pycom12g03400

proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0001949
Physical Location & Seq
Forward (+)
3174097 .. 3177545
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03400.4

Sequence Viewer

Length: 747 bp
ATGGCGGAGAGGAATCACAATCATCTTGATCAACTTGTTCTTCATTTGAAAGCAAGTAGAATACTTTTTAAAGGGAAACATCGATGGCTACGGGCGGATGAAATATGTGAACTTCTTCGAAATCCTAACGGAATTACAATTTCTGAGGAGCCTACAAATATGCCATCGAGTGGATCCATTTTATTTTTTGATAAGAAGGTGCAGAGACATTTTAGAAAAGATGGCTATAAATGGAAGAAGGCAAAAGATGGAAAGATTGTTAAGGAAGCCCATGAGAGGCTCAAGGTTGGAGGTGTTGATACATTGCACTGCTACTATGCTCATGGAGAAGAAGATGAAAATTTTCAACGACGCTGTTACTGGATGCTTGAAGAGAATCTCTCACATATGGTTCTTGTCCATTATCGGGATGTAAAGGGAGATAAAATAAAGGTTAGAGACATTAAAGAGACTGCAGAAGGTGTTCAACGGTCCCGCGAAACTGACGAAATTACACCCAAATCTGAGATTGACAGTTTTGCTTCTTCTAGTTTCAATCCAAGTAATTTCCAGCATTCACAAGCTACAGATGGGACACCCTTAAATAGTGCTCAAACAGCACAGTCCAAAAACACTGACTCAGGTGTTTGTTTTTTACATTGTCAAAATCTTTCTCCGATATTTGGCACGAGTTCCTTTTCTTTTACAGTTATTTCGGTGATATGTTTTTATTTTTGTTTATGTTCTATTATTGTTGTCTTCGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 CG-1 LRR_3 NB-ARC TIR

Protein Analysis

249

Amino Acids

28.42

Weight (kDa)

8.09

Isoelectric Point (pI)

37.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 170
AccII CGCG 1 cut(s) 477
AciI CCGC 3 cut(s) 5, 95, 475
AclWI GGATC 2 cut(s) 168, 181
AcsI RAATTY 1 cut(s) 340
AfiI CCNNNNNNNGG 4 cut(s) 170, 276, 406, 662
AgsI TTSAA 5 cut(s) 49, 347, 371, 467, 535
AluBI AGCT 1 cut(s) 563
AluI AGCT 1 cut(s) 563
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 3 cut(s) 199, 432, 443
AlwI GGATC 2 cut(s) 168, 181
ApoI RAATTY 1 cut(s) 340
Asp700I GAANNNNTTC 3 cut(s) 114, 342, 462
AspS9I GGNCC 1 cut(s) 471
AsuHPI GGTGA 1 cut(s) 709
AsuII TTCGAA 1 cut(s) 118
AvaII GGWCC 1 cut(s) 471
BamHI GGATCC 1 cut(s) 173
BauI CACGAG 1 cut(s) 667
BbsI GAAGAC 1 cut(s) 730
Bbv12I GWGCWC 1 cut(s) 592
BccI CCATC 5 cut(s) 78, 172, 215, 242, 563
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 199, 432, 443
BfaI CTAG 1 cut(s) 528
BfmI CTRYAG 2 cut(s) 453, 564
Bme18I GGWCC 1 cut(s) 471
BmgT120I GGNCC 1 cut(s) 471
BmiI GGNNCC 3 cut(s) 150, 175, 473
BmsI GCATC 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 730
BplI GAGNNNNNCTC 2 cut(s) 365, 397
Bpu14I TTCGAA 1 cut(s) 118
BpuEI CTTGAG 1 cut(s) 266
Bsa29I ATCGAT 1 cut(s) 82
Bsc4I CCNNNNNNNGG 4 cut(s) 170, 276, 406, 662
Bse1I ACTGG 1 cut(s) 365
Bse3DI GCAATG 1 cut(s) 302
BseCI ATCGAT 1 cut(s) 82
BseGI GGATG 3 cut(s) 103, 369, 415
BseLI CCNNNNNNNGG 4 cut(s) 170, 276, 406, 662
BseMI GCAATG 1 cut(s) 302
BseMII CTCAG 3 cut(s) 135, 495, 633
BseNI ACTGG 1 cut(s) 365
BseRI GAGGAG 1 cut(s) 161
BsgI GTGCAG 1 cut(s) 221
Bsh1236I CGCG 1 cut(s) 477
BshVI ATCGAT 1 cut(s) 82
BsiHKAI GWGCWC 1 cut(s) 592
BslFI GGGAC 2 cut(s) 457, 586
BslI CCNNNNNNNGG 4 cut(s) 170, 276, 406, 662
BsmAI GTCTC 3 cut(s) 199, 432, 443
BsmFI GGGAC 2 cut(s) 457, 586
BsmI GAATGC 1 cut(s) 553
Bsp119I TTCGAA 1 cut(s) 118
Bsp1286I GDGCHC 1 cut(s) 592
Bsp143I GATC 2 cut(s) 28, 173
BspACI CCGC 3 cut(s) 5, 95, 475
BspCNI CTCAG 3 cut(s) 136, 496, 632
BspDI ATCGAT 1 cut(s) 82
BspFNI CGCG 1 cut(s) 477
BspLI GGNNCC 3 cut(s) 150, 175, 473
BspMAI CTGCAG 1 cut(s) 457
BspPI GGATC 2 cut(s) 168, 181
BspT104I TTCGAA 1 cut(s) 118
BsrDI GCAATG 1 cut(s) 302
BsrI ACTGG 1 cut(s) 365
BssMI GATC 2 cut(s) 28, 173
BssSI CACGAG 1 cut(s) 667
Bst2BI CACGAG 1 cut(s) 667
Bst4CI ACNGT 4 cut(s) 471, 515, 603, 688
Bst6I CTCTTC 1 cut(s) 366
BstBI TTCGAA 1 cut(s) 118
BstDEI CTNAG 3 cut(s) 144, 504, 619
BstF5I GGATG 3 cut(s) 103, 369, 415
BstFNI CGCG 1 cut(s) 477
BstKTI GATC 2 cut(s) 31, 176
BstMAI GTCTC 3 cut(s) 199, 432, 443
BstMBI GATC 2 cut(s) 28, 173
BstMWI GCNNNNNNNGC 1 cut(s) 596
BstSFI CTRYAG 2 cut(s) 453, 564
BstUI CGCG 1 cut(s) 477
BstV2I GAAGAC 1 cut(s) 730
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
Bsu15I ATCGAT 1 cut(s) 82
BsuTUI ATCGAT 1 cut(s) 82
BtsCI GGATG 3 cut(s) 103, 369, 415
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 2 cut(s) 307, 612
Cfr13I GGNCC 1 cut(s) 471
ClaI ATCGAT 1 cut(s) 82
CseI GACGC 1 cut(s) 360
CviAII CATG 2 cut(s) 272, 323
CviJI RGCY 6 cut(s) 88, 151, 225, 269, 280, 563
CviKI_1 RGCY 6 cut(s) 88, 151, 225, 269, 280, 563
DdeI CTNAG 3 cut(s) 144, 504, 619
DpnI GATC 2 cut(s) 30, 175
DpnII GATC 2 cut(s) 28, 173
DraI TTTAAA 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
EciI GGCGGA 2 cut(s) 20, 110
Eco47I GGWCC 1 cut(s) 471
FaeI CATG 2 cut(s) 275, 326
FaqI GGGAC 2 cut(s) 457, 586
FatI CATG 2 cut(s) 271, 322
FauI CCCGC 1 cut(s) 482
FauNDI CATATG 1 cut(s) 387
FbaI TGATCA 1 cut(s) 28
FokI GGATG 3 cut(s) 110, 376, 422
FspBI CTAG 1 cut(s) 528
HgaI GACGC 1 cut(s) 360
Hin1II CATG 2 cut(s) 275, 326
HinfI GANTC 3 cut(s) 13, 376, 617
HphI GGTGA 1 cut(s) 709
Hpy166II GTNNAC 1 cut(s) 110
Hpy188I TCNGA 3 cut(s) 145, 505, 657
Hpy188III TCNNGA 2 cut(s) 26, 407
Hpy8I GTNNAC 1 cut(s) 110
Hpy99I CGWCG 1 cut(s) 354
HpyAV CCTTC 3 cut(s) 190, 232, 452
HpyCH4III ACNGT 4 cut(s) 471, 515, 603, 688
HpyCH4V TGCA 3 cut(s) 202, 307, 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 596
HpyF3I CTNAG 3 cut(s) 144, 504, 619
Hsp92II CATG 2 cut(s) 275, 326
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 2 cut(s) 28, 173
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 3 cut(s) 346, 563, 606
LweI GCATC 1 cut(s) 354
MaeI CTAG 1 cut(s) 528
MaeIII GTNAC 1 cut(s) 356
MalI GATC 2 cut(s) 30, 175
MboI GATC 2 cut(s) 28, 173
MboII GAAGA 8 cut(s) 32, 107, 247, 341, 344, 383, 516, 730
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 592
MluCI AATT 5 cut(s) 132, 138, 340, 489, 544
MlyI GAGTC 1 cut(s) 611
MmeI TCCRAC 1 cut(s) 268
MnlI CCTC 4 cut(s) 3, 139, 270, 284
MroXI GAANNNNTTC 3 cut(s) 114, 342, 462
MseI TTAA 5 cut(s) 69, 261, 444, 581, 745
Mva1269I GAATGC 1 cut(s) 553
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 1 cut(s) 596
NdeI CATATG 1 cut(s) 387
NdeII GATC 2 cut(s) 28, 173
NlaIII CATG 2 cut(s) 275, 326
NlaIV GGNNCC 3 cut(s) 150, 175, 473
NspV TTCGAA 1 cut(s) 118
PctI GAATGC 1 cut(s) 553
PdmI GAANNNNTTC 3 cut(s) 114, 342, 462
PfeI GAWTC 2 cut(s) 13, 376
PflMI CCANNNNNTGG 1 cut(s) 170
PleI GAGTC 1 cut(s) 611
PpsI GAGTC 1 cut(s) 611
PspN4I GGNNCC 3 cut(s) 150, 175, 473
PspPI GGNCC 1 cut(s) 471
PstI CTGCAG 1 cut(s) 457
PsuI RGATCY 1 cut(s) 173
SaqAI TTAA 5 cut(s) 69, 261, 444, 581, 745
Sau3AI GATC 2 cut(s) 28, 173
Sau96I GGNCC 1 cut(s) 471
SchI GAGTC 1 cut(s) 611
SduI GDGCHC 1 cut(s) 592
SetI ASST 7 cut(s) 201, 288, 295, 435, 463, 565, 625
SfaNI GCATC 1 cut(s) 354
SfcI CTRYAG 2 cut(s) 453, 564
SfuI TTCGAA 1 cut(s) 118
SinI GGWCC 1 cut(s) 471
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
Sse9I AATT 5 cut(s) 132, 138, 340, 489, 544
SsiI CCGC 3 cut(s) 5, 95, 475
SspMI CTAG 1 cut(s) 528
TaaI ACNGT 4 cut(s) 471, 515, 603, 688
TaqI TCGA 3 cut(s) 82, 118, 167
TasI AATT 5 cut(s) 132, 138, 340, 489, 544
TfiI GAWTC 2 cut(s) 13, 376
Tru1I TTAA 5 cut(s) 69, 261, 444, 581, 745
Tru9I TTAA 5 cut(s) 69, 261, 444, 581, 745
TscAI CASTG 2 cut(s) 314, 619
TspDTI ATGAA 3 cut(s) 32, 114, 351
TspGWI ACGGA 1 cut(s) 144
TspRI CASTG 2 cut(s) 314, 619
Van91I CCANNNNNTGG 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 471
XapI RAATTY 1 cut(s) 340
XmnI GAANNNNTTC 3 cut(s) 114, 342, 462
XspI CTAG 1 cut(s) 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.