AT4G04730

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005646
Physical Location & Seq
Reverse (-)
2401927 .. 2402349
423 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G04730.1

Sequence Viewer

Length: 423 bp
ATGACTGATAGCTCTCTTCCGGTTCCTTTACATCCACCTCCACGAGTGCCTACACCTCCATTGAATCCACAAGAATTGGCAGCAGTTACTCCATACTTTCCAACTTCAAGACCTTCAGCTTTCCAAGCTTTCACAGTTCCGACTACTCAAGCTGGTGTTAGACGTCATCAGGTTTTCCCTGACTCTAACATCAGTCAAGCCTCTGGAGCTGCGATTACTTATGGAGTAAGGCAAGTTTTTGAGGTGGGAAAATCATCAAGAGGTGCTCAAGATGGGGAATCTCACAATAACATGCATCTTGGGGAGTCATCTAAGAGGAAGAACTTGATCATTCTTCAGCGAGATAAGGAATCAAGACAACACAAACAACAAGAAACTGAGCAAAGAATGGGAGGTATTCTCAAACCTCCTAAGAAACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

140

Amino Acids

15.49

Weight (kDa)

10.32

Isoelectric Point (pI)

74.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 166
AclI AACGTT 1 cut(s) 418
AcuI CTGAAG 2 cut(s) 99, 320
AcyI GRCGYC 1 cut(s) 163
AgsI TTSAA 2 cut(s) 64, 108
AluBI AGCT 5 cut(s) 12, 119, 128, 152, 209
AluI AGCT 5 cut(s) 12, 119, 128, 152, 209
Alw21I GWGCWC 1 cut(s) 268
ApeKI GCWGC 2 cut(s) 80, 209
BauI CACGAG 1 cut(s) 42
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 2 cut(s) 92, 196
BccI CCATC 1 cut(s) 266
BclI TGATCA 1 cut(s) 327
BisI GCNGC 2 cut(s) 81, 210
BlsI GCNGC 2 cut(s) 82, 211
BmiI GGNNCC 1 cut(s) 24
BmsI GCATC 1 cut(s) 304
BplI GAGNNNNNCTC 2 cut(s) 384, 416
BpmI CTGGAG 1 cut(s) 225
BpuEI CTTGAG 2 cut(s) 132, 252
BsaHI GRCGYC 1 cut(s) 163
BsaWI WCCGGW 1 cut(s) 19
BseGI GGATG 1 cut(s) 31
BseMII CTCAG 1 cut(s) 369
BseXI GCAGC 2 cut(s) 92, 196
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 1 cut(s) 20
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 1 cut(s) 327
BspCNI CTCAG 1 cut(s) 370
BspLI GGNNCC 1 cut(s) 24
BssMI GATC 1 cut(s) 327
BssNI GRCGYC 1 cut(s) 163
BssSI CACGAG 1 cut(s) 42
Bst2BI CACGAG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 136
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 1 cut(s) 163
BstDEI CTNAG 3 cut(s) 312, 378, 411
BstF5I GGATG 1 cut(s) 31
BstKTI GATC 1 cut(s) 330
BstMBI GATC 1 cut(s) 327
BstMWI GCNNNNNNNGC 2 cut(s) 125, 206
BstNSI RCATGY 1 cut(s) 295
BstV1I GCAGC 2 cut(s) 92, 196
BtsCI GGATG 1 cut(s) 31
CspCI CAANNNNNGTGG 2 cut(s) 57, 92
CviAII CATG 1 cut(s) 292
CviJI RGCY 6 cut(s) 12, 119, 128, 152, 200, 209
CviKI_1 RGCY 6 cut(s) 12, 119, 128, 152, 200, 209
DdeI CTNAG 3 cut(s) 312, 378, 411
DpnI GATC 1 cut(s) 329
DpnII GATC 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Eco57I CTGAAG 2 cut(s) 99, 320
EcoT22I ATGCAT 1 cut(s) 297
FaeI CATG 1 cut(s) 295
FaiI YATR 3 cut(s) 94, 222, 293
FatI CATG 1 cut(s) 291
FbaI TGATCA 1 cut(s) 327
Fnu4HI GCNGC 2 cut(s) 81, 210
FokI GGATG 1 cut(s) 18
Fsp4HI GCNGC 2 cut(s) 81, 210
GluI GCNGC 2 cut(s) 81, 210
GsuI CTGGAG 1 cut(s) 225
HapII CCGG 1 cut(s) 20
Hin1I GRCGYC 1 cut(s) 163
Hin1II CATG 1 cut(s) 295
HindIII AAGCTT 1 cut(s) 126
HinfI GANTC 5 cut(s) 64, 182, 278, 305, 350
HpaII CCGG 1 cut(s) 20
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 5 cut(s) 108, 204, 258, 269, 354
HpyAV CCTTC 1 cut(s) 123
HpyCH4III ACNGT 1 cut(s) 136
HpyCH4IV ACGT 2 cut(s) 163, 418
HpyCH4V TGCA 1 cut(s) 295
HpyF10VI GCNNNNNNNGC 2 cut(s) 125, 206
HpyF3I CTNAG 3 cut(s) 312, 378, 411
HpySE526I ACGT 2 cut(s) 163, 418
Hsp92I GRCGYC 1 cut(s) 163
Hsp92II CATG 1 cut(s) 295
Ksp22I TGATCA 1 cut(s) 327
Kzo9I GATC 1 cut(s) 327
LmnI GCTCC 1 cut(s) 206
LpnPI CCDG 5 cut(s) 33, 138, 155, 189, 192
Lsp1109I GCAGC 2 cut(s) 92, 196
LweI GCATC 1 cut(s) 304
MaeII ACGT 2 cut(s) 163, 418
MaeIII GTNAC 1 cut(s) 85
MalI GATC 1 cut(s) 329
MboI GATC 1 cut(s) 327
MboII GAAGA 3 cut(s) 8, 326, 331
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 1 cut(s) 74
MlyI GAGTC 2 cut(s) 176, 314
MmeI TCCRAC 2 cut(s) 125, 164
MnlI CCTC 8 cut(s) 48, 66, 211, 235, 254, 309, 386, 417
Mph1103I ATGCAT 1 cut(s) 297
MseI TTAA 1 cut(s) 421
MspI CCGG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 125, 206
NdeII GATC 1 cut(s) 327
NlaIII CATG 1 cut(s) 295
NlaIV GGNNCC 1 cut(s) 24
NsiI ATGCAT 1 cut(s) 297
NspI RCATGY 1 cut(s) 295
PfeI GAWTC 3 cut(s) 64, 278, 350
PkrI GCNGC 2 cut(s) 82, 211
PleI GAGTC 2 cut(s) 176, 313
PpsI GAGTC 2 cut(s) 176, 313
Psp1406I AACGTT 1 cut(s) 418
PspN4I GGNNCC 1 cut(s) 24
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 2 cut(s) 81, 210
Sau3AI GATC 1 cut(s) 327
SchI GAGTC 2 cut(s) 176, 314
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 1 cut(s) 304
SmlI CTYRAG 2 cut(s) 147, 267
SmoI CTYRAG 2 cut(s) 147, 267
Sse9I AATT 1 cut(s) 74
TaaI ACNGT 1 cut(s) 136
TaiI ACGT 2 cut(s) 166, 421
TasI AATT 1 cut(s) 74
TfiI GAWTC 3 cut(s) 64, 278, 350
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TseI GCWGC 2 cut(s) 80, 209
XceI RCATGY 1 cut(s) 295
ZraI GACGTC 1 cut(s) 164
Zsp2I ATGCAT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.