Rroxscaffold_2G00129840

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001949
Physical Location & Seq
Reverse (-)
65945338 .. 65956144
10807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00129840.1

Sequence Viewer

Length: 2952 bp
ATGAGAGGCTCAAGGCTGGAAGTGTTGATGTGTTGCACTGCTACTATGCCCATGGAGAAGACAATGAAAATTTCCCAAAGACGCAGTTATTGGATGCTTGAAGAGGACCTCTCGCACATTGTTCTCGTCCACTACCGGGAAGTAAAGGGAAATAGGACAAATTTCAATCATGTTAAAGAGACTGAAGGTATTCCCTATTCCAATGGAGCTGAAGAAATTGCCCGCCAGTCTGAGATGGAGAATTCTGTTTCTTCTAGTTTCAATCCAAGTAGTTACCAAATGCATTCTCAAGTTACGGACGCGACAAGCTTGAGTAGTGCTCAGGCATCAGAGTTCGAAGATGCCGAATCAGCATTTTATAACCAAGCAAGTTCCCGGTTGCAGCCAGTGGCTGAGAAAATTAATTCTGAGTTCTCTGATGCGTACTATCCTGCATTCTCAAATGATTTTCAAGAAAAGTTGTCAACCATTCCTGGGGTGGACTTTAGCTCACTCTCTCAAGCATATAAGAGGGAAGATGGTATTGATGCTGGAGATATCTATGAGCCCAGGAAAGATCGTGAATTTGCATTATGGGACGATATGGAAAACAGTTCTACTGTCATTCAATCTTTTCAGTCATCATTTTCAGCAACACATTCTGATACAATGGGAAGCTTTCCTAAGATTGAGACGATTGGGCATCTTTATACAGACAGTTTTGATAAAAGGCTCGTGTATGGGACTGAAAATAGACCAAAGGTCCAACAAAGTTGGCAGACTTCTGAAGGATCATCAAACTGGCCTATGGATCAGAGCTTGCAATCACATACAGAATACAATGTCACCGCTAAGCTCGATGAAGGAGCCGATACCACTGATTTACTCAAGTCCTTGGGGCCTTTCCTTATGGATTCAGACAAGAAAAATGACCTTCAATTTCATCTTTCAAACACAGATACTATTTCAAAGAGAAATGATAACATTGAGGGAAAAGCTGATCACCCTTCTGCTATTAAGCCTCTGTTGGGATTCGATGATGGTCTGAAGAAGCTCGACAGTTTCAACCGATGGATGAGTAAAGAACTTGAAGATGTGGATGAGCCACAAATGCAGTCCAGTTCTGGAGCCTACTGGGAAACTGTTGAAAGTGAGAATGAGGTTGATGAGTCCAGTGTTCCCCTGCAAGTACGCTTGGATAGCTATATGCTGGGGCCTTCTCTTTCTCATGACCAGCTCTTTAGCATTGTTGATTTCTCACCAAACTGGGCATATGAAAACTCTGAAATCAAGGTTTTGATCACAGGAAAATTTTTGAAAAGTCAACATGCAGAAAGTTGTAAATGGTCATGTATGTTTGGGGAAGTTGAAGTTCCTGCTGAGGTTATAGCAGATGGTGTTCTTCGTTGTTATACTCCCATACACAAGGCTGGAAGGGTTCCGTTTTATGTGACATGTTCCAATAGGCTGGCTTGTAGTGAAGTAAGGGAATTTGAATACCGGGTAGCCCAAATTCAAGATGTTGATTCTAAAGATTATTACAGTGACTGCTCAAATGAAACACTCAGTATGCGCTTCGGAAAATTATTGTCTTTAAGCTCTACCTCACCTAATTGTGATTCAGCAAGTACAGCAGAGAATTCTGAGTTGACCGCTAAAATTACTTCTATGCTGAAAAATGACAATGATGAATGGGACAAGATGCTACAACTTACGTCAGACGAGGACTTTTCCTTGGAAAGAGTAGAGGATCAGTTGCTTCAACAGTTACTTAAGGAGAAGTTGCATGCATGGCTCTTGCAGAAGCTGGCTGCTGGTGGTAAAGGCCCTAGTGTTCTAGACGAGGGTGGCCAAGGTGTGTTACATTTTGGAGCTGCTCTTGGCTATGACTGGGTCTTACTGCCCACAATAACTGCTGGAGTTAGCGTCAATTTTCGTGATGTGAATGGATGGACGGCACTTCATTGGGCAGCTTTTTGTGGCAGAGAGCGCACAGTCGCTTCTCTCATCTCCCTGGGTGCGGCTCCTGGAGCATTAACAGATCCAACTACCAAATATCCTTCAGGAGAGACACCTGCAGACCTAGCTTCTGAACAAGGACACAAAGGGATTGCTGGATATCTTGCAGAATCTGCTTTGAGTGCCCACCTTGAATCTCTTAATTTGGATCTCAAGAACCGAAATTCGGCAGAGACTTCAGGAACAAAAGCAGTTTCAGGTTCTAGCAGGGATGGGGAGTTAACGGATGGGCTTTCCCTGAGGGATTCTTTAACTGCTGTCTGTAATGCGACACAAGCTGCTGCTCGTATTCATCAAGTGTTCAGGGTACAATCTTTCCAAAGGAAGCAGTTGAAACAGTACGATGGTGATAAATTTGGATTTTCCAATGAACGGGCATTATCACTTATTGCAGTCAAGTCACATAAGGCAGGGAAACGTGATGAGCATGTGGATGCTGCAGCAGTACGAATACAAAATAAGTTCCGTAGTTGGAAGGGTAGAAAAGACTATTTGATAATCCGACAACGGATTGTCAAAATTCAGGCCCATGTAAGAGGTCACCAGGTGAGGAAAAACTATAGAAAGATAGTGTGGTCTGTCGGAATTGTAGAGAAGATCATCCTGCGTTGGAGAAGAAAGGGAAGTGGGTTGCGTGGGTTCAAGCCAGAACCTGTTACTGAGGGCCCCAGCATGCAAGTTTCATCCACAAAGGATGATGATGATGACTTACTGAAAGAAGGAAGGAAGCAAACAGAGGAAAGGCTGCAAAAGGCACTTGCTAGGGTGAAATCCATGGTTCAGTATCCTGAAGCAAGAGATCAATACCATCGACTTTTAAATGTTGTTACTGAGATTCAGGAAACCAAGGTGCTGAATAGTTCAGAAGGGACATCAGCTTATATGGATGATGACCTCATAGACATTGAAGCGTTGTTCGATGATGACGTTTTGATGCCTACAGCAGCTTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

983

Amino Acids

110.09

Weight (kDa)

5.22

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 616 - 704 7e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 360
AarI CACCTGC 1 cut(s) 2062
Acc36I ACCTGC 1 cut(s) 2062
AccII CGCG 1 cut(s) 302
AciI CCGC 4 cut(s) 223, 828, 1632, 2000
AclWI GGATC 5 cut(s) 778, 798, 1737, 2015, 2154
AcoI YGGCCR 1 cut(s) 1828
AcuI CTGAAG 7 cut(s) 204, 231, 786, 1046, 2025, 2160, 2808
AdeI CACNNNGTG 1 cut(s) 2545
AfaI GTAC 6 cut(s) 425, 1170, 1609, 2307, 2339, 2445
AfiI CCNNNNNNNGG 6 cut(s) 136, 474, 1007, 1999, 2164, 2370
AflII CTTAAG 1 cut(s) 1751
AflIII ACRYGT 1 cut(s) 1433
AhdI GACNNNNNGTC 1 cut(s) 740
AjnI CCWGG 5 cut(s) 472, 548, 1992, 2005, 2541
Alw21I GWGCWC 1 cut(s) 322
Alw26I GTCTC 4 cut(s) 173, 665, 2042, 2165
AlwI GGATC 5 cut(s) 778, 798, 1737, 2015, 2154
AlwNI CAGNNNCTG 5 cut(s) 392, 1527, 1786, 2111, 2651
AoxI GGCC 7 cut(s) 782, 878, 1193, 1804, 1828, 2523, 2662
ApaI GGGCCC 1 cut(s) 2666
AseI ATTAAT 1 cut(s) 402
Asp700I GAANNNNTTC 1 cut(s) 189
AspLEI GCGC 2 cut(s) 1554, 1971
AspS9I GGNCC 8 cut(s) 106, 742, 878, 1193, 1805, 2524, 2662, 2663
AsuC2I CCSGG 3 cut(s) 137, 376, 1481
AsuHPI GGTGA 8 cut(s) 817, 974, 1230, 1578, 2357, 2531, 2557, 2776
AsuII TTCGAA 1 cut(s) 336
AvaII GGWCC 2 cut(s) 106, 742
AxyI CCTNAGG 1 cut(s) 2237
BaeGI GKGCMC 2 cut(s) 2125, 2666
BalI TGGCCA 1 cut(s) 1830
BanII GRGCYC 2 cut(s) 549, 2666
BauI CACGAG 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 65
Bbv12I GWGCWC 1 cut(s) 322
BbvCI CCTCAGC 1 cut(s) 1359
BbvI GCAGC 9 cut(s) 394, 1777, 1840, 1961, 2263, 2266, 2422, 2450, 2730
BceAI ACGGC 1 cut(s) 1950
BciT130I CCWGG 5 cut(s) 474, 550, 1994, 2007, 2543
BciVI GTATCC 1 cut(s) 2793
BclI TGATCA 2 cut(s) 979, 1278
BcnI CCSGG 3 cut(s) 137, 376, 1481
BcoDI GTCTC 4 cut(s) 173, 665, 2042, 2165
BfaI CTAG 6 cut(s) 255, 1809, 1817, 2063, 2202, 2760
BfmI CTRYAG 4 cut(s) 2055, 2436, 2557, 2937
BfrI CTTAAG 1 cut(s) 1751
BfuAI ACCTGC 1 cut(s) 2062
BfuI GTATCC 1 cut(s) 2793
BlpI GCTNAGC 1 cut(s) 831
Bme1390I CCNGG 8 cut(s) 137, 376, 474, 550, 1481, 1994, 2007, 2543
Bme18I GGWCC 2 cut(s) 106, 742
BmeRI GACNNNNNGTC 1 cut(s) 740
BmgT120I GGNCC 8 cut(s) 106, 742, 878, 1193, 1805, 2524, 2662, 2663
BmiI GGNNCC 8 cut(s) 847, 879, 1108, 1194, 1419, 2004, 2664, 2665
BmrFI CCNGG 8 cut(s) 137, 376, 474, 550, 1481, 1994, 2007, 2543
BmrI ACTGGG 3 cut(s) 1123, 1255, 1879
BmsI GCATC 9 cut(s) 84, 331, 335, 409, 517, 691, 1671, 2422, 2922
BmuI ACTGGG 3 cut(s) 1123, 1255, 1879
BpiI GAAGAC 1 cut(s) 65
BplI GAGNNNNNCTC 4 cut(s) 95, 127, 304, 336
BpmI CTGGAG 4 cut(s) 552, 1125, 1917, 2028
Bpu10I CCTNAGC 2 cut(s) 321, 1359
Bpu1102I GCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 336
BpuEI CTTGAG 5 cut(s) 273, 331, 483, 851, 2135
BpuMI CCSGG 3 cut(s) 137, 376, 1481
BsaXI ACNNNNNCTCC 2 cut(s) 1890, 1920
Bsc4I CCNNNNNNNGG 6 cut(s) 136, 474, 1007, 1999, 2164, 2370
Bse1I ACTGG 8 cut(s) 226, 386, 785, 1098, 1118, 1152, 1250, 1874
Bse21I CCTNAGG 1 cut(s) 2237
BseBI CCWGG 5 cut(s) 474, 550, 1994, 2007, 2543
BseLI CCNNNNNNNGG 6 cut(s) 136, 474, 1007, 1999, 2164, 2370
BseNI ACTGG 8 cut(s) 226, 386, 785, 1098, 1118, 1152, 1250, 1874
BseSI GKGCMC 2 cut(s) 2125, 2666
BseXI GCAGC 9 cut(s) 394, 1777, 1840, 1961, 2263, 2266, 2422, 2450, 2730
BseYI CCCAGC 2 cut(s) 1189, 2666
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 7 cut(s) 784, 880, 1195, 1806, 1830, 2525, 2664
BsiHKAI GWGCWC 1 cut(s) 322
BsiSI CCGG 3 cut(s) 136, 376, 1480
BslFI GGGAC 4 cut(s) 590, 736, 1688, 2881
BslI CCNNNNNNNGG 6 cut(s) 136, 474, 1007, 1999, 2164, 2370
BsmAI GTCTC 4 cut(s) 173, 665, 2042, 2165
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 4 cut(s) 590, 736, 1688, 2881
BsmI GAATGC 2 cut(s) 283, 434
BsnI GGCC 7 cut(s) 784, 880, 1195, 1806, 1830, 2525, 2664
Bsp119I TTCGAA 1 cut(s) 336
Bsp120I GGGCCC 1 cut(s) 2662
Bsp1286I GDGCHC 4 cut(s) 322, 549, 2125, 2666
Bsp1720I GCTNAGC 1 cut(s) 831
Bsp19I CCATGG 2 cut(s) 51, 2772
BspACI CCGC 4 cut(s) 223, 828, 1632, 2000
BspANI GGCC 7 cut(s) 784, 880, 1195, 1806, 1830, 2525, 2664
BspFNI CGCG 1 cut(s) 302
BspHI TCATGA 1 cut(s) 1207
BspLI GGNNCC 8 cut(s) 847, 879, 1108, 1194, 1419, 2004, 2664, 2665
BspMAI CTGCAG 2 cut(s) 2059, 2440
BspMI ACCTGC 1 cut(s) 2062
BspPI GGATC 5 cut(s) 778, 798, 1737, 2015, 2154
BspT104I TTCGAA 1 cut(s) 336
BspTI CTTAAG 1 cut(s) 1751
BsrI ACTGG 8 cut(s) 226, 386, 785, 1098, 1118, 1152, 1250, 1874
BssSI CACGAG 1 cut(s) 713
BssT1I CCWWGG 6 cut(s) 51, 873, 1713, 1831, 2772, 2844
Bst2BI CACGAG 1 cut(s) 713
Bst2UI CCWGG 5 cut(s) 474, 550, 1994, 2007, 2543
Bst4CI ACNGT 9 cut(s) 593, 601, 698, 1040, 1123, 1523, 1746, 1975, 2337
Bst6I CTCTTC 1 cut(s) 96
BstAFI CTTAAG 1 cut(s) 1751
BstAPI GCANNNNNTGC 1 cut(s) 2111
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 6 cut(s) 223, 800, 1449, 1767, 1788, 2672
BstDSI CCRYGG 2 cut(s) 51, 2772
BstEII GGTNACC 1 cut(s) 2537
BstFNI CGCG 1 cut(s) 302
BstHHI GCGC 2 cut(s) 1554, 1971
BstMAI GTCTC 4 cut(s) 173, 665, 2042, 2165
BstNI CCWGG 5 cut(s) 474, 550, 1994, 2007, 2543
BstNSI RCATGY 5 cut(s) 1310, 1437, 1769, 2429, 2674
BstPI GGTNACC 1 cut(s) 2537
BstSCI CCNGG 8 cut(s) 135, 374, 472, 548, 1479, 1992, 2005, 2541
BstSFI CTRYAG 4 cut(s) 2055, 2436, 2557, 2937
BstSLI GKGCMC 2 cut(s) 2125, 2666
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 9 cut(s) 394, 1777, 1840, 1961, 2263, 2266, 2422, 2450, 2730
BstV2I GAAGAC 1 cut(s) 65
BstX2I RGATCY 2 cut(s) 2020, 2146
BstXI CCANNNNNNTGG 1 cut(s) 1447
BstYI RGATCY 2 cut(s) 2020, 2146
Bsu36I CCTNAGG 1 cut(s) 2237
BsuI GTATCC 1 cut(s) 2793
BsuRI GGCC 7 cut(s) 784, 880, 1195, 1806, 1830, 2525, 2664
BtgI CCRYGG 2 cut(s) 51, 2772
BtsI GCAGTG 1 cut(s) 36
BtsIMutI CAGTG 5 cut(s) 36, 393, 855, 1159, 1528
BveI ACCTGC 1 cut(s) 2062
Cac8I GCNNGC 6 cut(s) 223, 800, 1449, 1767, 1788, 2672
CaiI CAGNNNCTG 5 cut(s) 392, 1527, 1786, 2111, 2651
CciI TCATGA 1 cut(s) 1207
CfoI GCGC 2 cut(s) 1554, 1971
Cfr13I GGNCC 8 cut(s) 106, 742, 878, 1193, 1805, 2524, 2662, 2663
CseI GACGC 3 cut(s) 90, 308, 1894
CsiI ACCWGGT 1 cut(s) 2541
Csp6I GTAC 6 cut(s) 424, 1169, 1608, 2306, 2338, 2444
CviQI GTAC 6 cut(s) 424, 1169, 1608, 2306, 2338, 2444
DraI TTTAAA 1 cut(s) 2817
DraIII CACNNNGTG 1 cut(s) 2545
DriI GACNNNNNGTC 1 cut(s) 740
EaeI YGGCCR 1 cut(s) 1828
Eam1104I CTCTTC 1 cut(s) 96
Eam1105I GACNNNNNGTC 1 cut(s) 740
EarI CTCTTC 1 cut(s) 96
Eco130I CCWWGG 6 cut(s) 51, 873, 1713, 1831, 2772, 2844
Eco24I GRGCYC 2 cut(s) 549, 2666
Eco32I GATATC 2 cut(s) 538, 2099
Eco47I GGWCC 2 cut(s) 106, 742
Eco57I CTGAAG 7 cut(s) 204, 231, 786, 1046, 2025, 2160, 2808
Eco81I CCTNAGG 1 cut(s) 2237
Eco91I GGTNACC 1 cut(s) 2537
EcoO109I RGGNCCY 6 cut(s) 106, 878, 1193, 1805, 2662, 2663
EcoO65I GGTNACC 1 cut(s) 2537
EcoRI GAATTC 2 cut(s) 241, 1618
EcoRII CCWGG 5 cut(s) 472, 548, 1992, 2005, 2541
EcoRV GATATC 2 cut(s) 538, 2099
EcoT14I CCWWGG 6 cut(s) 51, 873, 1713, 1831, 2772, 2844
EcoT22I ATGCAT 2 cut(s) 285, 1771
EcoT38I GRGCYC 2 cut(s) 549, 2666
ErhI CCWWGG 6 cut(s) 51, 873, 1713, 1831, 2772, 2844
Esp3I CGTCTC 1 cut(s) 665
FaqI GGGAC 4 cut(s) 590, 736, 1688, 2881
FauI CCCGC 1 cut(s) 230
FauNDI CATATG 1 cut(s) 1252
FbaI TGATCA 2 cut(s) 979, 1278
FriOI GRGCYC 2 cut(s) 549, 2666
FspBI CTAG 6 cut(s) 255, 1809, 1817, 2063, 2202, 2760
GlaI GCGC 2 cut(s) 1553, 1970
GsaI CCCAGC 2 cut(s) 1193, 2670
GsuI CTGGAG 4 cut(s) 552, 1125, 1917, 2028
HaeIII GGCC 7 cut(s) 784, 880, 1195, 1806, 1830, 2525, 2664
HapII CCGG 3 cut(s) 136, 376, 1480
HgaI GACGC 3 cut(s) 90, 308, 1894
HhaI GCGC 2 cut(s) 1554, 1971
Hin6I GCGC 2 cut(s) 1552, 1969
HinP1I GCGC 2 cut(s) 1552, 1969
HincII GTYRAC 4 cut(s) 465, 1304, 1629, 2220
HindII GTYRAC 4 cut(s) 465, 1304, 1629, 2220
HindIII AAGCTT 2 cut(s) 307, 655
HpaI GTTAAC 1 cut(s) 2220
HpaII CCGG 3 cut(s) 136, 376, 1480
HphI GGTGA 8 cut(s) 817, 974, 1230, 1578, 2357, 2531, 2557, 2776
Hpy166II GTNNAC 6 cut(s) 130, 465, 481, 1304, 1629, 2220
Hpy8I GTNNAC 6 cut(s) 130, 465, 481, 1304, 1629, 2220
HpyCH4III ACNGT 9 cut(s) 593, 601, 698, 1040, 1123, 1523, 1746, 1975, 2337
HpyCH4IV ACGT 3 cut(s) 1694, 2416, 2925
HpySE526I ACGT 3 cut(s) 1694, 2416, 2925
HspAI GCGC 2 cut(s) 1552, 1969
Ksp22I TGATCA 2 cut(s) 979, 1278
KspAI GTTAAC 1 cut(s) 2220
LmnI GCTCC 6 cut(s) 206, 845, 1106, 1850, 2008, 2009
Lsp1109I GCAGC 9 cut(s) 394, 1777, 1840, 1961, 2263, 2266, 2422, 2450, 2730
LweI GCATC 9 cut(s) 84, 331, 335, 409, 517, 691, 1671, 2422, 2922
MabI ACCWGGT 1 cut(s) 2541
MaeI CTAG 6 cut(s) 255, 1809, 1817, 2063, 2202, 2760
MaeII ACGT 3 cut(s) 1694, 2416, 2925
MflI RGATCY 2 cut(s) 2020, 2146
MhlI GDGCHC 4 cut(s) 322, 549, 2125, 2666
MlsI TGGCCA 1 cut(s) 1830
MluNI TGGCCA 1 cut(s) 1830
MlyI GAGTC 1 cut(s) 1157
MmeI TCCRAC 6 cut(s) 769, 2048, 2450, 2524, 2560, 2588
Mox20I TGGCCA 1 cut(s) 1830
Mph1103I ATGCAT 2 cut(s) 285, 1771
MroXI GAANNNNTTC 1 cut(s) 189
MscI TGGCCA 1 cut(s) 1830
MslI CAYNNNNRTG 1 cut(s) 2430
Msp20I TGGCCA 1 cut(s) 1830
MspCI CTTAAG 1 cut(s) 1751
MspI CCGG 3 cut(s) 136, 376, 1480
MspR9I CCNGG 8 cut(s) 137, 376, 474, 550, 1481, 1994, 2007, 2543
Mva1269I GAATGC 2 cut(s) 283, 434
MvaI CCWGG 5 cut(s) 474, 550, 1994, 2007, 2543
MvnI CGCG 1 cut(s) 302
NciI CCSGG 3 cut(s) 137, 376, 1481
NcoI CCATGG 2 cut(s) 51, 2772
NdeI CATATG 1 cut(s) 1252
NlaIV GGNNCC 8 cut(s) 847, 879, 1108, 1194, 1419, 2004, 2664, 2665
NmuCI GTSAC 5 cut(s) 823, 1429, 1523, 2397, 2537
NsiI ATGCAT 2 cut(s) 285, 1771
NspI RCATGY 5 cut(s) 1310, 1437, 1769, 2429, 2674
NspV TTCGAA 1 cut(s) 336
PaeI GCATGC 2 cut(s) 1769, 2674
PagI TCATGA 1 cut(s) 1207
PaqCI CACCTGC 1 cut(s) 2062
PasI CCCWGGG 1 cut(s) 1993
PciI ACATGT 1 cut(s) 1433
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 2922
PctI GAATGC 2 cut(s) 283, 434
PdmI GAANNNNTTC 1 cut(s) 189
PfeI GAWTC 9 cut(s) 347, 893, 1011, 1505, 1598, 2108, 2132, 2243, 2833
PflFI GACNNNGTC 1 cut(s) 1871
PfoI TCCNGGA 1 cut(s) 2005
PleI GAGTC 1 cut(s) 1156
PpsI GAGTC 1 cut(s) 1156
PpuMI RGGWCCY 1 cut(s) 106
PscI ACATGT 1 cut(s) 1433
PshBI ATTAAT 1 cut(s) 402
PsiI TTATAA 1 cut(s) 360
Psp5II RGGWCCY 1 cut(s) 106
Psp6I CCWGG 5 cut(s) 472, 548, 1992, 2005, 2541
PspEI GGTNACC 1 cut(s) 2537
PspFI CCCAGC 2 cut(s) 1189, 2666
PspGI CCWGG 5 cut(s) 472, 548, 1992, 2005, 2541
PspN4I GGNNCC 8 cut(s) 847, 879, 1108, 1194, 1419, 2004, 2664, 2665
PspOMI GGGCCC 1 cut(s) 2662
PspPI GGNCC 8 cut(s) 106, 742, 878, 1193, 1805, 2524, 2662, 2663
PspPPI RGGWCCY 1 cut(s) 106
PstI CTGCAG 2 cut(s) 2059, 2440
PstNI CAGNNNCTG 5 cut(s) 392, 1527, 1786, 2111, 2651
PsuI RGATCY 2 cut(s) 2020, 2146
PsyI GACNNNGTC 1 cut(s) 1871
RsaI GTAC 6 cut(s) 425, 1170, 1609, 2307, 2339, 2445
RsaNI GTAC 6 cut(s) 424, 1169, 1608, 2306, 2338, 2444
RseI CAYNNNNRTG 1 cut(s) 2430
Sau96I GGNCC 8 cut(s) 106, 742, 878, 1193, 1805, 2524, 2662, 2663
SchI GAGTC 1 cut(s) 1157
ScrFI CCNGG 8 cut(s) 137, 376, 474, 550, 1481, 1994, 2007, 2543
SduI GDGCHC 4 cut(s) 322, 549, 2125, 2666
SexAI ACCWGGT 1 cut(s) 2541
SfaNI GCATC 9 cut(s) 84, 331, 335, 409, 517, 691, 1671, 2422, 2922
SfcI CTRYAG 4 cut(s) 2055, 2436, 2557, 2937
SfuI TTCGAA 1 cut(s) 336
SinI GGWCC 2 cut(s) 106, 742
SmiMI CAYNNNNRTG 1 cut(s) 2430
SmlI CTYRAG 7 cut(s) 10, 288, 310, 498, 866, 1751, 2150
SmoI CTYRAG 7 cut(s) 10, 288, 310, 498, 866, 1751, 2150
SphI GCATGC 2 cut(s) 1769, 2674
SsiI CCGC 4 cut(s) 223, 828, 1632, 2000
SspMI CTAG 6 cut(s) 255, 1809, 1817, 2063, 2202, 2760
StyD4I CCNGG 8 cut(s) 135, 374, 472, 548, 1479, 1992, 2005, 2541
StyI CCWWGG 6 cut(s) 51, 873, 1713, 1831, 2772, 2844
TaaI ACNGT 9 cut(s) 593, 601, 698, 1040, 1123, 1523, 1746, 1975, 2337
TaiI ACGT 3 cut(s) 1697, 2419, 2928
TaqI TCGA 6 cut(s) 336, 837, 1014, 1035, 2809, 2916
TatI WGTACW 1 cut(s) 1607
TauI GCSGC 1 cut(s) 2003
TfiI GAWTC 9 cut(s) 347, 893, 1011, 1505, 1598, 2108, 2132, 2243, 2833
TscAI CASTG 5 cut(s) 43, 393, 862, 1159, 1528
TseFI GTSAC 5 cut(s) 823, 1429, 1523, 2397, 2537
Tsp45I GTSAC 5 cut(s) 823, 1429, 1523, 2397, 2537
TspGWI ACGGA 5 cut(s) 311, 1410, 2237, 2453, 2521
TspRI CASTG 5 cut(s) 43, 393, 862, 1159, 1528
Tth111I GACNNNGTC 1 cut(s) 1871
Vha464I CTTAAG 1 cut(s) 1751
VpaK11BI GGWCC 2 cut(s) 106, 742
VspI ATTAAT 1 cut(s) 402
XbaI TCTAGA 1 cut(s) 1816
XceI RCATGY 5 cut(s) 1310, 1437, 1769, 2429, 2674
XcmI CCANNNNNNNNNTGG 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 189
XspI CTAG 6 cut(s) 255, 1809, 1817, 2063, 2202, 2760
Zsp2I ATGCAT 2 cut(s) 285, 1771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.