Rorug02G0186400

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000247
Physical Location & Seq
Forward (+)
17367423 .. 17374904
7482 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0186400.1

Sequence Viewer

Length: 2505 bp
ATGGAGATATCAATGTTCAAAGCACTTCTTAATAAAATCTCCTCTTTTTTCCATTTTTCATCCCATGAGAACATAAATTTGGATCCTGTTCAGAAGTACTACAAAAAAACAGAAGAGATATTAAAATTGTTGAACAATGTACTTGATGCCATTGTTGATTCTGAGATAGCTTCTTATGAAGTGCTTAATAAGCCATTTGAAGAACTGGGTCACTATCTTGATGAATTGAGGGAGCAATTTGAAGACTGGCAACCATTGTTGAGTAAAGTGAATCTTGTTCTGCAAGTTGAATCATGGATATCAAAGATTTCGACTCGTAGCCTGGATGTCTTTCAGCTGCTGAAGACTTCTGAGCAACATCTCCCTGACGAATTGGGTTCAGCATCTCTTGAGCTCTGCATACAGAAACTAAAGCATATGAGCTATGAGCAAATATCAACTGTCATTAAAGATGCTATAAGTGATCTAGTGGAGGGTATTGCGCCTAGCTCAGAGATCCTAGTGAAAATTGCAGAGGGCCTTAGCTTGAGGTCGAATCAGGAGATCCTTATTGAGGCTGTGGCACTCGAAAAGCTGAAGGAGAATGCTGAACAATCTGAAAAGATCGAGGAAGCAGAATATATTGAGCATATGATAGCCCTCGTCACTCGTATGCATGAGCGTCTTATTACGATAAAGCAGTCTGAAAGCTGCAGCGCAGTTCCAATACCTGCTGATTTCTGCTGTCCTCTCTCTCTGGAGCTGATGACAGATCCAGTGATTGTGGCATCAGGACAAACCTATGAGAGGACTTTCATCAAGAATTGGATTGATCTTGGCCTCACAGTTTGTCCTAAGACACGGCAGACTTTAGCTCACACCAATTTGATACCTAATTACACTGTAAAGGCACTGATTGCAAATTGGTGCGAGTCAAACAATGTGAAACTGCCTGATCCCACAAAGTCTATGTGCTTAAATAAGCCAACACACCTTCTTGGACATGCAGAGCCTGGTGCGCCAAAGGACTCGCCTATTCTTCCTCATTCTAGGGTCAATTCATCAATGTCACCTGAGTCAACTCGATCTATGGGTTCCCCCACTAATAAATATATCTCATCTGGTGGATTGCATCGGGAAGGAAGTTCTCCATTCCATCCTCGTTCCGCATCAGAAGGTTCCTTGTCAGTAGCTGGAAATGGGCAGGTTTTGGATATCGCAAGAGTATCATCTACAAGTTCTGAAGACAGATCTGCCGCTATAGACGAAAAGGGTACAGATCTAGTGAGCCAACAGTCTACATCACCATCCAGAAATGAATTTCCTATTACTACTGAAGCCAACCAGTCGTCGCAAAGCCATAATAGAAGTGCCTCAGCATCCGGCATACTTTCTAATACCAATGGTACTCGAGAAAATGCAGTAGATGCCAATGGGTCCTTACAGACGTCAGGCAACCTGTCAGGCTACAGCAGTGATGCTTCTGGGGAGTTTAAATCAGGACCACCAGCTGCTAGCCCTCAACACAGAGAAACTGAGTTGCCAACCAGATTGCTAGAGGCAAGACCTCGAAACCTAATGTGGCGCCGACCTTCTGGGAACTTAATCCCAAGGATAGTTTTGACTCCATCCACTGAAACAAGACCTGACCTTTCTGGACTAGAAGCCCAAGTTCGAACTCTGGTAGAGGACTTGAAAAGCACTTCACTTGATACTCAAAGAGAAGCAACATATGAACTCCGGATGCTTGCAAAGCACAATATGGATAATCGGATTGTTATTGCAAACTGTGGGGCCATTACCTTGTTGGTGGAATTGCTCCGTTCTACAGATTCAAGGGTCCAGGAAAATTCTGTCACAGCACTTCTTAACTTGTCGATCAATGATAACAACAAAACTTTAATTGCTACTGCTAACGCAATTGAACCTCTTATTCATGTCCTTGAGACAGGGAGTGCTGAGGCCAAGGAGAACTCAGCCGCCACTCTGTTTAGCCTTTCGGTGATTGAGGATAACAAGGTTCGTATTGGAAGGTCAGGGGCTATTAGGCCTCTAGTTGATCTATTGGGGAATGGGACTCCTAGAGGGAGGAAAGATGCAGCCACGGCTTTGTTTAATTTGTCAATATTCCATGAGAACAAGAATCGGATTGTTCAAGCTGGTGCTGTAAAGTACTTGGTGGAGTTAATGGACCCAGCTGCTGGAATGGTTGACAAGGCAGTTGCTGTTTTGGCTAATCTTTCTACAATTCCTGAAGGAAGGACGGCAATTGGTCAGGAGGGTGGGATCCCTGTTCTAGTTGAGGTGGTTGAGTTGGGTTCTGCTAGAGGGAAGGAGAATGCAGCTGCTGCTCTTTTACAGCTCTGCACAAATAGTAATAGGTATTGCAGTATGGTGCTCCAAGAAGGAGCTGTCCCGCCGTTAGTCGTATTGTCACAATCTGGCACCCCAAGGGCCAAAGAAAAGGCTCAGACACTACTTAGATTCTTCAGAGACCATAGACATGGTAGTGCCGGTAGAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

834

Amino Acids

91.0

Weight (kDa)

5.77

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUB2_N PF25240 2 - 140 4.1e-57 PUB2-like, N-terminal domain
U-box PF04564 236 - 306 5.1e-22 U-box domain
ARM_PUB PF25598 550 - 826 9.5e-30 Plant U-box protein ARM repeat region
ARM_ARMC5 PF24768 551 - 723 8.3e-10 ARMC5
Arm PF00514 582 - 619 2.9e-09 Armadillo/beta-catenin-like repeat
Arm PF00514 664 - 701 2.2e-06 Armadillo/beta-catenin-like repeat
ARM_ARMC5 PF24768 696 - 817 4.5e-06 ARMC5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000247)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20722 FvH4_6g47643 FvH4_6g47644 FvH4_6g47651 FvH4_6g47660 FvH4_6g47681 FvH4_6g47710 FvH4_6g47721 FvH4_6g47722 FvH4_6g47723 FvH4_6g47731 FvH4_6g47732 FvH4_7g07791
malus_domestica MD01G1174200.v1.1 MD09G1046300.v1.1 MD17G1055400.v1.1
prunus_persica Prupe.3G260500_v2.0.a1 Prupe.3G260600_v2.0.a1 Prupe.3G260700_v2.0.a1 Prupe.3G260800_v2.0.a1 Prupe.3G260900_v2.0.a1 Prupe.3G261100_v2.0.a1 Prupe.3G261100_v2.0.a1 Prupe.3G261100_v2.0.a1
pyrus_communis pycom01g18590 pycom08g18530 pycom09g03820 pycom09g04260 pycom111g03590 pycom111g04940 pycom111g05070 pycom17g05450
rosa_chinensis RchiOBHm_Chr1g0315341 RchiOBHm_Chr2g0166831
rosa_laevigata RLG00000009567 RLG00000009568 RLG00000013046 RLG00000018053 RLG00000018054 RLG00000018055 RLG00000021644 RLG00000021647 RLG00000021648 RLG00000021651 RLG00000021653 RLG00000021655 RLG00000021656 RLG00000021661 RLG00000021668
rosa_multiflora Rmu_co8319251.1_g000001 Rmu_sc0000612.1_g000004 Rmu_sc0000612.1_g000013 Rmu_sc0000612.1_g000022 Rmu_sc0000690.1_g000022 Rmu_sc0000768.1_g000029 Rmu_sc0000820.1_g000021 Rmu_sc0000949.1_g000012 Rmu_sc0001432.1_g000005 Rmu_sc0002322.1_g000037 Rmu_sc0002539.1_g000106 Rmu_sc0002735.1_g000005 Rmu_sc0002966.1_g000004 Rmu_sc0002966.1_g000006 Rmu_sc0002966.1_g000007 Rmu_sc0005344.1_g000001 Rmu_sc0006325.1_g000034 Rmu_sc0006838.1_g000007 Rmu_sc0006838.1_g000019 Rmu_sc0008490.1_g000023 Rmu_sc0008530.1_g000001 Rmu_sc0012743.1_g000005 Rmu_sc0013600.1_g000004 Rmu_sc0016890.1_g000002
rosa_roxburghii Rroxscaffold_1G00012720 Rroxscaffold_1G00013420 Rroxscaffold_2G00083970 Rroxscaffold_2G00084070 Rroxscaffold_2G00084110 Rroxscaffold_2G00084170 Rroxscaffold_2G00084190 Rroxscaffold_2G00084200 Rroxscaffold_2G00084210 Rroxscaffold_2G00084530 Rroxscaffold_2G00084540 Rroxscaffold_2G00129390 Rroxscaffold_2G00129510 Rroxscaffold_4G00327620 Rroxscaffold_4G00328190 Rroxscaffold_5G00340960 Rroxscaffold_5G00340970 Rroxscaffold_7G00187900
rosa_rugosa Rorug02G0186300 Rorug02G0186300 Rorug02G0186400 Rorug02G0525600 Rorug02G0525700 Rorug05G0573300 Rorug06G0132500
rosa_samantha Rh1CG075200 Rh1DG027600 Rh2AG240700 Rh2AG240800 Rh2AG591700 Rh2AG591900 Rh2AG592200 Rh2AG592300 Rh2AG592400 Rh2AG592600 Rh2AG592700 Rh2AG641300 Rh2BG254400 Rh2BG254700 Rh2BG254800 Rh2BG603800 Rh2CG244800 Rh2CG244900 Rh2DG248700 Rh2DG248800 Rh2DG614700 Rh2DG614800 Rh2DG614900 Rh2DG615100 Rh2DG615400 Rh2DG615500 Rh2DG615700 Rh2DG668300 Rh5AG445300 Rh5DG375200 Rh5DG476800 Rh6BG082200 Rh6BG247400 Rh6CG249200
rosa_wichuraiana Rw2G049330 Rw4G005360 Rw5G032950 Rw5G041590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1430
Acc36I ACCTGC 2 cut(s) 718, 1174
AccB1I GGYRCC 2 cut(s) 1563, 2423
AccB7I CCANNNNNTGG 1 cut(s) 2180
AccI GTMKAC 1 cut(s) 1277
AccIII TCCGGA 1 cut(s) 1719
AciI CCGC 4 cut(s) 1146, 1236, 1959, 2396
AclWI GGATC 8 cut(s) 77, 90, 490, 538, 746, 929, 2260, 2273
AcsI RAATTY 3 cut(s) 76, 1298, 1828
AcuI CTGAAG 6 cut(s) 362, 596, 1242, 1335, 2253, 2452
AcyI GRCGYC 2 cut(s) 1427, 1564
AfaI GTAC 5 cut(s) 98, 141, 1255, 1387, 2153
AfiI CCNNNNNNNGG 3 cut(s) 553, 786, 2180
AgsI TTSAA 9 cut(s) 19, 133, 200, 242, 290, 1675, 1815, 1904, 2135
AjnI CCWGG 3 cut(s) 321, 991, 1821
AjuI GAANNNNNNNTTGG 2 cut(s) 62, 94
Alw21I GWGCWC 2 cut(s) 396, 2379
Alw26I GTCTC 2 cut(s) 1919, 2466
AlwI GGATC 8 cut(s) 77, 90, 490, 538, 746, 929, 2260, 2273
AlwNI CAGNNNCTG 6 cut(s) 340, 992, 1172, 2180, 2204, 2327
Ama87I CYCGRG 1 cut(s) 1389
Aor13HI TCCGGA 1 cut(s) 1719
AoxI GGCC 6 cut(s) 517, 817, 1773, 1941, 2027, 2433
ApeKI GCWGC 9 cut(s) 337, 690, 693, 1490, 2078, 2177, 2321, 2324, 2327
ApoI RAATTY 3 cut(s) 76, 1298, 1828
AspLEI GCGC 4 cut(s) 484, 698, 1000, 1566
AspS9I GGNCC 7 cut(s) 517, 1416, 1481, 1773, 1819, 2170, 2433
AsuHPI GGTGA 3 cut(s) 1041, 1275, 1993
AsuII TTCGAA 1 cut(s) 1654
AsuNHI GCTAGC 1 cut(s) 1493
AvaI CYCGRG 1 cut(s) 1389
AvaII GGWCC 4 cut(s) 1416, 1481, 1819, 2170
BamHI GGATCC 2 cut(s) 82, 2265
BanI GGYRCC 2 cut(s) 1563, 2423
BanII GRGCYC 1 cut(s) 396
BbsI GAAGAC 3 cut(s) 249, 350, 1230
Bbv12I GWGCWC 2 cut(s) 396, 2379
BbvCI CCTCAGC 2 cut(s) 1354, 1938
BbvI GCAGC 9 cut(s) 324, 677, 705, 1477, 2090, 2164, 2311, 2314, 2333
BccI CCATC 3 cut(s) 1143, 1294, 1615
BceAI ACGGC 4 cut(s) 857, 2100, 2259, 2383
BciT130I CCWGG 3 cut(s) 323, 993, 1823
BcoDI GTCTC 2 cut(s) 1919, 2466
BfmI CTRYAG 4 cut(s) 691, 1239, 1447, 1806
BfoI RGCGCY 1 cut(s) 1567
BfuAI ACCTGC 2 cut(s) 718, 1174
BglII AGATCT 2 cut(s) 1229, 1258
BmcAI AGTACT 2 cut(s) 98, 2153
Bme1390I CCNGG 3 cut(s) 323, 993, 1823
Bme18I GGWCC 4 cut(s) 1416, 1481, 1819, 2170
BmeT110I CYCGRG 1 cut(s) 1389
BmgT120I GGNCC 7 cut(s) 517, 1416, 1481, 1773, 1819, 2170, 2433
BmrFI CCNGG 3 cut(s) 323, 993, 1823
BmrI ACTGGG 1 cut(s) 215
BmtI GCTAGC 1 cut(s) 1497
BmuI ACTGGG 1 cut(s) 215
BpiI GAAGAC 3 cut(s) 249, 350, 1230
BpmI CTGGAG 1 cut(s) 758
Bpu10I CCTNAGC 3 cut(s) 521, 1354, 1938
Bpu14I TTCGAA 1 cut(s) 1654
BpuEI CTTGAG 3 cut(s) 410, 547, 1943
BsaHI GRCGYC 2 cut(s) 1427, 1564
BsaI GGTCTC 1 cut(s) 2466
BsaJI CCNNGG 4 cut(s) 1589, 1944, 2082, 2429
BsaWI WCCGGW 1 cut(s) 1719
Bsc4I CCNNNNNNNGG 3 cut(s) 553, 786, 2180
Bse118I RCCGGY 1 cut(s) 2492
Bse1I ACTGG 4 cut(s) 210, 251, 755, 1324
BseAI TCCGGA 1 cut(s) 1719
BseBI CCWGG 3 cut(s) 323, 993, 1823
BseDI CCNNGG 4 cut(s) 1589, 1944, 2082, 2429
BseGI GGATG 7 cut(s) 59, 331, 1135, 1286, 1358, 1607, 1728
BseLI CCNNNNNNNGG 3 cut(s) 553, 786, 2180
BseMII CTCAG 9 cut(s) 153, 342, 504, 1044, 1368, 1506, 1929, 1968, 2462
BseNI ACTGG 4 cut(s) 210, 251, 755, 1324
BseRI GAGGAG 1 cut(s) 31
BseXI GCAGC 9 cut(s) 324, 677, 705, 1477, 2090, 2164, 2311, 2314, 2333
BseYI CCCAGC 1 cut(s) 2173
BsgI GTGCAG 1 cut(s) 2329
BshFI GGCC 6 cut(s) 519, 819, 1775, 1943, 2029, 2435
BshNI GGYRCC 2 cut(s) 1563, 2423
BsiHKAI GWGCWC 2 cut(s) 396, 2379
BsiHKCI CYCGRG 1 cut(s) 1389
BsiSI CCGG 3 cut(s) 1362, 1720, 2493
BslFI GGGAC 2 cut(s) 2068, 2378
BslI CCNNNNNNNGG 3 cut(s) 553, 786, 2180
BsmAI GTCTC 2 cut(s) 1919, 2466
BsmFI GGGAC 2 cut(s) 2068, 2378
BsmI GAATGC 2 cut(s) 589, 2323
BsnI GGCC 6 cut(s) 519, 819, 1775, 1943, 2029, 2435
Bso31I GGTCTC 1 cut(s) 2466
BsoBI CYCGRG 1 cut(s) 1389
Bsp119I TTCGAA 1 cut(s) 1654
Bsp1286I GDGCHC 2 cut(s) 396, 2379
Bsp13I TCCGGA 1 cut(s) 1719
BspACI CCGC 4 cut(s) 1146, 1236, 1959, 2396
BspANI GGCC 6 cut(s) 519, 819, 1775, 1943, 2029, 2435
BspCNI CTCAG 9 cut(s) 154, 343, 503, 1045, 1367, 1507, 1930, 1967, 2461
BspEI TCCGGA 1 cut(s) 1719
BspMAI CTGCAG 1 cut(s) 695
BspMI ACCTGC 2 cut(s) 718, 1174
BspOI GCTAGC 1 cut(s) 1497
BspPI GGATC 8 cut(s) 77, 90, 490, 538, 746, 929, 2260, 2273
BspT104I TTCGAA 1 cut(s) 1654
BspT107I GGYRCC 2 cut(s) 1563, 2423
BspTNI GGTCTC 1 cut(s) 2466
BsrFI RCCGGY 1 cut(s) 2492
BsrI ACTGG 4 cut(s) 210, 251, 755, 1324
BssAI RCCGGY 1 cut(s) 2492
BssECI CCNNGG 4 cut(s) 1589, 1944, 2082, 2429
BssNI GRCGYC 2 cut(s) 1427, 1564
BssT1I CCWWGG 3 cut(s) 1589, 1944, 2429
Bst2UI CCWGG 3 cut(s) 323, 993, 1823
Bst4CI ACNGT 5 cut(s) 442, 826, 883, 1275, 1769
Bst6I CTCTTC 1 cut(s) 108
BstACI GRCGYC 2 cut(s) 1427, 1564
BstAPI GCANNNNNTGC 3 cut(s) 896, 1406, 2327
BstBI TTCGAA 1 cut(s) 1654
BstC8I GCNNGC 2 cut(s) 1495, 1728
BstDSI CCRYGG 1 cut(s) 2082
BstENI CCTNNNNNAGG 2 cut(s) 551, 784
BstF5I GGATG 7 cut(s) 59, 331, 1135, 1286, 1358, 1607, 1728
BstH2I RGCGCY 1 cut(s) 1567
BstHHI GCGC 4 cut(s) 484, 698, 1000, 1566
BstMAI GTCTC 2 cut(s) 1919, 2466
BstMWI GCNNNNNNNGC 8 cut(s) 190, 896, 997, 1406, 1732, 2084, 2210, 2327
BstNI CCWGG 3 cut(s) 323, 993, 1823
BstNSI RCATGY 1 cut(s) 986
BstSCI CCNGG 3 cut(s) 321, 991, 1821
BstSFI CTRYAG 4 cut(s) 691, 1239, 1447, 1806
BstV1I GCAGC 9 cut(s) 324, 677, 705, 1477, 2090, 2164, 2311, 2314, 2333
BstV2I GAAGAC 3 cut(s) 249, 350, 1230
BstX2I RGATCY 7 cut(s) 82, 495, 543, 751, 1229, 1258, 2265
BstXI CCANNNNNNTGG 1 cut(s) 2483
BstYI RGATCY 7 cut(s) 82, 495, 543, 751, 1229, 1258, 2265
BsuRI GGCC 6 cut(s) 519, 819, 1775, 1943, 2029, 2435
BtgI CCRYGG 1 cut(s) 2082
BtsCI GGATG 7 cut(s) 59, 331, 1135, 1286, 1358, 1607, 1728
BtsI GCAGTG 1 cut(s) 1459
BtsIMutI CAGTG 5 cut(s) 762, 879, 890, 1459, 1611
BveI ACCTGC 2 cut(s) 718, 1174
Cac8I GCNNGC 2 cut(s) 1495, 1728
CaiI CAGNNNCTG 6 cut(s) 340, 992, 1172, 2180, 2204, 2327
CfoI GCGC 4 cut(s) 484, 698, 1000, 1566
Cfr10I RCCGGY 1 cut(s) 2492
Cfr13I GGNCC 7 cut(s) 517, 1416, 1481, 1773, 1819, 2170, 2433
CseI GACGC 1 cut(s) 650
Csp6I GTAC 5 cut(s) 97, 140, 1254, 1386, 2152
CviAII CATG 7 cut(s) 65, 294, 656, 983, 1916, 2111, 2483
CviQI GTAC 5 cut(s) 97, 140, 1254, 1386, 2152
DinI GGCGCC 1 cut(s) 1565
DraI TTTAAA 1 cut(s) 1474
Eam1104I CTCTTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 108
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 3 cut(s) 1589, 1944, 2429
Eco147I AGGCCT 1 cut(s) 2029
Eco24I GRGCYC 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 2466
Eco32I GATATC 3 cut(s) 9, 300, 1195
Eco47I GGWCC 4 cut(s) 1416, 1481, 1819, 2170
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 6 cut(s) 362, 596, 1242, 1335, 2253, 2452
Eco88I CYCGRG 1 cut(s) 1389
EcoICRI GAGCTC 1 cut(s) 394
EcoNI CCTNNNNNAGG 2 cut(s) 551, 784
EcoO109I RGGNCCY 2 cut(s) 517, 1416
EcoRII CCWGG 3 cut(s) 321, 991, 1821
EcoRV GATATC 3 cut(s) 9, 300, 1195
EcoT14I CCWWGG 3 cut(s) 1589, 1944, 2429
EcoT22I ATGCAT 1 cut(s) 657
EcoT38I GRGCYC 1 cut(s) 396
EgeI GGCGCC 1 cut(s) 1565
EheI GGCGCC 1 cut(s) 1565
ErhI CCWWGG 3 cut(s) 1589, 1944, 2429
FaeI CATG 7 cut(s) 68, 297, 659, 986, 1919, 2114, 2486
FalI AAGNNNNNCTT 4 cut(s) 12, 44, 258, 290
FaqI GGGAC 2 cut(s) 2068, 2378
FatI CATG 7 cut(s) 64, 293, 655, 982, 1915, 2110, 2482
FauI CCCGC 1 cut(s) 2403
FauNDI CATATG 3 cut(s) 417, 630, 1711
FblI GTMKAC 1 cut(s) 1277
FokI GGATG 7 cut(s) 46, 338, 1122, 1273, 1345, 1594, 1735
FriOI GRGCYC 1 cut(s) 396
GlaI GCGC 4 cut(s) 483, 697, 999, 1565
GsaI CCCAGC 1 cut(s) 2177
GsuI CTGGAG 1 cut(s) 758
HaeII RGCGCY 1 cut(s) 1567
HaeIII GGCC 6 cut(s) 519, 819, 1775, 1943, 2029, 2435
HapII CCGG 3 cut(s) 1362, 1720, 2493
HgaI GACGC 1 cut(s) 650
HhaI GCGC 4 cut(s) 484, 698, 1000, 1566
Hin1I GRCGYC 2 cut(s) 1427, 1564
Hin1II CATG 7 cut(s) 68, 297, 659, 986, 1919, 2114, 2486
Hin6I GCGC 4 cut(s) 482, 696, 998, 1564
HinP1I GCGC 4 cut(s) 482, 696, 998, 1564
HincII GTYRAC 2 cut(s) 1059, 2191
HindII GTYRAC 2 cut(s) 1059, 2191
HpaII CCGG 3 cut(s) 1362, 1720, 2493
HphI GGTGA 3 cut(s) 1041, 1275, 1993
Hpy166II GTNNAC 3 cut(s) 1059, 1278, 2191
Hpy8I GTNNAC 3 cut(s) 1059, 1278, 2191
Hpy99I CGWCG 1 cut(s) 1333
HpyCH4III ACNGT 5 cut(s) 442, 826, 883, 1275, 1769
HpyCH4IV ACGT 1 cut(s) 1427
HpyF10VI GCNNNNNNNGC 8 cut(s) 190, 896, 997, 1406, 1732, 2084, 2210, 2327
HpySE526I ACGT 1 cut(s) 1427
Hsp92I GRCGYC 2 cut(s) 1427, 1564
Hsp92II CATG 7 cut(s) 68, 297, 659, 986, 1919, 2114, 2486
HspAI GCGC 4 cut(s) 482, 696, 998, 1564
KasI GGCGCC 1 cut(s) 1563
Kpn2I TCCGGA 1 cut(s) 1719
LmnI GCTCC 5 cut(s) 232, 739, 1803, 2382, 2387
Lsp1109I GCAGC 9 cut(s) 324, 677, 705, 1477, 2090, 2164, 2311, 2314, 2333
MaeII ACGT 1 cut(s) 1427
MaeIII GTNAC 5 cut(s) 209, 643, 1047, 1834, 2412
MboII GAAGA 7 cut(s) 125, 212, 254, 355, 1010, 1235, 2458
MfeI CAATTG 2 cut(s) 1899, 2247
MflI RGATCY 7 cut(s) 82, 495, 543, 751, 1229, 1258, 2265
MhlI GDGCHC 2 cut(s) 396, 2379
Mly113I GGCGCC 1 cut(s) 1564
MlyI GAGTC 6 cut(s) 307, 920, 1001, 1064, 1597, 2050
Mph1103I ATGCAT 1 cut(s) 657
MroI TCCGGA 1 cut(s) 1719
MslI CAYNNNNRTG 3 cut(s) 650, 2481, 2487
MspA1I CMGCKG 4 cut(s) 337, 1490, 2177, 2324
MspI CCGG 3 cut(s) 1362, 1720, 2493
MspR9I CCNGG 3 cut(s) 323, 993, 1823
MunI CAATTG 2 cut(s) 1899, 2247
Mva1269I GAATGC 2 cut(s) 589, 2323
MvaI CCWGG 3 cut(s) 323, 993, 1823
MwoI GCNNNNNNNGC 8 cut(s) 190, 896, 997, 1406, 1732, 2084, 2210, 2327
NarI GGCGCC 1 cut(s) 1564
NdeI CATATG 3 cut(s) 417, 630, 1711
NheI GCTAGC 1 cut(s) 1493
NlaIII CATG 7 cut(s) 68, 297, 659, 986, 1919, 2114, 2486
NmuCI GTSAC 5 cut(s) 209, 643, 1047, 1834, 2412
NsiI ATGCAT 1 cut(s) 657
NspI RCATGY 1 cut(s) 986
NspV TTCGAA 1 cut(s) 1654
PaeR7I CTCGAG 1 cut(s) 1389
PceI AGGCCT 1 cut(s) 2029
PctI GAATGC 2 cut(s) 589, 2323
PfeI GAWTC 7 cut(s) 158, 271, 290, 535, 1811, 2122, 2463
PflMI CCANNNNNTGG 1 cut(s) 2180
PfoI TCCNGGA 1 cut(s) 1821
PleI GAGTC 6 cut(s) 307, 919, 1001, 1063, 1597, 2050
PluTI GGCGCC 1 cut(s) 1567
PpsI GAGTC 6 cut(s) 307, 919, 1001, 1063, 1597, 2050
PpuMI RGGWCCY 1 cut(s) 1416
Psp124BI GAGCTC 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 1416
Psp6I CCWGG 3 cut(s) 321, 991, 1821
PspFI CCCAGC 1 cut(s) 2173
PspGI CCWGG 3 cut(s) 321, 991, 1821
PspPI GGNCC 7 cut(s) 517, 1416, 1481, 1773, 1819, 2170, 2433
PspPPI RGGWCCY 1 cut(s) 1416
PstI CTGCAG 1 cut(s) 695
PstNI CAGNNNCTG 6 cut(s) 340, 992, 1172, 2180, 2204, 2327
PsuI RGATCY 7 cut(s) 82, 495, 543, 751, 1229, 1258, 2265
PvuII CAGCTG 4 cut(s) 337, 1490, 2177, 2324
RsaI GTAC 5 cut(s) 98, 141, 1255, 1387, 2153
RsaNI GTAC 5 cut(s) 97, 140, 1254, 1386, 2152
RseI CAYNNNNRTG 3 cut(s) 650, 2481, 2487
SacI GAGCTC 1 cut(s) 396
Sau96I GGNCC 7 cut(s) 517, 1416, 1481, 1773, 1819, 2170, 2433
ScaI AGTACT 2 cut(s) 98, 2153
SchI GAGTC 6 cut(s) 307, 920, 1001, 1064, 1597, 2050
ScrFI CCNGG 3 cut(s) 323, 993, 1823
SduI GDGCHC 2 cut(s) 396, 2379
SfcI CTRYAG 4 cut(s) 691, 1239, 1447, 1806
SfoI GGCGCC 1 cut(s) 1565
Sfr274I CTCGAG 1 cut(s) 1389
SfuI TTCGAA 1 cut(s) 1654
SinI GGWCC 4 cut(s) 1416, 1481, 1819, 2170
SlaI CTCGAG 1 cut(s) 1389
SmiMI CAYNNNNRTG 3 cut(s) 650, 2481, 2487
SmlI CTYRAG 4 cut(s) 389, 526, 1389, 1922
SmoI CTYRAG 4 cut(s) 389, 526, 1389, 1922
SseBI AGGCCT 1 cut(s) 2029
SsiI CCGC 4 cut(s) 1146, 1236, 1959, 2396
SspDI GGCGCC 1 cut(s) 1563
SspI AATATT 1 cut(s) 2106
SstI GAGCTC 1 cut(s) 396
StuI AGGCCT 1 cut(s) 2029
StyD4I CCNGG 3 cut(s) 321, 991, 1821
StyI CCWWGG 3 cut(s) 1589, 1944, 2429
TaaI ACNGT 5 cut(s) 442, 826, 883, 1275, 1769
TaiI ACGT 1 cut(s) 1430
TaqI TCGA 9 cut(s) 311, 533, 567, 606, 1063, 1390, 1549, 1654, 1856
TatI WGTACW 3 cut(s) 96, 139, 2151
TauI GCSGC 2 cut(s) 1238, 1961
TfiI GAWTC 7 cut(s) 158, 271, 290, 535, 1811, 2122, 2463
TscAI CASTG 5 cut(s) 762, 886, 897, 1459, 1618
TseFI GTSAC 5 cut(s) 209, 643, 1047, 1834, 2412
TseI GCWGC 9 cut(s) 337, 690, 693, 1490, 2078, 2177, 2321, 2324, 2327
Tsp45I GTSAC 5 cut(s) 209, 643, 1047, 1834, 2412
TspDTI ATGAA 8 cut(s) 48, 192, 237, 784, 1029, 1311, 1728, 1904
TspGWI ACGGA 1 cut(s) 1790
TspRI CASTG 5 cut(s) 762, 886, 897, 1459, 1618
Van91I CCANNNNNTGG 1 cut(s) 2180
VpaK11BI GGWCC 4 cut(s) 1416, 1481, 1819, 2170
XagI CCTNNNNNAGG 2 cut(s) 551, 784
XapI RAATTY 3 cut(s) 76, 1298, 1828
XceI RCATGY 1 cut(s) 986
XcmI CCANNNNNNNNNTGG 1 cut(s) 1783
XhoI CTCGAG 1 cut(s) 1389
XmiI GTMKAC 1 cut(s) 1277
ZraI GACGTC 1 cut(s) 1428
ZrmI AGTACT 2 cut(s) 98, 2153
Zsp2I ATGCAT 1 cut(s) 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.