AT2G13500

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005646
Physical Location & Seq
Forward (+)
5627936 .. 5629099
1164 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G13500.1

Sequence Viewer

Length: 1017 bp
ATGGTCGATGTGTTATGGGACGAGATTCAAAATCTGGAGTTGGGTCAAGATTATCCTACTTTGTTCATTCCCAATGAGGCCTATGTGATGGTTGAAGACCGCAACAGGCTTAGTCTGATTGCCAGACCTCTGCTTCCAAGGGTACAAAATTTTAACGCTGTTATTAATGCTTTACCAAGAGCTTGGGGTCTTACTGCTCATGTTCATGGACGAATCCTAGATGCTACGTATGTTCAACTTCTGTTTCAAACAGAGATATACTTGTTATCGGTTCAAAGGAGGGAAGTATGGTTGTTTAATAACTGGTTTGTTGCATCTCAAAGATGGGAGGCTGCTCCTTCTTTGAATTTTGTAACCACAATTGATCTTTGGGTTCAAATGCGTGGCATACCAATTCTTTATGTTTTTGAAAAAACAGAGCTAGAGATTGCTCAAGAGCTTGGGGAGATTATATCTTTGGACTTTCATGATGCCACTTCTACACAGATTGCTTATATCCGAGTTAGAGTTCGTTTTGGTATTACTGATCGTTTAAGGTTCTTTCAGAGAATCACCTTTGAGTCAGGAGAAACGGCTTTAATTCGGTTTCAGTATGAGAGACTTAGGCGCATCTATAGTTGTTTTAGGTTTACCCATAACTTGAACTATTATCCTTATCGCCAACGTTTTCAGAGCATTGACAGGGAAAGAGCTGTTTTCAGAGATAGTGTTTTGAGGTCAAGCTTTAACTCTCAATCTCAGATGACTGATAGCTCTCTTCAGGTTCCTTTACATCCACCTCCACGAGTTCCATCCTCTGGAGATGCGATTACTTCTGGAATAATGCGAGTCTTTGAGGTGGGTGAATCATCAAGAGGTGTTCAAGATGGGGAATCTCACAATAACATGAATCTTGGGGAGTCATCTAAGAGGAAGAACTTGGTCATTCTTCAGCGTGATAAGGAGTCAAGACAACACAAACAACAAGAAACTGAGCAAAGAATGGGAGGTATTCTGAAACCTCCTAAGAAACGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

338

Amino Acids

39.63

Weight (kDa)

9.32

Isoelectric Point (pI)

49.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 32 - 111 2.2e-15 Domain of unknown function (DUF4283)
zf-CCHC_4 PF14392 172 - 219 3.4e-17 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 797
AciI CCGC 1 cut(s) 100
AclI AACGTT 2 cut(s) 664, 1012
AcsI RAATTY 2 cut(s) 148, 346
AcuI CTGAAG 2 cut(s) 743, 914
AfaI GTAC 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 797
AluBI AGCT 6 cut(s) 182, 421, 439, 692, 723, 753
AluI AGCT 6 cut(s) 182, 421, 439, 692, 723, 753
Alw26I GTCTC 1 cut(s) 592
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 2 cut(s) 148, 346
AseI ATTAAT 1 cut(s) 165
AspLEI GCGC 1 cut(s) 609
AsuHPI GGTGA 2 cut(s) 544, 854
BauI CACGAG 1 cut(s) 783
BbsI GAAGAC 1 cut(s) 102
BbvI GCAGC 1 cut(s) 319
BccI CCATC 4 cut(s) 82, 318, 799, 860
BceAI ACGGC 1 cut(s) 588
BcoDI GTCTC 1 cut(s) 592
BfaI CTAG 2 cut(s) 218, 422
BfmI CTRYAG 1 cut(s) 613
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
BmiI GGNNCC 1 cut(s) 765
BmsI GCATC 5 cut(s) 211, 323, 460, 618, 793
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 2 cut(s) 56, 819
BpuEI CTTGAG 1 cut(s) 417
BsaAI YACGTR 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 137
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse1I ACTGG 1 cut(s) 308
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 2 cut(s) 772, 791
BseLI CCNNNNNNNGG 1 cut(s) 797
BseMII CTCAG 2 cut(s) 752, 963
BseNI ACTGG 1 cut(s) 308
BseXI GCAGC 1 cut(s) 319
BshFI GGCC 1 cut(s) 80
BslFI GGGAC 1 cut(s) 32
BslI CCNNNNNNNGG 1 cut(s) 797
BsmAI GTCTC 1 cut(s) 592
BsmFI GGGAC 1 cut(s) 32
BsnI GGCC 1 cut(s) 80
Bsp143I GATC 2 cut(s) 364, 526
BspACI CCGC 1 cut(s) 100
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 751, 964
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 1 cut(s) 765
BsrI ACTGG 1 cut(s) 308
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 2 cut(s) 364, 526
BssSI CACGAG 1 cut(s) 783
BssT1I CCWWGG 1 cut(s) 137
Bst2BI CACGAG 1 cut(s) 783
Bst6I CTCTTC 1 cut(s) 762
BstBAI YACGTR 1 cut(s) 228
BstDEI CTNAG 6 cut(s) 110, 602, 738, 906, 972, 1005
BstF5I GGATG 2 cut(s) 772, 791
BstHHI GCGC 1 cut(s) 609
BstKTI GATC 2 cut(s) 367, 529
BstMAI GTCTC 1 cut(s) 592
BstMBI GATC 2 cut(s) 364, 526
BstSFI CTRYAG 1 cut(s) 613
BstSNI TACGTA 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 319
BstV2I GAAGAC 1 cut(s) 102
BstXI CCANNNNNNTGG 1 cut(s) 183
BsuRI GGCC 1 cut(s) 80
BtsCI GGATG 2 cut(s) 772, 791
CciI TCATGA 1 cut(s) 466
CfoI GCGC 1 cut(s) 609
Csp6I GTAC 1 cut(s) 143
CviAII CATG 4 cut(s) 200, 206, 467, 886
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 6 cut(s) 110, 602, 738, 906, 972, 1005
DpnI GATC 2 cut(s) 366, 528
DpnII GATC 2 cut(s) 364, 526
Eam1104I CTCTTC 1 cut(s) 762
EarI CTCTTC 1 cut(s) 762
Eco105I TACGTA 1 cut(s) 228
Eco130I CCWWGG 1 cut(s) 137
Eco147I AGGCCT 1 cut(s) 80
Eco57I CTGAAG 2 cut(s) 743, 914
EcoT14I CCWWGG 1 cut(s) 137
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 4 cut(s) 203, 209, 470, 889
FaqI GGGAC 1 cut(s) 32
FatI CATG 4 cut(s) 199, 205, 466, 885
Fnu4HI GCNGC 1 cut(s) 333
FokI GGATG 2 cut(s) 759, 778
Fsp4HI GCNGC 1 cut(s) 333
FspBI CTAG 2 cut(s) 218, 422
GlaI GCGC 1 cut(s) 608
GluI GCNGC 1 cut(s) 333
GsuI CTGGAG 2 cut(s) 56, 819
HaeIII GGCC 1 cut(s) 80
HhaI GCGC 1 cut(s) 609
Hin1II CATG 4 cut(s) 203, 209, 470, 889
Hin6I GCGC 1 cut(s) 607
HinP1I GCGC 1 cut(s) 607
HindIII AAGCTT 1 cut(s) 721
HphI GGTGA 2 cut(s) 544, 854
Hpy166II GTNNAC 1 cut(s) 630
Hpy188I TCNGA 7 cut(s) 117, 500, 546, 672, 701, 741, 996
Hpy8I GTNNAC 1 cut(s) 630
HpyAV CCTTC 1 cut(s) 348
HpyCH4IV ACGT 3 cut(s) 227, 664, 1012
HpyCH4V TGCA 1 cut(s) 314
HpyF3I CTNAG 6 cut(s) 110, 602, 738, 906, 972, 1005
HpySE526I ACGT 3 cut(s) 227, 664, 1012
Hsp92II CATG 4 cut(s) 203, 209, 470, 889
HspAI GCGC 1 cut(s) 607
Kzo9I GATC 2 cut(s) 364, 526
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 9 cut(s) 20, 91, 136, 289, 549, 667, 746, 783, 801
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 5 cut(s) 211, 323, 460, 618, 793
MaeI CTAG 2 cut(s) 218, 422
MaeII ACGT 3 cut(s) 227, 664, 1012
MaeIII GTNAC 1 cut(s) 352
MalI GATC 2 cut(s) 366, 528
MboI GATC 2 cut(s) 364, 526
MboII GAAGA 4 cut(s) 107, 749, 920, 925
MfeI CAATTG 1 cut(s) 360
MluCI AATT 5 cut(s) 148, 346, 360, 393, 579
MlyI GAGTC 4 cut(s) 569, 837, 908, 953
MseI TTAA 7 cut(s) 153, 165, 297, 533, 578, 726, 1015
MslI CAYNNNNRTG 1 cut(s) 204
MunI CAATTG 1 cut(s) 360
NdeII GATC 2 cut(s) 364, 526
NlaIII CATG 4 cut(s) 203, 209, 470, 889
NlaIV GGNNCC 1 cut(s) 765
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 80
PfeI GAWTC 6 cut(s) 25, 213, 549, 845, 872, 889
PflMI CCANNNNNTGG 1 cut(s) 797
PkrI GCNGC 1 cut(s) 334
PleI GAGTC 4 cut(s) 568, 836, 907, 952
PpsI GAGTC 4 cut(s) 568, 836, 907, 952
Ppu21I YACGTR 1 cut(s) 228
PshBI ATTAAT 1 cut(s) 165
Psp1406I AACGTT 2 cut(s) 664, 1012
PspN4I GGNNCC 1 cut(s) 765
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
RseI CAYNNNNRTG 1 cut(s) 204
SaqAI TTAA 7 cut(s) 153, 165, 297, 533, 578, 726, 1015
SatI GCNGC 1 cut(s) 333
Sau3AI GATC 2 cut(s) 364, 526
SchI GAGTC 4 cut(s) 569, 837, 908, 953
SfaNI GCATC 5 cut(s) 211, 323, 460, 618, 793
SfcI CTRYAG 1 cut(s) 613
SmiMI CAYNNNNRTG 1 cut(s) 204
SmlI CTYRAG 1 cut(s) 432
SmoI CTYRAG 1 cut(s) 432
SnaBI TACGTA 1 cut(s) 228
Sse9I AATT 5 cut(s) 148, 346, 360, 393, 579
SseBI AGGCCT 1 cut(s) 80
SsiI CCGC 1 cut(s) 100
SspMI CTAG 2 cut(s) 218, 422
StuI AGGCCT 1 cut(s) 80
StyI CCWWGG 1 cut(s) 137
TaiI ACGT 3 cut(s) 230, 667, 1015
TaqI TCGA 1 cut(s) 6
TasI AATT 5 cut(s) 148, 346, 360, 393, 579
TfiI GAWTC 6 cut(s) 25, 213, 549, 845, 872, 889
Tru1I TTAA 7 cut(s) 153, 165, 297, 533, 578, 726, 1015
Tru9I TTAA 7 cut(s) 153, 165, 297, 533, 578, 726, 1015
TseI GCWGC 1 cut(s) 332
TspDTI ATGAA 4 cut(s) 55, 194, 455, 902
Van91I CCANNNNNTGG 1 cut(s) 797
VspI ATTAAT 1 cut(s) 165
XapI RAATTY 2 cut(s) 148, 346
XspI CTAG 2 cut(s) 218, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.