AT2G13510

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005646
Physical Location & Seq
Reverse (-)
5630001 .. 5631107
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G13510.1

Sequence Viewer

Length: 855 bp
ATGGCGGATAAGCTGTGGGATGAGATACAAAACCTTGAGTTAGGTCCGGAGGATCCGACTCTGTTTATTCCACATGAAGCTTATGTTATGGTGGCTGAAACCAACAGGCTGAGTATAATAGCAAGACCTCTCAATCCACGGGTACAAAATCTCTACTCTGTTATTGCTGCTTTACCAAGATCTTGGGGTTTAACAACTCATGTTCATGGAAGAGTTTTAGATGCTACGTATGTGCAATTCCTGTTTCACACAGAAATGGACTTGGTCTCAGTACAAAGGAGGGAACCATGGCTTTTTAACAACAGGTTTGTTGCATCTCAAAGATGGGAGCCTGCTCCTGCTTTAAACTTTGTCACTACCATTGATCTTTTGGTTCAGATGCGTGGTATTCCCCTTCCTTATGTGTATGAAGAAACAGCTTTAGAGATTGCTCAAGAGATTGGAGAGATTATCTCTTTAGACTTTCATGATGCTACTTCAACTCAGATTGCTTATATTAGAGTCCGGGTTCAGATTGGTATCACCGAGCGTCTAAGGGTCCCAGCTCCTCCATTGAATCCAGAAGAATTAGCAGGTGCTAGTCCTTATTTTCCAAGTTCAAGAAATGCAGCTTTCCAACCTTACATGGTTCCTTCTACTCAAGGAGGTGTAGGTCGTCATCATGTCTTCCCAGACTCTAACATTAATCCTTCCTCTGGAGCTGCGATTACTTCAGGAGTTCGACGTGTTTATGAGGTAGGAGAATCATTTCGTCCTTGTCCAGATGGAGAATCTCGCAATAACAGGAACATTGGGGAGTCTTCTAAACGAAAAAACTTAGTGATTCTACAAAGTGACAATGAGAAGGCAAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

284

Amino Acids

31.93

Weight (kDa)

5.69

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 32 - 112 2.3e-16 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 563
Acc36I ACCTGC 1 cut(s) 563
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 47, 60
AcuI CTGAAG 1 cut(s) 696
AfaI GTAC 2 cut(s) 144, 273
AfiI CCNNNNNNNGG 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 724
AgsI TTSAA 3 cut(s) 480, 556, 600
AjiI CACGTC 1 cut(s) 725
AluBI AGCT 6 cut(s) 13, 80, 419, 545, 611, 701
AluI AGCT 6 cut(s) 13, 80, 419, 545, 611, 701
Alw26I GTCTC 1 cut(s) 271
AlwI GGATC 2 cut(s) 47, 60
Aor13HI TCCGGA 1 cut(s) 46
ApeKI GCWGC 3 cut(s) 167, 608, 701
AseI ATTAAT 1 cut(s) 684
Asp700I GAANNNNTTC 1 cut(s) 747
AspS9I GGNCC 2 cut(s) 44, 538
AsuC2I CCSGG 1 cut(s) 506
AsuHPI GGTGA 1 cut(s) 514
AvaII GGWCC 2 cut(s) 44, 538
BamHI GGATCC 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 658, 792
BbvI GCAGC 3 cut(s) 154, 620, 688
BccI CCATC 2 cut(s) 318, 758
BcnI CCSGG 1 cut(s) 506
BcoDI GTCTC 1 cut(s) 271
BfaI CTAG 1 cut(s) 579
BfuAI ACCTGC 1 cut(s) 563
BglII AGATCT 1 cut(s) 179
BisI GCNGC 3 cut(s) 168, 609, 702
BlsI GCNGC 3 cut(s) 169, 610, 703
Bme1390I CCNGG 1 cut(s) 506
Bme18I GGWCC 2 cut(s) 44, 538
BmgBI CACGTC 1 cut(s) 725
BmgT120I GGNCC 2 cut(s) 44, 538
BmiI GGNNCC 6 cut(s) 54, 285, 330, 539, 540, 630
BmrFI CCNGG 1 cut(s) 506
BmsI GCATC 4 cut(s) 211, 323, 369, 460
BpiI GAAGAC 2 cut(s) 658, 792
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 1 cut(s) 717
BpuEI CTTGAG 3 cut(s) 56, 417, 624
BpuMI CCSGG 1 cut(s) 506
BsaAI YACGTR 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 518
BsaI GGTCTC 1 cut(s) 271
BsaJI CCNNGG 2 cut(s) 137, 287
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 695
Bse8I GATNNNNATC 1 cut(s) 518
BseAI TCCGGA 1 cut(s) 46
BseDI CCNNGG 2 cut(s) 137, 287
BseGI GGATG 1 cut(s) 25
BseJI GATNNNNATC 1 cut(s) 518
BseLI CCNNNNNNNGG 1 cut(s) 695
BseMII CTCAG 3 cut(s) 101, 282, 497
BseRI GAGGAG 1 cut(s) 537
BseXI GCAGC 3 cut(s) 154, 620, 688
BseYI CCCAGC 1 cut(s) 541
BsiSI CCGG 2 cut(s) 47, 505
BslFI GGGAC 1 cut(s) 524
BslI CCNNNNNNNGG 1 cut(s) 695
BsmAI GTCTC 1 cut(s) 271
BsmFI GGGAC 1 cut(s) 524
Bso31I GGTCTC 1 cut(s) 271
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 3 cut(s) 52, 179, 364
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 1 cut(s) 5
BspCNI CTCAG 3 cut(s) 102, 281, 496
BspEI TCCGGA 1 cut(s) 46
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 6 cut(s) 54, 285, 330, 539, 540, 630
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 2 cut(s) 47, 60
BspTNI GGTCTC 1 cut(s) 271
BssECI CCNNGG 2 cut(s) 137, 287
BssMI GATC 3 cut(s) 52, 179, 364
BssT1I CCWWGG 1 cut(s) 287
Bst6I CTCTTC 1 cut(s) 205
BstBAI YACGTR 1 cut(s) 228
BstC8I GCNNGC 1 cut(s) 333
BstDEI CTNAG 5 cut(s) 110, 268, 483, 533, 817
BstDSI CCRYGG 2 cut(s) 137, 287
BstF5I GGATG 1 cut(s) 25
BstKTI GATC 3 cut(s) 55, 182, 367
BstMAI GTCTC 1 cut(s) 271
BstMBI GATC 3 cut(s) 52, 179, 364
BstSCI CCNGG 1 cut(s) 504
BstSNI TACGTA 1 cut(s) 228
BstV1I GCAGC 3 cut(s) 154, 620, 688
BstV2I GAAGAC 2 cut(s) 658, 792
BstX2I RGATCY 2 cut(s) 52, 179
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 2 cut(s) 52, 179
BtgI CCRYGG 2 cut(s) 137, 287
BtrI CACGTC 1 cut(s) 725
BtsCI GGATG 1 cut(s) 25
BveI ACCTGC 1 cut(s) 563
Cac8I GCNNGC 1 cut(s) 333
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 2 cut(s) 44, 538
CseI GACGC 1 cut(s) 518
Csp6I GTAC 2 cut(s) 143, 272
CviAII CATG 7 cut(s) 74, 200, 206, 288, 467, 625, 662
CviQI GTAC 2 cut(s) 143, 272
DdeI CTNAG 5 cut(s) 110, 268, 483, 533, 817
DpnI GATC 3 cut(s) 54, 181, 366
DpnII GATC 3 cut(s) 52, 179, 364
DraI TTTAAA 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 205
EarI CTCTTC 1 cut(s) 205
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 287
Eco31I GGTCTC 1 cut(s) 271
Eco47I GGWCC 2 cut(s) 44, 538
Eco57I CTGAAG 1 cut(s) 696
EcoO109I RGGNCCY 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
FaeI CATG 7 cut(s) 77, 203, 209, 291, 470, 628, 665
FaqI GGGAC 1 cut(s) 524
FatI CATG 7 cut(s) 73, 199, 205, 287, 466, 624, 661
Fnu4HI GCNGC 3 cut(s) 168, 609, 702
FokI GGATG 1 cut(s) 32
Fsp4HI GCNGC 3 cut(s) 168, 609, 702
FspBI CTAG 1 cut(s) 579
GluI GCNGC 3 cut(s) 168, 609, 702
GsaI CCCAGC 1 cut(s) 545
GsuI CTGGAG 1 cut(s) 717
HapII CCGG 2 cut(s) 47, 505
HgaI GACGC 1 cut(s) 518
Hin1II CATG 7 cut(s) 77, 203, 209, 291, 470, 628, 665
HindIII AAGCTT 1 cut(s) 78
HinfI GANTC 8 cut(s) 58, 501, 556, 674, 743, 770, 797, 823
HpaII CCGG 2 cut(s) 47, 505
HphI GGTGA 1 cut(s) 514
Hpy188I TCNGA 4 cut(s) 57, 378, 486, 513
Hpy188III TCNNGA 8 cut(s) 47, 434, 467, 560, 600, 696, 714, 761
Hpy99I CGWCG 1 cut(s) 726
HpyAV CCTTC 4 cut(s) 404, 642, 699, 838
HpyCH4IV ACGT 2 cut(s) 227, 724
HpyCH4V TGCA 3 cut(s) 235, 314, 608
HpyF3I CTNAG 5 cut(s) 110, 268, 483, 533, 817
HpySE526I ACGT 2 cut(s) 227, 724
Hsp92II CATG 7 cut(s) 77, 203, 209, 291, 470, 628, 665
KflI GGGWCCC 1 cut(s) 538
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 3 cut(s) 52, 179, 364
LmnI GCTCC 4 cut(s) 328, 340, 550, 698
Lsp1109I GCAGC 3 cut(s) 154, 620, 688
LweI GCATC 4 cut(s) 211, 323, 369, 460
MaeI CTAG 1 cut(s) 579
MaeII ACGT 2 cut(s) 227, 724
MaeIII GTNAC 2 cut(s) 352, 833
MalI GATC 3 cut(s) 54, 181, 366
MboI GATC 3 cut(s) 52, 179, 364
MboII GAAGA 5 cut(s) 222, 422, 575, 658, 792
MflI RGATCY 2 cut(s) 52, 179
MluCI AATT 2 cut(s) 236, 566
MlyI GAGTC 4 cut(s) 52, 510, 668, 806
MmeI TCCRAC 2 cut(s) 80, 640
MnlI CCTC 7 cut(s) 43, 138, 273, 558, 638, 703, 727
MroI TCCGGA 1 cut(s) 46
MroXI GAANNNNTTC 1 cut(s) 747
MseI TTAA 4 cut(s) 191, 297, 344, 684
MslI CAYNNNNRTG 2 cut(s) 204, 254
MspI CCGG 2 cut(s) 47, 505
MspR9I CCNGG 1 cut(s) 506
NciI CCSGG 1 cut(s) 506
NcoI CCATGG 1 cut(s) 287
NdeII GATC 3 cut(s) 52, 179, 364
NlaIII CATG 7 cut(s) 77, 203, 209, 291, 470, 628, 665
NlaIV GGNNCC 6 cut(s) 54, 285, 330, 539, 540, 630
NmuCI GTSAC 2 cut(s) 352, 833
PagI TCATGA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 563
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 4 cut(s) 556, 743, 770, 823
PflFI GACNNNGTC 1 cut(s) 263
PkrI GCNGC 3 cut(s) 169, 610, 703
PleI GAGTC 4 cut(s) 52, 509, 668, 805
PpsI GAGTC 4 cut(s) 52, 509, 668, 805
Ppu21I YACGTR 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 538
PshBI ATTAAT 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 538
PspFI CCCAGC 1 cut(s) 541
PspN4I GGNNCC 6 cut(s) 54, 285, 330, 539, 540, 630
PspPI GGNCC 2 cut(s) 44, 538
PspPPI RGGWCCY 1 cut(s) 538
PsuI RGATCY 2 cut(s) 52, 179
PsyI GACNNNGTC 1 cut(s) 263
RsaI GTAC 2 cut(s) 144, 273
RsaNI GTAC 2 cut(s) 143, 272
RseI CAYNNNNRTG 2 cut(s) 204, 254
SaqAI TTAA 4 cut(s) 191, 297, 344, 684
SatI GCNGC 3 cut(s) 168, 609, 702
Sau3AI GATC 3 cut(s) 52, 179, 364
Sau96I GGNCC 2 cut(s) 44, 538
SchI GAGTC 4 cut(s) 52, 510, 668, 806
ScrFI CCNGG 1 cut(s) 506
SfaNI GCATC 4 cut(s) 211, 323, 369, 460
SinI GGWCC 2 cut(s) 44, 538
SmiMI CAYNNNNRTG 2 cut(s) 204, 254
SmlI CTYRAG 3 cut(s) 35, 432, 639
SmoI CTYRAG 3 cut(s) 35, 432, 639
SnaBI TACGTA 1 cut(s) 228
Sse9I AATT 2 cut(s) 236, 566
SsiI CCGC 1 cut(s) 5
SspMI CTAG 1 cut(s) 579
StyD4I CCNGG 1 cut(s) 504
StyI CCWWGG 1 cut(s) 287
TaiI ACGT 2 cut(s) 230, 727
TaqI TCGA 1 cut(s) 721
TasI AATT 2 cut(s) 236, 566
TatI WGTACW 1 cut(s) 271
TfiI GAWTC 4 cut(s) 556, 743, 770, 823
Tru1I TTAA 4 cut(s) 191, 297, 344, 684
Tru9I TTAA 4 cut(s) 191, 297, 344, 684
TseFI GTSAC 2 cut(s) 352, 833
TseI GCWGC 3 cut(s) 167, 608, 701
Tsp45I GTSAC 2 cut(s) 352, 833
TspDTI ATGAA 4 cut(s) 90, 194, 423, 455
Tth111I GACNNNGTC 1 cut(s) 263
VpaK11BI GGWCC 2 cut(s) 44, 538
VspI ATTAAT 1 cut(s) 684
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 1 cut(s) 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.