AT5G36225

Domain of unknown function (DUF4283)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005646
Physical Location & Seq
Reverse (-)
14263970 .. 14264735
766 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G36225.1

Sequence Viewer

Length: 708 bp
ATGTACCGCGGGTTAAATTTTAGTATCAATAATATAAGGAAACGATCTTGGGTTCGGCTTTGCAATATTCCTGTCAATGATTATACGAAAGAGACGATTAAGGAAATTGCTGCTTGTGTTGGGGAGCTGTTAAAGGTGGAGTTAGATTTGGAGAAATCTCAAGCTCAAGATTATGTGAGAGTTCAAGTGCTTTTTGATGTTCGAAATCCATTGCGTAACTCTAAGGAAGTGCAAGTACCTATAGGTGAAGTGGTTTCAGTTACTTTTGATTATGAGCGTATTCGAAAGAGATGTTTCTTTTGCCAAATATTTACTCATGACAAATTTGACTGTCCCAGCCGTCAACAAGGAAGTCTGCAACTTCCTAGTGCGAGTTTAAAGAAGTGTTTACCTGAGAGAGTGATGGTTGATCAGCTTATTAATCCCTTGGTGGGAATGGATTTTAATGATACCCAGGCAGATAAAAATGAGAAAGAGATTTCTGGAGGTGTGTTTAAACAGAAAAATGTAGTAGTTCAAGCTCCTATGATTTCTCCTTCACGCTCACCAAAGTTTATTATGTTACCTGATGAATTGAAAGGATACTCTCACAGGCACATCAATCACAAATGTTGCAAGTCACTATGGGGTAGCCCTGTTCTGATTATCTTAATCTGGATTTCTCGTCGGGCTTTAACTCTATTGATCAAGCTTGTTCTTCAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

235

Amino Acids

27.09

Weight (kDa)

9.35

Isoelectric Point (pI)

48.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 65 - 112 1.8e-11 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 9
AciI CCGC 2 cut(s) 7, 9
AcsI RAATTY 2 cut(s) 16, 323
AcuI CTGAAG 1 cut(s) 683
AfaI GTAC 2 cut(s) 5, 237
AfiI CCNNNNNNNGG 1 cut(s) 431
AgsI TTSAA 3 cut(s) 185, 518, 577
AjnI CCWGG 1 cut(s) 453
AluBI AGCT 5 cut(s) 127, 164, 415, 521, 691
AluI AGCT 5 cut(s) 127, 164, 415, 521, 691
Alw26I GTCTC 1 cut(s) 86
ApeKI GCWGC 1 cut(s) 110
ApoI RAATTY 2 cut(s) 16, 323
AseI ATTAAT 1 cut(s) 420
AsuHPI GGTGA 2 cut(s) 257, 537
AsuII TTCGAA 2 cut(s) 202, 283
BarI GAAGNNNNNNTAC 2 cut(s) 219, 251
BbvI GCAGC 1 cut(s) 97
BccI CCATC 1 cut(s) 397
BceAI ACGGC 1 cut(s) 324
BciT130I CCWGG 1 cut(s) 455
BciVI GTATCC 1 cut(s) 575
BclI TGATCA 2 cut(s) 409, 684
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 1 cut(s) 240
BfuI GTATCC 1 cut(s) 575
BisI GCNGC 1 cut(s) 111
BlsI GCNGC 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 455
BmrFI CCNGG 1 cut(s) 455
BpmI CTGGAG 1 cut(s) 504
Bpu14I TTCGAA 2 cut(s) 202, 283
BpuEI CTTGAG 2 cut(s) 144, 150
BsaJI CCNNGG 3 cut(s) 7, 426, 453
Bsc4I CCNNNNNNNGG 1 cut(s) 431
Bse3DI GCAATG 1 cut(s) 209
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 3 cut(s) 7, 426, 453
BseLI CCNNNNNNNGG 1 cut(s) 431
BseMI GCAATG 1 cut(s) 209
BseMII CTCAG 1 cut(s) 384
BseXI GCAGC 1 cut(s) 97
BseYI CCCAGC 1 cut(s) 335
Bsh1236I CGCG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 1 cut(s) 431
BsmAI GTCTC 1 cut(s) 86
BsmBI CGTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 318
Bsp119I TTCGAA 2 cut(s) 202, 283
Bsp143I GATC 3 cut(s) 44, 409, 684
BspACI CCGC 2 cut(s) 7, 9
BspCNI CTCAG 1 cut(s) 385
BspFNI CGCG 1 cut(s) 9
BspHI TCATGA 1 cut(s) 316
BspT104I TTCGAA 2 cut(s) 202, 283
BsrDI GCAATG 1 cut(s) 209
BssECI CCNNGG 3 cut(s) 7, 426, 453
BssMI GATC 3 cut(s) 44, 409, 684
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 455
Bst4CI ACNGT 1 cut(s) 332
BstBI TTCGAA 2 cut(s) 202, 283
BstDEI CTNAG 2 cut(s) 222, 393
BstDSI CCRYGG 1 cut(s) 7
BstFNI CGCG 1 cut(s) 9
BstKTI GATC 3 cut(s) 47, 412, 687
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 3 cut(s) 44, 409, 684
BstNI CCWGG 1 cut(s) 455
BstSCI CCNGG 1 cut(s) 453
BstSFI CTRYAG 1 cut(s) 240
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 1 cut(s) 97
BsuI GTATCC 1 cut(s) 575
BtgI CCRYGG 1 cut(s) 7
CciI TCATGA 1 cut(s) 316
Cfr42I CCGCGG 1 cut(s) 10
Csp6I GTAC 2 cut(s) 4, 236
CviAII CATG 1 cut(s) 317
CviJI RGCY 9 cut(s) 58, 127, 164, 339, 415, 521, 633, 671, 691
CviKI_1 RGCY 9 cut(s) 58, 127, 164, 339, 415, 521, 633, 671, 691
CviQI GTAC 2 cut(s) 4, 236
DdeI CTNAG 2 cut(s) 222, 393
DpnI GATC 3 cut(s) 46, 411, 686
DpnII GATC 3 cut(s) 44, 409, 684
DraI TTTAAA 2 cut(s) 378, 496
Eco130I CCWWGG 1 cut(s) 426
Eco57I CTGAAG 1 cut(s) 683
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 426
ErhI CCWWGG 1 cut(s) 426
Esp3I CGTCTC 1 cut(s) 86
FaeI CATG 1 cut(s) 320
FaiI YATR 9 cut(s) 35, 84, 174, 242, 273, 318, 527, 560, 625
FaqI GGGAC 1 cut(s) 318
FatI CATG 1 cut(s) 316
FauI CCCGC 1 cut(s) 2
FbaI TGATCA 2 cut(s) 409, 684
Fnu4HI GCNGC 1 cut(s) 111
Fsp4HI GCNGC 1 cut(s) 111
FspBI CTAG 1 cut(s) 366
GluI GCNGC 1 cut(s) 111
GsaI CCCAGC 1 cut(s) 339
GsuI CTGGAG 1 cut(s) 504
Hin1II CATG 1 cut(s) 320
HincII GTYRAC 1 cut(s) 344
HindII GTYRAC 1 cut(s) 344
HindIII AAGCTT 1 cut(s) 689
HphI GGTGA 2 cut(s) 257, 537
Hpy166II GTNNAC 2 cut(s) 344, 389
Hpy188I TCNGA 1 cut(s) 642
Hpy188III TCNNGA 4 cut(s) 167, 317, 483, 655
Hpy8I GTNNAC 2 cut(s) 344, 389
Hpy99I CGWCG 1 cut(s) 669
HpyAV CCTTC 1 cut(s) 546
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4V TGCA 4 cut(s) 63, 232, 358, 615
HpyF3I CTNAG 2 cut(s) 222, 393
Hsp92II CATG 1 cut(s) 320
Ksp22I TGATCA 2 cut(s) 409, 684
KspI CCGCGG 1 cut(s) 10
Kzo9I GATC 3 cut(s) 44, 409, 684
LmnI GCTCC 2 cut(s) 124, 526
Lsp1109I GCAGC 1 cut(s) 97
MaeI CTAG 1 cut(s) 366
MaeIII GTNAC 4 cut(s) 215, 259, 561, 618
MalI GATC 3 cut(s) 46, 411, 686
MboI GATC 3 cut(s) 44, 409, 684
MboII GAAGA 1 cut(s) 689
MluCI AATT 4 cut(s) 16, 105, 323, 572
MnlI CCTC 1 cut(s) 479
MseI TTAA 9 cut(s) 14, 99, 131, 377, 420, 444, 495, 650, 674
MspA1I CMGCKG 1 cut(s) 9
MspR9I CCNGG 1 cut(s) 455
MssI GTTTAAAC 1 cut(s) 496
MvaI CCWGG 1 cut(s) 455
MvnI CGCG 1 cut(s) 9
NdeII GATC 3 cut(s) 44, 409, 684
NlaIII CATG 1 cut(s) 320
NmuCI GTSAC 1 cut(s) 618
NspV TTCGAA 2 cut(s) 202, 283
PagI TCATGA 1 cut(s) 316
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PkrI GCNGC 1 cut(s) 112
PmeI GTTTAAAC 1 cut(s) 496
PshBI ATTAAT 1 cut(s) 420
Psp6I CCWGG 1 cut(s) 453
PspFI CCCAGC 1 cut(s) 335
PspGI CCWGG 1 cut(s) 453
RsaI GTAC 2 cut(s) 5, 237
RsaNI GTAC 2 cut(s) 4, 236
SacII CCGCGG 1 cut(s) 10
SaqAI TTAA 9 cut(s) 14, 99, 131, 377, 420, 444, 495, 650, 674
SatI GCNGC 1 cut(s) 111
Sau3AI GATC 3 cut(s) 44, 409, 684
ScrFI CCNGG 1 cut(s) 455
SfcI CTRYAG 1 cut(s) 240
Sfr303I CCGCGG 1 cut(s) 10
SfuI TTCGAA 2 cut(s) 202, 283
SgrBI CCGCGG 1 cut(s) 10
SmlI CTYRAG 2 cut(s) 159, 165
SmoI CTYRAG 2 cut(s) 159, 165
Sse9I AATT 4 cut(s) 16, 105, 323, 572
SsiI CCGC 2 cut(s) 7, 9
SspI AATATT 2 cut(s) 67, 309
SspMI CTAG 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 453
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 1 cut(s) 332
TaqI TCGA 2 cut(s) 202, 283
TasI AATT 4 cut(s) 16, 105, 323, 572
Tru1I TTAA 9 cut(s) 14, 99, 131, 377, 420, 444, 495, 650, 674
Tru9I TTAA 9 cut(s) 14, 99, 131, 377, 420, 444, 495, 650, 674
TseFI GTSAC 1 cut(s) 618
TseI GCWGC 1 cut(s) 110
Tsp45I GTSAC 1 cut(s) 618
TspDTI ATGAA 1 cut(s) 585
VspI ATTAAT 1 cut(s) 420
XapI RAATTY 2 cut(s) 16, 323
XspI CTAG 1 cut(s) 366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.