MD01G1174200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0000247
Physical Location & Seq
Forward (+)
27546441 .. 27547124
684 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1174200.v1.1.491

Sequence Viewer

Length: 684 bp
ATGTTCTTTAGTTGGCTAGCAAATGTGCTTGCGTTGTGGGCATATATCCTCTATGTAAATTTCACATCCATTATGTATACCATTCACAGCAGCAGCACTAGGGTGGAGGGTGGTCAGTACAATTCTCTACGGCCAGAACACAAATGTATATTCTTTCTCCTTACCATGCTAGGAGTCCTCCTCCAGGTGAAGCATGCGACCGGAGATATGCCGTCTCCATTTGTTACAGACCACAACGCTATTGTGATGTTGATTGCTGATTTATTCACTTACGCGGGGTCATTGGCGACTGTCAATATATTCCAAGCTTCCAACAATACAGAGATATATGAGTTGGTGAACAACCTCAGCCTCTTGTGCGGAACTCTTGCATTGATTCTAATGATGCTCATCCTCATTCCAAATTTTGGTTGGTTTACTCTCGCATGTTGGGTCATATATTTTGTGATAATTGTGACCAAGTCCTACCAAACCCTAAAAAGACTAGGTAATGCAGCTGTTCGTTATGCCCGTCATAAAATGAAGGCAGGTGTTCGTCATGTCTGTAACAAAATGAAGGCAGGTGTTCGTTATGTCTGTGACAGAATGAAGGCAGCAATTCGTTATGTCCGTGACAAAATGAAGGAGCTGATGATCATATCAAAGGGCACCGAAGAACCCCAAAACCAGAACGGGATGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

228

Amino Acids

25.84

Weight (kDa)

9.47

Isoelectric Point (pI)

41.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000247)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20722 FvH4_6g47643 FvH4_6g47644 FvH4_6g47651 FvH4_6g47660 FvH4_6g47681 FvH4_6g47710 FvH4_6g47721 FvH4_6g47722 FvH4_6g47723 FvH4_6g47731 FvH4_6g47732 FvH4_7g07791
malus_domestica MD01G1174200.v1.1 MD09G1046300.v1.1 MD17G1055400.v1.1
prunus_persica Prupe.3G260500_v2.0.a1 Prupe.3G260600_v2.0.a1 Prupe.3G260700_v2.0.a1 Prupe.3G260800_v2.0.a1 Prupe.3G260900_v2.0.a1 Prupe.3G261100_v2.0.a1 Prupe.3G261100_v2.0.a1 Prupe.3G261100_v2.0.a1
pyrus_communis pycom01g18590 pycom08g18530 pycom09g03820 pycom09g04260 pycom111g03590 pycom111g04940 pycom111g05070 pycom17g05450
rosa_chinensis RchiOBHm_Chr1g0315341 RchiOBHm_Chr2g0166831
rosa_laevigata RLG00000009567 RLG00000009568 RLG00000013046 RLG00000018053 RLG00000018054 RLG00000018055 RLG00000021644 RLG00000021647 RLG00000021648 RLG00000021651 RLG00000021653 RLG00000021655 RLG00000021656 RLG00000021661 RLG00000021668
rosa_multiflora Rmu_co8319251.1_g000001 Rmu_sc0000612.1_g000004 Rmu_sc0000612.1_g000013 Rmu_sc0000612.1_g000022 Rmu_sc0000690.1_g000022 Rmu_sc0000768.1_g000029 Rmu_sc0000820.1_g000021 Rmu_sc0000949.1_g000012 Rmu_sc0001432.1_g000005 Rmu_sc0002322.1_g000037 Rmu_sc0002539.1_g000106 Rmu_sc0002735.1_g000005 Rmu_sc0002966.1_g000004 Rmu_sc0002966.1_g000006 Rmu_sc0002966.1_g000007 Rmu_sc0005344.1_g000001 Rmu_sc0006325.1_g000034 Rmu_sc0006838.1_g000007 Rmu_sc0006838.1_g000019 Rmu_sc0008490.1_g000023 Rmu_sc0008530.1_g000001 Rmu_sc0012743.1_g000005 Rmu_sc0013600.1_g000004 Rmu_sc0016890.1_g000002
rosa_roxburghii Rroxscaffold_1G00012720 Rroxscaffold_1G00013420 Rroxscaffold_2G00083970 Rroxscaffold_2G00084070 Rroxscaffold_2G00084110 Rroxscaffold_2G00084170 Rroxscaffold_2G00084190 Rroxscaffold_2G00084200 Rroxscaffold_2G00084210 Rroxscaffold_2G00084530 Rroxscaffold_2G00084540 Rroxscaffold_2G00129390 Rroxscaffold_2G00129510 Rroxscaffold_4G00327620 Rroxscaffold_4G00328190 Rroxscaffold_5G00340960 Rroxscaffold_5G00340970 Rroxscaffold_7G00187900
rosa_rugosa Rorug02G0186300 Rorug02G0186300 Rorug02G0186400 Rorug02G0525600 Rorug02G0525700 Rorug05G0573300 Rorug06G0132500
rosa_samantha Rh1CG075200 Rh1DG027600 Rh2AG240700 Rh2AG240800 Rh2AG591700 Rh2AG591900 Rh2AG592200 Rh2AG592300 Rh2AG592400 Rh2AG592600 Rh2AG592700 Rh2AG641300 Rh2BG254400 Rh2BG254700 Rh2BG254800 Rh2BG603800 Rh2CG244800 Rh2CG244900 Rh2DG248700 Rh2DG248800 Rh2DG614700 Rh2DG614800 Rh2DG614900 Rh2DG615100 Rh2DG615400 Rh2DG615500 Rh2DG615700 Rh2DG668300 Rh5AG445300 Rh5DG375200 Rh5DG476800 Rh6BG082200 Rh6BG247400 Rh6CG249200
rosa_wichuraiana Rw2G049330 Rw4G005360 Rw5G032950 Rw5G041590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 518, 551
Acc36I ACCTGC 2 cut(s) 518, 551
AccB1I GGYRCC 1 cut(s) 647
AccB7I CCANNNNNTGG 1 cut(s) 407
AccI GTMKAC 1 cut(s) 77
AccII CGCG 1 cut(s) 275
AciI CCGC 2 cut(s) 275, 360
AcoI YGGCCR 1 cut(s) 131
AcsI RAATTY 2 cut(s) 58, 403
AfaI GTAC 1 cut(s) 119
AfiI CCNNNNNNNGG 2 cut(s) 184, 407
AjnI CCWGG 1 cut(s) 183
AleI CACNNNNGTG 1 cut(s) 101
AluBI AGCT 3 cut(s) 308, 497, 628
AluI AGCT 3 cut(s) 308, 497, 628
Alw26I GTCTC 1 cut(s) 219
AoxI GGCC 1 cut(s) 131
ApeKI GCWGC 4 cut(s) 90, 93, 494, 593
ApoI RAATTY 2 cut(s) 58, 403
AsuHPI GGTGA 2 cut(s) 199, 349
AsuNHI GCTAGC 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 650
BanI GGYRCC 1 cut(s) 647
BbvCI CCTCAGC 1 cut(s) 347
BbvI GCAGC 4 cut(s) 102, 105, 506, 605
BceAI ACGGC 2 cut(s) 146, 196
BciT130I CCWGG 1 cut(s) 185
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 1 cut(s) 219
BfaI CTAG 4 cut(s) 17, 99, 170, 485
BfuAI ACCTGC 2 cut(s) 518, 551
BisI GCNGC 4 cut(s) 91, 94, 495, 594
BlsI GCNGC 4 cut(s) 92, 95, 496, 595
Bme1390I CCNGG 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 649
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 2 cut(s) 375, 666
BmtI GCTAGC 1 cut(s) 20
BplI GAGNNNNNCTC 2 cut(s) 165, 197
BpmI CTGGAG 1 cut(s) 167
Bpu10I CCTNAGC 1 cut(s) 347
BsaBI GATNNNNATC 1 cut(s) 389
BsaWI WCCGGW 1 cut(s) 200
Bsc4I CCNNNNNNNGG 2 cut(s) 184, 407
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 1 cut(s) 185
BseGI GGATG 3 cut(s) 65, 390, 681
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 2 cut(s) 184, 407
BseMII CTCAG 1 cut(s) 361
BseRI GAGGAG 1 cut(s) 170
BseSI GKGCMC 1 cut(s) 650
BseXI GCAGC 4 cut(s) 102, 105, 506, 605
Bsh1236I CGCG 1 cut(s) 275
Bsh1285I CGRYCG 1 cut(s) 201
BshFI GGCC 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 647
BsiEI CGRYCG 1 cut(s) 201
BsiSI CCGG 1 cut(s) 201
BslI CCNNNNNNNGG 2 cut(s) 184, 407
BsmAI GTCTC 1 cut(s) 219
BsmBI CGTCTC 1 cut(s) 219
BsnI GGCC 1 cut(s) 133
Bsp1286I GDGCHC 1 cut(s) 650
Bsp143I GATC 1 cut(s) 633
BspACI CCGC 2 cut(s) 275, 360
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 1 cut(s) 360
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 649
BspMI ACCTGC 2 cut(s) 518, 551
BspOI GCTAGC 1 cut(s) 20
BspT107I GGYRCC 1 cut(s) 647
BssMI GATC 1 cut(s) 633
BssNAI GTATAC 1 cut(s) 78
Bst1107I GTATAC 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 1 cut(s) 292
BstC8I GCNNGC 3 cut(s) 18, 30, 195
BstDEI CTNAG 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 182
BstF5I GGATG 3 cut(s) 65, 390, 681
BstFNI CGCG 1 cut(s) 275
BstKTI GATC 1 cut(s) 636
BstMAI GTCTC 1 cut(s) 219
BstMBI GATC 1 cut(s) 633
BstMCI CGRYCG 1 cut(s) 201
BstMWI GCNNNNNNNGC 2 cut(s) 38, 357
BstNI CCWGG 1 cut(s) 185
BstNSI RCATGY 2 cut(s) 197, 429
BstSCI CCNGG 1 cut(s) 183
BstSLI GKGCMC 1 cut(s) 650
BstUI CGCG 1 cut(s) 275
BstV1I GCAGC 4 cut(s) 102, 105, 506, 605
BstZ17I GTATAC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 3 cut(s) 65, 390, 681
BveI ACCTGC 2 cut(s) 518, 551
Cac8I GCNNGC 3 cut(s) 18, 30, 195
Csp6I GTAC 1 cut(s) 118
CviAII CATG 4 cut(s) 166, 194, 426, 539
CviJI RGCY 6 cut(s) 16, 133, 308, 351, 497, 628
CviKI_1 RGCY 6 cut(s) 16, 133, 308, 351, 497, 628
CviQI GTAC 1 cut(s) 118
DdeI CTNAG 1 cut(s) 347
DpnI GATC 1 cut(s) 635
DpnII GATC 1 cut(s) 633
EaeI YGGCCR 1 cut(s) 131
EcoNI CCTNNNNNAGG 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 183
Esp3I CGTCTC 1 cut(s) 219
FaeI CATG 4 cut(s) 169, 197, 429, 542
FatI CATG 4 cut(s) 165, 193, 425, 538
FauI CCCGC 1 cut(s) 268
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 4 cut(s) 91, 94, 495, 594
FokI GGATG 2 cut(s) 52, 377
Fsp4HI GCNGC 4 cut(s) 91, 94, 495, 594
FspBI CTAG 4 cut(s) 17, 99, 170, 485
GluI GCNGC 4 cut(s) 91, 94, 495, 594
GsuI CTGGAG 1 cut(s) 167
HaeIII GGCC 1 cut(s) 133
HapII CCGG 1 cut(s) 201
Hin1II CATG 4 cut(s) 169, 197, 429, 542
HindIII AAGCTT 1 cut(s) 306
HinfI GANTC 2 cut(s) 174, 376
HpaII CCGG 1 cut(s) 201
HphI GGTGA 2 cut(s) 199, 349
Hpy166II GTNNAC 3 cut(s) 78, 340, 417
Hpy8I GTNNAC 3 cut(s) 78, 340, 417
HpyAV CCTTC 4 cut(s) 517, 550, 583, 616
HpyCH4III ACNGT 1 cut(s) 292
HpyCH4V TGCA 2 cut(s) 371, 494
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 357
HpyF3I CTNAG 1 cut(s) 347
Hsp92II CATG 4 cut(s) 169, 197, 429, 542
Ksp22I TGATCA 1 cut(s) 633
Kzo9I GATC 1 cut(s) 633
LmnI GCTCC 1 cut(s) 625
LpnPI CCDG 7 cut(s) 147, 170, 197, 214, 513, 546, 680
Lsp1109I GCAGC 4 cut(s) 102, 105, 506, 605
LweI GCATC 2 cut(s) 375, 666
MaeI CTAG 4 cut(s) 17, 99, 170, 485
MaeIII GTNAC 5 cut(s) 223, 454, 545, 578, 611
MalI GATC 1 cut(s) 635
MboI GATC 1 cut(s) 633
MboII GAAGA 1 cut(s) 665
MhlI GDGCHC 1 cut(s) 650
MluCI AATT 5 cut(s) 58, 121, 403, 450, 597
MlyI GAGTC 1 cut(s) 183
MmeI TCCRAC 1 cut(s) 336
MnlI CCTC 7 cut(s) 59, 100, 188, 191, 356, 362, 404
MslI CAYNNNNRTG 1 cut(s) 101
MspA1I CMGCKG 1 cut(s) 497
MspI CCGG 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 38, 357
NdeII GATC 1 cut(s) 633
NheI GCTAGC 1 cut(s) 16
NlaIII CATG 4 cut(s) 169, 197, 429, 542
NlaIV GGNNCC 1 cut(s) 649
NmuCI GTSAC 3 cut(s) 454, 578, 611
NspI RCATGY 2 cut(s) 197, 429
OliI CACNNNNGTG 1 cut(s) 101
PaeI GCATGC 1 cut(s) 197
PaqCI CACCTGC 2 cut(s) 518, 551
PcsI WCGNNNNNNNCGW 2 cut(s) 508, 607
PfeI GAWTC 1 cut(s) 376
PflFI GACNNNGTC 1 cut(s) 460
PflMI CCANNNNNTGG 1 cut(s) 407
PkrI GCNGC 4 cut(s) 92, 95, 496, 595
PleI GAGTC 1 cut(s) 182
PpsI GAGTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 649
PsyI GACNNNGTC 1 cut(s) 460
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 1 cut(s) 119
RsaNI GTAC 1 cut(s) 118
RseI CAYNNNNRTG 1 cut(s) 101
SatI GCNGC 4 cut(s) 91, 94, 495, 594
Sau3AI GATC 1 cut(s) 633
SchI GAGTC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 650
SetI ASST 8 cut(s) 189, 310, 348, 490, 499, 532, 565, 630
SfaNI GCATC 2 cut(s) 375, 666
SmiMI CAYNNNNRTG 1 cut(s) 101
SphI GCATGC 1 cut(s) 197
Sse9I AATT 5 cut(s) 58, 121, 403, 450, 597
SsiI CCGC 2 cut(s) 275, 360
SspMI CTAG 4 cut(s) 17, 99, 170, 485
StyD4I CCNGG 1 cut(s) 183
TaaI ACNGT 1 cut(s) 292
TasI AATT 5 cut(s) 58, 121, 403, 450, 597
TatI WGTACW 1 cut(s) 117
TfiI GAWTC 1 cut(s) 376
TseFI GTSAC 3 cut(s) 454, 578, 611
TseI GCWGC 4 cut(s) 90, 93, 494, 593
Tsp45I GTSAC 3 cut(s) 454, 578, 611
TspDTI ATGAA 4 cut(s) 536, 569, 602, 635
TspGWI ACGGA 1 cut(s) 599
Tth111I GACNNNGTC 1 cut(s) 460
Van91I CCANNNNNTGG 1 cut(s) 407
XagI CCTNNNNNAGG 1 cut(s) 182
XapI RAATTY 2 cut(s) 58, 403
XceI RCATGY 2 cut(s) 197, 429
XcmI CCANNNNNNNNNTGG 1 cut(s) 408
XmiI GTMKAC 1 cut(s) 77
XspI CTAG 4 cut(s) 17, 99, 170, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.