AT3G47920

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005646
Physical Location & Seq
Forward (+)
17682952 .. 17683854
903 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G47920.1

Sequence Viewer

Length: 903 bp
ATGCTGGATGCTACTTATGTTCAGTTCTTGTTTCAGAATGAAGTTGATTTACTTTCAGTGCAAAGAAGGGAACTGTGGCTTTTCAATAATTGGTTTGTTGCAAATCATAGATGGGAGCCTGCTCCTGTCTTAAATTTTGTGACTACCATAGATCTTTGGGTGCAGATGCGAGGCATTCCTCTTCTCTATGTTTGTGAGGAGACAGCTTTGGAGATTGCTCATGAGATTGGTGAGATCATCACGCTGGACTTCCATGATGCAACGATGACTCAAATTGCTTATATTAGAGTCAGAGTTAGAATTGGAATCACTGATAGGCTTCGTTTCTTCCAAAGAATCACTTTTGATTCTGGGGAAACTGCTCTCATCAGGTTCCAATATGAGAGATTGAGGAGGATTTGTAGTAGCTGCTTCAGAGTTACACATCACAGGAACTATTGCCCTTATAGACCAAGGCTACCTAATTATGGAAGGGAAAGAGCTGTCTTCCATGATGAAAGGCTTAGATCGTCTATGAATTCTCAGTCTCAAATGACGGAGTCTTCATTTCCAGCACCTGTGCTCCCTCCACCAAGAATTGTTACCCCTCCACTGAATCATGGAGAGTTTCTTGCAGCCCATCCGAATTTTCCTGCTCCAAGAGAAGGCCTTAATCATCAGGGTCGAGGGACTTACTATCAAGGGCTCTGTCAAGGTCAACAGGTCTCAACTGATTCTAATATCACGCCTTCAGTTGGAACAGCACTCTCAACGGGTTCTCGTCGGGTTTTTGAAGTTGGTCAATCTTCAAGAGGGGTGGAAACAAGGGAAACAAGGAAGAGGCAAGAAGAGAAAGGAACTCATGATGAACAAGACAAAGCGCACATGAAGGGAGGGATTCTGAACCCTCCAAAGAAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

300

Amino Acids

34.76

Weight (kDa)

9.18

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 2 - 41 2e-06 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 101 - 149 1.6e-22 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 3 cut(s) 133, 517, 625
AcuI CTGAAG 2 cut(s) 397, 714
AfiI CCNNNNNNNGG 3 cut(s) 467, 644, 734
AgsI TTSAA 3 cut(s) 85, 773, 789
AluBI AGCT 3 cut(s) 206, 408, 482
AluI AGCT 3 cut(s) 206, 408, 482
Alw21I GWGCWC 1 cut(s) 564
Alw26I GTCTC 3 cut(s) 194, 531, 709
AlwNI CAGNNNCTG 1 cut(s) 557
AoxI GGCC 1 cut(s) 646
ApeKI GCWGC 2 cut(s) 408, 614
ApoI RAATTY 3 cut(s) 133, 517, 625
AspLEI GCGC 1 cut(s) 862
AsuHPI GGTGA 1 cut(s) 242
BanII GRGCYC 1 cut(s) 687
BarI GAAGNNNNNNTAC 2 cut(s) 33, 65
BbsI GAAGAC 2 cut(s) 478, 534
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 2 cut(s) 395, 626
BccI CCATC 2 cut(s) 105, 627
BcoDI GTCTC 3 cut(s) 194, 531, 709
BglII AGATCT 1 cut(s) 151
BisI GCNGC 2 cut(s) 409, 615
BlsI GCNGC 2 cut(s) 410, 616
BmiI GGNNCC 2 cut(s) 117, 374
BmsI GCATC 2 cut(s) 156, 247
BpiI GAAGAC 2 cut(s) 478, 534
BsaI GGTCTC 1 cut(s) 709
BsaJI CCNNGG 1 cut(s) 452
BsaXI ACNNNNNCTCC 4 cut(s) 385, 415, 546, 576
Bsc4I CCNNNNNNNGG 3 cut(s) 467, 644, 734
BseDI CCNNGG 1 cut(s) 452
BseGI GGATG 2 cut(s) 13, 619
BseLI CCNNNNNNNGG 3 cut(s) 467, 644, 734
BseMII CTCAG 1 cut(s) 536
BseRI GAGGAG 2 cut(s) 212, 406
BseXI GCAGC 2 cut(s) 395, 626
BsgI GTGCAG 1 cut(s) 182
BshFI GGCC 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 564
BslFI GGGAC 1 cut(s) 682
BslI CCNNNNNNNGG 3 cut(s) 467, 644, 734
BsmAI GTCTC 3 cut(s) 194, 531, 709
BsmFI GGGAC 1 cut(s) 682
BsmI GAATGC 1 cut(s) 174
BsnI GGCC 1 cut(s) 648
Bso31I GGTCTC 1 cut(s) 709
Bsp1286I GDGCHC 2 cut(s) 564, 687
Bsp143I GATC 3 cut(s) 151, 234, 506
BspANI GGCC 1 cut(s) 648
BspCNI CTCAG 1 cut(s) 535
BspHI TCATGA 2 cut(s) 220, 841
BspLI GGNNCC 2 cut(s) 117, 374
BspTNI GGTCTC 1 cut(s) 709
BssECI CCNNGG 1 cut(s) 452
BssMI GATC 3 cut(s) 151, 234, 506
BssT1I CCWWGG 1 cut(s) 452
Bst4CI ACNGT 1 cut(s) 75
Bst6I CTCTTC 3 cut(s) 186, 812, 822
BstC8I GCNNGC 1 cut(s) 120
BstDEI CTNAG 2 cut(s) 503, 522
BstF5I GGATG 2 cut(s) 13, 619
BstHHI GCGC 1 cut(s) 862
BstKTI GATC 3 cut(s) 154, 237, 509
BstMAI GTCTC 3 cut(s) 194, 531, 709
BstMBI GATC 3 cut(s) 151, 234, 506
BstV1I GCAGC 2 cut(s) 395, 626
BstV2I GAAGAC 2 cut(s) 478, 534
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BsuRI GGCC 1 cut(s) 648
BtsCI GGATG 2 cut(s) 13, 619
BtsIMutI CAGTG 3 cut(s) 63, 309, 590
Cac8I GCNNGC 1 cut(s) 120
CaiI CAGNNNCTG 1 cut(s) 557
CciI TCATGA 2 cut(s) 220, 841
CfoI GCGC 1 cut(s) 862
CspCI CAANNNNNGTGG 2 cut(s) 777, 812
CviAII CATG 6 cut(s) 221, 254, 491, 599, 842, 865
DdeI CTNAG 2 cut(s) 503, 522
DpnI GATC 3 cut(s) 153, 236, 508
DpnII GATC 3 cut(s) 151, 234, 506
Eam1104I CTCTTC 3 cut(s) 186, 812, 822
EarI CTCTTC 3 cut(s) 186, 812, 822
Eco130I CCWWGG 1 cut(s) 452
Eco147I AGGCCT 1 cut(s) 648
Eco24I GRGCYC 1 cut(s) 687
Eco31I GGTCTC 1 cut(s) 709
Eco57I CTGAAG 2 cut(s) 397, 714
EcoRI GAATTC 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 687
ErhI CCWWGG 1 cut(s) 452
FaeI CATG 6 cut(s) 224, 257, 494, 602, 845, 868
FalI AAGNNNNNCTT 2 cut(s) 325, 357
FaqI GGGAC 1 cut(s) 682
FatI CATG 6 cut(s) 220, 253, 490, 598, 841, 864
Fnu4HI GCNGC 2 cut(s) 409, 615
FokI GGATG 2 cut(s) 20, 606
FriOI GRGCYC 1 cut(s) 687
Fsp4HI GCNGC 2 cut(s) 409, 615
GlaI GCGC 1 cut(s) 861
GluI GCNGC 2 cut(s) 409, 615
HaeIII GGCC 1 cut(s) 648
HhaI GCGC 1 cut(s) 862
Hin1II CATG 6 cut(s) 224, 257, 494, 602, 845, 868
Hin6I GCGC 1 cut(s) 860
HinP1I GCGC 1 cut(s) 860
HincII GTYRAC 1 cut(s) 698
HindII GTYRAC 1 cut(s) 698
HinfI GANTC 9 cut(s) 268, 288, 306, 336, 347, 539, 595, 713, 877
HphI GGTGA 1 cut(s) 242
Hpy166II GTNNAC 1 cut(s) 698
Hpy188I TCNGA 5 cut(s) 36, 293, 416, 624, 882
Hpy188III TCNNGA 3 cut(s) 221, 789, 842
Hpy8I GTNNAC 1 cut(s) 698
Hpy99I CGWCG 1 cut(s) 765
HpyAV CCTTC 5 cut(s) 60, 465, 638, 738, 862
HpyCH4III ACNGT 1 cut(s) 75
HpyCH4V TGCA 5 cut(s) 61, 101, 163, 260, 614
HpyF3I CTNAG 2 cut(s) 503, 522
Hsp92II CATG 6 cut(s) 224, 257, 494, 602, 845, 868
HspAI GCGC 1 cut(s) 860
Kzo9I GATC 3 cut(s) 151, 234, 506
LmnI GCTCC 4 cut(s) 115, 127, 567, 640
Lsp1109I GCAGC 2 cut(s) 395, 626
LweI GCATC 2 cut(s) 156, 247
MaeIII GTNAC 3 cut(s) 139, 418, 580
MalI GATC 3 cut(s) 153, 236, 508
MboI GATC 3 cut(s) 151, 234, 506
MboII GAAGA 7 cut(s) 173, 319, 478, 534, 777, 829, 839
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 2 cut(s) 564, 687
MluCI AATT 8 cut(s) 88, 133, 273, 300, 463, 517, 576, 625
MlyI GAGTC 3 cut(s) 262, 297, 548
MmeI TCCRAC 1 cut(s) 715
MseI TTAA 2 cut(s) 131, 651
Mva1269I GAATGC 1 cut(s) 174
NdeII GATC 3 cut(s) 151, 234, 506
NlaIII CATG 6 cut(s) 224, 257, 494, 602, 845, 868
NlaIV GGNNCC 2 cut(s) 117, 374
NmuCI GTSAC 1 cut(s) 139
PagI TCATGA 2 cut(s) 220, 841
PceI AGGCCT 1 cut(s) 648
PctI GAATGC 1 cut(s) 174
PfeI GAWTC 6 cut(s) 306, 336, 347, 595, 713, 877
PflFI GACNNNGTC 1 cut(s) 538
PkrI GCNGC 2 cut(s) 410, 616
PleI GAGTC 3 cut(s) 262, 296, 547
PpsI GAGTC 3 cut(s) 262, 296, 547
PspN4I GGNNCC 2 cut(s) 117, 374
PstNI CAGNNNCTG 1 cut(s) 557
PsuI RGATCY 1 cut(s) 151
PsyI GACNNNGTC 1 cut(s) 538
SaqAI TTAA 2 cut(s) 131, 651
SatI GCNGC 2 cut(s) 409, 615
Sau3AI GATC 3 cut(s) 151, 234, 506
SchI GAGTC 3 cut(s) 262, 297, 548
SduI GDGCHC 2 cut(s) 564, 687
SetI ASST 8 cut(s) 208, 374, 410, 463, 484, 559, 697, 705
SfaNI GCATC 2 cut(s) 156, 247
Sse9I AATT 8 cut(s) 88, 133, 273, 300, 463, 517, 576, 625
SseBI AGGCCT 1 cut(s) 648
StuI AGGCCT 1 cut(s) 648
StyI CCWWGG 1 cut(s) 452
TaaI ACNGT 1 cut(s) 75
TaqI TCGA 1 cut(s) 664
TasI AATT 8 cut(s) 88, 133, 273, 300, 463, 517, 576, 625
TfiI GAWTC 6 cut(s) 306, 336, 347, 595, 713, 877
Tru1I TTAA 2 cut(s) 131, 651
Tru9I TTAA 2 cut(s) 131, 651
TscAI CASTG 3 cut(s) 63, 316, 597
TseFI GTSAC 1 cut(s) 139
TseI GCWGC 2 cut(s) 408, 614
Tsp45I GTSAC 1 cut(s) 139
TspDTI ATGAA 6 cut(s) 54, 510, 530, 534, 861, 881
TspGWI ACGGA 1 cut(s) 551
TspRI CASTG 3 cut(s) 63, 316, 597
Tth111I GACNNNGTC 1 cut(s) 538
XapI RAATTY 3 cut(s) 133, 517, 625
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.