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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 53, 536 |
| AclI | AACGTT | 1 cut(s) | 12 |
| AclWI | GGATC | 1 cut(s) | 12 |
| AcoI | YGGCCR | 1 cut(s) | 108 |
| AcsI | RAATTY | 1 cut(s) | 275 |
| AcuI | CTGAAG | 1 cut(s) | 155 |
| AdeI | CACNNNGTG | 1 cut(s) | 475 |
| AfaI | GTAC | 4 cut(s) | 140, 416, 492, 522 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 535 |
| AgsI | TTSAA | 1 cut(s) | 442 |
| AlwI | GGATC | 1 cut(s) | 12 |
| AoxI | GGCC | 1 cut(s) | 108 |
| ApoI | RAATTY | 1 cut(s) | 275 |
| AseI | ATTAAT | 1 cut(s) | 483 |
| AsuHPI | GGTGA | 1 cut(s) | 160 |
| BalI | TGGCCA | 1 cut(s) | 110 |
| BbsI | GAAGAC | 1 cut(s) | 430 |
| BfaI | CTAG | 1 cut(s) | 125 |
| BmsI | GCATC | 2 cut(s) | 164, 523 |
| BpiI | GAAGAC | 1 cut(s) | 430 |
| BsaJI | CCNNGG | 1 cut(s) | 44 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 142, 172 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 535 |
| Bse1I | ACTGG | 1 cut(s) | 101 |
| BseDI | CCNNGG | 1 cut(s) | 44 |
| BseGI | GGATG | 2 cut(s) | 195, 400 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 535 |
| BseMII | CTCAG | 1 cut(s) | 41 |
| BseNI | ACTGG | 1 cut(s) | 101 |
| BshFI | GGCC | 1 cut(s) | 110 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 535 |
| BsnI | GGCC | 1 cut(s) | 110 |
| Bsp1407I | TGTACA | 2 cut(s) | 490, 520 |
| Bsp143I | GATC | 1 cut(s) | 4 |
| BspACI | CCGC | 2 cut(s) | 53, 536 |
| BspANI | GGCC | 1 cut(s) | 110 |
| BspCNI | CTCAG | 1 cut(s) | 40 |
| BspPI | GGATC | 1 cut(s) | 12 |
| BsrGI | TGTACA | 2 cut(s) | 490, 520 |
| BsrI | ACTGG | 1 cut(s) | 101 |
| BssECI | CCNNGG | 1 cut(s) | 44 |
| BssMI | GATC | 1 cut(s) | 4 |
| BssT1I | CCWWGG | 1 cut(s) | 44 |
| Bst4CI | ACNGT | 2 cut(s) | 232, 467 |
| BstAUI | TGTACA | 2 cut(s) | 490, 520 |
| BstC8I | GCNNGC | 3 cut(s) | 51, 122, 312 |
| BstDEI | CTNAG | 2 cut(s) | 27, 405 |
| BstF5I | GGATG | 2 cut(s) | 195, 400 |
| BstKTI | GATC | 1 cut(s) | 7 |
| BstMBI | GATC | 1 cut(s) | 4 |
| BstV2I | GAAGAC | 1 cut(s) | 430 |
| BstX2I | RGATCY | 1 cut(s) | 4 |
| BstYI | RGATCY | 1 cut(s) | 4 |
| BsuRI | GGCC | 1 cut(s) | 110 |
| BtsCI | GGATG | 2 cut(s) | 195, 400 |
| Cac8I | GCNNGC | 3 cut(s) | 51, 122, 312 |
| Csp6I | GTAC | 4 cut(s) | 139, 415, 491, 521 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 49, 84 |
| CviAII | CATG | 2 cut(s) | 106, 251 |
| CviJI | RGCY | 4 cut(s) | 110, 120, 124, 248 |
| CviKI_1 | RGCY | 4 cut(s) | 110, 120, 124, 248 |
| CviQI | GTAC | 4 cut(s) | 139, 415, 491, 521 |
| DdeI | CTNAG | 2 cut(s) | 27, 405 |
| DpnI | GATC | 1 cut(s) | 6 |
| DpnII | GATC | 1 cut(s) | 4 |
| DraIII | CACNNNGTG | 1 cut(s) | 475 |
| EaeI | YGGCCR | 1 cut(s) | 108 |
| Eco130I | CCWWGG | 1 cut(s) | 44 |
| Eco57I | CTGAAG | 1 cut(s) | 155 |
| EcoRI | GAATTC | 1 cut(s) | 275 |
| EcoT14I | CCWWGG | 1 cut(s) | 44 |
| ErhI | CCWWGG | 1 cut(s) | 44 |
| FaeI | CATG | 2 cut(s) | 109, 254 |
| FaiI | YATR | 5 cut(s) | 107, 177, 197, 252, 513 |
| FatI | CATG | 2 cut(s) | 105, 250 |
| FauI | CCCGC | 2 cut(s) | 46, 529 |
| FokI | GGATG | 2 cut(s) | 202, 407 |
| FspBI | CTAG | 1 cut(s) | 125 |
| HaeIII | GGCC | 1 cut(s) | 110 |
| Hin1II | CATG | 2 cut(s) | 109, 254 |
| HinfI | GANTC | 1 cut(s) | 31 |
| HphI | GGTGA | 1 cut(s) | 160 |
| Hpy166II | GTNNAC | 4 cut(s) | 141, 214, 375, 491 |
| Hpy188I | TCNGA | 4 cut(s) | 30, 189, 265, 305 |
| Hpy188III | TCNNGA | 2 cut(s) | 226, 344 |
| Hpy8I | GTNNAC | 4 cut(s) | 141, 214, 375, 491 |
| HpyCH4III | ACNGT | 2 cut(s) | 232, 467 |
| HpyCH4IV | ACGT | 1 cut(s) | 12 |
| HpyCH4V | TGCA | 4 cut(s) | 82, 155, 401, 425 |
| HpyF3I | CTNAG | 2 cut(s) | 27, 405 |
| HpySE526I | ACGT | 1 cut(s) | 12 |
| Hsp92II | CATG | 2 cut(s) | 109, 254 |
| Kzo9I | GATC | 1 cut(s) | 4 |
| LmnI | GCTCC | 1 cut(s) | 259 |
| LpnPI | CCDG | 3 cut(s) | 51, 106, 114 |
| LweI | GCATC | 2 cut(s) | 164, 523 |
| MaeI | CTAG | 1 cut(s) | 125 |
| MaeII | ACGT | 1 cut(s) | 12 |
| MaeIII | GTNAC | 4 cut(s) | 56, 166, 178, 467 |
| MalI | GATC | 1 cut(s) | 6 |
| MboI | GATC | 1 cut(s) | 4 |
| MboII | GAAGA | 2 cut(s) | 286, 430 |
| MflI | RGATCY | 1 cut(s) | 4 |
| MlsI | TGGCCA | 1 cut(s) | 110 |
| MluCI | AATT | 4 cut(s) | 61, 259, 275, 494 |
| MluNI | TGGCCA | 1 cut(s) | 110 |
| MmeI | TCCRAC | 2 cut(s) | 154, 523 |
| MnlI | CCTC | 5 cut(s) | 36, 325, 347, 374, 384 |
| Mox20I | TGGCCA | 1 cut(s) | 110 |
| MscI | TGGCCA | 1 cut(s) | 110 |
| MseI | TTAA | 4 cut(s) | 9, 21, 462, 483 |
| MslI | CAYNNNNRTG | 1 cut(s) | 188 |
| Msp20I | TGGCCA | 1 cut(s) | 110 |
| NdeII | GATC | 1 cut(s) | 4 |
| NlaIII | CATG | 2 cut(s) | 109, 254 |
| NmuCI | GTSAC | 3 cut(s) | 56, 166, 467 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 314 |
| PfeI | GAWTC | 1 cut(s) | 31 |
| PshBI | ATTAAT | 1 cut(s) | 483 |
| Psp1406I | AACGTT | 1 cut(s) | 12 |
| PsuI | RGATCY | 1 cut(s) | 4 |
| RsaI | GTAC | 4 cut(s) | 140, 416, 492, 522 |
| RsaNI | GTAC | 4 cut(s) | 139, 415, 491, 521 |
| RseI | CAYNNNNRTG | 1 cut(s) | 188 |
| SaqAI | TTAA | 4 cut(s) | 9, 21, 462, 483 |
| Sau3AI | GATC | 1 cut(s) | 4 |
| SetI | ASST | 1 cut(s) | 15 |
| SfaNI | GCATC | 2 cut(s) | 164, 523 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 188 |
| Sse9I | AATT | 4 cut(s) | 61, 259, 275, 494 |
| SsiI | CCGC | 2 cut(s) | 53, 536 |
| SspI | AATATT | 1 cut(s) | 78 |
| SspMI | CTAG | 1 cut(s) | 125 |
| StyI | CCWWGG | 1 cut(s) | 44 |
| TaaI | ACNGT | 2 cut(s) | 232, 467 |
| TaiI | ACGT | 1 cut(s) | 15 |
| TaqI | TCGA | 1 cut(s) | 343 |
| TasI | AATT | 4 cut(s) | 61, 259, 275, 494 |
| TatI | WGTACW | 3 cut(s) | 138, 490, 520 |
| TfiI | GAWTC | 1 cut(s) | 31 |
| Tru1I | TTAA | 4 cut(s) | 9, 21, 462, 483 |
| Tru9I | TTAA | 4 cut(s) | 9, 21, 462, 483 |
| TseFI | GTSAC | 3 cut(s) | 56, 166, 467 |
| Tsp45I | GTSAC | 3 cut(s) | 56, 166, 467 |
| TspDTI | ATGAA | 1 cut(s) | 268 |
| VspI | ATTAAT | 1 cut(s) | 483 |
| XapI | RAATTY | 1 cut(s) | 275 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 265 |
| XspI | CTAG | 1 cut(s) | 125 |