AT2G22300

CG-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001949
Physical Location & Seq
Forward (+)
9471219 .. 9476646
5428 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G22300.2

Sequence Viewer

Length: 3099 bp
ATGGCGGAAGCAAGACGATTCAGCCCAGTTCATGAATTAGATGTTGGACAAATACTCTCAGAAGCACGACATCGATGGCTTCGTCCTCCTGAAATCTGTGAAATTTTACAGAATTACCAAAGATTTCAAATTTCTACTGAGCCACCTACTACACCATCAAGTGGGTCTGTTTTTATGTTTGATCGAAAGGTGCTCAGATACTTCAGGAAAGACGGTCACAATTGGAGGAAGAAAAAAGATGGAAAGACTGTGAAGGAAGCTCATGAGAGGTTGAAGGCGGGAAGCGTTGATGTTCTACATTGTTACTATGCGCATGGACAGGACAATGAAAACTTTCAAAGACGCAGTTACTGGTTGCTTCAGGAAGAACTTTCCCACATAGTTTTTGTTCACTACCTCGAAGTTAAGGGTAGTAGAGTTTCTACTTCTTTTAACCGGATGCAAAGGACTGAAGACGCGGCTCGGTCTCCTCAAGAAACTGGGGACGCCTTGACCAGTGAACATGATGGTTATGCTTCTTGCAGCTTCAATCAAAATGATCATAGCAATCATTCACAAACTACTGACTCAGCAAGTGTCAATGGTTTTCACTCTCCAGAACTTGAAGATGCTGAATCAGCATACAATCAGCATGGAAGTTCCACAGCTTACTCTCATCAAGAACTTCAGCAGCCTGCAACAGGAGGAAACCTTACTGGTTTTGATCCTTACTATCAAATCTCTTTGACGCCCAGGGATAGTTATCAAAAAGAGCTTCGCACAATCCCCGTAACTGATTCTTCAATTATGGTAGACAAAAGCAAAACTATCAACAGTCCTGGAGTAACAAATGGGTTAAAAAACAGAAAATCCATTGATTCCCAAACCTGGGAAGAGATTCTGGGAAATTGTGGTTCTGGAGTTGAAGCCTTACCTTTGCAGCCTAACAGTGAGCATGAAGTGCTGGACCAAATACTCGAAAGCTCTTTTACAATGCAAGATTTTGCTAGTCTACAAGAGTCCATGGTCAAAAGCCAGAATCAGGAGTTAAATTCAGGACTTACATCTGATCGTACCGTGTGGTTCCAAGGACAAGATATGGAGCTAAATGCTATAAGCAATCTAGCTTCGAATGAAAAAGCTCCATATCTATCTACGATGAAACAACATCTGTTACACGGTGCATTGGGCGAAGAAGGTCTGAAAAAGATGGACAGTTTCAATCGCTGGATGAGCAAAGAACTTGGAGATGTTGGTGTTATAGCTGATGCAAACGAGTCCTTTACTCAATCAAGTTCAAGAACCTACTGGGAAGAAGTTGAGAGTGAGGATGGGTCCAATGGTCACAACTCTAGGAGGGACATGGATGGATATGTTATGAGTCCTTCCCTCTCAAAGGAACAGCTCTTTAGCATCAATGACTTCTCTCCAAGCTGGGCTTATGTGGGTTGTGAAGTGGTGGTTTTTGTCACTGGAAAATTCTTAAAGACTCGGGAGGAGACTGAAATTGGCGAGTGGTCTTGCATGTTTGGGCAAACAGAAGTTCCAGCAGATGTTATATCTAATGGTATTCTCCAATGTGTTGCTCCTATGCATGAGGCTGGAAGAGTTCCCTTTTATGTAACATGTTCCAACAGATTGGCATGCAGTGAAGTGCGTGAATTCGAGTATAAGGTTGCCGAGTCTCAAGTTTTTGATAGAGAAGCAGATGATGAGTCTACTATTGACATTCTTGAGGCAAGATTTGTTAAACTGTTGTGCTCGAAATCTGAAAATACAAGCCCTGTTTCTGGGAATGACAGTGATTTGTCACAATTGAGCGAGAAGATTAGTTTACTACTTTTCGAGAACGATGACCAGTTGGATCAGATGCTCATGAATGAAATCTCCCAAGAAAATATGAAAAACAACCTTTTGCAGGAATTTCTGAAAGAAAGCTTACACTCATGGCTTTTACAAAAGATAGCAGAAGGTGGAAAAGGTCCAAGTGTTTTGGATGAAGGTGGCCAAGGTGTATTACATTTTGCAGCTTCTCTTGGTTACAACTGGGCCTTAGAACCAACAATAATCGCTGGTGTAAGCGTTGATTTTCGCGATGTAAATGGTTGGACCGCACTTCATTGGGCAGCTTTCTTTGGCAGGGAGAGGATAATTGGTTCACTCATAGCTCTTGGTGCTGCTCCTGGAACTTTAACTGACCCAAATCCAGATTTCCCATCAGGAAGCACACCTTCTGATCTAGCCTATGCTAATGGTCACAAAGGAATTGCTGGTTATCTCTCAGAATATGCCTTAAGAGCTCATGTTTCTTTGCTCAGTCTAAATGATAAAAATGCCGAAACTGTTGAGATGGCTCCTAGCCCATCCAGCTCATCATTGACAGACTCGTTGACAGCTGTACGTAACGCTACTCAGGCAGCAGCTCGGATTCATCAGGTTTTCAGGGCTCAGTCTTTCCAGAAGAAGCAACTAAAAGAATTTGGAGATAAAAAGCTTGGAATGTCGGAGGAGCGTGCTCTTTCGATGCTTGCTCCTAAAACACACAAATCAGGAAGGGCACATAGTGATGATTCCGTGCAAGCCGCTGCTATTCGGATTCAGAACAAGTTCCGAGGTTACAAGGGAAGAAAAGACTATTTGATTACCAGACAAAGAATCATCAAAATACAGGCTCATGTGAGAGGTTATCAGTTTAGGAAAAACTACAGAAAGATAATTTGGTCAGTGGGGGTATTAGAGAAGGTGATATTACGTTGGAGACGGAAAGGAGCTGGTTTGCGCGGGTTTAAGTCAGAAGCACTTGTTGAGAAGATGCAAGACGGAACAGAGAAAGAAGAAGATGATGATTTTTTCAAACAAGGAAGAAAGCAAACAGAGGATAGGCTACAAAAAGCTCTTGCAAGAGTGAAATCCATGGTTCAATATCCTGAAGCTAGAGATCAATACCGTAGATTGCTAAATGTTGTCAACGACATCCAAGAAAGCAAGGTTGAAAAAGCTCTTGAAAATTCAGAAGCAACTTGTTTTGATGATGATGATGATCTGATAGATATTGAAGCATTGTTGGAAGATGATGACACATTGATGTTGCCTATGTCCTCATCTTTGTGGACCAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

1032

Amino Acids

116.11

Weight (kDa)

5.36

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 21 - 134 1.4e-50 CG-1 domain
Ank_2 PF12796 666 - 756 7.5e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 312
AccB7I CCANNNNNTGG 1 cut(s) 161
AccI GTMKAC 3 cut(s) 792, 991, 1697
AccII CGCG 3 cut(s) 458, 2073, 2760
AciI CCGC 6 cut(s) 5, 278, 458, 2091, 2562, 2760
AclWI GGATC 2 cut(s) 698, 1851
AcoI YGGCCR 1 cut(s) 1984
AcsI RAATTY 8 cut(s) 102, 129, 1030, 1457, 1640, 1901, 2456, 2986
AcuI CTGAAG 5 cut(s) 187, 344, 471, 650, 2928
AcyI GRCGYC 2 cut(s) 486, 728
AdeI CACNNNGTG 1 cut(s) 2543
AfaI GTAC 2 cut(s) 1054, 2379
AfiI CCNNNNNNNGG 8 cut(s) 161, 680, 867, 868, 1021, 1375, 1769, 1897
AflII CTTAAG 1 cut(s) 2272
AflIII ACRYGT 1 cut(s) 1604
AjnI CCWGG 4 cut(s) 731, 817, 866, 2161
AjuI GAANNNNNNNTTGG 4 cut(s) 27, 59, 2680, 2712
AloI GAACNNNNNNTCC 2 cut(s) 2119, 2151
Alw21I GWGCWC 4 cut(s) 195, 1742, 2281, 2497
Alw26I GTCTC 4 cut(s) 471, 1472, 1668, 2731
AlwI GGATC 2 cut(s) 698, 1851
AlwNI CAGNNNCTG 1 cut(s) 351
Ama87I CYCGRG 1 cut(s) 1470
AoxI GGCC 2 cut(s) 1984, 2028
ApeKI GCWGC 9 cut(s) 522, 670, 919, 2006, 2105, 2156, 2396, 2399, 2564
ApoI RAATTY 8 cut(s) 102, 129, 1030, 1457, 1640, 1901, 2456, 2986
Asp700I GAANNNNTTC 3 cut(s) 333, 876, 2585
AspLEI GCGC 2 cut(s) 313, 2760
AspS9I GGNCC 6 cut(s) 946, 1314, 1961, 2028, 2088, 3090
AsuHPI GGTGA 1 cut(s) 2734
AsuII TTCGAA 1 cut(s) 1109
AvaI CYCGRG 1 cut(s) 1470
AvaII GGWCC 5 cut(s) 946, 1314, 1961, 2088, 3090
BaeGI GKGCMC 1 cut(s) 2539
BalI TGGCCA 1 cut(s) 1986
BanII GRGCYC 2 cut(s) 2281, 2428
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 4 cut(s) 195, 1742, 2281, 2497
BbvI GCAGC 9 cut(s) 534, 682, 931, 2018, 2117, 2143, 2408, 2411, 2551
BciT130I CCWGG 4 cut(s) 733, 819, 868, 2163
BclI TGATCA 1 cut(s) 538
BcoDI GTCTC 4 cut(s) 471, 1472, 1668, 2731
BfaI CTAG 6 cut(s) 987, 1103, 1332, 2219, 2337, 2913
BfmI CTRYAG 1 cut(s) 2683
BfrI CTTAAG 1 cut(s) 2272
Bme1390I CCNGG 4 cut(s) 733, 819, 868, 2163
Bme18I GGWCC 5 cut(s) 946, 1314, 1961, 2088, 3090
BmeT110I CYCGRG 1 cut(s) 1470
BmgT120I GGNCC 6 cut(s) 946, 1314, 1961, 2028, 2088, 3090
BmiI GGNNCC 3 cut(s) 1064, 1315, 2334
BmrFI CCNGG 4 cut(s) 733, 819, 868, 2163
BmrI ACTGGG 4 cut(s) 20, 489, 1297, 2035
BmsI GCATC 7 cut(s) 429, 598, 1237, 1401, 1839, 2493, 2781
BmuI ACTGGG 4 cut(s) 20, 489, 1297, 2035
BpiI GAAGAC 1 cut(s) 459
BpmI CTGGAG 3 cut(s) 579, 840, 918
Bpu14I TTCGAA 1 cut(s) 1109
BpuEI CTTGAG 3 cut(s) 456, 1650, 1733
Bsa29I ATCGAT 1 cut(s) 73
BsaAI YACGTR 1 cut(s) 2381
BsaBI GATNNNNATC 2 cut(s) 741, 3018
BsaHI GRCGYC 2 cut(s) 486, 728
BsaI GGTCTC 1 cut(s) 471
BsaJI CCNNGG 8 cut(s) 731, 732, 867, 1002, 1066, 1987, 2590, 2892
BsaWI WCCGGW 1 cut(s) 435
Bsc4I CCNNNNNNNGG 8 cut(s) 161, 680, 867, 868, 1021, 1375, 1769, 1897
Bse8I GATNNNNATC 2 cut(s) 741, 3018
BseBI CCWGG 4 cut(s) 733, 819, 868, 2163
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 8 cut(s) 731, 732, 867, 1002, 1066, 1987, 2590, 2892
BseGI GGATG 7 cut(s) 444, 1215, 1315, 1351, 1981, 2342, 2952
BseJI GATNNNNATC 2 cut(s) 741, 3018
BseLI CCNNNNNNNGG 8 cut(s) 161, 680, 867, 868, 1021, 1375, 1769, 1897
BseMII CTCAG 8 cut(s) 72, 129, 208, 582, 2274, 2308, 2405, 2441
BseRI GAGGAG 3 cut(s) 459, 1490, 2501
BseSI GKGCMC 1 cut(s) 2539
BseXI GCAGC 9 cut(s) 534, 682, 931, 2018, 2117, 2143, 2408, 2411, 2551
BseYI CCCAGC 1 cut(s) 1413
Bsh1236I CGCG 3 cut(s) 458, 2073, 2760
BshFI GGCC 2 cut(s) 1986, 2030
BshVI ATCGAT 1 cut(s) 73
BsiHKAI GWGCWC 4 cut(s) 195, 1742, 2281, 2497
BsiHKCI CYCGRG 1 cut(s) 1470
BsiSI CCGG 1 cut(s) 436
BslFI GGGAC 2 cut(s) 497, 1352
BslI CCNNNNNNNGG 8 cut(s) 161, 680, 867, 868, 1021, 1375, 1769, 1897
BsmAI GTCTC 4 cut(s) 471, 1472, 1668, 2731
BsmBI CGTCTC 1 cut(s) 2731
BsmFI GGGAC 2 cut(s) 497, 1352
BsnI GGCC 2 cut(s) 1986, 2030
Bso31I GGTCTC 1 cut(s) 471
BsoBI CYCGRG 1 cut(s) 1470
Bsp119I TTCGAA 1 cut(s) 1109
Bsp1286I GDGCHC 6 cut(s) 195, 1742, 2281, 2428, 2497, 2539
Bsp143I GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
Bsp19I CCATGG 2 cut(s) 1002, 2892
Bsp68I TCGCGA 1 cut(s) 2073
BspACI CCGC 6 cut(s) 5, 278, 458, 2091, 2562, 2760
BspANI GGCC 2 cut(s) 1986, 2030
BspCNI CTCAG 8 cut(s) 71, 130, 207, 581, 2273, 2307, 2404, 2440
BspDI ATCGAT 1 cut(s) 73
BspFNI CGCG 3 cut(s) 458, 2073, 2760
BspHI TCATGA 3 cut(s) 31, 262, 1854
BspLI GGNNCC 3 cut(s) 1064, 1315, 2334
BspPI GGATC 2 cut(s) 698, 1851
BspT104I TTCGAA 1 cut(s) 1109
BspTI CTTAAG 1 cut(s) 2272
BspTNI GGTCTC 1 cut(s) 471
BssECI CCNNGG 8 cut(s) 731, 732, 867, 1002, 1066, 1987, 2590, 2892
BssMI GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
BssNI GRCGYC 2 cut(s) 486, 728
BssT1I CCWWGG 4 cut(s) 1002, 1066, 1987, 2892
Bst2UI CCWGG 4 cut(s) 733, 819, 868, 2163
Bst6I CTCTTC 2 cut(s) 867, 1579
BstACI GRCGYC 2 cut(s) 486, 728
BstAFI CTTAAG 1 cut(s) 2272
BstAPI GCANNNNNTGC 1 cut(s) 940
BstBAI YACGTR 1 cut(s) 2381
BstBI TTCGAA 1 cut(s) 1109
BstC8I GCNNGC 5 cut(s) 675, 1624, 2493, 2508, 2559
BstDEI CTNAG 9 cut(s) 58, 138, 194, 568, 2032, 2260, 2294, 2391, 2427
BstDSI CCRYGG 2 cut(s) 1002, 2892
BstENI CCTNNNNNAGG 3 cut(s) 678, 1373, 1895
BstF5I GGATG 7 cut(s) 444, 1215, 1315, 1351, 1981, 2342, 2952
BstFNI CGCG 3 cut(s) 458, 2073, 2760
BstHHI GCGC 2 cut(s) 313, 2760
BstKTI GATC 8 cut(s) 184, 541, 706, 1051, 1846, 2218, 2920, 3022
BstMAI GTCTC 4 cut(s) 471, 1472, 1668, 2731
BstMBI GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
BstMWI GCNNNNNNNGC 7 cut(s) 617, 940, 1212, 2153, 2276, 2346, 2393
BstNI CCWGG 4 cut(s) 733, 819, 868, 2163
BstNSI RCATGY 3 cut(s) 1507, 1608, 1626
BstSCI CCNGG 4 cut(s) 731, 817, 866, 2161
BstSFI CTRYAG 1 cut(s) 2683
BstSLI GKGCMC 1 cut(s) 2539
BstSNI TACGTA 1 cut(s) 2381
BstUI CGCG 3 cut(s) 458, 2073, 2760
BstV1I GCAGC 9 cut(s) 534, 682, 931, 2018, 2117, 2143, 2408, 2411, 2551
BstV2I GAAGAC 1 cut(s) 459
BstXI CCANNNNNNTGG 1 cut(s) 1618
Bsu15I ATCGAT 1 cut(s) 73
BsuRI GGCC 2 cut(s) 1986, 2030
BsuTUI ATCGAT 1 cut(s) 73
BtgI CCRYGG 2 cut(s) 1002, 2892
BtgZI GCGATG 1 cut(s) 2088
BtsCI GGATG 7 cut(s) 444, 1215, 1315, 1351, 1981, 2342, 2952
BtsI GCAGTG 1 cut(s) 1633
BtsIMutI CAGTG 6 cut(s) 502, 934, 1449, 1633, 1786, 2709
BtuMI TCGCGA 1 cut(s) 2073
Cac8I GCNNGC 5 cut(s) 675, 1624, 2493, 2508, 2559
CaiI CAGNNNCTG 1 cut(s) 351
CciI TCATGA 3 cut(s) 31, 262, 1854
CfoI GCGC 2 cut(s) 313, 2760
Cfr13I GGNCC 6 cut(s) 946, 1314, 1961, 2028, 2088, 3090
ClaI ATCGAT 1 cut(s) 73
CseI GACGC 4 cut(s) 351, 464, 494, 736
Csp6I GTAC 2 cut(s) 1053, 2378
CviQI GTAC 2 cut(s) 1053, 2378
DdeI CTNAG 9 cut(s) 58, 138, 194, 568, 2032, 2260, 2294, 2391, 2427
DpnI GATC 8 cut(s) 183, 540, 705, 1050, 1845, 2217, 2919, 3021
DpnII GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
DraIII CACNNNGTG 1 cut(s) 2543
EaeI YGGCCR 1 cut(s) 1984
Eam1104I CTCTTC 2 cut(s) 867, 1579
EarI CTCTTC 2 cut(s) 867, 1579
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 2279
Eco105I TACGTA 1 cut(s) 2381
Eco130I CCWWGG 4 cut(s) 1002, 1066, 1987, 2892
Eco24I GRGCYC 2 cut(s) 2281, 2428
Eco31I GGTCTC 1 cut(s) 471
Eco47I GGWCC 5 cut(s) 946, 1314, 1961, 2088, 3090
Eco53kI GAGCTC 1 cut(s) 2279
Eco57I CTGAAG 5 cut(s) 187, 344, 471, 650, 2928
Eco88I CYCGRG 1 cut(s) 1470
EcoICRI GAGCTC 1 cut(s) 2279
EcoNI CCTNNNNNAGG 3 cut(s) 678, 1373, 1895
EcoRI GAATTC 1 cut(s) 1640
EcoRII CCWGG 4 cut(s) 731, 817, 866, 2161
EcoT14I CCWWGG 4 cut(s) 1002, 1066, 1987, 2892
EcoT22I ATGCAT 1 cut(s) 1575
EcoT38I GRGCYC 2 cut(s) 2281, 2428
ErhI CCWWGG 4 cut(s) 1002, 1066, 1987, 2892
Esp3I CGTCTC 1 cut(s) 2731
FalI AAGNNNNNCTT 4 cut(s) 1402, 1434, 2194, 2226
FaqI GGGAC 2 cut(s) 497, 1352
FauI CCCGC 2 cut(s) 271, 2753
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 3 cut(s) 792, 991, 1697
FokI GGATG 7 cut(s) 451, 1222, 1322, 1358, 1988, 2329, 2939
FriOI GRGCYC 2 cut(s) 2281, 2428
FspAI RTGCGCAY 1 cut(s) 312
FspBI CTAG 6 cut(s) 987, 1103, 1332, 2219, 2337, 2913
FspI TGCGCA 1 cut(s) 312
GlaI GCGC 2 cut(s) 312, 2759
GsaI CCCAGC 1 cut(s) 1417
GsuI CTGGAG 3 cut(s) 579, 840, 918
HaeIII GGCC 2 cut(s) 1986, 2030
HapII CCGG 1 cut(s) 436
HgaI GACGC 4 cut(s) 351, 464, 494, 736
HhaI GCGC 2 cut(s) 313, 2760
Hin1I GRCGYC 2 cut(s) 486, 728
Hin6I GCGC 2 cut(s) 311, 2758
HinP1I GCGC 2 cut(s) 311, 2758
HincII GTYRAC 2 cut(s) 2370, 2947
HindII GTYRAC 2 cut(s) 2370, 2947
HindIII AAGCTT 2 cut(s) 1915, 2471
HpaII CCGG 1 cut(s) 436
HphI GGTGA 1 cut(s) 2734
HpyAV CCTTC 9 cut(s) 247, 268, 1169, 1374, 1943, 1973, 2220, 2526, 2713
HpyCH4IV ACGT 2 cut(s) 2380, 2731
HpyF10VI GCNNNNNNNGC 7 cut(s) 617, 940, 1212, 2153, 2276, 2346, 2393
HpyF3I CTNAG 9 cut(s) 58, 138, 194, 568, 2032, 2260, 2294, 2391, 2427
HpySE526I ACGT 2 cut(s) 2380, 2731
Hsp92I GRCGYC 2 cut(s) 486, 728
HspAI GCGC 2 cut(s) 311, 2758
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
LmnI GCTCC 8 cut(s) 1081, 1126, 1570, 2164, 2338, 2488, 2515, 2747
Lsp1109I GCAGC 9 cut(s) 534, 682, 931, 2018, 2117, 2143, 2408, 2411, 2551
LweI GCATC 7 cut(s) 429, 598, 1237, 1401, 1839, 2493, 2781
MaeI CTAG 6 cut(s) 987, 1103, 1332, 2219, 2337, 2913
MaeII ACGT 2 cut(s) 2380, 2731
MalI GATC 8 cut(s) 183, 540, 705, 1050, 1845, 2217, 2919, 3021
MboI GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
MfeI CAATTG 2 cut(s) 220, 1793
MhlI GDGCHC 6 cut(s) 195, 1742, 2281, 2428, 2497, 2539
MlsI TGGCCA 1 cut(s) 1986
MluNI TGGCCA 1 cut(s) 1986
MlyI GAGTC 8 cut(s) 560, 1007, 1265, 1369, 1462, 1670, 1703, 2357
MmeI TCCRAC 7 cut(s) 25, 1635, 1821, 2066, 2463, 2714, 3024
Mox20I TGGCCA 1 cut(s) 1986
Mph1103I ATGCAT 1 cut(s) 1575
MroXI GAANNNNTTC 3 cut(s) 333, 876, 2585
MscI TGGCCA 1 cut(s) 1986
MslI CAYNNNNRTG 3 cut(s) 2544, 3062, 3085
Msp20I TGGCCA 1 cut(s) 1986
MspA1I CMGCKG 2 cut(s) 2375, 2564
MspCI CTTAAG 1 cut(s) 2272
MspI CCGG 1 cut(s) 436
MspR9I CCNGG 4 cut(s) 733, 819, 868, 2163
MunI CAATTG 2 cut(s) 220, 1793
MvaI CCWGG 4 cut(s) 733, 819, 868, 2163
MvnI CGCG 3 cut(s) 458, 2073, 2760
MwoI GCNNNNNNNGC 7 cut(s) 617, 940, 1212, 2153, 2276, 2346, 2393
NcoI CCATGG 2 cut(s) 1002, 2892
NdeII GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
NlaIV GGNNCC 3 cut(s) 1064, 1315, 2334
NmeAIII GCCGAG 1 cut(s) 1684
NmuCI GTSAC 5 cut(s) 215, 1322, 1447, 1788, 2234
NruI TCGCGA 1 cut(s) 2073
NsbI TGCGCA 1 cut(s) 312
NsiI ATGCAT 1 cut(s) 1575
NspI RCATGY 3 cut(s) 1507, 1608, 1626
NspV TTCGAA 1 cut(s) 1109
PaeI GCATGC 1 cut(s) 1626
PagI TCATGA 3 cut(s) 31, 262, 1854
PasI CCCWGGG 1 cut(s) 732
PciI ACATGT 1 cut(s) 1604
PcsI WCGNNNNNNNCGW 1 cut(s) 79
PdmI GAANNNNTTC 3 cut(s) 333, 876, 2585
PflMI CCANNNNNTGG 1 cut(s) 161
PfoI TCCNGGA 2 cut(s) 817, 2161
PleI GAGTC 8 cut(s) 560, 1006, 1264, 1368, 1462, 1669, 1702, 2357
PpsI GAGTC 8 cut(s) 560, 1006, 1264, 1368, 1462, 1669, 1702, 2357
Ppu21I YACGTR 1 cut(s) 2381
PscI ACATGT 1 cut(s) 1604
Psp124BI GAGCTC 1 cut(s) 2281
Psp6I CCWGG 4 cut(s) 731, 817, 866, 2161
PspFI CCCAGC 1 cut(s) 1413
PspGI CCWGG 4 cut(s) 731, 817, 866, 2161
PspN4I GGNNCC 3 cut(s) 1064, 1315, 2334
PspPI GGNCC 6 cut(s) 946, 1314, 1961, 2028, 2088, 3090
PstNI CAGNNNCTG 1 cut(s) 351
PvuII CAGCTG 1 cut(s) 2375
RruI TCGCGA 1 cut(s) 2073
RsaI GTAC 2 cut(s) 1054, 2379
RsaNI GTAC 2 cut(s) 1053, 2378
RseI CAYNNNNRTG 3 cut(s) 2544, 3062, 3085
SacI GAGCTC 1 cut(s) 2281
Sau3AI GATC 8 cut(s) 181, 538, 703, 1048, 1843, 2215, 2917, 3019
Sau96I GGNCC 6 cut(s) 946, 1314, 1961, 2028, 2088, 3090
SchI GAGTC 8 cut(s) 560, 1007, 1265, 1369, 1462, 1670, 1703, 2357
ScrFI CCNGG 4 cut(s) 733, 819, 868, 2163
SduI GDGCHC 6 cut(s) 195, 1742, 2281, 2428, 2497, 2539
SfaNI GCATC 7 cut(s) 429, 598, 1237, 1401, 1839, 2493, 2781
SfcI CTRYAG 1 cut(s) 2683
SfuI TTCGAA 1 cut(s) 1109
SinI GGWCC 5 cut(s) 946, 1314, 1961, 2088, 3090
SmiMI CAYNNNNRTG 3 cut(s) 2544, 3062, 3085
SmlI CTYRAG 4 cut(s) 471, 1665, 1712, 2272
SmoI CTYRAG 4 cut(s) 471, 1665, 1712, 2272
SnaBI TACGTA 1 cut(s) 2381
SphI GCATGC 1 cut(s) 1626
SsiI CCGC 6 cut(s) 5, 278, 458, 2091, 2562, 2760
SspMI CTAG 6 cut(s) 987, 1103, 1332, 2219, 2337, 2913
SstI GAGCTC 1 cut(s) 2281
StyD4I CCNGG 4 cut(s) 731, 817, 866, 2161
StyI CCWWGG 4 cut(s) 1002, 1066, 1987, 2892
TaiI ACGT 2 cut(s) 2383, 2734
TaqI TCGA 9 cut(s) 73, 184, 399, 957, 1109, 1644, 1742, 1824, 2501
TaqII GACCGA 1 cut(s) 453
TauI GCSGC 2 cut(s) 461, 2564
TscAI CASTG 6 cut(s) 502, 934, 1456, 1633, 1786, 2709
TseFI GTSAC 5 cut(s) 215, 1322, 1447, 1788, 2234
TseI GCWGC 9 cut(s) 522, 670, 919, 2006, 2105, 2156, 2396, 2399, 2564
Tsp45I GTSAC 5 cut(s) 215, 1322, 1447, 1788, 2234
TspGWI ACGGA 3 cut(s) 2542, 2755, 2814
TspRI CASTG 6 cut(s) 502, 934, 1456, 1633, 1786, 2709
Van91I CCANNNNNTGG 1 cut(s) 161
Vha464I CTTAAG 1 cut(s) 2272
VpaK11BI GGWCC 5 cut(s) 946, 1314, 1961, 2088, 3090
XagI CCTNNNNNAGG 3 cut(s) 678, 1373, 1895
XapI RAATTY 8 cut(s) 102, 129, 1030, 1457, 1640, 1901, 2456, 2986
XceI RCATGY 3 cut(s) 1507, 1608, 1626
XmiI GTMKAC 3 cut(s) 792, 991, 1697
XmnI GAANNNNTTC 3 cut(s) 333, 876, 2585
XspI CTAG 6 cut(s) 987, 1103, 1332, 2219, 2337, 2913
Zsp2I ATGCAT 1 cut(s) 1575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.