AT3G31430

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005646
Physical Location & Seq
Reverse (-)
12795861 .. 12796871
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G31430.1

Sequence Viewer

Length: 1011 bp
ATGGCGACAGATAAGGCGATCTGTCGACGGTTACTTTACTATCTCTTTGTCACCCCTTTGGTGACGGTTTACAGGGAATTCGCTGGGAAAGTGGTCTGTTTCTTCACCGCCCACTCCCTGGTAGTTATCTCCACGGTGGAATCCACGATTCATCGTGATGCTACTTCTTCGGCTTCAGGCAACGTTCTATTAAAGTTTCGATTTACAATCCCAGAATCCACAAATCTCATCTCTCTTAAACCTCCAAACCACTTCCCTTTACGATTCTACCAACCTCTATCACAAATGGCAGACAATCTCCGTCGTGCGGTTCAAGACATCAATTTGGGAGTTGATGACATTCCTTTTGCTCTACCGGAAGACATTGTGAATCATGCGGTTGCAGAAAACCGCTTCATCCTGTTTGGCCGGCCGGTTATGCCCAGAAGGCAAAATCTCCGTTCCATTGTAGCATCTATGCCTCGAATCTGGGGTCAGAGCGGGCTTGTCCATGGCAGAATCATGGAAGGACGCCAGTTTCATTTCATCTTTACGTTAGAGGAGTCTCTCGAAACGGTGCTACGTCGTGGTCCCTGGGCTTTCAACGATTGGATGATTCTTCTTCAGCGATGGGAACCTCAGATTCCTCTCTTCCCTTTTATCCCTTTCTGGGTTCAGATTAGAGGAATCCCTTTCCAATTTCTCAATCGAGGTGTGGTTGAGCACATTGGCAGAGCTTTGGGTCAAGTGTTGGATACTGATTTCAATGTGGAAGTTGTAGCCAGAATGGACTTTGCCAGGGTTCTTCTACATTGGGATATCACCCACCCTCTTCGCTTCCAACGACATTTCCAATTCACGGCTGGCGTCAACACTCTCCTCAGGTTTAGATATGAGCGTCTCCGTGGTTTCTGTGAGGTATGTGGAATGTTAACACACGACTTTGGTGCCTGTTTGATTCAAAATGGTGGTGAAGAGCAAGCTGATGATGATGATGACGACGAGGAACACCCGCAGACATACCATAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 Exo_endo_phos_2 RNase_H RVT_1 zf-CCHC_4

Protein Analysis

336

Amino Acids

38.97

Weight (kDa)

6.5

Isoelectric Point (pI)

41.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 129 - 207 5.8e-16 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 265 - 312 2.4e-22 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 926
AccB7I CCANNNNNTGG 1 cut(s) 118
AccBSI CCGCTC 1 cut(s) 480
AccI GTMKAC 1 cut(s) 25
AciI CCGC 6 cut(s) 108, 308, 377, 391, 480, 992
AclI AACGTT 1 cut(s) 183
AcoI YGGCCR 2 cut(s) 406, 410
AcsI RAATTY 1 cut(s) 77
AcuI CTGAAG 2 cut(s) 159, 587
AcyI GRCGYC 2 cut(s) 511, 846
AfiI CCNNNNNNNGG 4 cut(s) 118, 307, 649, 838
AgsI TTSAA 4 cut(s) 314, 583, 745, 941
AjnI CCWGG 3 cut(s) 117, 572, 776
AluBI AGCT 2 cut(s) 716, 962
AluI AGCT 2 cut(s) 716, 962
Alw21I GWGCWC 1 cut(s) 705
Alw26I GTCTC 2 cut(s) 549, 884
AoxI GGCC 2 cut(s) 406, 410
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 4 cut(s) 329, 361, 457, 489
AspS9I GGNCC 1 cut(s) 569
AsuHPI GGTGA 5 cut(s) 43, 73, 97, 793, 962
AvaII GGWCC 1 cut(s) 569
AxyI CCTNAGG 1 cut(s) 860
BanI GGYRCC 1 cut(s) 926
BbsI GAAGAC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 705
BccI CCATC 1 cut(s) 603
BceAI ACGGC 1 cut(s) 855
BcgI CGANNNNNNTGC 2 cut(s) 908, 942
BciT130I CCWGG 3 cut(s) 119, 574, 778
BciVI GTATCC 1 cut(s) 727
BcoDI GTCTC 2 cut(s) 549, 884
BfuI GTATCC 1 cut(s) 727
BglI GCCNNNNNGGC 1 cut(s) 427
Bme1390I CCNGG 3 cut(s) 119, 574, 778
Bme18I GGWCC 1 cut(s) 569
BmgT120I GGNCC 1 cut(s) 569
BmiI GGNNCC 3 cut(s) 571, 615, 928
BmrFI CCNGG 3 cut(s) 119, 574, 778
BmsI GCATC 2 cut(s) 148, 461
BpiI GAAGAC 1 cut(s) 366
BsaHI GRCGYC 2 cut(s) 511, 846
BsaJI CCNNGG 7 cut(s) 117, 132, 490, 572, 573, 777, 883
BsaWI WCCGGW 1 cut(s) 355
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 307, 649, 838
Bse118I RCCGGY 2 cut(s) 408, 412
Bse1I ACTGG 1 cut(s) 514
Bse21I CCTNAGG 1 cut(s) 860
BseBI CCWGG 3 cut(s) 119, 574, 778
BseDI CCNNGG 7 cut(s) 117, 132, 490, 572, 573, 777, 883
BseGI GGATG 2 cut(s) 396, 597
BseLI CCNNNNNNNGG 4 cut(s) 118, 307, 649, 838
BseMII CTCAG 2 cut(s) 632, 874
BseNI ACTGG 1 cut(s) 514
BseRI GAGGAG 2 cut(s) 554, 848
BseX3I CGGCCG 1 cut(s) 410
BseYI CCCAGC 1 cut(s) 83
Bsh1285I CGRYCG 1 cut(s) 413
BshFI GGCC 2 cut(s) 408, 412
BshNI GGYRCC 1 cut(s) 926
BsiEI CGRYCG 1 cut(s) 413
BsiHKAI GWGCWC 1 cut(s) 705
BsiSI CCGG 3 cut(s) 356, 409, 413
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 4 cut(s) 118, 307, 649, 838
BsmAI GTCTC 2 cut(s) 549, 884
BsmBI CGTCTC 1 cut(s) 884
BsmFI GGGAC 1 cut(s) 555
BsnI GGCC 2 cut(s) 408, 412
Bsp1286I GDGCHC 1 cut(s) 705
Bsp143I GATC 1 cut(s) 18
Bsp19I CCATGG 1 cut(s) 490
BspACI CCGC 6 cut(s) 108, 308, 377, 391, 480, 992
BspANI GGCC 2 cut(s) 408, 412
BspCNI CTCAG 2 cut(s) 631, 873
BspLI GGNNCC 3 cut(s) 571, 615, 928
BspQI GCTCTTC 1 cut(s) 948
BspT107I GGYRCC 1 cut(s) 926
BsrBI CCGCTC 1 cut(s) 480
BsrFI RCCGGY 2 cut(s) 408, 412
BsrI ACTGG 1 cut(s) 514
BssAI RCCGGY 2 cut(s) 408, 412
BssECI CCNNGG 7 cut(s) 117, 132, 490, 572, 573, 777, 883
BssMI GATC 1 cut(s) 18
BssNI GRCGYC 2 cut(s) 511, 846
BssT1I CCWWGG 1 cut(s) 490
Bst2UI CCWGG 3 cut(s) 119, 574, 778
Bst4CI ACNGT 4 cut(s) 30, 67, 136, 556
Bst6I CTCTTC 3 cut(s) 635, 816, 948
BstACI GRCGYC 2 cut(s) 511, 846
BstC8I GCNNGC 4 cut(s) 410, 482, 844, 960
BstDEI CTNAG 2 cut(s) 618, 860
BstDSI CCRYGG 3 cut(s) 132, 490, 883
BstF5I GGATG 2 cut(s) 396, 597
BstKTI GATC 1 cut(s) 21
BstMAI GTCTC 2 cut(s) 549, 884
BstMBI GATC 1 cut(s) 18
BstMCI CGRYCG 1 cut(s) 413
BstMWI GCNNNNNNNGC 2 cut(s) 418, 427
BstNI CCWGG 3 cut(s) 119, 574, 778
BstSCI CCNGG 3 cut(s) 117, 572, 776
BstV2I GAAGAC 1 cut(s) 366
BstZI CGGCCG 1 cut(s) 410
Bsu36I CCTNAGG 1 cut(s) 860
BsuI GTATCC 1 cut(s) 727
BsuRI GGCC 2 cut(s) 408, 412
BtgI CCRYGG 3 cut(s) 132, 490, 883
BtgZI GCGATG 1 cut(s) 622
BtsCI GGATG 2 cut(s) 396, 597
Cac8I GCNNGC 4 cut(s) 410, 482, 844, 960
Cfr10I RCCGGY 2 cut(s) 408, 412
Cfr13I GGNCC 1 cut(s) 569
CseI GACGC 3 cut(s) 519, 835, 866
CviAII CATG 3 cut(s) 374, 491, 502
CviJI RGCY 9 cut(s) 173, 408, 412, 484, 578, 716, 761, 842, 962
CviKI_1 RGCY 9 cut(s) 173, 408, 412, 484, 578, 716, 761, 842, 962
DdeI CTNAG 2 cut(s) 618, 860
DpnI GATC 1 cut(s) 20
DpnII GATC 1 cut(s) 18
EaeI YGGCCR 2 cut(s) 406, 410
EagI CGGCCG 1 cut(s) 410
Eam1104I CTCTTC 3 cut(s) 635, 816, 948
EarI CTCTTC 3 cut(s) 635, 816, 948
EclXI CGGCCG 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 490
Eco32I GATATC 1 cut(s) 799
Eco47I GGWCC 1 cut(s) 569
Eco52I CGGCCG 1 cut(s) 410
Eco57I CTGAAG 2 cut(s) 159, 587
Eco81I CCTNAGG 1 cut(s) 860
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 3 cut(s) 117, 572, 776
EcoRV GATATC 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
Esp3I CGTCTC 1 cut(s) 884
FaeI CATG 3 cut(s) 377, 494, 505
FaiI YATR 9 cut(s) 375, 419, 458, 492, 503, 873, 901, 1000, 1005
FaqI GGGAC 1 cut(s) 555
FatI CATG 3 cut(s) 373, 490, 501
FauI CCCGC 2 cut(s) 473, 999
FblI GTMKAC 1 cut(s) 25
FokI GGATG 2 cut(s) 383, 604
FseI GGCCGGCC 1 cut(s) 412
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 2 cut(s) 408, 412
HapII CCGG 3 cut(s) 356, 409, 413
HgaI GACGC 3 cut(s) 519, 835, 866
Hin1I GRCGYC 2 cut(s) 511, 846
Hin1II CATG 3 cut(s) 377, 494, 505
HincII GTYRAC 3 cut(s) 26, 850, 912
HindII GTYRAC 3 cut(s) 26, 850, 912
HpaI GTTAAC 1 cut(s) 912
HpaII CCGG 3 cut(s) 356, 409, 413
HphI GGTGA 5 cut(s) 43, 73, 97, 793, 962
Hpy166II GTNNAC 4 cut(s) 26, 70, 850, 912
Hpy188I TCNGA 3 cut(s) 477, 621, 657
Hpy188III TCNNGA 3 cut(s) 155, 314, 548
Hpy8I GTNNAC 4 cut(s) 26, 70, 850, 912
Hpy99I CGWCG 4 cut(s) 30, 306, 567, 983
HpyAV CCTTC 2 cut(s) 420, 500
HpyCH4III ACNGT 4 cut(s) 30, 67, 136, 556
HpyCH4IV ACGT 3 cut(s) 183, 533, 562
HpyCH4V TGCA 1 cut(s) 383
HpyF10VI GCNNNNNNNGC 2 cut(s) 418, 427
HpyF3I CTNAG 2 cut(s) 618, 860
HpySE526I ACGT 3 cut(s) 183, 533, 562
Hsp92I GRCGYC 2 cut(s) 511, 846
Hsp92II CATG 3 cut(s) 377, 494, 505
KroI GCCGGC 1 cut(s) 408
KroNI GCCGGC 1 cut(s) 410
KspAI GTTAAC 1 cut(s) 912
Kzo9I GATC 1 cut(s) 18
LguI GCTCTTC 1 cut(s) 948
LweI GCATC 2 cut(s) 148, 461
MaeII ACGT 3 cut(s) 183, 533, 562
MaeIII GTNAC 3 cut(s) 30, 49, 61
MalI GATC 1 cut(s) 20
MbiI CCGCTC 1 cut(s) 480
MboI GATC 1 cut(s) 18
MboII GAAGA 9 cut(s) 94, 159, 371, 590, 593, 622, 776, 803, 965
MhlI GDGCHC 1 cut(s) 705
MluCI AATT 5 cut(s) 77, 322, 677, 833, 1006
MlyI GAGTC 1 cut(s) 551
MmeI TCCRAC 2 cut(s) 711, 844
MroNI GCCGGC 1 cut(s) 408
MseI TTAA 3 cut(s) 191, 237, 911
MslI CAYNNNNRTG 1 cut(s) 156
MspI CCGG 3 cut(s) 356, 409, 413
MspR9I CCNGG 3 cut(s) 119, 574, 778
MvaI CCWGG 3 cut(s) 119, 574, 778
MwoI GCNNNNNNNGC 2 cut(s) 418, 427
NaeI GCCGGC 1 cut(s) 410
NcoI CCATGG 1 cut(s) 490
NdeII GATC 1 cut(s) 18
NgoMIV GCCGGC 1 cut(s) 408
NlaIII CATG 3 cut(s) 377, 494, 505
NlaIV GGNNCC 3 cut(s) 571, 615, 928
NmuCI GTSAC 2 cut(s) 49, 61
PasI CCCWGGG 1 cut(s) 573
PciSI GCTCTTC 1 cut(s) 948
PcsI WCGNNNNNNNCGW 1 cut(s) 820
PdiI GCCGGC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 118
PleI GAGTC 1 cut(s) 550
PpsI GAGTC 1 cut(s) 550
Psp1406I AACGTT 1 cut(s) 183
Psp6I CCWGG 3 cut(s) 117, 572, 776
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 3 cut(s) 117, 572, 776
PspN4I GGNNCC 3 cut(s) 571, 615, 928
PspPI GGNCC 1 cut(s) 569
RigI GGCCGGCC 1 cut(s) 412
RseI CAYNNNNRTG 1 cut(s) 156
SalI GTCGAC 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 948
SaqAI TTAA 3 cut(s) 191, 237, 911
Sau3AI GATC 1 cut(s) 18
Sau96I GGNCC 1 cut(s) 569
SchI GAGTC 1 cut(s) 551
ScrFI CCNGG 3 cut(s) 119, 574, 778
SduI GDGCHC 1 cut(s) 705
SfaNI GCATC 2 cut(s) 148, 461
SinI GGWCC 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 156
Sse9I AATT 5 cut(s) 77, 322, 677, 833, 1006
SsiI CCGC 6 cut(s) 108, 308, 377, 391, 480, 992
StyD4I CCNGG 3 cut(s) 117, 572, 776
StyI CCWWGG 1 cut(s) 490
TaaI ACNGT 4 cut(s) 30, 67, 136, 556
TaiI ACGT 3 cut(s) 186, 536, 565
TaqI TCGA 5 cut(s) 25, 199, 463, 549, 688
TasI AATT 5 cut(s) 77, 322, 677, 833, 1006
Tru1I TTAA 3 cut(s) 191, 237, 911
Tru9I TTAA 3 cut(s) 191, 237, 911
TseFI GTSAC 2 cut(s) 49, 61
Tsp45I GTSAC 2 cut(s) 49, 61
TspDTI ATGAA 4 cut(s) 140, 385, 509, 514
TspGWI ACGGA 3 cut(s) 290, 428, 872
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 1 cut(s) 569
XapI RAATTY 1 cut(s) 77
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XmiI GTMKAC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.