Rw2G018820

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001949
Physical Location & Seq
Reverse (-)
24757287 .. 24768418
11132 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018820.1

Sequence Viewer

Length: 2718 bp
ATGGAGAATTCTGTTTCTTCTAGTTTCAATCCAAGTAGTTACCAAATGCGTTCTCAAGTTACGGACGCGACAAGCTTGAGTAGTGCTCAGGCATCAGAGTTCGAAGATGTGGAATCAGCATTTTATAACCAAGCAAGCTCCCGGTTGCAGCCTATGGCTGAGAAGATTAATTCTGAGTTCTCTGATGCGTACTATCCCGCATTTTCAAATGATTTTCAAGAAAACTTGGCAACCATTCCTGGGGTGGATTTTAGCTCACTCTCTCAAGCATATAAGAGGGAGGATAGTATTGATGCTGGAGATACCTATGAGCCCAGGAAAGATCGTGAATTTGCATCATGGGATGATATGGAAAACAGTGTTACTGGCATTCAATCTTTTCAGCCATCATTTTCAGCAACACATTCAGATACAATGGGAAGCTTTCCTAAGATTGAGACACTTGGGCATCTTCATACAGACAGTTTTGATAAAAGGCTGGTGTATGGGATTGAAAATAAACCAAAGGTCCAACAAAGTTGGGAGACTTCCGAAGGATCATCAAACTGGCCTGTGGATCAGAGCTTGCAATCACACACAGAATACAATGTCACCGCTAAGCTCGATGAAGGAGCTAATACCACTGATTTACTCAAGTCCTTGGGGCCTTTCCTTATGGATCCAGACAAGCAAAATGACCTTCAATTTCATCTTTCAAACACAGATACTATTTCAAAGAGAAATGATAGCATTGAGAGAAAAGCTGATCACCCTTCTGCTATTAAGCCTCTGTTGGGATTCGATGATGGTCTGAAGAAGCTCGACAGTTTCAACCGATGGATGAGTAAAGAACTTGAAGATGTGGATGAGCCACAAATGCAATCCAGTTCTGGAGCCTACTGGGATACTGTTGAAAGTGAGAATGAGGTTGATGAGTCCAGTGTTCCCCTGCAAGTACGCTTGGATAGCTATATGCTGGGGCCTTCTCTTTCTCATGACCAGCTCTTTAGCATTGTTGATTTCTCACCAAACTGGGCTTATGAAAACTCTGAAATCAAGGTTTTGATCACAGGAAAATTTTTGAAAAGTCAACATGTAGAAAGTTGTAAATGGTCATGTATGTTTGGGGAAGTTGAAGTTCCTGCTGAGGTTATAGCACATGGTGTTCTTCGTTGTTATACTCCCGTACACAAGGCTGGAAGGGTTCCCTTCTATGTTACATGTTCCAATAGGCTGGCTTGTAGTGAAGTGAGGGAATTTGAATACCGAGTCGCCCAAACTCAAGATGTTGATACTAAGGATTATTACAGTGACTGCTCAAATGAAACACTCAATAAGCGCTTCGAAAAATTATTGTCTTTAAGCTCTACATCTTCAAATTCTGATACAGCAAGTACACTAGAGGATTCTGAGTTGAACAGTAAAATTACTTCTATGCTGAAAAGTGACGATGATGAATGGGACAAGATGCTACAACTTATGTCAGTCGATGAATTTTCCTTGGAAAGAGTACAGGAGCAGTTGCTTCAACAGTTACTTAAAGAGAAGTTGCGTGCATGGCTCTTGCAGAAGCTGGCTGGTGGTGGTAAAGGCCCTAGTGTTCTAGACGAGGGTGGCCAAGGTGTGTTACATTTTGGAGCTGCTCTTGGCTATGACTGGGTCTTATTGCCCACAATAACTGCTGGAGTTAGCGTCAATTTTCGTGATGTGAATGGATGGACGGCACTTCATTGGGCAGCTTTTTGTGGCAGAGAGCGCACAGTCGCTTCTCTCATCGCCCTGGGTGCAGCTCCTGGAGCATTAACAGATCCAACTACCAAATATCCTTCAGGAGAAACACCTGCAGACCTAGCTTCTGAACAAGGACACAAAGGAATTGCTGGATATCTTGCAGAATCTGCATTGAGTGCTCACCTTGAATCTCTTAATTTGGATCTCAAGGACGGAAATTCGACAGAGATTTCAGGAACAAAAGCAGTTTCAAGTTCTAGCAGGGATGGGGAGTTAACGGATGGGCTTTCCCTGAGGGATTCTTTAACTGCTGTCTGTAATGCGACACAGGCTGCTGCTCGTATTCATCAAGTGTTCAGGGTACAATCTTTCCAAAGGAAGCAGTTGAAAGAGTATGATGGTGATAAATTTGGACTTTCCAATGAACGGGCACTATCACTTATTGCAGTCAAGTCAAATAAGGCAGGGAAACGTGATGAGCATGTGGATGCTGCAGCAGTACGAATACAAAATAAGTTCCGTAGTTGGAAGGGTAGAAAAGACTATTTGATAATCCGACAAAGGATTGTCAAAATTCAGGCCCATGTAAGAGGTCACCAGGTGAGGAAAAACTATAGAAAGATAGTCTGGTCCGTAGGAATTGTGGAGAAAATCATCCTGCGTTGGAGAAGAAAGGGAAGTGGGTTGCGCGGGTTCAAGCCAGAACCAATTACTGAGGCCCCCAGCATGCAAGTTTCATCCACAAAGGATGATGATTATGACTTGCTGAAAGAAGGAAGGAAGCAAACAGAGGAAAGGTTGCAAAAGGCACTTGCTAGGGTGAAATCCATGGTTCAGTATCCTGAAGCAAGAGATCAATACCATCGACTGTTAAATGTTGTCACTGAGATTCAGGAAACAAAGGTGCTGAATAGTTCAGAGGGGACATCAGCTTATATGGATGATGACCTCATAGACATTGAAGCGTTGTTCGATGATGACATTTTCATGCCTACAGCAGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

905

Amino Acids

101.2

Weight (kDa)

5.09

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 538 - 626 7.5e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 126
AarI CACCTGC 1 cut(s) 1828
Acc36I ACCTGC 1 cut(s) 1828
AccII CGCG 2 cut(s) 68, 2400
AciI CCGC 3 cut(s) 198, 594, 2400
AclWI GGATC 6 cut(s) 544, 564, 653, 666, 1781, 1920
AcoI YGGCCR 1 cut(s) 1594
AcsI RAATTY 9 cut(s) 7, 329, 1055, 1235, 1357, 1472, 1927, 2117, 2283
AcuI CTGAAG 3 cut(s) 812, 1791, 2574
AdeI CACNNNGTG 2 cut(s) 1142, 2311
AfaI GTAC 7 cut(s) 191, 936, 1167, 1375, 1491, 2073, 2211
AfeI AGCGCT 1 cut(s) 1319
AfiI CCNNNNNNNGG 3 cut(s) 240, 773, 2136
AflIII ACRYGT 2 cut(s) 1072, 1199
AjnI CCWGG 5 cut(s) 238, 314, 1758, 1771, 2307
Alw21I GWGCWC 2 cut(s) 88, 1891
Alw26I GTCTC 2 cut(s) 431, 518
AlwI GGATC 6 cut(s) 544, 564, 653, 666, 1781, 1920
AlwNI CAGNNNCTG 4 cut(s) 1293, 1552, 1772, 1877
Aor51HI AGCGCT 1 cut(s) 1319
AoxI GGCC 7 cut(s) 548, 644, 959, 1570, 1594, 2289, 2427
ApeKI GCWGC 9 cut(s) 148, 1619, 1715, 1766, 2042, 2045, 2201, 2204, 2708
ApoI RAATTY 9 cut(s) 7, 329, 1055, 1235, 1357, 1472, 1927, 2117, 2283
AseI ATTAAT 1 cut(s) 168
AspLEI GCGC 3 cut(s) 1320, 1737, 2400
AspS9I GGNCC 7 cut(s) 508, 644, 959, 1571, 2290, 2340, 2428
AsuC2I CCSGG 1 cut(s) 142
AsuHPI GGTGA 8 cut(s) 583, 740, 996, 1883, 2123, 2297, 2323, 2542
AsuII TTCGAA 2 cut(s) 102, 1323
AvaII GGWCC 2 cut(s) 508, 2340
AxyI CCTNAGG 1 cut(s) 2003
BaeGI GKGCMC 1 cut(s) 2143
BalI TGGCCA 1 cut(s) 1596
BamHI GGATCC 1 cut(s) 658
BanII GRGCYC 1 cut(s) 315
BarI GAAGNNNNNNTAC 2 cut(s) 1393, 1425
Bbv12I GWGCWC 2 cut(s) 88, 1891
BbvCI CCTCAGC 1 cut(s) 1125
BbvI GCAGC 8 cut(s) 160, 1606, 1727, 1778, 2029, 2032, 2188, 2216
BccI CCATC 8 cut(s) 394, 779, 810, 1689, 1970, 1985, 2102, 2580
BceAI ACGGC 1 cut(s) 1716
BciT130I CCWGG 5 cut(s) 240, 316, 1760, 1773, 2309
BciVI GTATCC 2 cut(s) 877, 2559
BclI TGATCA 2 cut(s) 745, 1044
BcnI CCSGG 1 cut(s) 142
BcoDI GTCTC 2 cut(s) 431, 518
BfaI CTAG 7 cut(s) 21, 1379, 1575, 1583, 1829, 1968, 2526
BfmI CTRYAG 4 cut(s) 1821, 2202, 2323, 2703
BfoI RGCGCY 1 cut(s) 1321
BfuAI ACCTGC 1 cut(s) 1828
BfuI GTATCC 2 cut(s) 877, 2559
BisI GCNGC 9 cut(s) 149, 1620, 1716, 1767, 2043, 2046, 2202, 2205, 2709
BlpI GCTNAGC 1 cut(s) 597
BlsI GCNGC 9 cut(s) 150, 1621, 1717, 1768, 2044, 2047, 2203, 2206, 2710
Bme1390I CCNGG 6 cut(s) 142, 240, 316, 1760, 1773, 2309
Bme18I GGWCC 2 cut(s) 508, 2340
BmgT120I GGNCC 7 cut(s) 508, 644, 959, 1571, 2290, 2340, 2428
BmiI GGNNCC 6 cut(s) 645, 660, 874, 960, 1185, 2430
BmrFI CCNGG 6 cut(s) 142, 240, 316, 1760, 1773, 2309
BmrI ACTGGG 3 cut(s) 889, 1021, 1645
BmsI GCATC 7 cut(s) 101, 175, 283, 344, 457, 1437, 2188
BmuI ACTGGG 3 cut(s) 889, 1021, 1645
BplI GAGNNNNNCTC 2 cut(s) 70, 102
BpmI CTGGAG 4 cut(s) 318, 891, 1683, 1794
Bpu10I CCTNAGC 2 cut(s) 87, 1125
Bpu1102I GCTNAGC 1 cut(s) 597
Bpu14I TTCGAA 2 cut(s) 102, 1323
BpuEI CTTGAG 6 cut(s) 39, 97, 249, 617, 1245, 1901
BpuMI CCSGG 1 cut(s) 142
BsaJI CCNNGG 8 cut(s) 239, 314, 639, 1479, 1597, 1758, 1759, 2538
BsaXI ACNNNNNCTCC 2 cut(s) 1656, 1686
Bsc4I CCNNNNNNNGG 3 cut(s) 240, 773, 2136
Bse1I ACTGG 7 cut(s) 370, 551, 864, 884, 918, 1016, 1640
Bse21I CCTNAGG 1 cut(s) 2003
BseBI CCWGG 5 cut(s) 240, 316, 1760, 1773, 2309
BseDI CCNNGG 8 cut(s) 239, 314, 639, 1479, 1597, 1758, 1759, 2538
BseLI CCNNNNNNNGG 3 cut(s) 240, 773, 2136
BseMII CTCAG 8 cut(s) 101, 150, 165, 1116, 1380, 1994, 2415, 2586
BseNI ACTGG 7 cut(s) 370, 551, 864, 884, 918, 1016, 1640
BseSI GKGCMC 1 cut(s) 2143
BseXI GCAGC 8 cut(s) 160, 1606, 1727, 1778, 2029, 2032, 2188, 2216
BseYI CCCAGC 2 cut(s) 955, 2432
BsgI GTGCAG 1 cut(s) 1785
Bsh1236I CGCG 2 cut(s) 68, 2400
BshFI GGCC 7 cut(s) 550, 646, 961, 1572, 1596, 2291, 2429
BsiHKAI GWGCWC 2 cut(s) 88, 1891
BsiSI CCGG 1 cut(s) 142
BslFI GGGAC 2 cut(s) 1454, 2647
BslI CCNNNNNNNGG 3 cut(s) 240, 773, 2136
BsmAI GTCTC 2 cut(s) 431, 518
BsmFI GGGAC 2 cut(s) 1454, 2647
BsmI GAATGC 1 cut(s) 369
BsnI GGCC 7 cut(s) 550, 646, 961, 1572, 1596, 2291, 2429
Bsp119I TTCGAA 2 cut(s) 102, 1323
Bsp1286I GDGCHC 4 cut(s) 88, 315, 1891, 2143
Bsp143I GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
Bsp1720I GCTNAGC 1 cut(s) 597
Bsp19I CCATGG 1 cut(s) 2538
BspACI CCGC 3 cut(s) 198, 594, 2400
BspANI GGCC 7 cut(s) 550, 646, 961, 1572, 1596, 2291, 2429
BspCNI CTCAG 8 cut(s) 100, 151, 166, 1117, 1381, 1995, 2416, 2587
BspFNI CGCG 2 cut(s) 68, 2400
BspHI TCATGA 1 cut(s) 973
BspLI GGNNCC 6 cut(s) 645, 660, 874, 960, 1185, 2430
BspMAI CTGCAG 2 cut(s) 1825, 2206
BspMI ACCTGC 1 cut(s) 1828
BspPI GGATC 6 cut(s) 544, 564, 653, 666, 1781, 1920
BspT104I TTCGAA 2 cut(s) 102, 1323
BsrI ACTGG 7 cut(s) 370, 551, 864, 884, 918, 1016, 1640
BssECI CCNNGG 8 cut(s) 239, 314, 639, 1479, 1597, 1758, 1759, 2538
BssMI GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
BssT1I CCWWGG 4 cut(s) 639, 1479, 1597, 2538
Bst2UI CCWGG 5 cut(s) 240, 316, 1760, 1773, 2309
Bst4CI ACNGT 9 cut(s) 359, 464, 806, 889, 1289, 1400, 1512, 1741, 2580
BstAPI GCANNNNNTGC 2 cut(s) 1877, 1886
BstBI TTCGAA 2 cut(s) 102, 1323
BstC8I GCNNGC 6 cut(s) 136, 566, 1215, 1533, 1554, 2438
BstDSI CCRYGG 1 cut(s) 2538
BstEII GGTNACC 1 cut(s) 2303
BstFNI CGCG 2 cut(s) 68, 2400
BstH2I RGCGCY 1 cut(s) 1321
BstHHI GCGC 3 cut(s) 1320, 1737, 2400
BstKTI GATC 9 cut(s) 325, 539, 559, 661, 748, 1047, 1789, 1915, 2566
BstMAI GTCTC 2 cut(s) 431, 518
BstMBI GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
BstNI CCWGG 5 cut(s) 240, 316, 1760, 1773, 2309
BstNSI RCATGY 4 cut(s) 1076, 1203, 2195, 2440
BstPI GGTNACC 1 cut(s) 2303
BstSCI CCNGG 6 cut(s) 140, 238, 314, 1758, 1771, 2307
BstSFI CTRYAG 4 cut(s) 1821, 2202, 2323, 2703
BstSLI GKGCMC 1 cut(s) 2143
BstUI CGCG 2 cut(s) 68, 2400
BstV1I GCAGC 8 cut(s) 160, 1606, 1727, 1778, 2029, 2032, 2188, 2216
BstX2I RGATCY 3 cut(s) 658, 1786, 1912
BstXI CCANNNNNNTGG 1 cut(s) 1213
BstYI RGATCY 3 cut(s) 658, 1786, 1912
Bsu36I CCTNAGG 1 cut(s) 2003
BsuI GTATCC 2 cut(s) 877, 2559
BsuRI GGCC 7 cut(s) 550, 646, 961, 1572, 1596, 2291, 2429
BtgI CCRYGG 1 cut(s) 2538
BtgZI GCGATG 1 cut(s) 1738
BtsIMutI CAGTG 5 cut(s) 364, 621, 925, 1294, 2592
BveI ACCTGC 1 cut(s) 1828
Cac8I GCNNGC 6 cut(s) 136, 566, 1215, 1533, 1554, 2438
CaiI CAGNNNCTG 4 cut(s) 1293, 1552, 1772, 1877
CciI TCATGA 1 cut(s) 973
CfoI GCGC 3 cut(s) 1320, 1737, 2400
Cfr13I GGNCC 7 cut(s) 508, 644, 959, 1571, 2290, 2340, 2428
CseI GACGC 2 cut(s) 74, 1660
CsiI ACCWGGT 1 cut(s) 2307
Csp6I GTAC 7 cut(s) 190, 935, 1166, 1374, 1490, 2072, 2210
CviQI GTAC 7 cut(s) 190, 935, 1166, 1374, 1490, 2072, 2210
DpnI GATC 9 cut(s) 324, 538, 558, 660, 747, 1046, 1788, 1914, 2565
DpnII GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
DraIII CACNNNGTG 2 cut(s) 1142, 2311
EaeI YGGCCR 1 cut(s) 1594
Eco130I CCWWGG 4 cut(s) 639, 1479, 1597, 2538
Eco24I GRGCYC 1 cut(s) 315
Eco32I GATATC 1 cut(s) 1865
Eco47I GGWCC 2 cut(s) 508, 2340
Eco47III AGCGCT 1 cut(s) 1319
Eco57I CTGAAG 3 cut(s) 812, 1791, 2574
Eco81I CCTNAGG 1 cut(s) 2003
Eco91I GGTNACC 1 cut(s) 2303
EcoO109I RGGNCCY 4 cut(s) 644, 959, 1571, 2428
EcoO65I GGTNACC 1 cut(s) 2303
EcoRI GAATTC 1 cut(s) 7
EcoRII CCWGG 5 cut(s) 238, 314, 1758, 1771, 2307
EcoRV GATATC 1 cut(s) 1865
EcoT14I CCWWGG 4 cut(s) 639, 1479, 1597, 2538
EcoT38I GRGCYC 1 cut(s) 315
ErhI CCWWGG 4 cut(s) 639, 1479, 1597, 2538
FaqI GGGAC 2 cut(s) 1454, 2647
FauI CCCGC 2 cut(s) 205, 2393
FbaI TGATCA 2 cut(s) 745, 1044
Fnu4HI GCNGC 9 cut(s) 149, 1620, 1716, 1767, 2043, 2046, 2202, 2205, 2709
FriOI GRGCYC 1 cut(s) 315
Fsp4HI GCNGC 9 cut(s) 149, 1620, 1716, 1767, 2043, 2046, 2202, 2205, 2709
FspBI CTAG 7 cut(s) 21, 1379, 1575, 1583, 1829, 1968, 2526
GlaI GCGC 3 cut(s) 1319, 1736, 2399
GluI GCNGC 9 cut(s) 149, 1620, 1716, 1767, 2043, 2046, 2202, 2205, 2709
GsaI CCCAGC 2 cut(s) 959, 2436
GsuI CTGGAG 4 cut(s) 318, 891, 1683, 1794
HaeII RGCGCY 1 cut(s) 1321
HaeIII GGCC 7 cut(s) 550, 646, 961, 1572, 1596, 2291, 2429
HapII CCGG 1 cut(s) 142
HgaI GACGC 2 cut(s) 74, 1660
HhaI GCGC 3 cut(s) 1320, 1737, 2400
Hin6I GCGC 3 cut(s) 1318, 1735, 2398
HinP1I GCGC 3 cut(s) 1318, 1735, 2398
HincII GTYRAC 2 cut(s) 1070, 1986
HindII GTYRAC 2 cut(s) 1070, 1986
HindIII AAGCTT 2 cut(s) 73, 421
HinfI GANTC 9 cut(s) 113, 777, 914, 1248, 1385, 1874, 1898, 2009, 2599
HpaI GTTAAC 1 cut(s) 1986
HpaII CCGG 1 cut(s) 142
HphI GGTGA 8 cut(s) 583, 740, 996, 1883, 2123, 2297, 2323, 2542
Hpy166II GTNNAC 4 cut(s) 1070, 1168, 1376, 1986
Hpy8I GTNNAC 4 cut(s) 1070, 1168, 1376, 1986
HpyCH4III ACNGT 9 cut(s) 359, 464, 806, 889, 1289, 1400, 1512, 1741, 2580
HpyCH4IV ACGT 1 cut(s) 2182
HpySE526I ACGT 1 cut(s) 2182
HspAI GCGC 3 cut(s) 1318, 1735, 2398
Ksp22I TGATCA 2 cut(s) 745, 1044
KspAI GTTAAC 1 cut(s) 1986
Kzo9I GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
LmnI GCTCC 7 cut(s) 143, 611, 872, 1495, 1616, 1774, 1775
Lsp1109I GCAGC 8 cut(s) 160, 1606, 1727, 1778, 2029, 2032, 2188, 2216
LweI GCATC 7 cut(s) 101, 175, 283, 344, 457, 1437, 2188
MabI ACCWGGT 1 cut(s) 2307
MaeI CTAG 7 cut(s) 21, 1379, 1575, 1583, 1829, 1968, 2526
MaeII ACGT 1 cut(s) 2182
MalI GATC 9 cut(s) 324, 538, 558, 660, 747, 1046, 1788, 1914, 2565
MboI GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
MboII GAAGA 9 cut(s) 9, 116, 175, 443, 805, 848, 1139, 1344, 2391
MflI RGATCY 3 cut(s) 658, 1786, 1912
MhlI GDGCHC 4 cut(s) 88, 315, 1891, 2143
MlsI TGGCCA 1 cut(s) 1596
MluNI TGGCCA 1 cut(s) 1596
MlyI GAGTC 2 cut(s) 923, 1257
MmeI TCCRAC 5 cut(s) 535, 1814, 2216, 2290, 2354
Mox20I TGGCCA 1 cut(s) 1596
MscI TGGCCA 1 cut(s) 1596
MseI TTAA 9 cut(s) 168, 762, 1340, 1518, 1781, 1905, 1985, 2015, 2582
MslI CAYNNNNRTG 2 cut(s) 2196, 2696
Msp20I TGGCCA 1 cut(s) 1596
MspI CCGG 1 cut(s) 142
MspR9I CCNGG 6 cut(s) 142, 240, 316, 1760, 1773, 2309
Mva1269I GAATGC 1 cut(s) 369
MvaI CCWGG 5 cut(s) 240, 316, 1760, 1773, 2309
MvnI CGCG 2 cut(s) 68, 2400
NciI CCSGG 1 cut(s) 142
NcoI CCATGG 1 cut(s) 2538
NdeII GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
NlaIV GGNNCC 6 cut(s) 645, 660, 874, 960, 1185, 2430
NmuCI GTSAC 5 cut(s) 589, 1289, 1424, 2303, 2590
NspI RCATGY 4 cut(s) 1076, 1203, 2195, 2440
NspV TTCGAA 2 cut(s) 102, 1323
PaeI GCATGC 1 cut(s) 2440
PagI TCATGA 1 cut(s) 973
PaqCI CACCTGC 1 cut(s) 1828
PasI CCCWGGG 1 cut(s) 1759
PciI ACATGT 2 cut(s) 1072, 1199
PctI GAATGC 1 cut(s) 369
PfeI GAWTC 7 cut(s) 113, 777, 1385, 1874, 1898, 2009, 2599
PflFI GACNNNGTC 1 cut(s) 1637
PfoI TCCNGGA 1 cut(s) 1771
PkrI GCNGC 9 cut(s) 150, 1621, 1717, 1768, 2044, 2047, 2203, 2206, 2710
PleI GAGTC 2 cut(s) 922, 1256
PpsI GAGTC 2 cut(s) 922, 1256
PscI ACATGT 2 cut(s) 1072, 1199
PshBI ATTAAT 1 cut(s) 168
PsiI TTATAA 1 cut(s) 126
Psp6I CCWGG 5 cut(s) 238, 314, 1758, 1771, 2307
PspEI GGTNACC 1 cut(s) 2303
PspFI CCCAGC 2 cut(s) 955, 2432
PspGI CCWGG 5 cut(s) 238, 314, 1758, 1771, 2307
PspN4I GGNNCC 6 cut(s) 645, 660, 874, 960, 1185, 2430
PspPI GGNCC 7 cut(s) 508, 644, 959, 1571, 2290, 2340, 2428
PstI CTGCAG 2 cut(s) 1825, 2206
PstNI CAGNNNCTG 4 cut(s) 1293, 1552, 1772, 1877
PsuI RGATCY 3 cut(s) 658, 1786, 1912
PsyI GACNNNGTC 1 cut(s) 1637
RsaI GTAC 7 cut(s) 191, 936, 1167, 1375, 1491, 2073, 2211
RsaNI GTAC 7 cut(s) 190, 935, 1166, 1374, 1490, 2072, 2210
RseI CAYNNNNRTG 2 cut(s) 2196, 2696
SaqAI TTAA 9 cut(s) 168, 762, 1340, 1518, 1781, 1905, 1985, 2015, 2582
SatI GCNGC 9 cut(s) 149, 1620, 1716, 1767, 2043, 2046, 2202, 2205, 2709
Sau3AI GATC 9 cut(s) 322, 536, 556, 658, 745, 1044, 1786, 1912, 2563
Sau96I GGNCC 7 cut(s) 508, 644, 959, 1571, 2290, 2340, 2428
SchI GAGTC 2 cut(s) 923, 1257
ScrFI CCNGG 6 cut(s) 142, 240, 316, 1760, 1773, 2309
SduI GDGCHC 4 cut(s) 88, 315, 1891, 2143
SexAI ACCWGGT 1 cut(s) 2307
SfaNI GCATC 7 cut(s) 101, 175, 283, 344, 457, 1437, 2188
SfcI CTRYAG 4 cut(s) 1821, 2202, 2323, 2703
SfuI TTCGAA 2 cut(s) 102, 1323
SinI GGWCC 2 cut(s) 508, 2340
SmiMI CAYNNNNRTG 2 cut(s) 2196, 2696
SmlI CTYRAG 6 cut(s) 54, 76, 264, 632, 1260, 1916
SmoI CTYRAG 6 cut(s) 54, 76, 264, 632, 1260, 1916
SphI GCATGC 1 cut(s) 2440
SsiI CCGC 3 cut(s) 198, 594, 2400
SspMI CTAG 7 cut(s) 21, 1379, 1575, 1583, 1829, 1968, 2526
StyD4I CCNGG 6 cut(s) 140, 238, 314, 1758, 1771, 2307
StyI CCWWGG 4 cut(s) 639, 1479, 1597, 2538
TaaI ACNGT 9 cut(s) 359, 464, 806, 889, 1289, 1400, 1512, 1741, 2580
TaiI ACGT 1 cut(s) 2185
TaqI TCGA 9 cut(s) 102, 603, 780, 801, 1323, 1467, 1931, 2575, 2682
TatI WGTACW 2 cut(s) 1373, 1489
TfiI GAWTC 7 cut(s) 113, 777, 1385, 1874, 1898, 2009, 2599
Tru1I TTAA 9 cut(s) 168, 762, 1340, 1518, 1781, 1905, 1985, 2015, 2582
Tru9I TTAA 9 cut(s) 168, 762, 1340, 1518, 1781, 1905, 1985, 2015, 2582
TscAI CASTG 5 cut(s) 364, 628, 925, 1294, 2599
TseFI GTSAC 5 cut(s) 589, 1289, 1424, 2303, 2590
TseI GCWGC 9 cut(s) 148, 1619, 1715, 1766, 2042, 2045, 2201, 2204, 2708
Tsp45I GTSAC 5 cut(s) 589, 1289, 1424, 2303, 2590
TspGWI ACGGA 5 cut(s) 77, 1938, 2003, 2219, 2332
TspRI CASTG 5 cut(s) 364, 628, 925, 1294, 2599
Tth111I GACNNNGTC 1 cut(s) 1637
VpaK11BI GGWCC 2 cut(s) 508, 2340
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 9 cut(s) 7, 329, 1055, 1235, 1357, 1472, 1927, 2117, 2283
XbaI TCTAGA 1 cut(s) 1582
XceI RCATGY 4 cut(s) 1076, 1203, 2195, 2440
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XspI CTAG 7 cut(s) 21, 1379, 1575, 1583, 1829, 1968, 2526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.