cut(s) 135, 231, 534, 619
BseJI GATNNNNATC 1 cut(s) 515
BseLI CCNNNNNNNGG 1 cut(s) 449
BseMII CTCAG 5 cut(s) 86, 101, 166, 269, 494
BseNI ACTGG 2 cut(s) 235, 443
BseXI GCAGC 2 cut(s) 89, 192
BseYI CCCAGC 1 cut(s) 183
BshFI GGCC 4 cut(s) 170, 210, 285, 442
BshNI GGYRCC 1 cut(s) 730
BsiHKAI GWGCWC 1 cut(s) 592
BsiSI CCGG 1 cut(s) 749
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 464
BsnI GGCC 4 cut(s) 170, 210, 285, 442
Bso31I GGTCTC 1 cut(s) 464
Bsp1286I GDGCHC 1 cut(s) 592
Bsp1407I TGTACA 1 cut(s) 202
Bsp143I GATC 5 cut(s) 52, 364, 571, 650, 856
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 4 cut(s) 128, 211, 550, 611
BspANI GGCC 4 cut(s) 170, 210, 285, 442
BspCNI CTCAG 5 cut(s) 85, 102, 165, 270, 493
BspLI GGNNCC 3 cut(s) 441, 627, 732
BspMAI CTGCAG 1 cut(s) 321
BspMI ACCTGC 2 cut(s) 685, 778
BspPI GGATC 3 cut(s) 47, 579, 658
BspT107I GGYRCC 1 cut(s) 730
BspTNI GGTCTC 1 cut(s) 464
BsrGI TGTACA 1 cut(s) 202
BsrI ACTGG 2 cut(s) 235, 443
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 5 cut(s) 52, 364, 571, 650, 856
BssSI CACGAG 2 cut(s) 198, 561
BssT1I CCWWGG 1 cut(s) 287
Bst2BI CACGAG 2 cut(s) 198, 561
Bst4CI ACNGT 2 cut(s) 30, 415
Bst6I CTCTTC 5 cut(s) 151, 462, 633, 694, 712
BstAUI TGTACA 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 100
BstDEI CTNAG 6 cut(s) 72, 110, 152, 164, 278, 480
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 4 cut(s) 135, 231, 534, 619
BstKTI GATC 5 cut(s) 55, 367, 574, 653, 859
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 5 cut(s) 52, 364, 571, 650, 856
BstNSI RCATGY 1 cut(s) 876
BstSCI CCNGG 1 cut(s) 748
BstSFI CTRYAG 1 cut(s) 317
BstV1I GCAGC 2 cut(s) 89, 192
BstV2I GAAGAC 1 cut(s) 336
BstXI CCANNNNNNTGG 1 cut(s) 584
Bsu36I CCTNAGG 1 cut(s) 72
BsuRI GGCC 4 cut(s) 170, 210, 285, 442
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 4 cut(s) 135, 231, 534, 619
BveI ACCTGC 2 cut(s) 685, 778
Cac8I GCNNGC 1 cut(s) 100
Cfr13I GGNCC 3 cut(s) 168, 284, 440
Csp6I GTAC 6 cut(s) 31, 68, 161, 203, 311, 432
CviAII CATG 8 cut(s) 20, 34, 140, 206, 274, 288, 593, 873
CviQI GTAC 6 cut(s) 31, 68, 161, 203, 311, 432
DdeI CTNAG 6 cut(s) 72, 110, 152, 164, 278, 480
DpnI GATC 5 cut(s) 54, 366, 573, 652, 858
DpnII GATC 5 cut(s) 52, 364, 571, 650, 856
EaeI YGGCCR 1 cut(s) 208
Eam1104I CTCTTC 5 cut(s) 151, 462, 633, 694, 712
EarI CTCTTC 5 cut(s) 151, 462, 633, 694, 712
Eco130I CCWWGG 1 cut(s) 287
Eco31I GGTCTC 1 cut(s) 464
Eco32I GATATC 1 cut(s) 703
Eco81I CCTNAGG 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 168
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 287
EcoT22I ATGCAT 1 cut(s) 874
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 8 cut(s) 23, 37, 143, 209, 277, 291, 596, 876
FatI CATG 8 cut(s) 19, 33, 139, 205, 273, 287, 592, 872
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 3 cut(s) 78, 181, 211
FokI GGATG 4 cut(s) 122, 218, 541, 626
Fsp4HI GCNGC 3 cut(s) 78, 181, 211
FspBI CTAG 1 cut(s) 429
GluI GCNGC 3 cut(s) 78, 181, 211
GsaI CCCAGC 1 cut(s) 187
HaeIII GGCC 4 cut(s) 170, 210, 285, 442
HapII CCGG 1 cut(s) 749
Hin1II CATG 8 cut(s) 23, 37, 143, 209, 277, 291, 596, 876
HinfI GANTC 5 cut(s) 112, 557, 682, 830, 977
HpaII CCGG 1 cut(s) 749
HphI GGTGA 3 cut(s) 566, 738, 890
Hpy166II GTNNAC 2 cut(s) 203, 405
Hpy188I TCNGA 7 cut(s) 155, 483, 497, 700, 826, 976, 982
Hpy188III TCNNGA 5 cut(s) 47, 56, 561, 864, 923
Hpy8I GTNNAC 2 cut(s) 203, 405
Hpy99I CGWCG 1 cut(s) 546
HpyAV CCTTC 2 cut(s) 560, 798
HpyCH4III ACNGT 2 cut(s) 30, 415
HpyCH4V TGCA 4 cut(s) 122, 231, 319, 872
HpyF3I CTNAG 6 cut(s) 72, 110, 152, 164, 278, 480
Hsp92II CATG 8 cut(s) 23, 37, 143, 209, 277, 291, 596, 876
Ksp22I TGATCA 1 cut(s) 856
Kzo9I GATC 5 cut(s) 52, 364, 571, 650, 856
Lsp1109I GCAGC 2 cut(s) 89, 192
LweI GCATC 3 cut(s) 222, 240, 519
MaeI CTAG 1 cut(s) 429
MaeIII GTNAC 1 cut(s) 445
MalI GATC 5 cut(s) 54, 366, 573, 652, 858
MboI GATC 5 cut(s) 52, 364, 571, 650, 856
MboII GAAGA 8 cut(s) 168, 341, 479, 650, 681, 699, 776, 946
MhlI GDGCHC 1 cut(s) 592
MlyI GAGTC 2 cut(s) 121, 986
Mph1103I ATGCAT 1 cut(s) 874
MroXI GAANNNNTTC 2 cut(s) 333, 783
MseI TTAA 2 cut(s) 132, 852
MslI CAYNNNNRTG 2 cut(s) 579, 582
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 1 cut(s) 749
MspR9I CCNGG 1 cut(s) 750
NciI CCSGG 1 cut(s) 750
NcoI CCATGG 1 cut(s) 287
NdeII GATC 5 cut(s) 52, 364, 571, 650, 856
NlaIII CATG 8 cut(s) 23, 37, 143, 209, 277, 291, 596, 876
NlaIV GGNNCC 3 cut(s) 441, 627, 732
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 1 cut(s) 874
NspI RCATGY 1 cut(s) 876
PaqCI CACCTGC 1 cut(s) 685
PdmI GAANNNNTTC 2 cut(s) 333, 783
PfeI GAWTC 3 cut(s) 557, 682, 830
PflFI GACNNNGTC 1 cut(s) 111
PkrI GCNGC 3 cut(s) 79, 182, 212
PleI GAGTC 2 cut(s) 120, 985
PpsI GAGTC 2 cut(s) 120, 985
PshBI ATTAAT 1 cut(s) 852
PspFI CCCAGC 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 441, 627, 732
PspPI GGNCC 3 cut(s) 168, 284, 440
PsrI GAACNNNNNNTAC 2 cut(s) 51, 83
PstI CTGCAG 1 cut(s) 321
PsyI GACNNNGTC 1 cut(s) 111
PvuII CAGCTG 1 cut(s) 183
RsaI GTAC 6 cut(s) 32, 69, 162, 204, 312, 433
RsaNI GTAC 6 cut(s) 31, 68, 161, 203, 311, 432
RseI CAYNNNNRTG 2 cut(s) 579, 582
SaqAI TTAA 2 cut(s) 132, 852
SatI GCNGC 3 cut(s) 78, 181, 211
Sau3AI GATC 5 cut(s) 52, 364, 571, 650, 856
Sau96I GGNCC 3 cut(s) 168, 284, 440
ScaI AGTACT 2 cut(s) 162, 433
SchI GAGTC 2 cut(s) 121, 986
ScrFI CCNGG 1 cut(s) 750
SduI GDGCHC 1 cut(s) 592
SfaNI GCATC 3 cut(s) 222, 240, 519
SfcI CTRYAG 1 cut(s) 317
SmiMI CAYNNNNRTG 2 cut(s) 579, 582
SsiI CCGC 4 cut(s) 128, 211, 550, 611
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 1 cut(s) 748
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 30, 415
TaqI TCGA 2 cut(s) 541, 653
TaqII GACCGA 1 cut(s) 259
TatI WGTACW 4 cut(s) 30, 160, 202, 431
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 3 cut(s) 557, 682, 830
Tru1I TTAA 2 cut(s) 132, 852
Tru9I TTAA 2 cut(s) 132, 852
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 2 cut(s) 77, 180
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 3 cut(s) 480, 621, 780
TspGWI ACGGA 1 cut(s) 536
Tth111I GACNNNGTC 1 cut(s) 111
VspI ATTAAT 1 cut(s) 852
XceI RCATGY 1 cut(s) 876
XmiI GTMKAC 1 cut(s) 404
XmnI GAANNNNTTC 2 cut(s) 333, 783
XspI CTAG 1 cut(s) 429
ZrmI AGTACT 2 cut(s) 162, 433
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.