AT5G25200

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005646
Physical Location & Seq
Reverse (-)
8716766 .. 8717869
1104 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G25200.1

Sequence Viewer

Length: 1104 bp
ATGGCAGATGAGTTGTGGGATGAGATACAAAATCTAGAATTAGGACAAGAGGATCCAGCCCTTTTCATTCCCCATGAAGCTTATGTGATGGTTGAAGCTTCAAATAACTTAAGTCTGATAGCGAGACCTCTGAACCCAAGAGTTCAAAATCTTAACTCTGTCGTGGTAGCACTTCCAAGATCATGGGGACTAACGACTCAGGTGCATGGAAGAGTTCTTGATGCAACGTATGTCCAGTTCCTTTTTGCAAATGAAATTGATCTGATGATGGTGCAAAGAAGGGAACCGTGGCTTTTCAACAATTGGTTTGTGGCAGCAACTCGATGGCAGGTGGCTCCAGCTCACAACCTTGTCACTACCATTGATCTCTGGGTGCAGATTAGAGGTATTCCCTTACCTTATGTTTCTGAAGAGACGGTTTTGGAGATAGCTCAAGATCTAGGAGAGATCATTTCACTAGACTTTCATGAGGCTACCTCTCCTCAGATTGCTTTTATTAGAGTTAGAGTTCGTTTTGGAATAACAGACAGACTGAGGTTTTTTCAAAGAATCATCTTCGATTCTGGTGAAACTGCAACCATAAGGTTCCAATACGAACGTCTCCGAAGACTCTGCAGTAGCTGTTTCAGGTTCACTCACAATCGAGCTTACTGTCCTTATCGTCAACGTCCACTCAGCATCGCAAGAGAAAGAGCCCTATTCTGTGATAGTGTTCAAAGATCATCAATGAATTCCCAGTCTCAAATGACTGAAAGTTCATTTCCAGTACCCATGACTCCACCTCCAAGAGTTGATCCTCCTCCAATGAATCACTCTGAATTTGTAGCTGCTTATCCACATCTTGCAACAGCTACAAATGTGAATTACAGGTTTACAGGAGACTCAAGCACCTCCAGACAAGACTTATCATCTGGTTCAAACAATATTCTGCCAAGAAGAACTACTCATTTCACAGACCATCGAAGGTGCTTTGAAACTGGTCAATCCTCAAGACAAAATGAAAACAGAGAACCAAGAAGACCTACAGAAAGAATGTTGCCTCCATCTCACTTTGATCATGTTCAACGCGCTGGAGGGATTCTGAAACCTCCAAAAAAACGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

367

Amino Acids

42.42

Weight (kDa)

8.89

Isoelectric Point (pI)

54.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 33 - 110 3.3e-17 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 172 - 220 6.3e-22 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 319
Acc36I ACCTGC 1 cut(s) 319
AccII CGCG 1 cut(s) 1068
AclWI GGATC 3 cut(s) 47, 60, 788
AcsI RAATTY 2 cut(s) 730, 818
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 1 cut(s) 768
AflII CTTAAG 1 cut(s) 109
AgsI TTSAA 9 cut(s) 95, 102, 146, 298, 545, 716, 918, 974, 1064
AluBI AGCT 8 cut(s) 80, 98, 341, 431, 621, 647, 827, 851
AluI AGCT 8 cut(s) 80, 98, 341, 431, 621, 647, 827, 851
Alw26I GTCTC 5 cut(s) 118, 407, 605, 744, 873
AlwI GGATC 3 cut(s) 47, 60, 788
AlwNI CAGNNNCTG 1 cut(s) 621
ApeKI GCWGC 2 cut(s) 314, 827
ApoI RAATTY 2 cut(s) 730, 818
AspLEI GCGC 1 cut(s) 1070
AsuHPI GGTGA 1 cut(s) 578
BamHI GGATCC 1 cut(s) 52
BanII GRGCYC 1 cut(s) 697
BbsI GAAGAC 2 cut(s) 613, 1024
BbvI GCAGC 2 cut(s) 326, 814
BccI CCATC 5 cut(s) 82, 262, 318, 966, 1051
BcgI CGANNNNNNTGC 4 cut(s) 184, 218, 594, 628
BclI TGATCA 1 cut(s) 1054
BcoDI GTCTC 5 cut(s) 118, 407, 605, 744, 873
BfaI CTAG 3 cut(s) 35, 440, 458
BfmI CTRYAG 2 cut(s) 613, 1023
BfrI CTTAAG 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 319
BglII AGATCT 1 cut(s) 436
BisI GCNGC 2 cut(s) 315, 828
BlsI GCNGC 2 cut(s) 316, 829
BmiI GGNNCC 4 cut(s) 54, 285, 336, 587
BmrI ACTGGG 1 cut(s) 730
BmsI GCATC 2 cut(s) 211, 687
BmuI ACTGGG 1 cut(s) 730
BpiI GAAGAC 2 cut(s) 613, 1024
BplI GAGNNNNNCTC 2 cut(s) 461, 493
BpmI CTGGAG 3 cut(s) 321, 877, 1092
BpuEI CTTGAG 3 cut(s) 417, 868, 973
BsaI GGTCTC 1 cut(s) 118
BsaJI CCNNGG 1 cut(s) 287
BsaXI ACNNNNNCTCC 2 cut(s) 766, 796
Bse1I ACTGG 4 cut(s) 235, 736, 764, 982
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 1 cut(s) 25
BseMII CTCAG 4 cut(s) 212, 497, 524, 688
BseNI ACTGG 4 cut(s) 235, 736, 764, 982
BseRI GAGGAG 2 cut(s) 471, 789
BseXI GCAGC 2 cut(s) 326, 814
BsgI GTGCAG 1 cut(s) 395
Bsh1236I CGCG 1 cut(s) 1068
BslFI GGGAC 1 cut(s) 201
BsmAI GTCTC 5 cut(s) 118, 407, 605, 744, 873
BsmBI CGTCTC 2 cut(s) 407, 605
BsmFI GGGAC 1 cut(s) 201
Bso31I GGTCTC 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 697
Bsp143I GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
BspCNI CTCAG 4 cut(s) 211, 496, 525, 687
BspFNI CGCG 1 cut(s) 1068
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 4 cut(s) 54, 285, 336, 587
BspMAI CTGCAG 1 cut(s) 617
BspMI ACCTGC 1 cut(s) 319
BspPI GGATC 3 cut(s) 47, 60, 788
BspTI CTTAAG 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 118
BsrI ACTGG 4 cut(s) 235, 736, 764, 982
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
Bst4CI ACNGT 3 cut(s) 288, 418, 653
Bst6I CTCTTC 2 cut(s) 205, 405
BstAFI CTTAAG 1 cut(s) 109
BstDEI CTNAG 4 cut(s) 198, 483, 533, 674
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 1068
BstHHI GCGC 1 cut(s) 1070
BstKTI GATC 9 cut(s) 55, 182, 262, 367, 439, 450, 722, 796, 1057
BstMAI GTCTC 5 cut(s) 118, 407, 605, 744, 873
BstMBI GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
BstSFI CTRYAG 2 cut(s) 613, 1023
BstUI CGCG 1 cut(s) 1068
BstV1I GCAGC 2 cut(s) 326, 814
BstV2I GAAGAC 2 cut(s) 613, 1024
BstX2I RGATCY 2 cut(s) 52, 436
BstXI CCANNNNNNTGG 1 cut(s) 183
BstYI RGATCY 2 cut(s) 52, 436
BtgI CCRYGG 1 cut(s) 287
BtgZI GCGATG 1 cut(s) 664
BtsCI GGATG 1 cut(s) 25
BveI ACCTGC 1 cut(s) 319
CaiI CAGNNNCTG 1 cut(s) 621
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 1070
Csp6I GTAC 1 cut(s) 767
CviAII CATG 6 cut(s) 74, 183, 206, 467, 772, 1058
CviQI GTAC 1 cut(s) 767
DdeI CTNAG 4 cut(s) 198, 483, 533, 674
DpnI GATC 9 cut(s) 54, 181, 261, 366, 438, 449, 721, 795, 1056
DpnII GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
Eam1104I CTCTTC 2 cut(s) 205, 405
EarI CTCTTC 2 cut(s) 205, 405
Eco24I GRGCYC 1 cut(s) 697
Eco31I GGTCTC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 730
EcoT38I GRGCYC 1 cut(s) 697
Esp3I CGTCTC 2 cut(s) 407, 605
FaeI CATG 6 cut(s) 77, 186, 209, 470, 775, 1061
FaqI GGGAC 1 cut(s) 201
FatI CATG 6 cut(s) 73, 182, 205, 466, 771, 1057
FbaI TGATCA 1 cut(s) 1054
Fnu4HI GCNGC 2 cut(s) 315, 828
FokI GGATG 1 cut(s) 32
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 2 cut(s) 315, 828
FspBI CTAG 3 cut(s) 35, 440, 458
GlaI GCGC 1 cut(s) 1069
GluI GCNGC 2 cut(s) 315, 828
GsuI CTGGAG 3 cut(s) 321, 877, 1092
HhaI GCGC 1 cut(s) 1070
Hin1II CATG 6 cut(s) 77, 186, 209, 470, 775, 1061
Hin6I GCGC 1 cut(s) 1068
HinP1I GCGC 1 cut(s) 1068
HincII GTYRAC 1 cut(s) 665
HindII GTYRAC 1 cut(s) 665
HindIII AAGCTT 2 cut(s) 78, 96
HinfI GANTC 8 cut(s) 196, 549, 560, 609, 775, 808, 881, 1078
HphI GGTGA 1 cut(s) 578
Hpy166II GTNNAC 4 cut(s) 633, 665, 671, 873
Hpy188I TCNGA 8 cut(s) 117, 132, 264, 409, 486, 605, 817, 1083
Hpy188III TCNNGA 6 cut(s) 35, 218, 434, 467, 894, 990
Hpy8I GTNNAC 4 cut(s) 633, 665, 671, 873
HpyAV CCTTC 2 cut(s) 273, 957
HpyCH4III ACNGT 3 cut(s) 288, 418, 653
HpyCH4IV ACGT 3 cut(s) 227, 598, 667
HpyCH4V TGCA 8 cut(s) 205, 224, 248, 274, 376, 575, 615, 845
HpyF3I CTNAG 4 cut(s) 198, 483, 533, 674
HpySE526I ACGT 3 cut(s) 227, 598, 667
Hsp92II CATG 6 cut(s) 77, 186, 209, 470, 775, 1061
HspAI GCGC 1 cut(s) 1068
Ksp22I TGATCA 1 cut(s) 1054
Kzo9I GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 2 cut(s) 326, 814
LweI GCATC 2 cut(s) 211, 687
MaeI CTAG 3 cut(s) 35, 440, 458
MaeII ACGT 3 cut(s) 227, 598, 667
MaeIII GTNAC 1 cut(s) 352
MalI GATC 9 cut(s) 54, 181, 261, 366, 438, 449, 721, 795, 1056
MboI GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
MboII GAAGA 6 cut(s) 222, 422, 547, 618, 948, 1029
MfeI CAATTG 1 cut(s) 301
MflI RGATCY 2 cut(s) 52, 436
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 6 cut(s) 38, 255, 301, 730, 818, 862
MlyI GAGTC 4 cut(s) 190, 603, 769, 875
MseI TTAA 2 cut(s) 110, 153
MspCI CTTAAG 1 cut(s) 109
MunI CAATTG 1 cut(s) 301
MvnI CGCG 1 cut(s) 1068
NdeII GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
NlaIII CATG 6 cut(s) 77, 186, 209, 470, 775, 1061
NlaIV GGNNCC 4 cut(s) 54, 285, 336, 587
NmuCI GTSAC 1 cut(s) 352
PagI TCATGA 1 cut(s) 466
PaqCI CACCTGC 1 cut(s) 319
PfeI GAWTC 4 cut(s) 549, 560, 808, 1078
PkrI GCNGC 2 cut(s) 316, 829
PleI GAGTC 4 cut(s) 190, 603, 769, 875
PpsI GAGTC 4 cut(s) 190, 603, 769, 875
PspN4I GGNNCC 4 cut(s) 54, 285, 336, 587
PsrI GAACNNNNNNTAC 2 cut(s) 221, 253
PstI CTGCAG 1 cut(s) 617
PstNI CAGNNNCTG 1 cut(s) 621
PsuI RGATCY 2 cut(s) 52, 436
RsaI GTAC 1 cut(s) 768
RsaNI GTAC 1 cut(s) 767
SaqAI TTAA 2 cut(s) 110, 153
SatI GCNGC 2 cut(s) 315, 828
Sau3AI GATC 9 cut(s) 52, 179, 259, 364, 436, 447, 719, 793, 1054
SchI GAGTC 4 cut(s) 190, 603, 769, 875
SduI GDGCHC 1 cut(s) 697
SfaNI GCATC 2 cut(s) 211, 687
SfcI CTRYAG 2 cut(s) 613, 1023
SmlI CTYRAG 4 cut(s) 109, 432, 883, 988
SmoI CTYRAG 4 cut(s) 109, 432, 883, 988
Sse9I AATT 6 cut(s) 38, 255, 301, 730, 818, 862
SspI AATATT 1 cut(s) 925
SspMI CTAG 3 cut(s) 35, 440, 458
TaaI ACNGT 3 cut(s) 288, 418, 653
TaiI ACGT 3 cut(s) 230, 601, 670
TaqI TCGA 4 cut(s) 322, 558, 643, 961
TasI AATT 6 cut(s) 38, 255, 301, 730, 818, 862
TfiI GAWTC 4 cut(s) 549, 560, 808, 1078
Tru1I TTAA 2 cut(s) 110, 153
Tru9I TTAA 2 cut(s) 110, 153
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 314, 827
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 8 cut(s) 55, 90, 267, 455, 743, 747, 821, 1014
Vha464I CTTAAG 1 cut(s) 109
XapI RAATTY 2 cut(s) 730, 818
XbaI TCTAGA 1 cut(s) 34
XspI CTAG 3 cut(s) 35, 440, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.