Rh2BG255100

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001949
Physical Location & Seq
Reverse (-)
26400041 .. 26411343
11303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG255100.1

Sequence Viewer

Length: 3117 bp
ATGTCGGAGACCAAGCGCTATGGTCTTGGAAATCAATTAGATATTCAGCAGATACTTTTAGAAGCTAAACACCGATGGCTACGCCCTGCTGAAATATGTGAAATTCTTCAAAATTATAAAAAATTTCATATATCTTCAGAGCCTGCAAGTACGCCACCTGGCGGATCACTTTTTCTTTTCGATCGGAAGGTGCTGAGGTATTTCAGGAAAGATGGTCATAACTGGAGGAAGAAAAAGGATGGAAAGACAGTGAAGGAGGCCCATGAGAGGCTCAAGGCTGGAAGTGTTGATGTGTTGCACTGCTACTATGCCCATGGAGAAGACAACGAAAATTTCCAAAGACGCAGTTATTGGATGCTTGAAGAGGAGCTCTCGCACATTGTTCTCGTCCACTACCGGGAAGTAAAGGGAAATAGGACAAATTTCAATCATGTTAAGGAGAACGAAGGTGTTCCCTACTCCAATGGAGCTGAAGAAATTGCACGCCAGTCTGAGATGGAGAATTCTGTTTCTTCTAGTTTCAATCCAAGTAGTTACCAAATGCGTTCTCAAGTTACGGACGCGACAAGCTTGAGTAGTGCTCAGGCATCAGAGTTCGAAGATGTGGAATCAGCATTTTATAACCAAGCAAGCTCCCGGTTGCAGCCTATGGCTGAGAAGATTAATTCTGAGTTCTCTGATGCGTACTATCCCGCATTTTCAAATGATTTTCAAGAAAACTTGGCAACCATTCCTGGGGTGGATTTTAGCTCACTCTCTCAAGCATATAAGAGGGAGGATAGTATTGATGCTGGAGATACCTATGAGCCCAGGAAAGATCGTGAATTTGCATCATGGGATGATATGGAAAACAGTGCTACTGGCATTCAATCTTTTCAGCCATCATTTTCAGCAACACATTCAGATACAATGGGAAGCTTTCCTAAGATTGAGACGCTTGGGCATCTTCATACAGACAGTTTTGATAAAAGGCTGGTGTATGGGATTGAAAATAAACCAAAGGTCCAACAAAGTTGGGAGACTTCCGAAGGATCATCAAACTGGCCTGTGGATCAGAGCTTGCAATCACACACAGAATACAATGTCACCACTAAGCTCGATGAAGGAGCTGATACCACTGATTTACTCAAGTCCTTGGGGCCATTCCTTATGGATCCAGACAAGCAAAATGACCTTCAATTTCATCTTTCAAACACAGATACTATTTCAAAGAGAAATGATAGCATTGAGAGAAAAGCTGATCACCCTTCTGCTATTAAGCCTCTGTTGGGATTCGATGATGGTCTAAAGAAGCTCGACAGTTTCAACCGATGGATGAGTAAAGAACTTGAAGATGTGGATGAGCCACAAATGCAGTCCAGTTCTGGAGCCTACTGGGATACTGTTGAAAGTGAGAATGAGGTTGATGAGTCCAGTGTTCCCCTGCAAGTACGCTTGGATAGCTATATGCTGGGGCCTTCTCTTTCTCATGACCAGCTCTTTAGCATTGTTGACTTCTCACCAAACTGGGCTTATGAAAAATCTGAAATCAAGGTTTTGATCACAGGAAAATTTTTGAAAAGTCAACATGTAGAAAGTTGTAAATGGTCATGTATGTTTGGGGAAGTTGAAGTTCCTGCTGAGGTTATAGCACATGGTGTTCTTCGTTGTTATACTCCCATACACAAGGCTGGAAGGGTTCCCTTCTATGTTACATGTTCCAATAGGCTGGCTTGTAGTGAAGTAAGGGAATTTGAATACCGAGTCGCCCAAACTCAAGATGTTGATACTAAGGATTATTACAGTGACTGCTCAAATGAAACACTCAATAAGCGCTTCGAAAAATTATTGTCTTTAAGCTCTACATCTTCAAATTCCGATACAGCAAGTACACTAGAGGATTCTGAGTTGAACAGTAAAATTACTTCTATGCTGAAAAGTGACGATGATGAATGGGACAAGATGCTACAACTTATGTCAGTTGATGAATTTTCCTTGGAAAGAGTACAGGAGCAGTTGCTTCAACAGTTACTTAAAGAGAAGTTGCGTGCATGGCTCTTGCAGAAGCTGGCTGCTGGTGGTAAAGGCCCTAGTGTTCTAGACGAGGGTGGCCAAGGTGTGTTACATTTTGGAGCTGCTCTTGGCTATGACTGGGTCTTATTGCCCACAATAACTGCTGGAGTTAGCGTCAATTTTCGTGATGTGAATGGATGGACGGCACTTCATTGGGCAGCTTTTTGTGGCAGAGAGCGCACAGTCGCTTCTCTCATCGCCCTGGGTGCAGCTCCTGGAGCATTAACAGATCCAACTACCAAATATCCTTCAGGAGAAACACCTGCAGACCTAGCTTCTGAACAAGGACACAAAGGAATTGCTGGATATCTTGCAGAATCTGCATTGAGTGCTCACCTTGAATCTCTTAATTTGGATCTCAAGGACGGAAATTCGACAGAGATTTCAGGAACAAAAGCAGTTTCAAGTTCTAGCAGGGATGGGGAGTTAACGGATGGGCTTTCCCTGAGGGATTCTTTAACTGCTGTCTGTAATGCGACACAGGCTGCTGCTCGTATTCATCAAGTCTTCAGGGTACAATCTTTCCAAAGGAAGCAGTTGAAAGAGTATGATGGTGATAAATTTGGACTTTCCAATGAACGGGCACTATCACTTATTGCAGTCAAGTCAAATAAGGCAGGGAAACGTGATGAGCATGTAGATGCTGCAGCAGTACGAATACAAAATAAGTTCCGTAGTTGGAAGGGTAGAAAAGACTATTTGATAATCCGACAAAGGATTGTCAAAATTCAGGCCCATGTAAGAGGTCACCAGGTGAGGAAAAACTATAGAAAGATAGTCTGGTCCGTAGGAATTGTGGAGAAAATCATCCTGCGTTGGAGAAGAAAGGGAAGTGGGTTGCGCGGGTTCAAGCCAGAACCAATTACTGAGGCCCCCAGCATGCAAGTTTCATCCACAAAGGATGATGATTATGACTTGCTGAAAGAAGGAAGGAAGCAAACAGAGGAAAGGTTGCAAAAGGCACTTGCTAGGGTGAAATCCATGGTTCAGTATCCTGAAGCAAGAGATCAATACCATCGACTGTTAAATGTTGTGACTGAGAGTCAGGAAACAAAGGTAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

1038

Amino Acids

117.2

Weight (kDa)

5.74

Isoelectric Point (pI)

42.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 21 - 134 3.3e-50 CG-1 domain
Ank_2 PF12796 703 - 791 9.1e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 117, 621
AarI CACCTGC 1 cut(s) 2323
Acc36I ACCTGC 1 cut(s) 2323
AccII CGCG 2 cut(s) 563, 2895
AciI CCGC 3 cut(s) 162, 693, 2895
AclWI GGATC 7 cut(s) 172, 1039, 1059, 1148, 1161, 2276, 2415
AcoI YGGCCR 1 cut(s) 2089
AcuI CTGAAG 5 cut(s) 120, 492, 2286, 2545, 3069
AdeI CACNNNGTG 2 cut(s) 1637, 2806
AfaI GTAC 7 cut(s) 151, 686, 1431, 1870, 1986, 2568, 2706
AfeI AGCGCT 2 cut(s) 17, 1814
AfiI CCNNNNNNNGG 6 cut(s) 161, 267, 397, 735, 1268, 2631
AflIII ACRYGT 2 cut(s) 1567, 1694
AhdI GACNNNNNGTC 1 cut(s) 3093
AjnI CCWGG 6 cut(s) 157, 733, 809, 2253, 2266, 2802
Alw21I GWGCWC 3 cut(s) 372, 583, 2386
Alw26I GTCTC 3 cut(s) 2, 926, 1013
AlwI GGATC 7 cut(s) 172, 1039, 1059, 1148, 1161, 2276, 2415
AlwNI CAGNNNCTG 5 cut(s) 143, 1788, 2047, 2267, 2372
Aor51HI AGCGCT 2 cut(s) 17, 1814
AoxI GGCC 8 cut(s) 258, 1043, 1139, 1454, 2065, 2089, 2784, 2922
ApeKI GCWGC 9 cut(s) 643, 2051, 2114, 2210, 2261, 2537, 2540, 2696, 2699
AseI ATTAAT 1 cut(s) 663
Asp700I GAANNNNTTC 2 cut(s) 105, 450
AspLEI GCGC 4 cut(s) 18, 1815, 2232, 2895
AspS9I GGNCC 8 cut(s) 259, 1003, 1139, 1454, 2066, 2785, 2835, 2923
AsuC2I CCSGG 2 cut(s) 398, 637
AsuHPI GGTGA 8 cut(s) 1078, 1235, 1491, 2378, 2618, 2792, 2818, 3037
AsuII TTCGAA 2 cut(s) 597, 1818
AvaII GGWCC 2 cut(s) 1003, 2835
AxyI CCTNAGG 1 cut(s) 2498
BaeGI GKGCMC 1 cut(s) 2638
BalI TGGCCA 1 cut(s) 2091
BamHI GGATCC 1 cut(s) 1153
BanII GRGCYC 2 cut(s) 372, 810
BarI GAAGNNNNNNTAC 2 cut(s) 1888, 1920
BbsI GAAGAC 2 cut(s) 327, 2551
Bbv12I GWGCWC 3 cut(s) 372, 583, 2386
BbvCI CCTCAGC 2 cut(s) 194, 1620
BbvI GCAGC 9 cut(s) 655, 2038, 2101, 2222, 2273, 2524, 2527, 2683, 2711
BceAI ACGGC 1 cut(s) 2211
BciT130I CCWGG 6 cut(s) 159, 735, 811, 2255, 2268, 2804
BciVI GTATCC 2 cut(s) 1372, 3054
BclI TGATCA 2 cut(s) 1240, 1539
BcnI CCSGG 2 cut(s) 398, 637
BcoDI GTCTC 3 cut(s) 2, 926, 1013
BfaI CTAG 7 cut(s) 516, 1874, 2070, 2078, 2324, 2463, 3021
BfmI CTRYAG 3 cut(s) 2316, 2697, 2818
BfoI RGCGCY 2 cut(s) 19, 1816
BfuAI ACCTGC 1 cut(s) 2323
BfuI GTATCC 2 cut(s) 1372, 3054
BisI GCNGC 9 cut(s) 644, 2052, 2115, 2211, 2262, 2538, 2541, 2697, 2700
BlsI GCNGC 9 cut(s) 645, 2053, 2116, 2212, 2263, 2539, 2542, 2698, 2701
Bme1390I CCNGG 8 cut(s) 159, 398, 637, 735, 811, 2255, 2268, 2804
Bme18I GGWCC 2 cut(s) 1003, 2835
BmeRI GACNNNNNGTC 1 cut(s) 3093
BmgT120I GGNCC 8 cut(s) 259, 1003, 1139, 1454, 2066, 2785, 2835, 2923
BmiI GGNNCC 6 cut(s) 1140, 1155, 1369, 1455, 1680, 2925
BmrFI CCNGG 8 cut(s) 159, 398, 637, 735, 811, 2255, 2268, 2804
BmrI ACTGGG 3 cut(s) 1384, 1516, 2140
BmsI GCATC 8 cut(s) 345, 596, 670, 778, 839, 952, 1932, 2683
BmuI ACTGGG 3 cut(s) 1384, 1516, 2140
BpiI GAAGAC 2 cut(s) 327, 2551
BplI GAGNNNNNCTC 4 cut(s) 356, 388, 565, 597
BpmI CTGGAG 5 cut(s) 244, 813, 1386, 2178, 2289
Bpu10I CCTNAGC 3 cut(s) 194, 582, 1620
Bpu14I TTCGAA 2 cut(s) 597, 1818
BpuEI CTTGAG 7 cut(s) 257, 534, 592, 744, 1112, 1740, 2396
BpuMI CCSGG 2 cut(s) 398, 637
BsaI GGTCTC 1 cut(s) 2
BsaJI CCNNGG 9 cut(s) 313, 734, 809, 1134, 1974, 2092, 2253, 2254, 3033
BsaXI ACNNNNNCTCC 2 cut(s) 2151, 2181
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 267, 397, 735, 1268, 2631
Bse1I ACTGG 9 cut(s) 227, 487, 865, 1046, 1359, 1379, 1413, 1511, 2135
Bse21I CCTNAGG 1 cut(s) 2498
BseBI CCWGG 6 cut(s) 159, 735, 811, 2255, 2268, 2804
BseDI CCNNGG 9 cut(s) 313, 734, 809, 1134, 1974, 2092, 2253, 2254, 3033
BseLI CCNNNNNNNGG 6 cut(s) 161, 267, 397, 735, 1268, 2631
BseNI ACTGG 9 cut(s) 227, 487, 865, 1046, 1359, 1379, 1413, 1511, 2135
BseRI GAGGAG 1 cut(s) 380
BseSI GKGCMC 1 cut(s) 2638
BseXI GCAGC 9 cut(s) 655, 2038, 2101, 2222, 2273, 2524, 2527, 2683, 2711
BseYI CCCAGC 2 cut(s) 1450, 2927
BsgI GTGCAG 1 cut(s) 2280
Bsh1236I CGCG 2 cut(s) 563, 2895
Bsh1285I CGRYCG 1 cut(s) 184
BshFI GGCC 8 cut(s) 260, 1045, 1141, 1456, 2067, 2091, 2786, 2924
BsiEI CGRYCG 1 cut(s) 184
BsiHKAI GWGCWC 3 cut(s) 372, 583, 2386
BsiSI CCGG 2 cut(s) 397, 637
BslFI GGGAC 1 cut(s) 1949
BslI CCNNNNNNNGG 6 cut(s) 161, 267, 397, 735, 1268, 2631
BsmAI GTCTC 3 cut(s) 2, 926, 1013
BsmBI CGTCTC 1 cut(s) 926
BsmFI GGGAC 1 cut(s) 1949
BsmI GAATGC 1 cut(s) 864
BsnI GGCC 8 cut(s) 260, 1045, 1141, 1456, 2067, 2091, 2786, 2924
Bso31I GGTCTC 1 cut(s) 2
Bsp119I TTCGAA 2 cut(s) 597, 1818
Bsp1286I GDGCHC 5 cut(s) 372, 583, 810, 2386, 2638
Bsp19I CCATGG 2 cut(s) 313, 3033
BspACI CCGC 3 cut(s) 162, 693, 2895
BspANI GGCC 8 cut(s) 260, 1045, 1141, 1456, 2067, 2091, 2786, 2924
BspFNI CGCG 2 cut(s) 563, 2895
BspHI TCATGA 1 cut(s) 1468
BspLI GGNNCC 6 cut(s) 1140, 1155, 1369, 1455, 1680, 2925
BspMAI CTGCAG 2 cut(s) 2320, 2701
BspMI ACCTGC 1 cut(s) 2323
BspPI GGATC 7 cut(s) 172, 1039, 1059, 1148, 1161, 2276, 2415
BspT104I TTCGAA 2 cut(s) 597, 1818
BspTNI GGTCTC 1 cut(s) 2
BsrI ACTGG 9 cut(s) 227, 487, 865, 1046, 1359, 1379, 1413, 1511, 2135
BssECI CCNNGG 9 cut(s) 313, 734, 809, 1134, 1974, 2092, 2253, 2254, 3033
BssT1I CCWWGG 5 cut(s) 313, 1134, 1974, 2092, 3033
Bst2UI CCWGG 6 cut(s) 159, 735, 811, 2255, 2268, 2804
Bst6I CTCTTC 1 cut(s) 357
BstAPI GCANNNNNTGC 2 cut(s) 2372, 2381
BstBI TTCGAA 2 cut(s) 597, 1818
BstC8I GCNNGC 8 cut(s) 144, 484, 631, 1061, 1710, 2028, 2049, 2933
BstDSI CCRYGG 2 cut(s) 313, 3033
BstEII GGTNACC 1 cut(s) 2798
BstFNI CGCG 2 cut(s) 563, 2895
BstH2I RGCGCY 2 cut(s) 19, 1816
BstHHI GCGC 4 cut(s) 18, 1815, 2232, 2895
BstMAI GTCTC 3 cut(s) 2, 926, 1013
BstMCI CGRYCG 1 cut(s) 184
BstNI CCWGG 6 cut(s) 159, 735, 811, 2255, 2268, 2804
BstNSI RCATGY 4 cut(s) 1571, 1698, 2690, 2935
BstPI GGTNACC 1 cut(s) 2798
BstSCI CCNGG 8 cut(s) 157, 396, 635, 733, 809, 2253, 2266, 2802
BstSFI CTRYAG 3 cut(s) 2316, 2697, 2818
BstSLI GKGCMC 1 cut(s) 2638
BstUI CGCG 2 cut(s) 563, 2895
BstV1I GCAGC 9 cut(s) 655, 2038, 2101, 2222, 2273, 2524, 2527, 2683, 2711
BstV2I GAAGAC 2 cut(s) 327, 2551
BstX2I RGATCY 3 cut(s) 1153, 2281, 2407
BstXI CCANNNNNNTGG 1 cut(s) 1708
BstYI RGATCY 3 cut(s) 1153, 2281, 2407
Bsu36I CCTNAGG 1 cut(s) 2498
BsuI GTATCC 2 cut(s) 1372, 3054
BsuRI GGCC 8 cut(s) 260, 1045, 1141, 1456, 2067, 2091, 2786, 2924
BtgI CCRYGG 2 cut(s) 313, 3033
BtgZI GCGATG 1 cut(s) 2233
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 6 cut(s) 255, 298, 859, 1116, 1420, 1789
BveI ACCTGC 1 cut(s) 2323
Cac8I GCNNGC 8 cut(s) 144, 484, 631, 1061, 1710, 2028, 2049, 2933
CaiI CAGNNNCTG 5 cut(s) 143, 1788, 2047, 2267, 2372
CciI TCATGA 1 cut(s) 1468
CfoI GCGC 4 cut(s) 18, 1815, 2232, 2895
Cfr13I GGNCC 8 cut(s) 259, 1003, 1139, 1454, 2066, 2785, 2835, 2923
CseI GACGC 4 cut(s) 351, 569, 943, 2155
CsiI ACCWGGT 1 cut(s) 2802
Csp6I GTAC 7 cut(s) 150, 685, 1430, 1869, 1985, 2567, 2705
CviQI GTAC 7 cut(s) 150, 685, 1430, 1869, 1985, 2567, 2705
DraIII CACNNNGTG 2 cut(s) 1637, 2806
DriI GACNNNNNGTC 1 cut(s) 3093
EaeI YGGCCR 1 cut(s) 2089
Eam1104I CTCTTC 1 cut(s) 357
Eam1105I GACNNNNNGTC 1 cut(s) 3093
EarI CTCTTC 1 cut(s) 357
EciI GGCGGA 1 cut(s) 177
Ecl136II GAGCTC 1 cut(s) 370
Eco130I CCWWGG 5 cut(s) 313, 1134, 1974, 2092, 3033
Eco24I GRGCYC 2 cut(s) 372, 810
Eco31I GGTCTC 1 cut(s) 2
Eco32I GATATC 1 cut(s) 2360
Eco47I GGWCC 2 cut(s) 1003, 2835
Eco47III AGCGCT 2 cut(s) 17, 1814
Eco53kI GAGCTC 1 cut(s) 370
Eco57I CTGAAG 5 cut(s) 120, 492, 2286, 2545, 3069
Eco81I CCTNAGG 1 cut(s) 2498
Eco91I GGTNACC 1 cut(s) 2798
EcoICRI GAGCTC 1 cut(s) 370
EcoO109I RGGNCCY 3 cut(s) 1454, 2066, 2923
EcoO65I GGTNACC 1 cut(s) 2798
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 6 cut(s) 157, 733, 809, 2253, 2266, 2802
EcoRV GATATC 1 cut(s) 2360
EcoT14I CCWWGG 5 cut(s) 313, 1134, 1974, 2092, 3033
EcoT38I GRGCYC 2 cut(s) 372, 810
ErhI CCWWGG 5 cut(s) 313, 1134, 1974, 2092, 3033
Esp3I CGTCTC 1 cut(s) 926
FaqI GGGAC 1 cut(s) 1949
FauI CCCGC 2 cut(s) 700, 2888
FbaI TGATCA 2 cut(s) 1240, 1539
Fnu4HI GCNGC 9 cut(s) 644, 2052, 2115, 2211, 2262, 2538, 2541, 2697, 2700
FriOI GRGCYC 2 cut(s) 372, 810
Fsp4HI GCNGC 9 cut(s) 644, 2052, 2115, 2211, 2262, 2538, 2541, 2697, 2700
FspBI CTAG 7 cut(s) 516, 1874, 2070, 2078, 2324, 2463, 3021
GlaI GCGC 4 cut(s) 17, 1814, 2231, 2894
GluI GCNGC 9 cut(s) 644, 2052, 2115, 2211, 2262, 2538, 2541, 2697, 2700
GsaI CCCAGC 2 cut(s) 1454, 2931
GsuI CTGGAG 5 cut(s) 244, 813, 1386, 2178, 2289
HaeII RGCGCY 2 cut(s) 19, 1816
HaeIII GGCC 8 cut(s) 260, 1045, 1141, 1456, 2067, 2091, 2786, 2924
HapII CCGG 2 cut(s) 397, 637
HgaI GACGC 4 cut(s) 351, 569, 943, 2155
HhaI GCGC 4 cut(s) 18, 1815, 2232, 2895
Hin6I GCGC 4 cut(s) 16, 1813, 2230, 2893
HinP1I GCGC 4 cut(s) 16, 1813, 2230, 2893
HincII GTYRAC 3 cut(s) 1492, 1565, 2481
HindII GTYRAC 3 cut(s) 1492, 1565, 2481
HindIII AAGCTT 2 cut(s) 568, 916
HinfI GANTC 9 cut(s) 608, 1272, 1409, 1743, 1880, 2369, 2393, 2504, 3094
HpaI GTTAAC 1 cut(s) 2481
HpaII CCGG 2 cut(s) 397, 637
HphI GGTGA 8 cut(s) 1078, 1235, 1491, 2378, 2618, 2792, 2818, 3037
Hpy166II GTNNAC 5 cut(s) 391, 1492, 1565, 1871, 2481
Hpy8I GTNNAC 5 cut(s) 391, 1492, 1565, 1871, 2481
HpyCH4IV ACGT 1 cut(s) 2677
HpySE526I ACGT 1 cut(s) 2677
HspAI GCGC 4 cut(s) 16, 1813, 2230, 2893
Ksp22I TGATCA 2 cut(s) 1240, 1539
KspAI GTTAAC 1 cut(s) 2481
LmnI GCTCC 9 cut(s) 367, 467, 638, 1106, 1367, 1990, 2111, 2269, 2270
Lsp1109I GCAGC 9 cut(s) 655, 2038, 2101, 2222, 2273, 2524, 2527, 2683, 2711
LweI GCATC 8 cut(s) 345, 596, 670, 778, 839, 952, 1932, 2683
MabI ACCWGGT 1 cut(s) 2802
MaeI CTAG 7 cut(s) 516, 1874, 2070, 2078, 2324, 2463, 3021
MaeII ACGT 1 cut(s) 2677
MflI RGATCY 3 cut(s) 1153, 2281, 2407
MhlI GDGCHC 5 cut(s) 372, 583, 810, 2386, 2638
MlsI TGGCCA 1 cut(s) 2091
MluNI TGGCCA 1 cut(s) 2091
MlyI GAGTC 3 cut(s) 1418, 1752, 3103
MmeI TCCRAC 5 cut(s) 1030, 2309, 2711, 2785, 2849
Mox20I TGGCCA 1 cut(s) 2091
MroXI GAANNNNTTC 2 cut(s) 105, 450
MscI TGGCCA 1 cut(s) 2091
MslI CAYNNNNRTG 1 cut(s) 2691
Msp20I TGGCCA 1 cut(s) 2091
MspI CCGG 2 cut(s) 397, 637
MspR9I CCNGG 8 cut(s) 159, 398, 637, 735, 811, 2255, 2268, 2804
Mva1269I GAATGC 1 cut(s) 864
MvaI CCWGG 6 cut(s) 159, 735, 811, 2255, 2268, 2804
MvnI CGCG 2 cut(s) 563, 2895
NciI CCSGG 2 cut(s) 398, 637
NcoI CCATGG 2 cut(s) 313, 3033
NlaIV GGNNCC 6 cut(s) 1140, 1155, 1369, 1455, 1680, 2925
NmuCI GTSAC 5 cut(s) 1084, 1784, 1919, 2798, 3085
NspI RCATGY 4 cut(s) 1571, 1698, 2690, 2935
NspV TTCGAA 2 cut(s) 597, 1818
PaeI GCATGC 1 cut(s) 2935
PagI TCATGA 1 cut(s) 1468
PaqCI CACCTGC 1 cut(s) 2323
PasI CCCWGGG 1 cut(s) 2254
PciI ACATGT 2 cut(s) 1567, 1694
PctI GAATGC 1 cut(s) 864
PdmI GAANNNNTTC 2 cut(s) 105, 450
PfeI GAWTC 6 cut(s) 608, 1272, 1880, 2369, 2393, 2504
PflFI GACNNNGTC 1 cut(s) 2132
PfoI TCCNGGA 1 cut(s) 2266
PkrI GCNGC 9 cut(s) 645, 2053, 2116, 2212, 2263, 2539, 2542, 2698, 2701
Ple19I CGATCG 1 cut(s) 184
PleI GAGTC 3 cut(s) 1417, 1751, 3102
PpsI GAGTC 3 cut(s) 1417, 1751, 3102
PscI ACATGT 2 cut(s) 1567, 1694
PshBI ATTAAT 1 cut(s) 663
PsiI TTATAA 2 cut(s) 117, 621
Psp124BI GAGCTC 1 cut(s) 372
Psp6I CCWGG 6 cut(s) 157, 733, 809, 2253, 2266, 2802
PspEI GGTNACC 1 cut(s) 2798
PspFI CCCAGC 2 cut(s) 1450, 2927
PspGI CCWGG 6 cut(s) 157, 733, 809, 2253, 2266, 2802
PspN4I GGNNCC 6 cut(s) 1140, 1155, 1369, 1455, 1680, 2925
PspPI GGNCC 8 cut(s) 259, 1003, 1139, 1454, 2066, 2785, 2835, 2923
PstI CTGCAG 2 cut(s) 2320, 2701
PstNI CAGNNNCTG 5 cut(s) 143, 1788, 2047, 2267, 2372
PsuI RGATCY 3 cut(s) 1153, 2281, 2407
PsyI GACNNNGTC 1 cut(s) 2132
PvuI CGATCG 1 cut(s) 184
RsaI GTAC 7 cut(s) 151, 686, 1431, 1870, 1986, 2568, 2706
RsaNI GTAC 7 cut(s) 150, 685, 1430, 1869, 1985, 2567, 2705
RseI CAYNNNNRTG 1 cut(s) 2691
SacI GAGCTC 1 cut(s) 372
SatI GCNGC 9 cut(s) 644, 2052, 2115, 2211, 2262, 2538, 2541, 2697, 2700
Sau96I GGNCC 8 cut(s) 259, 1003, 1139, 1454, 2066, 2785, 2835, 2923
SchI GAGTC 3 cut(s) 1418, 1752, 3103
ScrFI CCNGG 8 cut(s) 159, 398, 637, 735, 811, 2255, 2268, 2804
SduI GDGCHC 5 cut(s) 372, 583, 810, 2386, 2638
SexAI ACCWGGT 1 cut(s) 2802
SfaNI GCATC 8 cut(s) 345, 596, 670, 778, 839, 952, 1932, 2683
SfcI CTRYAG 3 cut(s) 2316, 2697, 2818
SfuI TTCGAA 2 cut(s) 597, 1818
SinI GGWCC 2 cut(s) 1003, 2835
SmiMI CAYNNNNRTG 1 cut(s) 2691
SmlI CTYRAG 7 cut(s) 272, 549, 571, 759, 1127, 1755, 2411
SmoI CTYRAG 7 cut(s) 272, 549, 571, 759, 1127, 1755, 2411
SphI GCATGC 1 cut(s) 2935
SsiI CCGC 3 cut(s) 162, 693, 2895
SspMI CTAG 7 cut(s) 516, 1874, 2070, 2078, 2324, 2463, 3021
SstI GAGCTC 1 cut(s) 372
StyD4I CCNGG 8 cut(s) 157, 396, 635, 733, 809, 2253, 2266, 2802
StyI CCWWGG 5 cut(s) 313, 1134, 1974, 2092, 3033
TaiI ACGT 1 cut(s) 2680
TaqI TCGA 8 cut(s) 180, 597, 1098, 1275, 1296, 1818, 2426, 3070
TatI WGTACW 2 cut(s) 1868, 1984
TfiI GAWTC 6 cut(s) 608, 1272, 1880, 2369, 2393, 2504
TscAI CASTG 6 cut(s) 255, 305, 859, 1123, 1420, 1789
TseFI GTSAC 5 cut(s) 1084, 1784, 1919, 2798, 3085
TseI GCWGC 9 cut(s) 643, 2051, 2114, 2210, 2261, 2537, 2540, 2696, 2699
Tsp45I GTSAC 5 cut(s) 1084, 1784, 1919, 2798, 3085
TspGWI ACGGA 5 cut(s) 572, 2433, 2498, 2714, 2827
TspRI CASTG 6 cut(s) 255, 305, 859, 1123, 1420, 1789
Tth111I GACNNNGTC 1 cut(s) 2132
VpaK11BI GGWCC 2 cut(s) 1003, 2835
VspI ATTAAT 1 cut(s) 663
XbaI TCTAGA 1 cut(s) 2077
XceI RCATGY 4 cut(s) 1571, 1698, 2690, 2935
XcmI CCANNNNNNNNNTGG 1 cut(s) 736
XmnI GAANNNNTTC 2 cut(s) 105, 450
XspI CTAG 7 cut(s) 516, 1874, 2070, 2078, 2324, 2463, 3021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.