pycom01g05850

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0001949
Physical Location & Seq
Forward (+)
5763873 .. 5774875
11003 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g05850.11

Sequence Viewer

Length: 3327 bp
ATGGCGGAGATCAAGCGCTACGGTCTTGGTAATCAATTGGATATTGCGCAGATACTCTTAGAAGCTAAACATCGCTGGCTACGCCCGGCTGAGATATGTGAAATTCTTCGAAATTTCCAAAAATTTGACATTTCTTCAGAGCCTGCAAATATGCCACCCGGTGGATCGATCTTTCTATTTGATCGGAAGGTGCTTAGATATTTCAGGAAAGATGGCCATAACTGGAGGAAGAAAAAAGATGGAAAGACAGTGAGGGAAGCCCATGAGAGACTTAAGGCTGGAAGTGTTGATGTATTGCAGTGCTATTATGCACATGGAGAAGAAAATGAAAATTTCCAAAGACGCAGTTATTGGACGCTTGAGGAGGATCTGCAACACATAGTTCTTGTCCACTACCTGGAAGTTAAGGGAAATAGGACAAACTATAACCATTTTAAAGGCACTGAAGAAGCTGTTCCTTATTCCCATGAAACTGAAGAAATCGCACTCAATTCTGAGATGGACAATTCTGTTTCTTCTAGTTTCAATCCAAATAATTTCCAAATGTGTTCCCAAGCTGCTGATACAACAAGCTTGAGTAGTGCTCAAGCATCAGAGTTCGAAGATGCTGAATCAGCATATAAACACCAAGCAAGTTCCCAGTTACAACCTTTTCTCGAATTACTACAGCCTAAGGCTGAGAAGATCAATGCTGGATTCTCTAATGCATTCTATCCGATGTCATTTTCAAATGAATATCAAGAAAAATTGTCGGCCGTTCCTGGGGTGAATTTTAGCTCACGTACTGAAGCATATAGGAAGGAAGATGGTAATGATGCTGGCGTTACCTATGATCCCAGGAAAAATCTTAACTCCGCAGTATGGGATGGTGCCTTGGGGAATATTACTACTGGCTTTCAATCTTTGCCTTTTCAGCCATCAATTTCAACAACACATTCTGATTCAATAGGAATTATCTCTCAGCAAGAGAATGAGACGCTTGGGCACCTTTTCACAGACAACTTTGGTAAAAAGCAGATCTACGAGGATAAACCAAGGGTCCAACAAAGCTGGCAGACTTTGGAAGCCAACTCTTCTGGTTCATCCAGCTGGCCTGTGGATCAGAACTTGCATTCAGACACAGCATATGATGTCACCACAAGGTTCCATGGAGGAGTCGATGATTCTAATTTACTCAATTCTCCAGTGTGCCGTGTGGATTCTGAGGAGACAAATAATTATTCTATGCCAAATGACCTTCAAATACAGCCTTCAAACACTGAGCAAGAATATCATCTGAAGTCTATTTCAAAGAGAAATGAAACCATAGAGGGAAAATACAATCACACTTTTGCTACAAAGCCTTTGTTAGATGAAGGCTTGAAGAAGCTTGACAGTTTCAGCCGATGGATGAGCAAAGAACTTGGAGACGTGGAAGAGACACACACACAATCTAATTCAGAGACGTACTGGGATACTGTTGAAAGTGACAGTGGGGTTGATGAGTCCAGGATTCCTCTTCAAGTACGCTTGGATAGCTATATGCTGGGGCCTTCTCTTTCCCAGGACCAGCTCTTTAGCATTATTGATTTCTCACCAAACTGGGCATATGAAAACTCTGAAATCAAGGTTCTGATCGCAGGAAGATTTTTGAAGAGTCAACAAGCAGAAAGTTGTAAGTGGTCATGCATGTTTGGGGAAGTTGAAGTTCCTGCTGAGGTTATAGCAGATGGTGTTCTTCGTTGTTATACTCCCATCCATAAGGCTGGGAGAATTCCTTTCTACGTCACATGTTCCAATAGGCTGGCTTGTAGTGAAATCAGGGAATTTGAATACCGAGTCGGGCAAATACCAGATTATGATGTTAAAGATGATTACAGTGGCTGCACAAATGAAATATTAAATATGCGCTTTGGAAAATTACTGTCTTTAAGCTCTAGCTCTCCAACTTTTGATCCCACCAGCGTAGCAGAGAATTCTGAGATAATCAGTAAAATTGATTTGTTGCTGAAAAATGACAATGGTGAGTGGGACAAGATGCTACAACTTACGTCAGATGAAGACTTTTCCTTGGAGAGAGTAGAGGACCAGTTGCTTCAACAGCTACTTAAAGAGGAGTTGCATGCATGGCTCTTGCAAAAGCTGGCTGCTGGTGGAAAAGGCCCTAGTGTACTAGACGAGGGTGGCCAAGGTGTATTACATTTTGGAGCTGCTCTTGGCTATGATTGGGTCTTACTGCCCACAATAACTGCCGGAGTCAGTGTCAATTTTCGTGACATTAATGGATGGACAGCTCTTCACTGGGCAGCTTTTTGTGGCAGAGAGCGCACAGTTGCTTCCCTCATCTCTCTTGGTGCAGCTCCCGGATTATTAACAGATCCAAGAACCAAATATCCTAATGGAAGAACACCTGCAGACCTAGCTTCTGCACAGGGCCACAAGGGAATTGCTGGTTATCTTGCAGAATCTGCTTTGAGTGACCAACTTTCATTTCTTAATTTGGATATCAAGGAAGACAATAATGCTGATATTTCAGGGGCCAATGCAGTACGAACAGTTTCAGAACAAATTGCAACTCCTATCGGGAATGGGGATTTAACGGATGGGCTTTCCCTGAGGGATTCTTTAACTGCTGTCTGTAATGCTACCCAAGCTGCTGCTCGTATTCATCAAGTGTTCAGGGTAAAATCTTTCCAGCTGAAGCAGTTGAAAGAATATGGTAGTGATAACTTTGGAATTTCAGATGAAGATGCTCTATCAATGATTGCAGTCAAGTCACATAAGCCAAGAAAACGTGCTGAGCATGTGGATGCTGCTGCAATTCGAATACAAAATAAGTTCCGTAGTTGGAAGGGTAGAAAAGATTATTTGATAATCCGACAACGTATTGTCAAAATTCAGGCTCATGTAAGAGGCCACCAGGTGAGGAAAAACTACAGAAAGATAGTCTGGTCCGTAGGAATTGTAGAAAAGATAATTTTGCGTTGGAGAAGAAAAGGAAGTGGTTTGCGTGGGTTCAAGCCAGAACCGCTTACTGAAGCCCCCAGCATGCAAGCTTCATCATCGAAGGATGATGATTATGATGTACTGAAAGAAGGAAGGAAGCAAACAGAGCAAAGGTTGCAAAAGGCGCTTGCCAGGGTGAAGTCCATGGTTCAATATCCTGAAGCAAGAGATCAATACTGTCGGCTGCTGAATGTTGTTACTGAGATACAGGAAACCAAGGTGGTATATGATAGTGGTACGACTAGTTCAGAAGGGAGAGTCGATATGGATCACGACCTCATAGATATTGCAGCATTGTTGGACGAAGAAGACATTTACATGCCTACCGAAGCTCCAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

1109

Amino Acids

124.7

Weight (kDa)

5.38

Isoelectric Point (pI)

42.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2398
Acc16I TGCGCA 1 cut(s) 48
Acc36I ACCTGC 1 cut(s) 2398
AccB1I GGYRCC 2 cut(s) 869, 984
AccB7I CCANNNNNTGG 2 cut(s) 161, 397
AciI CCGC 3 cut(s) 5, 855, 3008
AclWI GGATC 7 cut(s) 172, 375, 827, 1107, 1928, 2351, 3261
AcoI YGGCCR 3 cut(s) 214, 753, 2164
AcuI CTGAAG 8 cut(s) 120, 465, 495, 807, 1298, 2699, 3036, 3165
AdeI CACNNNGTG 2 cut(s) 161, 2902
AfaI GTAC 7 cut(s) 784, 1448, 1506, 2151, 2529, 3066, 3223
AfeI AGCGCT 1 cut(s) 17
AfiI CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
AflII CTTAAG 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 1769
AhlI ACTAGT 1 cut(s) 3227
AjiI CACGTC 1 cut(s) 1411
AjnI CCWGG 7 cut(s) 396, 760, 836, 1487, 1542, 2898, 3116
AjuI GAANNNNNNNTTGG 2 cut(s) 2454, 2486
AloI GAACNNNNNNTCC 2 cut(s) 2356, 2388
Alw21I GWGCWC 1 cut(s) 586
Alw26I GTCTC 6 cut(s) 262, 968, 1202, 1401, 1412, 1436
AlwI GGATC 7 cut(s) 172, 375, 827, 1107, 1928, 2351, 3261
AlwNI CAGNNNCTG 3 cut(s) 143, 1863, 2447
Aor51HI AGCGCT 1 cut(s) 17
AoxI GGCC 9 cut(s) 214, 753, 1091, 1529, 2140, 2164, 2413, 2517, 2893
AseI ATTAAT 1 cut(s) 2259
Asp700I GAANNNNTTC 3 cut(s) 105, 453, 2536
AspLEI GCGC 5 cut(s) 18, 49, 1890, 2307, 3112
AspS9I GGNCC 8 cut(s) 1039, 1529, 1546, 2065, 2141, 2413, 2517, 2931
AsuC2I CCSGG 3 cut(s) 86, 159, 2343
AsuHPI GGTGA 6 cut(s) 778, 1126, 1566, 2015, 2914, 3133
AsuII TTCGAA 3 cut(s) 109, 600, 2803
AvaII GGWCC 4 cut(s) 1039, 1546, 2065, 2931
AxyI CCTNAGG 2 cut(s) 672, 2594
BaeGI GKGCMC 1 cut(s) 987
BalI TGGCCA 2 cut(s) 216, 2166
BanI GGYRCC 2 cut(s) 869, 984
BbsI GAAGAC 3 cut(s) 2046, 2499, 3300
Bbv12I GWGCWC 1 cut(s) 586
BbvCI CCTCAGC 1 cut(s) 1695
BceAI ACGGC 2 cut(s) 740, 1176
BciT130I CCWGG 7 cut(s) 398, 762, 838, 1489, 1544, 2900, 3118
BciVI GTATCC 1 cut(s) 1447
BcnI CCSGG 3 cut(s) 86, 159, 2343
BcoDI GTCTC 6 cut(s) 262, 968, 1202, 1401, 1412, 1436
BcuI ACTAGT 1 cut(s) 3227
BfaI CTAG 6 cut(s) 519, 1917, 2145, 2153, 2399, 3228
BfmI CTRYAG 3 cut(s) 665, 2391, 2914
BfoI RGCGCY 2 cut(s) 19, 3113
BfrI CTTAAG 1 cut(s) 272
BfuAI ACCTGC 1 cut(s) 2398
BfuI GTATCC 1 cut(s) 1447
BglII AGATCT 1 cut(s) 1017
BlpI GCTNAGC 1 cut(s) 2778
Bme18I GGWCC 4 cut(s) 1039, 1546, 2065, 2931
BmgBI CACGTC 1 cut(s) 1411
BmgT120I GGNCC 8 cut(s) 1039, 1529, 1546, 2065, 2141, 2413, 2517, 2931
BmiI GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1530, 2518
BmrI ACTGGG 4 cut(s) 634, 1459, 1591, 2290
BmsI GCATC 6 cut(s) 595, 599, 805, 2007, 2719, 2779
BmuI ACTGGG 4 cut(s) 634, 1459, 1591, 2290
BpiI GAAGAC 3 cut(s) 2046, 2499, 3300
BplI GAGNNNNNCTC 2 cut(s) 568, 600
BpmI CTGGAG 3 cut(s) 244, 1167, 3303
Bpu10I CCTNAGC 1 cut(s) 1695
Bpu1102I GCTNAGC 1 cut(s) 2778
Bpu14I TTCGAA 3 cut(s) 109, 600, 2803
BpuEI CTTGAG 3 cut(s) 380, 570, 595
BpuMI CCSGG 3 cut(s) 86, 159, 2343
Bsa29I ATCGAT 1 cut(s) 167
BsaAI YACGTR 1 cut(s) 782
BsaBI GATNNNNATC 1 cut(s) 3252
BsaXI ACNNNNNCTCC 3 cut(s) 2226, 2256, 3301
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
Bse1I ACTGG 8 cut(s) 227, 640, 895, 1184, 1454, 1586, 2068, 2285
Bse21I CCTNAGG 2 cut(s) 672, 2594
Bse8I GATNNNNATC 1 cut(s) 3252
BseBI CCWGG 7 cut(s) 398, 762, 838, 1489, 1544, 2900, 3118
BseCI ATCGAT 1 cut(s) 167
BseGI GGATG 8 cut(s) 871, 1082, 1395, 1734, 2270, 2587, 2794, 3055
BseJI GATNNNNATC 1 cut(s) 3252
BseLI CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
BseNI ACTGG 8 cut(s) 227, 640, 895, 1184, 1454, 1586, 2068, 2285
BseRI GAGGAG 4 cut(s) 377, 1167, 1220, 2108
BseSI GKGCMC 1 cut(s) 987
BseX3I CGGCCG 1 cut(s) 753
BseYI CCCAGC 3 cut(s) 1525, 1745, 3023
BsgI GTGCAG 3 cut(s) 1849, 2355, 2391
Bsh1285I CGRYCG 1 cut(s) 756
BshFI GGCC 9 cut(s) 216, 755, 1093, 1531, 2142, 2166, 2415, 2519, 2895
BshNI GGYRCC 2 cut(s) 869, 984
BshVI ATCGAT 1 cut(s) 167
BsiEI CGRYCG 1 cut(s) 756
BsiHKAI GWGCWC 1 cut(s) 586
BsiSI CCGG 4 cut(s) 86, 159, 2232, 2343
BslFI GGGAC 1 cut(s) 2024
BslI CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
BsmAI GTCTC 6 cut(s) 262, 968, 1202, 1401, 1412, 1436
BsmBI CGTCTC 3 cut(s) 968, 1401, 1436
BsmFI GGGAC 1 cut(s) 2024
BsmI GAATGC 2 cut(s) 707, 1111
BsnI GGCC 9 cut(s) 216, 755, 1093, 1531, 2142, 2166, 2415, 2519, 2895
Bsp119I TTCGAA 3 cut(s) 109, 600, 2803
Bsp1286I GDGCHC 2 cut(s) 586, 987
Bsp1720I GCTNAGC 1 cut(s) 2778
Bsp19I CCATGG 2 cut(s) 1147, 3129
BspACI CCGC 3 cut(s) 5, 855, 3008
BspANI GGCC 9 cut(s) 216, 755, 1093, 1531, 2142, 2166, 2415, 2519, 2895
BspDI ATCGAT 1 cut(s) 167
BspLI GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1530, 2518
BspMAI CTGCAG 1 cut(s) 2395
BspMI ACCTGC 1 cut(s) 2398
BspPI GGATC 7 cut(s) 172, 375, 827, 1107, 1928, 2351, 3261
BspQI GCTCTTC 1 cut(s) 2280
BspT104I TTCGAA 3 cut(s) 109, 600, 2803
BspT107I GGYRCC 2 cut(s) 869, 984
BspTI CTTAAG 1 cut(s) 272
BsrI ACTGG 8 cut(s) 227, 640, 895, 1184, 1454, 1586, 2068, 2285
BssT1I CCWWGG 7 cut(s) 873, 1034, 1147, 2049, 2167, 3129, 3201
Bst2UI CCWGG 7 cut(s) 398, 762, 838, 1489, 1544, 2900, 3118
Bst6I CTCTTC 5 cut(s) 1078, 1410, 1503, 1628, 2280
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 2 cut(s) 2447, 3100
BstBAI YACGTR 1 cut(s) 782
BstBI TTCGAA 3 cut(s) 109, 600, 2803
BstDSI CCRYGG 2 cut(s) 1147, 3129
BstF5I GGATG 8 cut(s) 871, 1082, 1395, 1734, 2270, 2587, 2794, 3055
BstH2I RGCGCY 2 cut(s) 19, 3113
BstHHI GCGC 5 cut(s) 18, 49, 1890, 2307, 3112
BstMAI GTCTC 6 cut(s) 262, 968, 1202, 1401, 1412, 1436
BstMCI CGRYCG 1 cut(s) 756
BstNI CCWGG 7 cut(s) 398, 762, 838, 1489, 1544, 2900, 3118
BstNSI RCATGY 6 cut(s) 1672, 1773, 2105, 2786, 3031, 3307
BstSFI CTRYAG 3 cut(s) 665, 2391, 2914
BstSLI GKGCMC 1 cut(s) 987
BstV2I GAAGAC 3 cut(s) 2046, 2499, 3300
BstX2I RGATCY 3 cut(s) 367, 1017, 2356
BstXI CCANNNNNNTGG 2 cut(s) 1745, 1783
BstYI RGATCY 3 cut(s) 367, 1017, 2356
BstZI CGGCCG 1 cut(s) 753
Bsu15I ATCGAT 1 cut(s) 167
Bsu36I CCTNAGG 2 cut(s) 672, 2594
BsuI GTATCC 1 cut(s) 1447
BsuRI GGCC 9 cut(s) 216, 755, 1093, 1531, 2142, 2166, 2415, 2519, 2895
BsuTUI ATCGAT 1 cut(s) 167
BtgI CCRYGG 2 cut(s) 1147, 3129
BtgZI GCGATG 1 cut(s) 56
BtrI CACGTC 1 cut(s) 1411
BtsCI GGATG 8 cut(s) 871, 1082, 1395, 1734, 2270, 2587, 2794, 3055
BtsI GCAGTG 1 cut(s) 305
BtsIMutI CAGTG 9 cut(s) 255, 305, 441, 1191, 1257, 1477, 1864, 2245, 2278
BveI ACCTGC 1 cut(s) 2398
CaiI CAGNNNCTG 3 cut(s) 143, 1863, 2447
CfoI GCGC 5 cut(s) 18, 49, 1890, 2307, 3112
Cfr13I GGNCC 8 cut(s) 1039, 1529, 1546, 2065, 2141, 2413, 2517, 2931
ClaI ATCGAT 1 cut(s) 167
CseI GACGC 3 cut(s) 351, 364, 985
CsiI ACCWGGT 1 cut(s) 2898
Csp6I GTAC 7 cut(s) 783, 1447, 1505, 2150, 2528, 3065, 3222
CviQI GTAC 7 cut(s) 783, 1447, 1505, 2150, 2528, 3065, 3222
DraI TTTAAA 1 cut(s) 436
DraIII CACNNNGTG 2 cut(s) 161, 2902
EaeI YGGCCR 3 cut(s) 214, 753, 2164
EagI CGGCCG 1 cut(s) 753
Eam1104I CTCTTC 5 cut(s) 1078, 1410, 1503, 1628, 2280
EarI CTCTTC 5 cut(s) 1078, 1410, 1503, 1628, 2280
EciI GGCGGA 1 cut(s) 20
EclXI CGGCCG 1 cut(s) 753
Eco130I CCWWGG 7 cut(s) 873, 1034, 1147, 2049, 2167, 3129, 3201
Eco32I GATATC 1 cut(s) 2485
Eco47I GGWCC 4 cut(s) 1039, 1546, 2065, 2931
Eco47III AGCGCT 1 cut(s) 17
Eco52I CGGCCG 1 cut(s) 753
Eco57I CTGAAG 8 cut(s) 120, 465, 495, 807, 1298, 2699, 3036, 3165
Eco81I CCTNAGG 2 cut(s) 672, 2594
EcoO109I RGGNCCY 2 cut(s) 1529, 2141
EcoRI GAATTC 2 cut(s) 1752, 1954
EcoRII CCWGG 7 cut(s) 396, 760, 836, 1487, 1542, 2898, 3116
EcoRV GATATC 1 cut(s) 2485
EcoT14I CCWWGG 7 cut(s) 873, 1034, 1147, 2049, 2167, 3129, 3201
EcoT22I ATGCAT 3 cut(s) 709, 1670, 2107
ErhI CCWWGG 7 cut(s) 873, 1034, 1147, 2049, 2167, 3129, 3201
Esp3I CGTCTC 3 cut(s) 968, 1401, 1436
FaqI GGGAC 1 cut(s) 2024
FauNDI CATATG 2 cut(s) 1126, 1588
FokI GGATG 8 cut(s) 878, 1069, 1402, 1721, 2277, 2594, 2801, 3062
FspBI CTAG 6 cut(s) 519, 1917, 2145, 2153, 2399, 3228
FspI TGCGCA 1 cut(s) 48
GlaI GCGC 5 cut(s) 17, 48, 1889, 2306, 3111
GsaI CCCAGC 3 cut(s) 1529, 1749, 3027
GsuI CTGGAG 3 cut(s) 244, 1167, 3303
HaeII RGCGCY 2 cut(s) 19, 3113
HaeIII GGCC 9 cut(s) 216, 755, 1093, 1531, 2142, 2166, 2415, 2519, 2895
HapII CCGG 4 cut(s) 86, 159, 2232, 2343
HgaI GACGC 3 cut(s) 351, 364, 985
HhaI GCGC 5 cut(s) 18, 49, 1890, 2307, 3112
Hin6I GCGC 5 cut(s) 16, 47, 1888, 2305, 3110
HinP1I GCGC 5 cut(s) 16, 47, 1888, 2305, 3110
HincII GTYRAC 1 cut(s) 1640
HindII GTYRAC 1 cut(s) 1640
HindIII AAGCTT 3 cut(s) 571, 1367, 3033
HpaII CCGG 4 cut(s) 86, 159, 2232, 2343
HphI GGTGA 6 cut(s) 778, 1126, 1566, 2015, 2914, 3133
Hpy166II GTNNAC 3 cut(s) 391, 1640, 2150
Hpy188III TCNNGA 8 cut(s) 205, 656, 740, 2252, 2563, 3143, 3257, 3320
Hpy8I GTNNAC 3 cut(s) 391, 1640, 2150
HpyCH4IV ACGT 7 cut(s) 781, 1410, 1445, 1764, 2030, 2773, 2863
HpySE526I ACGT 7 cut(s) 781, 1410, 1445, 1764, 2030, 2773, 2863
HspAI GCGC 5 cut(s) 16, 47, 1888, 2305, 3110
LguI GCTCTTC 1 cut(s) 2280
LmnI GCTCC 3 cut(s) 2186, 2344, 3322
LweI GCATC 6 cut(s) 595, 599, 805, 2007, 2719, 2779
MabI ACCWGGT 1 cut(s) 2898
MaeI CTAG 6 cut(s) 519, 1917, 2145, 2153, 2399, 3228
MaeII ACGT 7 cut(s) 781, 1410, 1445, 1764, 2030, 2773, 2863
MaeIII GTNAC 9 cut(s) 642, 823, 1132, 1466, 1765, 2252, 2456, 2754, 3181
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 3 cut(s) 367, 1017, 2356
MhlI GDGCHC 2 cut(s) 586, 987
MlsI TGGCCA 2 cut(s) 216, 2166
MluNI TGGCCA 2 cut(s) 216, 2166
MlyI GAGTC 6 cut(s) 1164, 1493, 1645, 1827, 2244, 3252
MmeI TCCRAC 6 cut(s) 1066, 1949, 2807, 2881, 2945, 3264
Mox20I TGGCCA 2 cut(s) 216, 2166
Mph1103I ATGCAT 3 cut(s) 709, 1670, 2107
MroXI GAANNNNTTC 3 cut(s) 105, 453, 2536
MscI TGGCCA 2 cut(s) 216, 2166
MslI CAYNNNNRTG 2 cut(s) 2787, 3302
Msp20I TGGCCA 2 cut(s) 216, 2166
MspA1I CMGCKG 2 cut(s) 1089, 2677
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 4 cut(s) 86, 159, 2232, 2343
MunI CAATTG 1 cut(s) 35
Mva1269I GAATGC 2 cut(s) 707, 1111
MvaI CCWGG 7 cut(s) 398, 762, 838, 1489, 1544, 2900, 3118
NciI CCSGG 3 cut(s) 86, 159, 2343
NcoI CCATGG 2 cut(s) 1147, 3129
NdeI CATATG 2 cut(s) 1126, 1588
NlaIV GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1530, 2518
NmuCI GTSAC 6 cut(s) 1132, 1466, 1765, 2252, 2456, 2754
NsbI TGCGCA 1 cut(s) 48
NsiI ATGCAT 3 cut(s) 709, 1670, 2107
NspI RCATGY 6 cut(s) 1672, 1773, 2105, 2786, 3031, 3307
NspV TTCGAA 3 cut(s) 109, 600, 2803
PaeI GCATGC 2 cut(s) 2105, 3031
PaqCI CACCTGC 1 cut(s) 2398
PciI ACATGT 1 cut(s) 1769
PciSI GCTCTTC 1 cut(s) 2280
PctI GAATGC 2 cut(s) 707, 1111
PdmI GAANNNNTTC 3 cut(s) 105, 453, 2536
PfeI GAWTC 8 cut(s) 611, 696, 941, 1163, 1199, 1492, 2444, 2600
PflMI CCANNNNNTGG 2 cut(s) 161, 397
PfoI TCCNGGA 2 cut(s) 1487, 2341
PleI GAGTC 6 cut(s) 1163, 1492, 1644, 1826, 2243, 3251
PpsI GAGTC 6 cut(s) 1163, 1492, 1644, 1826, 2243, 3251
Ppu21I YACGTR 1 cut(s) 782
PscI ACATGT 1 cut(s) 1769
PshBI ATTAAT 1 cut(s) 2259
Psp6I CCWGG 7 cut(s) 396, 760, 836, 1487, 1542, 2898, 3116
PspFI CCCAGC 3 cut(s) 1525, 1745, 3023
PspGI CCWGG 7 cut(s) 396, 760, 836, 1487, 1542, 2898, 3116
PspN4I GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1530, 2518
PspPI GGNCC 8 cut(s) 1039, 1529, 1546, 2065, 2141, 2413, 2517, 2931
PsrI GAACNNNNNNTAC 2 cut(s) 3214, 3246
PstI CTGCAG 1 cut(s) 2395
PstNI CAGNNNCTG 3 cut(s) 143, 1863, 2447
PsuI RGATCY 3 cut(s) 367, 1017, 2356
PvuII CAGCTG 2 cut(s) 1089, 2677
RsaI GTAC 7 cut(s) 784, 1448, 1506, 2151, 2529, 3066, 3223
RsaNI GTAC 7 cut(s) 783, 1447, 1505, 2150, 2528, 3065, 3222
RseI CAYNNNNRTG 2 cut(s) 2787, 3302
SapI GCTCTTC 1 cut(s) 2280
Sau96I GGNCC 8 cut(s) 1039, 1529, 1546, 2065, 2141, 2413, 2517, 2931
SchI GAGTC 6 cut(s) 1164, 1493, 1645, 1827, 2244, 3252
SduI GDGCHC 2 cut(s) 586, 987
SexAI ACCWGGT 1 cut(s) 2898
SfaNI GCATC 6 cut(s) 595, 599, 805, 2007, 2719, 2779
SfcI CTRYAG 3 cut(s) 665, 2391, 2914
SfuI TTCGAA 3 cut(s) 109, 600, 2803
SinI GGWCC 4 cut(s) 1039, 1546, 2065, 2931
SmiMI CAYNNNNRTG 2 cut(s) 2787, 3302
SmlI CTYRAG 4 cut(s) 272, 359, 574, 585
SmoI CTYRAG 4 cut(s) 272, 359, 574, 585
SpeI ACTAGT 1 cut(s) 3227
SphI GCATGC 2 cut(s) 2105, 3031
SsiI CCGC 3 cut(s) 5, 855, 3008
SspI AATATT 2 cut(s) 883, 1878
SspMI CTAG 6 cut(s) 519, 1917, 2145, 2153, 2399, 3228
StyI CCWWGG 7 cut(s) 873, 1034, 1147, 2049, 2167, 3129, 3201
TaiI ACGT 7 cut(s) 784, 1413, 1448, 1767, 2033, 2776, 2866
TaqI TCGA 8 cut(s) 109, 167, 600, 657, 1158, 2803, 3044, 3246
TatI WGTACW 2 cut(s) 2149, 3064
TfiI GAWTC 8 cut(s) 611, 696, 941, 1163, 1199, 1492, 2444, 2600
TscAI CASTG 9 cut(s) 255, 305, 448, 1191, 1264, 1477, 1864, 2245, 2285
TseFI GTSAC 6 cut(s) 1132, 1466, 1765, 2252, 2456, 2754
Tsp45I GTSAC 6 cut(s) 1132, 1466, 1765, 2252, 2456, 2754
TspGWI ACGGA 3 cut(s) 2594, 2810, 2923
TspRI CASTG 9 cut(s) 255, 305, 448, 1191, 1264, 1477, 1864, 2245, 2285
Van91I CCANNNNNTGG 2 cut(s) 161, 397
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 4 cut(s) 1039, 1546, 2065, 2931
VspI ATTAAT 1 cut(s) 2259
XceI RCATGY 6 cut(s) 1672, 1773, 2105, 2786, 3031, 3307
XcmI CCANNNNNNNNNTGG 1 cut(s) 1093
XmnI GAANNNNTTC 3 cut(s) 105, 453, 2536
XspI CTAG 6 cut(s) 519, 1917, 2145, 2153, 2399, 3228
Zsp2I ATGCAT 3 cut(s) 709, 1670, 2107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.