AT4G02000

At4g02000-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0005646
Physical Location & Seq
Forward (+)
874880 .. 876181
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G02000.1

Sequence Viewer

Length: 945 bp
ATGACATCTCTAACGATTGATCTCCTGTCAGTACTACGAAGGGAACCTTGGTTGTATAACAACTGGTTTGTGACAACTCATAGATGGGAGGTAAATCTTACGTTTCACCTGTTAACATCTATTGAATTATGGGTTCAGATGCGTGGAATTCCTCTTTTGTATGTGTGTGAGGAAACTGCTTTAGAGATAGCACATGAGTTAGGAAAGATTCTCACTCTGGATTTTCATGACTCCACCACGACTCAAATAGCTTATATCCGTGTGAGAATCCGGTTTGGAATTACAGATCGCCTGAGGTTTTTCCAAAGAATCATTTTTGATTTTGGTGAGGCTGCTCTGATAAGCTTTCAGTATGAAAGACTTAGGAGAATCTGTAGTAGCTGCTTCAGAATGACTCATCACAGGAACTCATGTCCTTACCGTCAGATTGAGCCTCTCCATAGGGTGACTAACTCCACTGCTCAAAGGAATGTTAGAGAAGAGGTGTTCATGAGAGATGAAAATCTGAGATCTTCAATGAATTCACAATCTCAGATGTCTGAGAGTTCGTTTCCTACTCCTATCGATCCACCTCCAAGGATTCCTCATCCTCCTTTAAATCCTGATGAATTGGTGGCTGCATATTACCCTCATACAAGAGCTACTTCTTTACCAAACTTTGCAGGTCCTTTGCCTCAGGTACCTCTTAGAAAAAATGTGGATGAAAGGGATTCTAACATCCAGCCTTTCTCCGGACCTGCATTTGCAGCACACTCTCCACGCCTAGTGGAAGTAGGTGAATCATCAAGGCAAGGTGAGAATACTCAGAATGTTCACACGGTGGAAAAGGGGGATTCATCAAAAAGAAAGAACATGGGTGGTCCCAGGTTTAAAGATGATGCAAGAAAGTCCAATGAAGATGAGCATATGAATGGAGGGATTCTGAAACCTCCAAAAAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

314

Amino Acids

36.36

Weight (kDa)

9.08

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC_4 PF14392 92 - 140 8.1e-20 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 653, 745
Acc65I GGTACC 1 cut(s) 679
AccB1I GGYRCC 1 cut(s) 679
AccIII TCCGGA 1 cut(s) 731
AclWI GGATC 1 cut(s) 560
AcsI RAATTY 2 cut(s) 147, 520
AcuI CTGAAG 1 cut(s) 370
AdeI CACNNNGTG 1 cut(s) 820
AfaI GTAC 2 cut(s) 33, 681
AfiI CCNNNNNNNGG 1 cut(s) 731
AgsI TTSAA 2 cut(s) 125, 516
AjnI CCWGG 1 cut(s) 863
AjuI GAANNNNNNNTTGG 2 cut(s) 31, 63
AluBI AGCT 4 cut(s) 251, 345, 381, 641
AluI AGCT 4 cut(s) 251, 345, 381, 641
AlwI GGATC 1 cut(s) 560
Aor13HI TCCGGA 1 cut(s) 731
ApeKI GCWGC 4 cut(s) 332, 381, 617, 746
ApoI RAATTY 2 cut(s) 147, 520
Asp718I GGTACC 1 cut(s) 679
AspS9I GGNCC 3 cut(s) 665, 734, 860
AsuHPI GGTGA 5 cut(s) 98, 338, 457, 788, 806
AvaII GGWCC 3 cut(s) 665, 734, 860
AxyI CCTNAGG 2 cut(s) 293, 675
BanI GGYRCC 1 cut(s) 679
BbvI GCAGC 4 cut(s) 319, 368, 604, 758
BccI CCATC 1 cut(s) 78
BciT130I CCWGG 1 cut(s) 865
BfaI CTAG 1 cut(s) 764
BfmI CTRYAG 1 cut(s) 373
BfuAI ACCTGC 2 cut(s) 653, 745
BglII AGATCT 1 cut(s) 509
BisI GCNGC 4 cut(s) 333, 382, 618, 747
BlsI GCNGC 4 cut(s) 334, 383, 619, 748
BmcAI AGTACT 1 cut(s) 33
Bme1390I CCNGG 1 cut(s) 865
Bme18I GGWCC 3 cut(s) 665, 734, 860
BmgT120I GGNCC 3 cut(s) 665, 734, 860
BmiI GGNNCC 3 cut(s) 45, 681, 862
BmrFI CCNGG 1 cut(s) 865
BmsI GCATC 2 cut(s) 129, 868
Bsa29I ATCGAT 1 cut(s) 564
BsaBI GATNNNNATC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 47, 575, 863
BsaWI WCCGGW 2 cut(s) 270, 731
BsaXI ACNNNNNCTCC 2 cut(s) 358, 388
Bsc4I CCNNNNNNNGG 1 cut(s) 731
Bse1I ACTGG 1 cut(s) 68
Bse21I CCTNAGG 2 cut(s) 293, 675
Bse8I GATNNNNATC 1 cut(s) 501
BseAI TCCGGA 1 cut(s) 731
BseBI CCWGG 1 cut(s) 865
BseCI ATCGAT 1 cut(s) 564
BseDI CCNNGG 3 cut(s) 47, 575, 863
BseGI GGATG 3 cut(s) 586, 706, 717
BseJI GATNNNNATC 1 cut(s) 501
BseLI CCNNNNNNNGG 1 cut(s) 731
BseMII CTCAG 6 cut(s) 284, 497, 531, 545, 689, 818
BseNI ACTGG 1 cut(s) 68
BseXI GCAGC 4 cut(s) 319, 368, 604, 758
BshNI GGYRCC 1 cut(s) 679
BshVI ATCGAT 1 cut(s) 564
BsiSI CCGG 2 cut(s) 271, 732
BslFI GGGAC 1 cut(s) 846
BslI CCNNNNNNNGG 1 cut(s) 731
BsmFI GGGAC 1 cut(s) 846
Bsp13I TCCGGA 1 cut(s) 731
Bsp143I GATC 4 cut(s) 19, 286, 509, 565
BspCNI CTCAG 6 cut(s) 285, 498, 532, 544, 688, 817
BspDI ATCGAT 1 cut(s) 564
BspEI TCCGGA 1 cut(s) 731
BspHI TCATGA 2 cut(s) 226, 489
BspLI GGNNCC 3 cut(s) 45, 681, 862
BspMI ACCTGC 2 cut(s) 653, 745
BspPI GGATC 1 cut(s) 560
BspT107I GGYRCC 1 cut(s) 679
BsrI ACTGG 1 cut(s) 68
BssECI CCNNGG 3 cut(s) 47, 575, 863
BssMI GATC 4 cut(s) 19, 286, 509, 565
BssT1I CCWWGG 2 cut(s) 47, 575
Bst2UI CCWGG 1 cut(s) 865
Bst4CI ACNGT 2 cut(s) 422, 820
Bst6I CTCTTC 1 cut(s) 474
BstDEI CTNAG 8 cut(s) 293, 362, 506, 531, 540, 675, 686, 804
BstF5I GGATG 3 cut(s) 586, 706, 717
BstKTI GATC 4 cut(s) 22, 289, 512, 568
BstMBI GATC 4 cut(s) 19, 286, 509, 565
BstMWI GCNNNNNNNGC 1 cut(s) 746
BstNI CCWGG 1 cut(s) 865
BstSCI CCNGG 1 cut(s) 863
BstSFI CTRYAG 1 cut(s) 373
BstV1I GCAGC 4 cut(s) 319, 368, 604, 758
BstX2I RGATCY 1 cut(s) 509
BstYI RGATCY 1 cut(s) 509
Bsu15I ATCGAT 1 cut(s) 564
Bsu36I CCTNAGG 2 cut(s) 293, 675
BsuTUI ATCGAT 1 cut(s) 564
BtsCI GGATG 3 cut(s) 586, 706, 717
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 456
BveI ACCTGC 2 cut(s) 653, 745
CciI TCATGA 2 cut(s) 226, 489
Cfr13I GGNCC 3 cut(s) 665, 734, 860
ClaI ATCGAT 1 cut(s) 564
Csp6I GTAC 2 cut(s) 32, 680
CviAII CATG 5 cut(s) 194, 227, 411, 490, 853
CviJI RGCY 8 cut(s) 251, 332, 345, 381, 433, 617, 641, 724
CviKI_1 RGCY 8 cut(s) 251, 332, 345, 381, 433, 617, 641, 724
CviQI GTAC 2 cut(s) 32, 680
DdeI CTNAG 8 cut(s) 293, 362, 506, 531, 540, 675, 686, 804
DpnI GATC 4 cut(s) 21, 288, 511, 567
DpnII GATC 4 cut(s) 19, 286, 509, 565
DraI TTTAAA 2 cut(s) 597, 871
DraIII CACNNNGTG 1 cut(s) 820
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Eco130I CCWWGG 2 cut(s) 47, 575
Eco47I GGWCC 3 cut(s) 665, 734, 860
Eco57I CTGAAG 1 cut(s) 370
Eco81I CCTNAGG 2 cut(s) 293, 675
EcoO109I RGGNCCY 1 cut(s) 665
EcoRI GAATTC 2 cut(s) 147, 520
EcoRII CCWGG 1 cut(s) 863
EcoT14I CCWWGG 2 cut(s) 47, 575
ErhI CCWWGG 2 cut(s) 47, 575
FaeI CATG 5 cut(s) 197, 230, 414, 493, 856
FalI AAGNNNNNCTT 4 cut(s) 31, 63, 628, 660
FaqI GGGAC 1 cut(s) 846
FatI CATG 5 cut(s) 193, 226, 410, 489, 852
FauNDI CATATG 1 cut(s) 906
Fnu4HI GCNGC 4 cut(s) 333, 382, 618, 747
FokI GGATG 3 cut(s) 573, 704, 713
Fsp4HI GCNGC 4 cut(s) 333, 382, 618, 747
FspBI CTAG 1 cut(s) 764
GluI GCNGC 4 cut(s) 333, 382, 618, 747
HapII CCGG 2 cut(s) 271, 732
Hin1II CATG 5 cut(s) 197, 230, 414, 493, 856
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 343
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 2 cut(s) 271, 732
HphI GGTGA 5 cut(s) 98, 338, 457, 788, 806
Hpy166II GTNNAC 2 cut(s) 114, 814
Hpy188I TCNGA 9 cut(s) 138, 339, 389, 426, 507, 534, 541, 807, 924
Hpy188III TCNNGA 5 cut(s) 218, 227, 490, 602, 732
Hpy8I GTNNAC 2 cut(s) 114, 814
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 2 cut(s) 422, 820
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 5 cut(s) 620, 662, 740, 746, 881
HpyF10VI GCNNNNNNNGC 1 cut(s) 746
HpyF3I CTNAG 8 cut(s) 293, 362, 506, 531, 540, 675, 686, 804
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 5 cut(s) 197, 230, 414, 493, 856
Kpn2I TCCGGA 1 cut(s) 731
KpnI GGTACC 1 cut(s) 683
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 4 cut(s) 19, 286, 509, 565
Lsp1109I GCAGC 4 cut(s) 319, 368, 604, 758
LweI GCATC 2 cut(s) 129, 868
MaeI CTAG 1 cut(s) 764
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 2 cut(s) 70, 445
MalI GATC 4 cut(s) 21, 288, 511, 567
MboI GATC 4 cut(s) 19, 286, 509, 565
MboII GAAGA 3 cut(s) 491, 504, 908
MflI RGATCY 1 cut(s) 509
MluCI AATT 5 cut(s) 125, 147, 279, 520, 608
MlyI GAGTC 3 cut(s) 224, 235, 388
MroI TCCGGA 1 cut(s) 731
MseI TTAA 3 cut(s) 113, 596, 870
MslI CAYNNNNRTG 1 cut(s) 909
MspI CCGG 2 cut(s) 271, 732
MspR9I CCNGG 1 cut(s) 865
MvaI CCWGG 1 cut(s) 865
MwoI GCNNNNNNNGC 1 cut(s) 746
NdeI CATATG 1 cut(s) 906
NdeII GATC 4 cut(s) 19, 286, 509, 565
NlaIII CATG 5 cut(s) 197, 230, 414, 493, 856
NlaIV GGNNCC 3 cut(s) 45, 681, 862
NmuCI GTSAC 2 cut(s) 70, 445
PagI TCATGA 2 cut(s) 226, 489
PfeI GAWTC 9 cut(s) 208, 267, 309, 369, 580, 710, 779, 833, 919
PkrI GCNGC 4 cut(s) 334, 383, 619, 748
PleI GAGTC 3 cut(s) 224, 235, 388
PpsI GAGTC 3 cut(s) 224, 235, 388
PpuMI RGGWCCY 1 cut(s) 665
Psp5II RGGWCCY 1 cut(s) 665
Psp6I CCWGG 1 cut(s) 863
PspGI CCWGG 1 cut(s) 863
PspN4I GGNNCC 3 cut(s) 45, 681, 862
PspPI GGNCC 3 cut(s) 665, 734, 860
PspPPI RGGWCCY 1 cut(s) 665
PsuI RGATCY 1 cut(s) 509
RsaI GTAC 2 cut(s) 33, 681
RsaNI GTAC 2 cut(s) 32, 680
RseI CAYNNNNRTG 1 cut(s) 909
SaqAI TTAA 3 cut(s) 113, 596, 870
SatI GCNGC 4 cut(s) 333, 382, 618, 747
Sau3AI GATC 4 cut(s) 19, 286, 509, 565
Sau96I GGNCC 3 cut(s) 665, 734, 860
ScaI AGTACT 1 cut(s) 33
SchI GAGTC 3 cut(s) 224, 235, 388
ScrFI CCNGG 1 cut(s) 865
SfaNI GCATC 2 cut(s) 129, 868
SfcI CTRYAG 1 cut(s) 373
SinI GGWCC 3 cut(s) 665, 734, 860
SmiMI CAYNNNNRTG 1 cut(s) 909
Sse9I AATT 5 cut(s) 125, 147, 279, 520, 608
SspMI CTAG 1 cut(s) 764
StyD4I CCNGG 1 cut(s) 863
StyI CCWWGG 2 cut(s) 47, 575
TaaI ACNGT 2 cut(s) 422, 820
TaiI ACGT 1 cut(s) 104
TaqI TCGA 1 cut(s) 564
TasI AATT 5 cut(s) 125, 147, 279, 520, 608
TatI WGTACW 1 cut(s) 31
TfiI GAWTC 9 cut(s) 208, 267, 309, 369, 580, 710, 779, 833, 919
Tru1I TTAA 3 cut(s) 113, 596, 870
Tru9I TTAA 3 cut(s) 113, 596, 870
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 2 cut(s) 70, 445
TseI GCWGC 4 cut(s) 332, 381, 617, 746
Tsp45I GTSAC 2 cut(s) 70, 445
TspGWI ACGGA 1 cut(s) 248
TspRI CASTG 1 cut(s) 463
VpaK11BI GGWCC 3 cut(s) 665, 734, 860
XapI RAATTY 2 cut(s) 147, 520
XspI CTAG 1 cut(s) 764
ZrmI AGTACT 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.