FvH4_1g20721

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001949
Physical Location & Seq
Reverse (-)
12872727 .. 12878013
5287 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20721.t2

Sequence Viewer

Length: 2640 bp
ATGGCGGCTAGGAAGCGACTGCGTCCTGATGATCAATTAGAGATTCCTCAGATACTTTTACAAGCACACCATCGATGGTTACACCCTGCTGAAATTTGCCAAATTCTTCGGAATCATACAAAATTTAGGCTTTCTGAGGAGCATGCAGATACTCCAGAGGGTGGAACATTGTCTCTCTATGATCGGAAGGTGCTCAGGTCTTTCAGAAAAGATGGTTATAAATGGAAGAGGACTAAAGACGGAAAGGCAGTGAAGGAAGCCCATGAGAGGCTCAAGGTTGGAGGAGTAGAGGTGTTACATTGCTACTATGCCCATGGTGAAGAAAATGAAAATTTCCAAAGACGCTGTTATTGGATGATTGAAAAGCGTCTCTCCCACATAGTTCTGGTCCACTACCGGGATGTAAAGACTTCTGAAGGTAACTTATCATGTTCATCCGAGTTGTCTATCAATCGGAATGTGCATTTGGATATCTCATATCATGCCAGTACTTCGTTCCCTCGAGGAGTCAATGCAGATGAGTTACTCAATTCCCTGGGGCCTCCTTGTCATATGGATTTAATTAAACAAAATGAGTCTTCTCTGCATAGGCACTCTGATAATAAAAATGACCCCTGGAGATTGAAGAATCTTGATAGTTCCAATCAATGTATGAATACAAATCTAGGAGACCAAACTGAGCCACATATGTCAAGTTCTGAGGACCACTCGAATACTATAGCAAGTGAGAATGGGATTGATGGCTTCAGTGCTCTTAAGCAAGTCGAAATAGCTACTCTGGGATCTTCTCTATTCCAGGATCTTCTCTTTAACATTATGGACTTTTCACCCAACTGGGCATATGAACGCTCTGAAACAAAGGTTGTTGTTGTGGGAAGATTCTTGAAGAGTCAAGAATTAGACAGTTTTAGTTGGTCATGTATGTTTGGGGAAGTAGAAGTTCCCGCTGAGGTTGTAGCAGATGGTGTTCTTCGTTGTCATACGCCCATACATCGGGCTGCTAGGATTCCCTTCTATGTAACATGTTCCAATAGGTTAGCATGTAGTGAAGTGAAAATATTTGAGTACCGAGTCAACCATATACAAGACGCAGATTTTAAGGATAACTGCTGCAAAGAAAATACACTTGTTATGCGTTTTGGGAAGTTACTGTCCCCAAGCGTTATGTATCCAAGTTTCAATCCCAGGGTTAAACATAGACAGTATGATCCCATTTGTGTATCAGACAATTCTGATTTGACTAGGAAAATTAGTTCATTACTCAAAAATGGTGATGAGGAAAGAGCTGAGATGCTAAAGCTTTTTCCAGATGCGTTTTCCTTGGATACAGTAAAGGAGCAGCTGCTTCAACAGTTTCTTAAAGATAAGTTTCATGAATGGCTCTTGAAGAAGCTGGCTGTTGGTGGAAATGGCTTAAGGGTACTGGATGAAGGCGGCCAAGGTGTGTTACATTTTGGAGCTGCTCTTGGCTATGACTGGGTCTTATCACCCATGAAAATTGCTGGAGTCAGTATCAATTTTCGTGATGTGAATGGATGGACGGCACTTCATTGGGCAGCCTTTTGTGGCAGAGACCGCGCAGTTGCTTTGCTCATCAGTCTTGGTGGAGCTCCTGGAGCATTAACTGATCCTTGTACCAAAAATCCTACTGGCAAAACACCAGCTGACCTAGCTTCTGAAAATGGATACAAAGGAATTGCTGGTTATCTTGCAGCATCTTCTTTGAGTGCCCACATTTCATCTCTTAAATTGGATATCAAAGATGCACATTCTGCAGAGAGTTCAAGAGAAAAATCAGTACAACCAGATGGAATGTCAGCACATTGCAGCAATGGGGACTTAGCGGATGAGCTCTCCATGAGGGTTTGTTCAACTGCTGTTTGTGATCATATCCAAGCTTCTGCTCGTATTCATCAAGTATTCAGTGTACAACCTTTCCAAAGGAAGCAGTTGAAAGAGTATGGTGATAAATCAAAAACTTCAGATGAAGGGGCACTATCGCTCAGTGCAGTCAAGTCACATAAGAAATGTGACCAGCATGTGAGTTGTGCTGCAATACAAACGCCTGATAAATTTCGTAATCGGAATATAAAAGACTTTCTGATAATCCGGAGCAGAATTCTCAAAATTCAGGCAAATGTTAGAGGTCACCAGGAGAGGAAAAGTTATACAAAGATACTATGGGCAGTAGGAATTTTAGAGAAGATCATTTTACGTTGGAGACGTAAAAGAAGTGGCTTGCGCGGGTTCAAGCGAGAACCACCTAGTGATGAGGGCCGAAGCATACAAGTTTCATCCTCAAGGGAGAATGATTATGACTTTTTGAAAGAGGGAAGGAAGCAAACAGAGGAAAGGTTGCAAAATGCACTTGCTAGGGTGAAGTCCATGGTTCAGATTCCTGAAGCAAGAGATCAATACAATCAACTGTCGAATATTTTCACCGAGATTCAGGAAACAAAGGTGGTGGACAGCTCAGAAGGGGAATCCACTGATATGGATTATAACGGGATGTTGCAGGTGGTGAACAACTCGGTTGGGATATCGGCTGATATGGACTATAACATCATAGACACCAATGCCCTCCTGGATGACAACATTTGTATGTATGACCACATCTGTATAGATGATCTCTGGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

880

Amino Acids

99.41

Weight (kDa)

6.92

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 4 - 84 2.4e-34 CG-1 domain
Ank_2 PF12796 434 - 522 7.4e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 219, 2502
AarI CACCTGC 1 cut(s) 2506
AbsI CCTCGAGG 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 2506
AccB7I CCANNNNNTGG 1 cut(s) 161
AccII CGCG 2 cut(s) 1578, 2244
AccIII TCCGGA 1 cut(s) 2109
AciI CCGC 6 cut(s) 5, 945, 1434, 1576, 1844, 2244
AclWI GGATC 4 cut(s) 790, 807, 1202, 1622
AcoI YGGCCR 1 cut(s) 1435
AcsI RAATTY 8 cut(s) 93, 102, 122, 331, 2072, 2118, 2127, 2193
AcuI CTGAAG 4 cut(s) 435, 730, 1965, 2421
AdeI CACNNNGTG 1 cut(s) 2267
AfaI GTAC 6 cut(s) 490, 1067, 1422, 1636, 1800, 1929
AfiI CCNNNNNNNGG 4 cut(s) 161, 267, 397, 993
AflII CTTAAG 2 cut(s) 755, 1414
AflIII ACRYGT 1 cut(s) 1022
AjnI CCWGG 7 cut(s) 534, 614, 795, 1184, 1612, 2151, 2583
AjuI GAANNNNNNNTTGG 2 cut(s) 449, 481
AloI GAACNNNNNNTCC 2 cut(s) 1237, 1269
Alw21I GWGCWC 4 cut(s) 195, 754, 1612, 1854
Alw26I GTCTC 5 cut(s) 177, 374, 663, 1566, 2215
AlwI GGATC 4 cut(s) 790, 807, 1202, 1622
Ama87I CYCGRG 1 cut(s) 501
Aor13HI TCCGGA 1 cut(s) 2109
AoxI GGCC 3 cut(s) 539, 1435, 2275
ApeKI GCWGC 9 cut(s) 998, 1110, 1339, 1342, 1460, 1556, 1712, 1827, 2051
ApoI RAATTY 8 cut(s) 93, 102, 122, 331, 2072, 2118, 2127, 2193
Asp700I GAANNNNTTC 1 cut(s) 2435
AspLEI GCGC 2 cut(s) 1580, 2244
AspS9I GGNCC 4 cut(s) 388, 539, 703, 2275
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 9 cut(s) 329, 819, 1283, 1479, 1976, 2141, 2389, 2431, 2533
AvaI CYCGRG 1 cut(s) 501
AvaII GGWCC 2 cut(s) 388, 703
BaeGI GKGCMC 2 cut(s) 1732, 1996
BanII GRGCYC 2 cut(s) 1612, 1854
BbsI GAAGAC 1 cut(s) 570
Bbv12I GWGCWC 4 cut(s) 195, 754, 1612, 1854
BbvCI CCTCAGC 1 cut(s) 948
BbvI GCAGC 9 cut(s) 985, 1097, 1329, 1351, 1447, 1568, 1724, 1839, 2038
BccI CCATC 7 cut(s) 69, 78, 206, 734, 956, 1530, 1802
BceAI ACGGC 1 cut(s) 1557
BciT130I CCWGG 7 cut(s) 536, 616, 797, 1186, 1614, 2153, 2585
BciVI GTATCC 3 cut(s) 1179, 1318, 1679
BclI TGATCA 2 cut(s) 31, 1885
BcnI CCSGG 1 cut(s) 398
BcoDI GTCTC 5 cut(s) 177, 374, 663, 1566, 2215
BfaI CTAG 7 cut(s) 9, 665, 1002, 1242, 1670, 2265, 2373
BfmI CTRYAG 2 cut(s) 717, 1773
BfrI CTTAAG 2 cut(s) 755, 1414
BfuAI ACCTGC 1 cut(s) 2506
BfuI GTATCC 3 cut(s) 1179, 1318, 1679
BmcAI AGTACT 1 cut(s) 490
Bme1390I CCNGG 8 cut(s) 398, 536, 616, 797, 1186, 1614, 2153, 2585
Bme18I GGWCC 2 cut(s) 388, 703
BmeT110I CYCGRG 1 cut(s) 501
BmgT120I GGNCC 4 cut(s) 388, 539, 703, 2275
BmiI GGNNCC 1 cut(s) 540
BmrFI CCNGG 8 cut(s) 398, 536, 616, 797, 1186, 1614, 2153, 2585
BmrI ACTGGG 2 cut(s) 844, 1486
BmsI GCATC 4 cut(s) 1281, 1300, 1724, 1753
BmuI ACTGGG 2 cut(s) 844, 1486
BpiI GAAGAC 1 cut(s) 570
BplI GAGNNNNNCTC 2 cut(s) 692, 724
BpmI CTGGAG 4 cut(s) 138, 637, 1524, 1635
Bpu10I CCTNAGC 2 cut(s) 194, 948
BpuEI CTTGAG 2 cut(s) 257, 2284
BpuMI CCSGG 1 cut(s) 398
Bsa29I ATCGAT 1 cut(s) 73
BsaI GGTCTC 2 cut(s) 663, 1566
BsaJI CCNNGG 9 cut(s) 313, 534, 535, 614, 1184, 1185, 1320, 1438, 2385
BsaWI WCCGGW 1 cut(s) 2109
BsaXI ACNNNNNCTCC 2 cut(s) 276, 306
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 267, 397, 993
Bse1I ACTGG 5 cut(s) 486, 839, 1428, 1481, 1654
Bse3DI GCAATG 3 cut(s) 298, 1822, 1837
BseAI TCCGGA 1 cut(s) 2109
BseBI CCWGG 7 cut(s) 536, 616, 797, 1186, 1614, 2153, 2585
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 9 cut(s) 313, 534, 535, 614, 1184, 1185, 1320, 1438, 2385
BseGI GGATG 9 cut(s) 360, 406, 434, 1432, 1541, 1852, 2294, 2514, 2593
BseLI CCNNNNNNNGG 4 cut(s) 161, 267, 397, 993
BseMI GCAATG 3 cut(s) 298, 1822, 1837
BseMII CTCAG 9 cut(s) 62, 126, 208, 669, 690, 939, 1278, 2017, 2487
BseNI ACTGG 5 cut(s) 486, 839, 1428, 1481, 1654
BseRI GAGGAG 3 cut(s) 152, 297, 519
BseSI GKGCMC 2 cut(s) 1732, 1996
BseXI GCAGC 9 cut(s) 985, 1097, 1329, 1351, 1447, 1568, 1724, 1839, 2038
BsgI GTGCAG 1 cut(s) 2028
Bsh1236I CGCG 2 cut(s) 1578, 2244
BshFI GGCC 3 cut(s) 541, 1437, 2277
BshVI ATCGAT 1 cut(s) 73
BsiHKAI GWGCWC 4 cut(s) 195, 754, 1612, 1854
BsiHKCI CYCGRG 1 cut(s) 501
BsiSI CCGG 2 cut(s) 397, 2110
BslFI GGGAC 2 cut(s) 1138, 1850
BslI CCNNNNNNNGG 4 cut(s) 161, 267, 397, 993
BsmAI GTCTC 5 cut(s) 177, 374, 663, 1566, 2215
BsmBI CGTCTC 2 cut(s) 374, 2215
BsmFI GGGAC 2 cut(s) 1138, 1850
BsnI GGCC 3 cut(s) 541, 1437, 2277
Bso31I GGTCTC 2 cut(s) 663, 1566
BsoBI CYCGRG 1 cut(s) 501
Bsp1286I GDGCHC 6 cut(s) 195, 754, 1612, 1732, 1854, 1996
Bsp13I TCCGGA 1 cut(s) 2109
Bsp1407I TGTACA 1 cut(s) 1927
Bsp19I CCATGG 2 cut(s) 313, 2385
BspACI CCGC 6 cut(s) 5, 945, 1434, 1576, 1844, 2244
BspANI GGCC 3 cut(s) 541, 1437, 2277
BspCNI CTCAG 9 cut(s) 61, 127, 207, 670, 691, 940, 1279, 2016, 2486
BspDI ATCGAT 1 cut(s) 73
BspEI TCCGGA 1 cut(s) 2109
BspFNI CGCG 2 cut(s) 1578, 2244
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 1 cut(s) 540
BspMAI CTGCAG 1 cut(s) 1777
BspMI ACCTGC 1 cut(s) 2506
BspPI GGATC 4 cut(s) 790, 807, 1202, 1622
BspTI CTTAAG 2 cut(s) 755, 1414
BspTNI GGTCTC 2 cut(s) 663, 1566
BsrDI GCAATG 3 cut(s) 298, 1822, 1837
BsrGI TGTACA 1 cut(s) 1927
BsrI ACTGG 5 cut(s) 486, 839, 1428, 1481, 1654
BssECI CCNNGG 9 cut(s) 313, 534, 535, 614, 1184, 1185, 1320, 1438, 2385
BssT1I CCWWGG 4 cut(s) 313, 1320, 1438, 2385
Bst2UI CCWGG 7 cut(s) 536, 616, 797, 1186, 1614, 2153, 2585
Bst4CI ACNGT 6 cut(s) 905, 1152, 1203, 1330, 1353, 2427
Bst6I CTCTTC 2 cut(s) 221, 881
BstAFI CTTAAG 2 cut(s) 755, 1414
BstAPI GCANNNNNTGC 1 cut(s) 1772
BstAUI TGTACA 1 cut(s) 1927
BstC8I GCNNGC 3 cut(s) 144, 1395, 2240
BstDSI CCRYGG 2 cut(s) 313, 2385
BstEII GGTNACC 1 cut(s) 2147
BstF5I GGATG 9 cut(s) 360, 406, 434, 1432, 1541, 1852, 2294, 2514, 2593
BstFNI CGCG 2 cut(s) 1578, 2244
BstHHI GCGC 2 cut(s) 1580, 2244
BstMAI GTCTC 5 cut(s) 177, 374, 663, 1566, 2215
BstMWI GCNNNNNNNGC 4 cut(s) 1575, 1616, 1670, 1772
BstNI CCWGG 7 cut(s) 536, 616, 797, 1186, 1614, 2153, 2585
BstNSI RCATGY 4 cut(s) 146, 1026, 1044, 2042
BstPI GGTNACC 1 cut(s) 2147
BstSCI CCNGG 8 cut(s) 396, 534, 614, 795, 1184, 1612, 2151, 2583
BstSFI CTRYAG 2 cut(s) 717, 1773
BstSLI GKGCMC 2 cut(s) 1732, 1996
BstUI CGCG 2 cut(s) 1578, 2244
BstV1I GCAGC 9 cut(s) 985, 1097, 1329, 1351, 1447, 1568, 1724, 1839, 2038
BstV2I GAAGAC 1 cut(s) 570
BstX2I RGATCY 2 cut(s) 782, 799
BstXI CCANNNNNNTGG 1 cut(s) 2494
BstYI RGATCY 2 cut(s) 782, 799
Bsu15I ATCGAT 1 cut(s) 73
BsuI GTATCC 3 cut(s) 1179, 1318, 1679
BsuRI GGCC 3 cut(s) 541, 1437, 2277
BsuTUI ATCGAT 1 cut(s) 73
BtgI CCRYGG 2 cut(s) 313, 2385
BtsCI GGATG 9 cut(s) 360, 406, 434, 1432, 1541, 1852, 2294, 2514, 2593
BtsI GCAGTG 1 cut(s) 255
BtsIMutI CAGTG 5 cut(s) 255, 754, 1930, 2011, 2487
BveI ACCTGC 1 cut(s) 2506
Cac8I GCNNGC 3 cut(s) 144, 1395, 2240
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 2 cut(s) 1580, 2244
Cfr13I GGNCC 4 cut(s) 388, 539, 703, 2275
ClaI ATCGAT 1 cut(s) 73
CseI GACGC 4 cut(s) 11, 351, 356, 1097
Csp6I GTAC 6 cut(s) 489, 1066, 1421, 1635, 1799, 1928
CspCI CAANNNNNGTGG 2 cut(s) 2445, 2480
CviQI GTAC 6 cut(s) 489, 1066, 1421, 1635, 1799, 1928
DraIII CACNNNGTG 1 cut(s) 2267
EaeI YGGCCR 1 cut(s) 1435
Eam1104I CTCTTC 2 cut(s) 221, 881
EarI CTCTTC 2 cut(s) 221, 881
Ecl136II GAGCTC 2 cut(s) 1610, 1852
Eco130I CCWWGG 4 cut(s) 313, 1320, 1438, 2385
Eco24I GRGCYC 2 cut(s) 1612, 1854
Eco31I GGTCTC 2 cut(s) 663, 1566
Eco32I GATATC 3 cut(s) 472, 1756, 2541
Eco47I GGWCC 2 cut(s) 388, 703
Eco53kI GAGCTC 2 cut(s) 1610, 1852
Eco57I CTGAAG 4 cut(s) 435, 730, 1965, 2421
Eco88I CYCGRG 1 cut(s) 501
Eco91I GGTNACC 1 cut(s) 2147
EcoICRI GAGCTC 2 cut(s) 1610, 1852
EcoO109I RGGNCCY 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 2147
EcoRI GAATTC 1 cut(s) 2118
EcoRII CCWGG 7 cut(s) 534, 614, 795, 1184, 1612, 2151, 2583
EcoRV GATATC 3 cut(s) 472, 1756, 2541
EcoT14I CCWWGG 4 cut(s) 313, 1320, 1438, 2385
EcoT38I GRGCYC 2 cut(s) 1612, 1854
ErhI CCWWGG 4 cut(s) 313, 1320, 1438, 2385
Esp3I CGTCTC 2 cut(s) 374, 2215
FaqI GGGAC 2 cut(s) 1138, 1850
FauI CCCGC 2 cut(s) 952, 2237
FauNDI CATATG 3 cut(s) 552, 687, 841
FbaI TGATCA 2 cut(s) 31, 1885
FokI GGATG 9 cut(s) 367, 413, 421, 1439, 1548, 1859, 2281, 2521, 2600
FriOI GRGCYC 2 cut(s) 1612, 1854
FspBI CTAG 7 cut(s) 9, 665, 1002, 1242, 1670, 2265, 2373
GlaI GCGC 2 cut(s) 1579, 2243
GsuI CTGGAG 4 cut(s) 138, 637, 1524, 1635
HaeIII GGCC 3 cut(s) 541, 1437, 2277
HapII CCGG 2 cut(s) 397, 2110
HgaI GACGC 4 cut(s) 11, 351, 356, 1097
HhaI GCGC 2 cut(s) 1580, 2244
Hin6I GCGC 2 cut(s) 1578, 2242
HinP1I GCGC 2 cut(s) 1578, 2242
HincII GTYRAC 1 cut(s) 1075
HindII GTYRAC 1 cut(s) 1075
HindIII AAGCTT 2 cut(s) 1298, 1896
HpaII CCGG 2 cut(s) 397, 2110
HphI GGTGA 9 cut(s) 329, 819, 1283, 1479, 1976, 2141, 2389, 2431, 2533
Hpy166II GTNNAC 5 cut(s) 391, 1075, 1928, 2467, 2524
Hpy8I GTNNAC 5 cut(s) 391, 1075, 1928, 2467, 2524
HpyAV CCTTC 8 cut(s) 181, 247, 410, 1021, 1424, 1982, 2328, 2471
HpyCH4III ACNGT 6 cut(s) 905, 1152, 1203, 1330, 1353, 2427
HpyCH4IV ACGT 2 cut(s) 2215, 2224
HpyF10VI GCNNNNNNNGC 4 cut(s) 1575, 1616, 1670, 1772
HpySE526I ACGT 2 cut(s) 2215, 2224
HspAI GCGC 2 cut(s) 1578, 2242
Kpn2I TCCGGA 1 cut(s) 2109
Ksp22I TGATCA 2 cut(s) 31, 1885
LmnI GCTCC 7 cut(s) 139, 1336, 1457, 1607, 1615, 1616, 2112
Lsp1109I GCAGC 9 cut(s) 985, 1097, 1329, 1351, 1447, 1568, 1724, 1839, 2038
LweI GCATC 4 cut(s) 1281, 1300, 1724, 1753
MaeI CTAG 7 cut(s) 9, 665, 1002, 1242, 1670, 2265, 2373
MaeII ACGT 2 cut(s) 2215, 2224
MflI RGATCY 2 cut(s) 782, 799
MhlI GDGCHC 6 cut(s) 195, 754, 1612, 1732, 1854, 1996
MlyI GAGTC 5 cut(s) 516, 584, 898, 1080, 1515
MmeI TCCRAC 2 cut(s) 259, 2198
MroI TCCGGA 1 cut(s) 2109
MroXI GAANNNNTTC 1 cut(s) 2435
MslI CAYNNNNRTG 2 cut(s) 2492, 2600
MspA1I CMGCKG 3 cut(s) 947, 1342, 1664
MspCI CTTAAG 2 cut(s) 755, 1414
MspI CCGG 2 cut(s) 397, 2110
MspR9I CCNGG 8 cut(s) 398, 536, 616, 797, 1186, 1614, 2153, 2585
MvaI CCWGG 7 cut(s) 536, 616, 797, 1186, 1614, 2153, 2585
MvnI CGCG 2 cut(s) 1578, 2244
MwoI GCNNNNNNNGC 4 cut(s) 1575, 1616, 1670, 1772
NciI CCSGG 1 cut(s) 398
NcoI CCATGG 2 cut(s) 313, 2385
NdeI CATATG 3 cut(s) 552, 687, 841
NlaIV GGNNCC 1 cut(s) 540
NmuCI GTSAC 3 cut(s) 2016, 2030, 2147
NspI RCATGY 4 cut(s) 146, 1026, 1044, 2042
PacI TTAATTAA 1 cut(s) 564
PaeI GCATGC 1 cut(s) 146
PaeR7I CTCGAG 1 cut(s) 501
PagI TCATGA 1 cut(s) 1372
PaqCI CACCTGC 1 cut(s) 2506
PasI CCCWGGG 2 cut(s) 535, 1185
PciI ACATGT 1 cut(s) 1022
PcsI WCGNNNNNNNCGW 1 cut(s) 2221
PdmI GAANNNNTTC 1 cut(s) 2435
PfeI GAWTC 8 cut(s) 43, 112, 628, 879, 1006, 2395, 2446, 2483
PflFI GACNNNGTC 2 cut(s) 21, 1478
PflMI CCANNNNNTGG 1 cut(s) 161
PfoI TCCNGGA 3 cut(s) 795, 1612, 2583
PleI GAGTC 5 cut(s) 515, 583, 897, 1079, 1514
PpsI GAGTC 5 cut(s) 515, 583, 897, 1079, 1514
PscI ACATGT 1 cut(s) 1022
PsiI TTATAA 2 cut(s) 219, 2502
Psp124BI GAGCTC 2 cut(s) 1612, 1854
Psp6I CCWGG 7 cut(s) 534, 614, 795, 1184, 1612, 2151, 2583
PspEI GGTNACC 1 cut(s) 2147
PspGI CCWGG 7 cut(s) 534, 614, 795, 1184, 1612, 2151, 2583
PspN4I GGNNCC 1 cut(s) 540
PspPI GGNCC 4 cut(s) 388, 539, 703, 2275
PspXI VCTCGAGB 1 cut(s) 501
PstI CTGCAG 1 cut(s) 1777
PsuI RGATCY 2 cut(s) 782, 799
PsyI GACNNNGTC 2 cut(s) 21, 1478
PvuII CAGCTG 2 cut(s) 1342, 1664
RsaI GTAC 6 cut(s) 490, 1067, 1422, 1636, 1800, 1929
RsaNI GTAC 6 cut(s) 489, 1066, 1421, 1635, 1799, 1928
RseI CAYNNNNRTG 2 cut(s) 2492, 2600
SacI GAGCTC 2 cut(s) 1612, 1854
Sau96I GGNCC 4 cut(s) 388, 539, 703, 2275
ScaI AGTACT 1 cut(s) 490
SchI GAGTC 5 cut(s) 516, 584, 898, 1080, 1515
ScrFI CCNGG 8 cut(s) 398, 536, 616, 797, 1186, 1614, 2153, 2585
SduI GDGCHC 6 cut(s) 195, 754, 1612, 1732, 1854, 1996
SfaNI GCATC 4 cut(s) 1281, 1300, 1724, 1753
SfcI CTRYAG 2 cut(s) 717, 1773
Sfr274I CTCGAG 1 cut(s) 501
SinI GGWCC 2 cut(s) 388, 703
SlaI CTCGAG 1 cut(s) 501
SmiMI CAYNNNNRTG 2 cut(s) 2492, 2600
SmlI CTYRAG 5 cut(s) 272, 501, 755, 1414, 2299
SmoI CTYRAG 5 cut(s) 272, 501, 755, 1414, 2299
SphI GCATGC 1 cut(s) 146
SsiI CCGC 6 cut(s) 5, 945, 1434, 1576, 1844, 2244
SspI AATATT 2 cut(s) 1059, 2434
SspMI CTAG 7 cut(s) 9, 665, 1002, 1242, 1670, 2265, 2373
SstI GAGCTC 2 cut(s) 1612, 1854
StyD4I CCNGG 8 cut(s) 396, 534, 614, 795, 1184, 1612, 2151, 2583
StyI CCWWGG 4 cut(s) 313, 1320, 1438, 2385
TaaI ACNGT 6 cut(s) 905, 1152, 1203, 1330, 1353, 2427
TaiI ACGT 2 cut(s) 2218, 2227
TaqI TCGA 5 cut(s) 73, 502, 710, 765, 2429
TatI WGTACW 3 cut(s) 488, 1798, 1927
TauI GCSGC 2 cut(s) 8, 1437
TfiI GAWTC 8 cut(s) 43, 112, 628, 879, 1006, 2395, 2446, 2483
TscAI CASTG 5 cut(s) 255, 754, 1930, 2011, 2494
TseFI GTSAC 3 cut(s) 2016, 2030, 2147
TseI GCWGC 9 cut(s) 998, 1110, 1339, 1342, 1460, 1556, 1712, 1827, 2051
Tsp45I GTSAC 3 cut(s) 2016, 2030, 2147
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 5 cut(s) 255, 754, 1930, 2011, 2494
Tth111I GACNNNGTC 2 cut(s) 21, 1478
Van91I CCANNNNNTGG 1 cut(s) 161
Vha464I CTTAAG 2 cut(s) 755, 1414
VpaK11BI GGWCC 2 cut(s) 388, 703
XapI RAATTY 8 cut(s) 93, 102, 122, 331, 2072, 2118, 2127, 2193
XceI RCATGY 4 cut(s) 146, 1026, 1044, 2042
XcmI CCANNNNNNNNNTGG 1 cut(s) 2581
XhoI CTCGAG 1 cut(s) 501
XmnI GAANNNNTTC 1 cut(s) 2435
XspI CTAG 7 cut(s) 9, 665, 1002, 1242, 1670, 2265, 2373
ZrmI AGTACT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.