FvH4_1g20730

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0001949
Physical Location & Seq
Reverse (-)
12880395 .. 12891658
11264 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20730.t1

Sequence Viewer

Length: 3225 bp
ATGGCGGAGACCAAGCGCTATGGTCTTGGGAATCAATTGGATATTCAGCAAATACTCTTAGAAGCTAAGCATCGATGGCTACGTCCAGCTGAAATATGTGAAATTCTTCAGAATTATAAAAAATTTCATATATCTACAGAGCCTGCAAGTACGCCACCTGGTGGATCACTTTTTCTTTTCGATCGAAAGGTGCTGAGGTATTTCAGGAAAGATGGTCATAACTGGAGGAAGAAAAAAGATGGAAAGACAGTGAAGGAGGCCCATGAGAGGCTCAAGGCTGGAAGTGTTGATGTGTTGCACTGTTACTATGCCCACGGAGAAGACAACGAAAATTTTCAAAGACGCAGTTATTGGATGCTTGAAGAGGACCTCTCGCACATTGTTCTCGTCCACTACCGGGAAGTAAAGGGAAATAGGACAAATTTTAATCATGTTAAAGAGACTGAAGGTGTTGCCTATTCCAACGGAGCTGAACAAAGTGCACGCCAGTCTGAGATGGAGAATTCTGTTTCTTCTAGTTTCAATCCAAGTAGTTATCAAATGCATTCTCAAACTACAGAGGCGACAAGTTTGAGTAGTGCACAGGCATCAGAGTTCGAAGATGCGGAATCAGCATTTTATAACCAAGCAAGTTCACGGTTGCAGCCTATGGCTGAGAAGATTAATTCTGAGTTTGCTGATGCGTACTATCCCACCTTTTCAAATGATTTTCAAGAAAAGTTGTCAACCATTCCTGGGGTGGATTTTAGTTCACTCTCTCAAGCATATAAGGGGGAGGATAGTATTCATGCTGGAATAACCCATGAGCCCAGGAAAGATCGTGATTTTGCATTATGGGACGATATGGAAAACAGTGCTACTGGCGTTCAATCTTTTCAGCCATCATTTTCAGCAACACATTCTGATACAATGGGAAGCTTTCCTAAGCAAGAAATTGAGACAATTGGACATCTTTATACAGACAGTTTTGATAAAAGGCTGGTCTATGGGATGGAAAATAGACCAAAGGTCCAACAAAGTTGGCAGACCTCTGAAGGATCATCAAACTGGCCTATGGATCAGAGCATTCAGTCACATGCACAATACAATGTCACCAGTAAGCTCCATGATGGAGCCGATGCCACTGATTTACTGAAGTCATTGGGGCCTTTCCTTATGGATTCAGACAAGCAAAATGACCTTCAATTTCATCTTTCAAACACAGATTCTATTTCAAAGAGAAATGATATCATTGAGGGAAAAGCTGATTACCCTTCTGCTATTAAGCCTCTTTTGGATGGGGCGTTTGGTGATGGACTGAAGAAGCTTGACAGTTTCAATCGATGGATGAGTAAAGAACTGGAAGATGTGGATGAGCCACAAATGCAGTCCAGTTCTGGGGCCTACTGGGAGACTGTTGAAAGTGAGAATGAGGTTGACGAGTCCAGTGTTCCCCTGCAAGTACGCTTGGATAGTTATATGCTGGGGCCTTCTCTTTCTCATGACCAGCTCTTTAGCATTGTTGATTTCTCACCAAGCTGGGCATATGAAAACTCTGAAATCAAGGTTTTGATCACAGGAAGATTTTTGAAAAGTCAACATGCAGAAAGCTGTAAATGGTCTTGTATGTTTGGGGAAGTTGAAGTTCCTGCTGAGGTTATAGCAGATGGTGTTCTTCGTTGTTATACTCCCATACACAAGGCTGGAAGGGTTCCTTTCTATGTGACATGTTCCAATAGGCTGGCTTGTAGTGAAGTAAGGGAGTTCGAATACCGAGTTGCCGAAACTCAAGATGTTGATTGTAAAGATTATTACAGTGATTTCTCAAATGAAACACTCAGTATGCGCTTTGGAAATTTTTTGACTTTAAGCTCTACATCTCCAAATTGTGATCCAGCAAGTATTGCAGAGAATTCTGAGGTGAACAGTAAAATTACTTCTCTGCTGAAAAATGACAATGATGAATGGGACAAGATGCTACAACTTACGTCAGACGAGGACTTTTCCTTGAAGAGAGTGGAGGAACAGCTACATCAACAGTTACTTAAAGAGAAGTTGCATGCATGGCTCTTGCAAAAGCTGGCTGCTGGTGGTAAAGGCCCTAATGTTCTAGATGAGGGTGGCCAAGGTGTGTTACATTTTGGAGCCGCGCTTGGCTATGACTGGGTTCTATTGCCCACAATAACTGCTGGAGTGAGCGTCAATTTTCGTGATGTGAATGGATGGACAGCACTTCATTGGGCAGCCTTTTGTGGCAGGGAGCGCACAGTTGCTTCTCTCATCTCCCTTGGTGCAGCTCCTGGAGCATTAACAGATCCAACTGCCAAATATCCTTCAGGAGAAACACCTGCAGACCTAGCTTCTGAACAAGGACACAAAGGGATTGCTGGTTATCTTGCAGAATCTGCTTTGAGTAAACACCTTGAATCTCTTAATTTGGATATTAAGGACGGAAATTCTGCAGAGATATCAGGAGCAAAAGCAGTTTCAGGTTCTAGCAGGGATGGCGAGTTGACAGATGGGCTTTCCCTGAGGGATTCGTTAACTGCTGTCTGTAATGCGACACAAGCTGCTGCTCGTATTCATCAAGTATTCAGGGTACAATCATTCCAAAGAAAGCAGTTGAAAGAGTACGGTGGTGATAAATTTGGAATTTCAAATGAAAGGGCACTATCACTTATTGCAGTCAAGTCGCATAAGGCTGGGAAACGTGATGAGCATGTGGATGCTGCAGCAGTACGAATACAAAATAAGTTCCGTAGTTGGAAGGGTAGAAAAGACTTTCTGATAATCCGACAACGGATTGTCAAAATTCAGGCCCATGTAAGAGGTCACCAGGTGAGGAAAAACTATAAAAAGATAGTCTGGACCGTAGGAATTGTAGAGAAGATCATCCTGCGTTGGAGAAGGAAAGGAAGTGGGCTGCGCGGTTTCAAGCCAGAACCTCTTACTGAGGGACCCAGCATGCAAGTTTCATCCACTAAGGAGGACGATGATGATGTACTGAAAGAAGGAAGAAAGCAAACAGAGGAAAGGATGCAGAAGGCACTTGCTAGGGTGAAATCCATGGCTCAGTATCCTGAAGCAAGAGATCAATATCGTCGACTTTTAAATGTTGTCACTGAGATTCAGGAAACAAAGGTGCTGAATAGTTCAGAAGGGACATCAGCGTATATGGATGATGACCTCATAGACATAGAAGCGTTGTTCGATGATGACGTTTTCATGCCTACAGCTACTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

1075

Amino Acids

120.64

Weight (kDa)

5.58

Isoelectric Point (pI)

41.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 21 - 134 2.7e-50 CG-1 domain
TIG PF01833 500 - 576 8.4e-06 IPT/TIG domain
Ank_2 PF12796 707 - 796 8e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 621
AarI CACCTGC 1 cut(s) 2335
Acc36I ACCTGC 1 cut(s) 2335
AccB7I CCANNNNNTGG 1 cut(s) 161
AccI GTMKAC 1 cut(s) 3082
AccII CGCG 2 cut(s) 2129, 2907
AciI CCGC 4 cut(s) 5, 605, 2127, 2907
AclWI GGATC 5 cut(s) 172, 1045, 1065, 1865, 2288
AcoI YGGCCR 1 cut(s) 2101
AcuI CTGAAG 7 cut(s) 92, 465, 1053, 1154, 1319, 2298, 3081
AdeI CACNNNGTG 2 cut(s) 161, 2818
AfaI GTAC 7 cut(s) 151, 686, 1443, 2580, 2612, 2718, 2982
AfeI AGCGCT 1 cut(s) 17
AfiI CCNNNNNNNGG 5 cut(s) 161, 267, 397, 735, 1377
AflIII ACRYGT 1 cut(s) 1706
AhdI GACNNNNNGTC 1 cut(s) 1007
AjnI CCWGG 5 cut(s) 157, 733, 809, 2278, 2814
Alw21I GWGCWC 2 cut(s) 484, 583
Alw26I GTCTC 4 cut(s) 2, 434, 932, 1385
Alw44I GTGCAC 2 cut(s) 480, 579
AlwI GGATC 5 cut(s) 172, 1045, 1065, 1865, 2288
AlwNI CAGNNNCTG 3 cut(s) 143, 2279, 2384
Aor51HI AGCGCT 1 cut(s) 17
AoxI GGCC 8 cut(s) 258, 1049, 1145, 1380, 1466, 2077, 2101, 2796
ApaLI GTGCAC 2 cut(s) 480, 579
ApeKI GCWGC 9 cut(s) 643, 2063, 2222, 2273, 2549, 2552, 2708, 2711, 2902
AseI ATTAAT 1 cut(s) 663
Asp700I GAANNNNTTC 2 cut(s) 105, 333
AspLEI GCGC 5 cut(s) 18, 1827, 2131, 2244, 2907
AsuC2I CCSGG 1 cut(s) 398
AsuHPI GGTGA 8 cut(s) 1084, 1301, 1503, 1912, 2630, 2804, 2830, 3049
AsuII TTCGAA 2 cut(s) 597, 1746
AvaII GGWCC 4 cut(s) 367, 1009, 2847, 2936
AxyI CCTNAGG 1 cut(s) 2510
BaeGI GKGCMC 3 cut(s) 484, 583, 2650
BalI TGGCCA 1 cut(s) 2103
BanII GRGCYC 1 cut(s) 810
BarI GAAGNNNNNNTAC 2 cut(s) 1900, 1932
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 2 cut(s) 484, 583
BbvCI CCTCAGC 2 cut(s) 194, 1632
BbvI GCAGC 9 cut(s) 655, 2050, 2234, 2285, 2536, 2539, 2695, 2723, 2889
BcgI CGANNNNNNTGC 2 cut(s) 2652, 2686
BciT130I CCWGG 5 cut(s) 159, 735, 811, 2280, 2816
BciVI GTATCC 1 cut(s) 3066
BclI TGATCA 1 cut(s) 1551
BcnI CCSGG 1 cut(s) 398
BcoDI GTCTC 4 cut(s) 2, 434, 932, 1385
BfaI CTAG 5 cut(s) 516, 2090, 2336, 2475, 3033
BfmI CTRYAG 6 cut(s) 135, 555, 2328, 2439, 2709, 3210
BfoI RGCGCY 1 cut(s) 19
BfuAI ACCTGC 1 cut(s) 2335
BfuI GTATCC 1 cut(s) 3066
BlpI GCTNAGC 1 cut(s) 66
Bme1390I CCNGG 6 cut(s) 159, 398, 735, 811, 2280, 2816
Bme18I GGWCC 4 cut(s) 367, 1009, 2847, 2936
BmeRI GACNNNNNGTC 1 cut(s) 1007
BmiI GGNNCC 8 cut(s) 1114, 1146, 1381, 1467, 1692, 2125, 2937, 2938
BmrFI CCNGG 6 cut(s) 159, 398, 735, 811, 2280, 2816
BmrI ACTGGG 2 cut(s) 1396, 2152
BmsI GCATC 9 cut(s) 79, 345, 592, 596, 670, 1108, 1944, 2695, 3006
BmuI ACTGGG 2 cut(s) 1396, 2152
BpiI GAAGAC 1 cut(s) 327
BplI GAGNNNNNCTC 2 cut(s) 356, 388
BpmI CTGGAG 3 cut(s) 244, 2190, 2301
Bpu10I CCTNAGC 3 cut(s) 194, 924, 1632
Bpu1102I GCTNAGC 1 cut(s) 66
Bpu14I TTCGAA 2 cut(s) 597, 1746
BpuEI CTTGAG 3 cut(s) 257, 744, 1752
BpuMI CCSGG 1 cut(s) 398
Bsa29I ATCGAT 2 cut(s) 73, 1321
BsaBI GATNNNNATC 1 cut(s) 3075
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 6 cut(s) 313, 734, 809, 2104, 2266, 3045
BsaXI ACNNNNNCTCC 2 cut(s) 2163, 2193
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 267, 397, 735, 1377
Bse21I CCTNAGG 1 cut(s) 2510
Bse8I GATNNNNATC 1 cut(s) 3075
BseBI CCWGG 5 cut(s) 159, 735, 811, 2280, 2816
BseCI ATCGAT 2 cut(s) 73, 1321
BseDI CCNNGG 6 cut(s) 313, 734, 809, 2104, 2266, 3045
BseJI GATNNNNATC 1 cut(s) 3075
BseLI CCNNNNNNNGG 5 cut(s) 161, 267, 397, 735, 1377
BseSI GKGCMC 3 cut(s) 484, 583, 2650
BseXI GCAGC 9 cut(s) 655, 2050, 2234, 2285, 2536, 2539, 2695, 2723, 2889
BseYI CCCAGC 4 cut(s) 1462, 1518, 2681, 2939
BsgI GTGCAG 1 cut(s) 2292
Bsh1236I CGCG 2 cut(s) 2129, 2907
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 8 cut(s) 260, 1051, 1147, 1382, 1468, 2079, 2103, 2798
BshVI ATCGAT 2 cut(s) 73, 1321
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 2 cut(s) 484, 583
BsiSI CCGG 1 cut(s) 397
BslFI GGGAC 4 cut(s) 851, 1961, 2949, 3154
BslI CCNNNNNNNGG 5 cut(s) 161, 267, 397, 735, 1377
BsmAI GTCTC 4 cut(s) 2, 434, 932, 1385
BsmFI GGGAC 4 cut(s) 851, 1961, 2949, 3154
BsmI GAATGC 2 cut(s) 544, 1065
BsnI GGCC 8 cut(s) 260, 1051, 1147, 1382, 1468, 2079, 2103, 2798
Bso31I GGTCTC 1 cut(s) 2
Bsp119I TTCGAA 2 cut(s) 597, 1746
Bsp1286I GDGCHC 4 cut(s) 484, 583, 810, 2650
Bsp1720I GCTNAGC 1 cut(s) 66
Bsp19I CCATGG 1 cut(s) 3045
BspACI CCGC 4 cut(s) 5, 605, 2127, 2907
BspANI GGCC 8 cut(s) 260, 1051, 1147, 1382, 1468, 2079, 2103, 2798
BspDI ATCGAT 2 cut(s) 73, 1321
BspFNI CGCG 2 cut(s) 2129, 2907
BspHI TCATGA 1 cut(s) 1480
BspLI GGNNCC 8 cut(s) 1114, 1146, 1381, 1467, 1692, 2125, 2937, 2938
BspMAI CTGCAG 3 cut(s) 2332, 2443, 2713
BspMI ACCTGC 1 cut(s) 2335
BspPI GGATC 5 cut(s) 172, 1045, 1065, 1865, 2288
BspT104I TTCGAA 2 cut(s) 597, 1746
BspTNI GGTCTC 1 cut(s) 2
BssECI CCNNGG 6 cut(s) 313, 734, 809, 2104, 2266, 3045
BssT1I CCWWGG 3 cut(s) 2104, 2266, 3045
Bst2UI CCWGG 5 cut(s) 159, 735, 811, 2280, 2816
Bst6I CTCTTC 2 cut(s) 357, 1985
BstAPI GCANNNNNTGC 2 cut(s) 1883, 2384
BstBI TTCGAA 2 cut(s) 597, 1746
BstC8I GCNNGC 6 cut(s) 144, 484, 1722, 2040, 2061, 2945
BstDSI CCRYGG 2 cut(s) 313, 3045
BstEII GGTNACC 1 cut(s) 2810
BstFNI CGCG 2 cut(s) 2129, 2907
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 5 cut(s) 18, 1827, 2131, 2244, 2907
BstMAI GTCTC 4 cut(s) 2, 434, 932, 1385
BstMCI CGRYCG 1 cut(s) 184
BstNI CCWGG 5 cut(s) 159, 735, 811, 2280, 2816
BstNSI RCATGY 6 cut(s) 1079, 1583, 1710, 2042, 2702, 2947
BstPI GGTNACC 1 cut(s) 2810
BstSCI CCNGG 6 cut(s) 157, 396, 733, 809, 2278, 2814
BstSFI CTRYAG 6 cut(s) 135, 555, 2328, 2439, 2709, 3210
BstSLI GKGCMC 3 cut(s) 484, 583, 2650
BstUI CGCG 2 cut(s) 2129, 2907
BstV1I GCAGC 9 cut(s) 655, 2050, 2234, 2285, 2536, 2539, 2695, 2723, 2889
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 1 cut(s) 2293
BstXI CCANNNNNNTGG 1 cut(s) 1720
BstYI RGATCY 1 cut(s) 2293
Bsu15I ATCGAT 2 cut(s) 73, 1321
Bsu36I CCTNAGG 1 cut(s) 2510
BsuI GTATCC 1 cut(s) 3066
BsuRI GGCC 8 cut(s) 260, 1051, 1147, 1382, 1468, 2079, 2103, 2798
BsuTUI ATCGAT 2 cut(s) 73, 1321
BtgI CCRYGG 2 cut(s) 313, 3045
BtsIMutI CAGTG 7 cut(s) 255, 298, 859, 1122, 1432, 1801, 3099
BveI ACCTGC 1 cut(s) 2335
Cac8I GCNNGC 6 cut(s) 144, 484, 1722, 2040, 2061, 2945
CaiI CAGNNNCTG 3 cut(s) 143, 2279, 2384
CciI TCATGA 1 cut(s) 1480
CfoI GCGC 5 cut(s) 18, 1827, 2131, 2244, 2907
ClaI ATCGAT 2 cut(s) 73, 1321
CseI GACGC 2 cut(s) 351, 2167
CsiI ACCWGGT 2 cut(s) 157, 2814
Csp6I GTAC 7 cut(s) 150, 685, 1442, 2579, 2611, 2717, 2981
CviQI GTAC 7 cut(s) 150, 685, 1442, 2579, 2611, 2717, 2981
DraI TTTAAA 1 cut(s) 3090
DraIII CACNNNGTG 2 cut(s) 161, 2818
DriI GACNNNNNGTC 1 cut(s) 1007
EaeI YGGCCR 1 cut(s) 2101
Eam1104I CTCTTC 2 cut(s) 357, 1985
Eam1105I GACNNNNNGTC 1 cut(s) 1007
EarI CTCTTC 2 cut(s) 357, 1985
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 3 cut(s) 2104, 2266, 3045
Eco24I GRGCYC 1 cut(s) 810
Eco31I GGTCTC 1 cut(s) 2
Eco32I GATATC 2 cut(s) 1228, 2448
Eco47I GGWCC 4 cut(s) 367, 1009, 2847, 2936
Eco47III AGCGCT 1 cut(s) 17
Eco57I CTGAAG 7 cut(s) 92, 465, 1053, 1154, 1319, 2298, 3081
Eco81I CCTNAGG 1 cut(s) 2510
Eco91I GGTNACC 1 cut(s) 2810
EcoO109I RGGNCCY 6 cut(s) 367, 1145, 1380, 1466, 2078, 2936
EcoO65I GGTNACC 1 cut(s) 2810
EcoRI GAATTC 2 cut(s) 502, 1891
EcoRII CCWGG 5 cut(s) 157, 733, 809, 2278, 2814
EcoRV GATATC 2 cut(s) 1228, 2448
EcoT14I CCWWGG 3 cut(s) 2104, 2266, 3045
EcoT22I ATGCAT 2 cut(s) 546, 2044
EcoT38I GRGCYC 1 cut(s) 810
ErhI CCWWGG 3 cut(s) 2104, 2266, 3045
FalI AAGNNNNNCTT 2 cut(s) 1678, 1710
FaqI GGGAC 4 cut(s) 851, 1961, 2949, 3154
FauNDI CATATG 1 cut(s) 1525
FbaI TGATCA 1 cut(s) 1551
FblI GTMKAC 1 cut(s) 3082
FriOI GRGCYC 1 cut(s) 810
FspBI CTAG 5 cut(s) 516, 2090, 2336, 2475, 3033
GlaI GCGC 5 cut(s) 17, 1826, 2130, 2243, 2906
GsaI CCCAGC 4 cut(s) 1466, 1522, 2685, 2943
GsuI CTGGAG 3 cut(s) 244, 2190, 2301
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 8 cut(s) 260, 1051, 1147, 1382, 1468, 2079, 2103, 2798
HapII CCGG 1 cut(s) 397
HgaI GACGC 2 cut(s) 351, 2167
HhaI GCGC 5 cut(s) 18, 1827, 2131, 2244, 2907
Hin6I GCGC 5 cut(s) 16, 1825, 2129, 2242, 2905
HinP1I GCGC 5 cut(s) 16, 1825, 2129, 2242, 2905
HincII GTYRAC 6 cut(s) 726, 1417, 1577, 2493, 2523, 3083
HindII GTYRAC 6 cut(s) 726, 1417, 1577, 2493, 2523, 3083
HindIII AAGCTT 2 cut(s) 916, 1304
HinfI GANTC 9 cut(s) 31, 608, 1160, 1205, 1421, 2381, 2405, 2516, 3106
HpaI GTTAAC 1 cut(s) 2523
HpaII CCGG 1 cut(s) 397
HphI GGTGA 8 cut(s) 1084, 1301, 1503, 1912, 2630, 2804, 2830, 3049
Hpy99I CGWCG 1 cut(s) 3084
HpyCH4IV ACGT 4 cut(s) 82, 1967, 2689, 3198
HpySE526I ACGT 4 cut(s) 82, 1967, 2689, 3198
HspAI GCGC 5 cut(s) 16, 1825, 2129, 2242, 2905
KflI GGGWCCC 1 cut(s) 2936
Ksp22I TGATCA 1 cut(s) 1551
KspAI GTTAAC 1 cut(s) 2523
LmnI GCTCC 8 cut(s) 467, 1107, 1112, 2123, 2239, 2281, 2282, 2453
Lsp1109I GCAGC 9 cut(s) 655, 2050, 2234, 2285, 2536, 2539, 2695, 2723, 2889
LweI GCATC 9 cut(s) 79, 345, 592, 596, 670, 1108, 1944, 2695, 3006
MabI ACCWGGT 2 cut(s) 157, 2814
MaeI CTAG 5 cut(s) 516, 2090, 2336, 2475, 3033
MaeII ACGT 4 cut(s) 82, 1967, 2689, 3198
MaeIII GTNAC 8 cut(s) 302, 1071, 1090, 1702, 2019, 2112, 2810, 3097
MfeI CAATTG 2 cut(s) 35, 942
MflI RGATCY 1 cut(s) 2293
MhlI GDGCHC 4 cut(s) 484, 583, 810, 2650
MlsI TGGCCA 1 cut(s) 2103
MluNI TGGCCA 1 cut(s) 2103
MlyI GAGTC 1 cut(s) 1430
MmeI TCCRAC 6 cut(s) 486, 1036, 2321, 2723, 2797, 2861
Mox20I TGGCCA 1 cut(s) 2103
Mph1103I ATGCAT 2 cut(s) 546, 2044
MroXI GAANNNNTTC 2 cut(s) 105, 333
MscI TGGCCA 1 cut(s) 2103
MslI CAYNNNNRTG 1 cut(s) 2703
Msp20I TGGCCA 1 cut(s) 2103
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 1 cut(s) 397
MspR9I CCNGG 6 cut(s) 159, 398, 735, 811, 2280, 2816
MunI CAATTG 2 cut(s) 35, 942
Mva1269I GAATGC 2 cut(s) 544, 1065
MvaI CCWGG 5 cut(s) 159, 735, 811, 2280, 2816
MvnI CGCG 2 cut(s) 2129, 2907
NciI CCSGG 1 cut(s) 398
NcoI CCATGG 1 cut(s) 3045
NdeI CATATG 1 cut(s) 1525
NlaIV GGNNCC 8 cut(s) 1114, 1146, 1381, 1467, 1692, 2125, 2937, 2938
NmuCI GTSAC 5 cut(s) 1071, 1090, 1702, 2810, 3097
NsiI ATGCAT 2 cut(s) 546, 2044
NspI RCATGY 6 cut(s) 1079, 1583, 1710, 2042, 2702, 2947
NspV TTCGAA 2 cut(s) 597, 1746
PaeI GCATGC 2 cut(s) 2042, 2947
PagI TCATGA 1 cut(s) 1480
PaqCI CACCTGC 1 cut(s) 2335
PciI ACATGT 1 cut(s) 1706
PcsI WCGNNNNNNNCGW 2 cut(s) 79, 3195
PctI GAATGC 2 cut(s) 544, 1065
PdmI GAANNNNTTC 2 cut(s) 105, 333
PfeI GAWTC 8 cut(s) 31, 608, 1160, 1205, 2381, 2405, 2516, 3106
PflMI CCANNNNNTGG 1 cut(s) 161
PfoI TCCNGGA 1 cut(s) 2278
Ple19I CGATCG 1 cut(s) 184
PleI GAGTC 1 cut(s) 1429
PpsI GAGTC 1 cut(s) 1429
PpuMI RGGWCCY 2 cut(s) 367, 2936
PscI ACATGT 1 cut(s) 1706
PshBI ATTAAT 1 cut(s) 663
PsiI TTATAA 2 cut(s) 117, 621
Psp5II RGGWCCY 2 cut(s) 367, 2936
Psp6I CCWGG 5 cut(s) 157, 733, 809, 2278, 2814
PspEI GGTNACC 1 cut(s) 2810
PspFI CCCAGC 4 cut(s) 1462, 1518, 2681, 2939
PspGI CCWGG 5 cut(s) 157, 733, 809, 2278, 2814
PspN4I GGNNCC 8 cut(s) 1114, 1146, 1381, 1467, 1692, 2125, 2937, 2938
PspPPI RGGWCCY 2 cut(s) 367, 2936
PstI CTGCAG 3 cut(s) 2332, 2443, 2713
PstNI CAGNNNCTG 3 cut(s) 143, 2279, 2384
PsuI RGATCY 1 cut(s) 2293
PvuI CGATCG 1 cut(s) 184
PvuII CAGCTG 1 cut(s) 89
RsaI GTAC 7 cut(s) 151, 686, 1443, 2580, 2612, 2718, 2982
RsaNI GTAC 7 cut(s) 150, 685, 1442, 2579, 2611, 2717, 2981
RseI CAYNNNNRTG 1 cut(s) 2703
SalI GTCGAC 1 cut(s) 3081
SchI GAGTC 1 cut(s) 1430
ScrFI CCNGG 6 cut(s) 159, 398, 735, 811, 2280, 2816
SduI GDGCHC 4 cut(s) 484, 583, 810, 2650
SexAI ACCWGGT 2 cut(s) 157, 2814
SfaNI GCATC 9 cut(s) 79, 345, 592, 596, 670, 1108, 1944, 2695, 3006
SfcI CTRYAG 6 cut(s) 135, 555, 2328, 2439, 2709, 3210
SfuI TTCGAA 2 cut(s) 597, 1746
SinI GGWCC 4 cut(s) 367, 1009, 2847, 2936
SmiMI CAYNNNNRTG 1 cut(s) 2703
SmlI CTYRAG 3 cut(s) 272, 759, 1767
SmoI CTYRAG 3 cut(s) 272, 759, 1767
SphI GCATGC 2 cut(s) 2042, 2947
SsiI CCGC 4 cut(s) 5, 605, 2127, 2907
SspMI CTAG 5 cut(s) 516, 2090, 2336, 2475, 3033
StyD4I CCNGG 6 cut(s) 157, 396, 733, 809, 2278, 2814
StyI CCWWGG 3 cut(s) 2104, 2266, 3045
TaiI ACGT 4 cut(s) 85, 1970, 2692, 3201
TaqI TCGA 8 cut(s) 73, 180, 184, 597, 1321, 1746, 3082, 3189
TatI WGTACW 1 cut(s) 2980
TauI GCSGC 1 cut(s) 2129
TfiI GAWTC 8 cut(s) 31, 608, 1160, 1205, 2381, 2405, 2516, 3106
TscAI CASTG 7 cut(s) 255, 305, 859, 1129, 1432, 1801, 3106
TseFI GTSAC 5 cut(s) 1071, 1090, 1702, 2810, 3097
TseI GCWGC 9 cut(s) 643, 2063, 2222, 2273, 2549, 2552, 2708, 2711, 2902
Tsp45I GTSAC 5 cut(s) 1071, 1090, 1702, 2810, 3097
TspGWI ACGGA 5 cut(s) 330, 480, 2445, 2726, 2794
TspRI CASTG 7 cut(s) 255, 305, 859, 1129, 1432, 1801, 3106
Van91I CCANNNNNTGG 1 cut(s) 161
VneI GTGCAC 2 cut(s) 480, 579
VpaK11BI GGWCC 4 cut(s) 367, 1009, 2847, 2936
VspI ATTAAT 1 cut(s) 663
XbaI TCTAGA 1 cut(s) 2089
XceI RCATGY 6 cut(s) 1079, 1583, 1710, 2042, 2702, 2947
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmiI GTMKAC 1 cut(s) 3082
XmnI GAANNNNTTC 2 cut(s) 105, 333
XspI CTAG 5 cut(s) 516, 2090, 2336, 2475, 3033
Zsp2I ATGCAT 2 cut(s) 546, 2044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.