RLG00000018051

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001949
Physical Location & Seq
Reverse (-)
24860640 .. 24867031
6392 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018051

Sequence Viewer

Length: 2685 bp
ATGGCGGCTAGGAAGCGACAGCGTCTTAATGATGAATTAGGCCTTCCGGAAATACTTTTACAAGCAAAGCATCGATGGCTACGTCCTGCTGAAATTTTCGAAGTTCTTCAAAATCATTCAAGATTTTGTCTTTCGGAGCCTGCAGATAAGCCATCGAGTGGATCATTGTATCTTTATGATCGGAAGGTGCTCAGGTCTTTCAGAAAAGATGGCTATAGATGGAAGAAGACAAAAGATGGAAAGGCAGTGAAGGAAGCCCATGAGAGGCTCAAGGCTAAAGGAGTTGAGGCATTACATTGCTACTATGCCCATGGAGAAGAAAATGAGAATTTCCAAAGACGCTGTTATTGGATGATTGAAGAGCCTCTCTCGCACATAGTTCTGGTCCACTACCGAGAAGTAAAGACTTCTGAAGGTAACTTGTCATGTTCATCCAAGTTGTCTATCAATTGGAATATGCATTCAGATACTGCATATCATGCCAGTACTTCATTCCCTGGAGGAGTCAATGCAGACGAGTTAATCAATTCCCTGGGGCCTCCTTGCCATATGAATTTGGATAAACAAAATGATTCTTCTGTGCATAGGCACTCTGATAAGCAAAATGACCCTCGAATATTGAAAAATCTTGACAGTTCTGATCAATCAAGTGAGAATGGGAATGATGGGTCCGGTGCTCTTCAGCCAGTTCAAAGAGCTATTCCAGGACCTTCTCTATTCCAGGACCTTCTCTTTAACATTATTGATCTTTCACCAAACTGGGCATATGAAAACTCTGAAACAAAGGTTCTTGTTGTGGGAAAGTTGTTGAAGAATCAAGAATTAGAAAGTTTTAAGTGGTCATGTATGTTTGGGGAAGTAGAAGTCCCCGCTGAGGTTGTAGCAGATGGTGTTCTTCGTTGTTATACGCCCGTACATAGGGCTGCTAGGATTCCTTTCTATGTAACACGTTCCAATAGGTTAGCGTGTAGTGAAGTGAGAATATTTGAGTACCGAGTCAACCAAATTCAAGATGCGGATTTTAAGGATGACGGCTGCGAAGACGATACACTTGTTATGCGTTTTGGAAGAATACTGTCCCCAAGCTTTACGTATTCAAGTTTTAATCCACAGGATAAATATAGACAATATGATCCTATCAGTGTAGTTGAGAATTCTGATTTGACTAGTAAAATTAGTTCATTGCTAAAGAACGGCAATGAGGAAAGAGCTGTGATGCTAAAGCTATTTCCAGATTTGTTTTCCTTGGATACAGTAAAGGTGCAGTTGCTTCAACAGTTTCTTAAAGACAAGTTTCATGAATGGCTCTTGCAGAAGCTGGCTGTTGGTGGAAATGGCTTTAGGGTACTAGACGAAGGTGGCCAGGGTGTGTTACATGTTGGAGCTGCTCTTGGCTATGACTGGGTCTTTTTACCCATGACAATGGCTGGAGTTACTGTCAATTTTCCTGATGTGAATGGATGGACGGCACTTCATTGGGCAGCTTTTCGTGGCAGCGCAGTTTCTTTGCTCATCGGACTTGGTGCAGCTCCTGGAGCATTAACAGATCCATGTACCAAAAGCCCTACAGGCAAAACACCAGCGGACCTAGCTTCTGAAAAGGGGTACAAAGGAATAGCTGCTTATCTTTCATCACTTATATTGGATATCAAGGATGCAAATTCTGCAGAGAGTTCAAGAGAAAAATTAGTCCAACCAGTTCCAGAACGAATTCCCACTTCTAGCAGCGATGGGGATTTAGCTGATGGGCTTTCCATGAGGGTTTGTCTAAATACTGTTTGTAATGCTACCCAAGCTGCTGCTCATATAACAGGTCGGCAGGTGAAGAAAAACTATGGTATGGGCTGGGCCGTAAGAACTGTGGGGATGATATTGCGTTGGAGAGGACGGGGAAAACGAAGAAGGTTGCGTCGATTAGAGCTAGAACTACTTAGTGAGGGCCCTTATAATATTAGTTATGACTTTCTAAAGATTCAGGCCCATGCAACAGGTCACCAGGTGAAGAAAAACTATGAGCTAGAACTATTTAGTGAGAGCCCTTATGATTATGACTTTCAAAATTCAGGCTCATATAACAGGTCACCAGGTGAAGAAAACTATGGTATGGGCTGGGCCGTAAGAACTGTGGGGATGATTTTGCGTTGGAGAGGACGAGGAAAACGAAGAAGGTTGCGTCGGATTCCAAGATGGCTCCAGGAATTTCCATCAACAAAGTCAGCAGCAAATTCATGGAATGCAGTCACAGTTGCAGCCACAGCACCAGAACCCTGTTTGACTCCAACAAATATTGAGACAAATGCACATGCAGCAAATGTTGATTCGGCAAATGCAGCTATCTGCCCAATCCAAGAATGTGTCATTTCAGCAACAGCTGCACATAGAAAGAATGCGAAGACGTCAACCATCTACTCCTCGTGCTGCTATGGACATGGTACAGGACAGACCAATGGCCGGTCCAGGCAGCAGCAAATTCCTGCAATGTCGAATCCTACAATGCAAAATGTCCCTGCTCAGTCAGGGAATCAGTTTAGACAGATTTCTTCACAAATTGCACAAATTCAATCACAGAACGCCGGTGTTCTCGTGGCTCGACCAGTGATGGTTGAAGCTTTCCTGGAATTAATGGGTGCAGGTGCTTCATCGAAGCATCATTCGGATGAAAATAGGCTGACCTCCTCAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

895

Amino Acids

99.76

Weight (kDa)

8.85

Isoelectric Point (pI)

48.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 21 - 133 1.5e-44 CG-1 domain
Ank_2 PF12796 474 - 545 1.8e-06 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1947
AarI CACCTGC 2 cut(s) 1810, 2621
AatII GACGTC 1 cut(s) 2399
Acc36I ACCTGC 2 cut(s) 1810, 2621
AccB7I CCANNNNNTGG 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 46
AciI CCGC 4 cut(s) 5, 870, 1016, 1583
AclWI GGATC 3 cut(s) 169, 1127, 1541
AcoI YGGCCR 2 cut(s) 1360, 2449
AcuI CTGAAG 2 cut(s) 432, 665
AcyI GRCGYC 1 cut(s) 2396
AdeI CACNNNGTG 2 cut(s) 1999, 2087
AfaI GTAC 7 cut(s) 487, 915, 992, 1347, 1555, 1607, 2434
AfiI CCNNNNNNNGG 5 cut(s) 158, 264, 874, 918, 2451
AflIII ACRYGT 2 cut(s) 947, 1375
AhlI ACTAGT 1 cut(s) 1166
Alw21I GWGCWC 2 cut(s) 192, 679
Alw26I GTCTC 1 cut(s) 2285
AlwI GGATC 3 cut(s) 169, 1127, 1541
AlwNI CAGNNNCTG 3 cut(s) 470, 1318, 1532
Aor13HI TCCGGA 1 cut(s) 46
AoxI GGCC 8 cut(s) 40, 536, 1360, 1848, 1939, 1977, 2112, 2449
ApaI GGGCCC 1 cut(s) 1943
AseI ATTAAT 1 cut(s) 2621
Asp700I GAANNNNTTC 2 cut(s) 105, 1710
AspLEI GCGC 1 cut(s) 1499
AsuHPI GGTGA 6 cut(s) 744, 1834, 1985, 2011, 2073, 2099
AsuII TTCGAA 1 cut(s) 99
AvaII GGWCC 6 cut(s) 385, 669, 707, 724, 1585, 2454
BaeGI GKGCMC 1 cut(s) 1943
BaeI ACNNNNGTAYC 2 cut(s) 1038, 1071
BalI TGGCCA 1 cut(s) 1362
BanII GRGCYC 2 cut(s) 1943, 2039
BauI CACGAG 2 cut(s) 2413, 2582
BbsI GAAGAC 3 cut(s) 233, 1047, 2399
Bbv12I GWGCWC 2 cut(s) 192, 679
BbvCI CCTCAGC 1 cut(s) 873
BceAI ACGGC 5 cut(s) 1048, 1210, 1482, 1835, 2099
BciVI GTATCC 1 cut(s) 1243
BclI TGATCA 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 2285
BcuI ACTAGT 1 cut(s) 1166
BfaI CTAG 8 cut(s) 9, 927, 1167, 1349, 1589, 1722, 1922, 2018
BfmI CTRYAG 4 cut(s) 141, 214, 1566, 1665
BfuAI ACCTGC 2 cut(s) 1810, 2621
BfuI GTATCC 1 cut(s) 1243
BglI GCCNNNNNGGC 1 cut(s) 1569
BmcAI AGTACT 1 cut(s) 487
Bme18I GGWCC 6 cut(s) 385, 669, 707, 724, 1585, 2454
BmiI GGNNCC 5 cut(s) 138, 537, 670, 1941, 2192
BmrI ACTGGG 2 cut(s) 769, 1411
BmsI GCATC 5 cut(s) 79, 1003, 1206, 1645, 2656
BmuI ACTGGG 2 cut(s) 769, 1411
BpiI GAAGAC 3 cut(s) 233, 1047, 2399
BplI GAGNNNNNCTC 2 cut(s) 353, 385
BpmI CTGGAG 4 cut(s) 519, 1449, 1554, 2177
Bpu10I CCTNAGC 2 cut(s) 191, 873
Bpu14I TTCGAA 1 cut(s) 99
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 73
BsaAI YACGTR 1 cut(s) 1092
BsaHI GRCGYC 1 cut(s) 2396
BsaJI CCNNGG 6 cut(s) 310, 496, 531, 532, 1245, 1363
BsaWI WCCGGW 2 cut(s) 46, 671
BsaXI ACNNNNNCTCC 2 cut(s) 1422, 1452
Bsc4I CCNNNNNNNGG 5 cut(s) 158, 264, 874, 918, 2451
Bse118I RCCGGY 2 cut(s) 2451, 2573
Bse1I ACTGG 6 cut(s) 483, 686, 764, 1406, 1697, 2594
Bse3DI GCAATG 4 cut(s) 295, 1181, 1204, 2484
BseAI TCCGGA 1 cut(s) 46
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 6 cut(s) 310, 496, 531, 532, 1245, 1363
BseGI GGATG 8 cut(s) 357, 431, 1033, 1466, 1660, 1872, 2136, 2662
BseLI CCNNNNNNNGG 5 cut(s) 158, 264, 874, 918, 2451
BseMI GCAATG 4 cut(s) 295, 1181, 1204, 2484
BseMII CTCAG 3 cut(s) 205, 864, 2525
BseNI ACTGG 6 cut(s) 483, 686, 764, 1406, 1697, 2594
BseRI GAGGAG 3 cut(s) 516, 2401, 2665
BseSI GKGCMC 1 cut(s) 1943
BseYI CCCAGC 2 cut(s) 1845, 2109
BsgI GTGCAG 4 cut(s) 1283, 1545, 2358, 2649
BshFI GGCC 8 cut(s) 42, 538, 1362, 1850, 1941, 1979, 2114, 2451
BshVI ATCGAT 1 cut(s) 73
BsiHKAI GWGCWC 2 cut(s) 192, 679
BsiSI CCGG 4 cut(s) 47, 672, 2452, 2574
BslFI GGGAC 3 cut(s) 851, 1063, 2489
BslI CCNNNNNNNGG 5 cut(s) 158, 264, 874, 918, 2451
BsmAI GTCTC 1 cut(s) 2285
BsmFI GGGAC 3 cut(s) 851, 1063, 2489
BsmI GAATGC 3 cut(s) 460, 2239, 2392
BsnI GGCC 8 cut(s) 42, 538, 1362, 1850, 1941, 1979, 2114, 2451
Bsp119I TTCGAA 1 cut(s) 99
Bsp120I GGGCCC 1 cut(s) 1939
Bsp1286I GDGCHC 4 cut(s) 192, 679, 1943, 2039
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
Bsp19I CCATGG 1 cut(s) 310
BspACI CCGC 4 cut(s) 5, 870, 1016, 1583
BspANI GGCC 8 cut(s) 42, 538, 1362, 1850, 1941, 1979, 2114, 2451
BspCNI CTCAG 3 cut(s) 204, 865, 2524
BspDI ATCGAT 1 cut(s) 73
BspEI TCCGGA 1 cut(s) 46
BspHI TCATGA 1 cut(s) 1297
BspLI GGNNCC 5 cut(s) 138, 537, 670, 1941, 2192
BspMAI CTGCAG 2 cut(s) 145, 1669
BspMI ACCTGC 2 cut(s) 1810, 2621
BspPI GGATC 3 cut(s) 169, 1127, 1541
BspQI GCTCTTC 2 cut(s) 354, 684
BspT104I TTCGAA 1 cut(s) 99
BsrDI GCAATG 4 cut(s) 295, 1181, 1204, 2484
BsrFI RCCGGY 2 cut(s) 2451, 2573
BsrI ACTGG 6 cut(s) 483, 686, 764, 1406, 1697, 2594
BssAI RCCGGY 2 cut(s) 2451, 2573
BssECI CCNNGG 6 cut(s) 310, 496, 531, 532, 1245, 1363
BssMI GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
BssNI GRCGYC 1 cut(s) 2396
BssSI CACGAG 2 cut(s) 2413, 2582
BssT1I CCWWGG 2 cut(s) 310, 1245
Bst2BI CACGAG 2 cut(s) 2413, 2582
Bst4CI ACNGT 9 cut(s) 635, 1077, 1255, 1278, 1438, 1777, 1861, 2125, 2245
Bst6I CTCTTC 2 cut(s) 354, 684
BstACI GRCGYC 1 cut(s) 2396
BstAPI GCANNNNNTGC 3 cut(s) 479, 1664, 2372
BstBAI YACGTR 1 cut(s) 1092
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 2 cut(s) 141, 1320
BstDEI CTNAG 4 cut(s) 191, 873, 1931, 2511
BstDSI CCRYGG 1 cut(s) 310
BstEII GGTNACC 2 cut(s) 1991, 2079
BstF5I GGATG 8 cut(s) 357, 431, 1033, 1466, 1660, 1872, 2136, 2662
BstHHI GCGC 1 cut(s) 1499
BstKTI GATC 6 cut(s) 164, 181, 643, 748, 1135, 1549
BstMAI GTCTC 1 cut(s) 2285
BstMBI GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
BstNSI RCATGY 2 cut(s) 1379, 2306
BstPI GGTNACC 2 cut(s) 1991, 2079
BstSFI CTRYAG 4 cut(s) 141, 214, 1566, 1665
BstSLI GKGCMC 1 cut(s) 1943
BstSNI TACGTA 1 cut(s) 1092
BstV2I GAAGAC 3 cut(s) 233, 1047, 2399
BstX2I RGATCY 1 cut(s) 1546
BstXI CCANNNNNNTGG 1 cut(s) 1423
BstYI RGATCY 1 cut(s) 1546
Bsu15I ATCGAT 1 cut(s) 73
BsuI GTATCC 1 cut(s) 1243
BsuRI GGCC 8 cut(s) 42, 538, 1362, 1850, 1941, 1979, 2114, 2451
BsuTUI ATCGAT 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 310
BtgZI GCGATG 1 cut(s) 1743
BtsCI GGATG 8 cut(s) 357, 431, 1033, 1466, 1660, 1872, 2136, 2662
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 3 cut(s) 252, 1147, 2601
BveI ACCTGC 2 cut(s) 1810, 2621
Cac8I GCNNGC 2 cut(s) 141, 1320
CaiI CAGNNNCTG 3 cut(s) 470, 1318, 1532
CciI TCATGA 1 cut(s) 1297
CfoI GCGC 1 cut(s) 1499
Cfr10I RCCGGY 2 cut(s) 2451, 2573
ClaI ATCGAT 1 cut(s) 73
CseI GACGC 4 cut(s) 11, 348, 1898, 2162
CsiI ACCWGGT 2 cut(s) 1995, 2083
Csp6I GTAC 7 cut(s) 486, 914, 991, 1346, 1554, 1606, 2433
CviQI GTAC 7 cut(s) 486, 914, 991, 1346, 1554, 1606, 2433
DdeI CTNAG 4 cut(s) 191, 873, 1931, 2511
DpnI GATC 6 cut(s) 163, 180, 642, 747, 1134, 1548
DpnII GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
DraIII CACNNNGTG 2 cut(s) 1999, 2087
EaeI YGGCCR 2 cut(s) 1360, 2449
Eam1104I CTCTTC 2 cut(s) 354, 684
EarI CTCTTC 2 cut(s) 354, 684
Eco105I TACGTA 1 cut(s) 1092
Eco130I CCWWGG 2 cut(s) 310, 1245
Eco147I AGGCCT 1 cut(s) 42
Eco24I GRGCYC 2 cut(s) 1943, 2039
Eco32I GATATC 1 cut(s) 1648
Eco47I GGWCC 6 cut(s) 385, 669, 707, 724, 1585, 2454
Eco57I CTGAAG 2 cut(s) 432, 665
Eco91I GGTNACC 2 cut(s) 1991, 2079
EcoO109I RGGNCCY 5 cut(s) 536, 707, 724, 1939, 1940
EcoO65I GGTNACC 2 cut(s) 1991, 2079
EcoRI GAATTC 2 cut(s) 1153, 1710
EcoRV GATATC 1 cut(s) 1648
EcoT14I CCWWGG 2 cut(s) 310, 1245
EcoT22I ATGCAT 1 cut(s) 462
EcoT38I GRGCYC 2 cut(s) 1943, 2039
ErhI CCWWGG 2 cut(s) 310, 1245
FaqI GGGAC 3 cut(s) 851, 1063, 2489
FauI CCCGC 1 cut(s) 877
FauNDI CATATG 2 cut(s) 549, 766
FbaI TGATCA 1 cut(s) 640
FokI GGATG 8 cut(s) 364, 418, 1040, 1473, 1667, 1879, 2143, 2669
FriOI GRGCYC 2 cut(s) 1943, 2039
FspBI CTAG 8 cut(s) 9, 927, 1167, 1349, 1589, 1722, 1922, 2018
GlaI GCGC 1 cut(s) 1498
GsaI CCCAGC 2 cut(s) 1849, 2113
GsuI CTGGAG 4 cut(s) 519, 1449, 1554, 2177
HaeIII GGCC 8 cut(s) 42, 538, 1362, 1850, 1941, 1979, 2114, 2451
HapII CCGG 4 cut(s) 47, 672, 2452, 2574
HgaI GACGC 4 cut(s) 11, 348, 1898, 2162
HhaI GCGC 1 cut(s) 1499
Hin1I GRCGYC 1 cut(s) 2396
Hin6I GCGC 1 cut(s) 1497
HinP1I GCGC 1 cut(s) 1497
HincII GTYRAC 2 cut(s) 1000, 2400
HindII GTYRAC 2 cut(s) 1000, 2400
HindIII AAGCTT 2 cut(s) 1084, 2607
HpaII CCGG 4 cut(s) 47, 672, 2452, 2574
HphI GGTGA 6 cut(s) 744, 1834, 1985, 2011, 2073, 2099
Hpy166II GTNNAC 3 cut(s) 388, 1000, 2400
Hpy8I GTNNAC 3 cut(s) 388, 1000, 2400
Hpy99I CGWCG 2 cut(s) 1914, 2178
HpyAV CCTTC 9 cut(s) 53, 178, 244, 407, 720, 737, 1349, 1896, 2160
HpyCH4III ACNGT 9 cut(s) 635, 1077, 1255, 1278, 1438, 1777, 1861, 2125, 2245
HpyCH4IV ACGT 4 cut(s) 82, 949, 1091, 2396
HpyF3I CTNAG 4 cut(s) 191, 873, 1931, 2511
HpySE526I ACGT 4 cut(s) 82, 949, 1091, 2396
Hsp92I GRCGYC 1 cut(s) 2396
HspAI GCGC 1 cut(s) 1497
Kpn2I TCCGGA 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
LguI GCTCTTC 2 cut(s) 354, 684
LmnI GCTCC 5 cut(s) 136, 1382, 1534, 1535, 2196
LweI GCATC 5 cut(s) 79, 1003, 1206, 1645, 2656
MabI ACCWGGT 2 cut(s) 1995, 2083
MaeI CTAG 8 cut(s) 9, 927, 1167, 1349, 1589, 1722, 1922, 2018
MaeII ACGT 4 cut(s) 82, 949, 1091, 2396
MaeIII GTNAC 7 cut(s) 416, 943, 1371, 1432, 1991, 2079, 2239
MalI GATC 6 cut(s) 163, 180, 642, 747, 1134, 1548
MboI GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
MfeI CAATTG 1 cut(s) 448
MflI RGATCY 1 cut(s) 1546
MhlI GDGCHC 4 cut(s) 192, 679, 1943, 2039
MlsI TGGCCA 1 cut(s) 1362
MluNI TGGCCA 1 cut(s) 1362
MlyI GAGTC 3 cut(s) 513, 1005, 2269
MmeI TCCRAC 6 cut(s) 1360, 1717, 1859, 2123, 2156, 2303
Mox20I TGGCCA 1 cut(s) 1362
Mph1103I ATGCAT 1 cut(s) 462
MroI TCCGGA 1 cut(s) 46
MroXI GAANNNNTTC 2 cut(s) 105, 1710
MscI TGGCCA 1 cut(s) 1362
MseI TTAA 9 cut(s) 27, 521, 735, 834, 1023, 1104, 1284, 1541, 2621
MslI CAYNNNNRTG 2 cut(s) 1421, 2655
Msp20I TGGCCA 1 cut(s) 1362
MspA1I CMGCKG 3 cut(s) 872, 1583, 2372
MspI CCGG 4 cut(s) 47, 672, 2452, 2574
MunI CAATTG 1 cut(s) 448
Mva1269I GAATGC 3 cut(s) 460, 2239, 2392
NcoI CCATGG 1 cut(s) 310
NdeI CATATG 2 cut(s) 549, 766
NdeII GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
NlaIV GGNNCC 5 cut(s) 138, 537, 670, 1941, 2192
NmuCI GTSAC 3 cut(s) 1991, 2079, 2239
NsiI ATGCAT 1 cut(s) 462
NspI RCATGY 2 cut(s) 1379, 2306
NspV TTCGAA 1 cut(s) 99
PagI TCATGA 1 cut(s) 1297
PaqCI CACCTGC 2 cut(s) 1810, 2621
PasI CCCWGGG 1 cut(s) 532
PceI AGGCCT 1 cut(s) 42
PciI ACATGT 1 cut(s) 1375
PciSI GCTCTTC 2 cut(s) 354, 684
PcsI WCGNNNNNNNCGW 3 cut(s) 79, 1894, 2158
PctI GAATGC 3 cut(s) 460, 2239, 2392
PdmI GAANNNNTTC 2 cut(s) 105, 1710
PfeI GAWTC 8 cut(s) 572, 814, 931, 1972, 2179, 2318, 2485, 2521
PflFI GACNNNGTC 2 cut(s) 21, 1403
PflMI CCANNNNNTGG 1 cut(s) 158
PfoI TCCNGGA 5 cut(s) 703, 720, 1531, 2193, 2613
PleI GAGTC 3 cut(s) 512, 1004, 2269
PpsI GAGTC 3 cut(s) 512, 1004, 2269
Ppu21I YACGTR 1 cut(s) 1092
PpuMI RGGWCCY 2 cut(s) 707, 724
PscI ACATGT 1 cut(s) 1375
PshBI ATTAAT 1 cut(s) 2621
PsiI TTATAA 1 cut(s) 1947
Psp5II RGGWCCY 2 cut(s) 707, 724
PspEI GGTNACC 2 cut(s) 1991, 2079
PspFI CCCAGC 2 cut(s) 1845, 2109
PspN4I GGNNCC 5 cut(s) 138, 537, 670, 1941, 2192
PspOMI GGGCCC 1 cut(s) 1939
PspPPI RGGWCCY 2 cut(s) 707, 724
PsrI GAACNNNNNNTAC 2 cut(s) 1162, 1194
PstI CTGCAG 2 cut(s) 145, 1669
PstNI CAGNNNCTG 3 cut(s) 470, 1318, 1532
PsuI RGATCY 1 cut(s) 1546
PsyI GACNNNGTC 2 cut(s) 21, 1403
PvuII CAGCTG 1 cut(s) 2372
RsaI GTAC 7 cut(s) 487, 915, 992, 1347, 1555, 1607, 2434
RsaNI GTAC 7 cut(s) 486, 914, 991, 1346, 1554, 1606, 2433
RseI CAYNNNNRTG 2 cut(s) 1421, 2655
SapI GCTCTTC 2 cut(s) 354, 684
SaqAI TTAA 9 cut(s) 27, 521, 735, 834, 1023, 1104, 1284, 1541, 2621
Sau3AI GATC 6 cut(s) 161, 178, 640, 745, 1132, 1546
ScaI AGTACT 1 cut(s) 487
SchI GAGTC 3 cut(s) 513, 1005, 2269
SduI GDGCHC 4 cut(s) 192, 679, 1943, 2039
SexAI ACCWGGT 2 cut(s) 1995, 2083
SfaNI GCATC 5 cut(s) 79, 1003, 1206, 1645, 2656
SfcI CTRYAG 4 cut(s) 141, 214, 1566, 1665
SfuI TTCGAA 1 cut(s) 99
SgrAI CRCCGGYG 1 cut(s) 2573
SinI GGWCC 6 cut(s) 385, 669, 707, 724, 1585, 2454
SmiMI CAYNNNNRTG 2 cut(s) 1421, 2655
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SnaBI TACGTA 1 cut(s) 1092
SpeI ACTAGT 1 cut(s) 1166
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 4 cut(s) 5, 870, 1016, 1583
SspI AATATT 4 cut(s) 618, 984, 1951, 2287
SspMI CTAG 8 cut(s) 9, 927, 1167, 1349, 1589, 1722, 1922, 2018
StuI AGGCCT 1 cut(s) 42
StyI CCWWGG 2 cut(s) 310, 1245
TaaI ACNGT 9 cut(s) 635, 1077, 1255, 1278, 1438, 1777, 1861, 2125, 2245
TaiI ACGT 4 cut(s) 85, 952, 1094, 2399
TaqI TCGA 8 cut(s) 73, 99, 155, 613, 1912, 2483, 2590, 2642
TatI WGTACW 1 cut(s) 485
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 8 cut(s) 572, 814, 931, 1972, 2179, 2318, 2485, 2521
Tru1I TTAA 9 cut(s) 27, 521, 735, 834, 1023, 1104, 1284, 1541, 2621
Tru9I TTAA 9 cut(s) 27, 521, 735, 834, 1023, 1104, 1284, 1541, 2621
TscAI CASTG 3 cut(s) 252, 1147, 2601
TseFI GTSAC 3 cut(s) 1991, 2079, 2239
Tsp45I GTSAC 3 cut(s) 1991, 2079, 2239
TspRI CASTG 3 cut(s) 252, 1147, 2601
Tth111I GACNNNGTC 2 cut(s) 21, 1403
Van91I CCANNNNNTGG 1 cut(s) 158
VpaK11BI GGWCC 6 cut(s) 385, 669, 707, 724, 1585, 2454
VspI ATTAAT 1 cut(s) 2621
XceI RCATGY 2 cut(s) 1379, 2306
XmnI GAANNNNTTC 2 cut(s) 105, 1710
XspI CTAG 8 cut(s) 9, 927, 1167, 1349, 1589, 1722, 1922, 2018
ZraI GACGTC 1 cut(s) 2397
ZrmI AGTACT 1 cut(s) 487
Zsp2I ATGCAT 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.