MD01G1031100.v1.1

Calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0001949
Physical Location & Seq
Forward (+)
10912760 .. 10939123
26364 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1031100.v1.1.491

Sequence Viewer

Length: 3324 bp
ATGGCGGAGATCAAGCGCTACGGTCTTGGTAATCAATTGGATATTGCGCAGATACTCTTAGAAGCTAAACATCGCTGGCTACGCCCGGCTGAAATATGTGAAATTCTTCGAAATTTTCAAAAATTTGACATTTCTTCAGAGCCTGCAAATATGCCACCCGGTGGATCACTTTTTCTATTTGATCGGAAGGTGCTTAGATATTTCAGGAAAGATGGCCATAACTGGAGGAAGAAAAAAGATGGAAAGACAGTGAGGGAAGCTCATGAGAGACTTAAGGCTGGAAGTGTTGATGTCTTGCACTGCTATTATGCACATGGAGAAGAAAATGAAAATTTCCAAAGACGCAGTTATTGGATGCTTGAGGAGGATCTGCAACACATAGTTCTTGTCCACTACCGGGAAGTTAAGGGAAATAGGACAAACTATAACCATTTTAAAGGCACTGAAGAAGCTGTTCCTTATTCCCATGAAACTAAAGAAATTGCAATCAATTCTGAGATGGACAATTCTGTTTCTTCTAGTTTCAATCCAAATAATTTCCAAATGCGTTCCCAAGCTGCTGGTACAACAAGCTTGAGTAGCGCTCAAGCATCAGAGTTCGAAGATGCTGAGTCAGCATATAATCACCAAGCAAGTTCCCAGTTACAACCTTTTCTCGAATTACTACAGCCTAAGGCTGAGAAGATCAATGCTGGATTCTCTAATGCATTCTATCCGATGTCATTTTCAAAAGAATATCAAGAAAAATTGTCAGCCGTTCCTGGGGTGAATTTTAGCTCACGTACTCAAGCATATAGGAAGGAAGATGGTAATGATGCTGGCGTTACCTATGATCCCAGGAAAAATCCTAACTCCACAGTATGGGATGGTGCCTTGGGGAATATTACTACTGGCCTTCAATCTTTGCCTTTCCAGCCATCAATTTCAGCAACACATTCTGATTCAATGGGAATTATCTCTCAGCAAGAGAATGAGACGCTTGGGCACCTTTTCACAGACAACTTTGGTAAAAAGCAGATGTACGAGGATAAACCAAGGGTCCAACAAAGCTGGCAGACTTTGGAAGCCAACTCTTCTGGTTCATCCAGCTGGCCTGTGGATCAGAACTTGCATTCAGACACAGCATATGATGTCACCACAAGGTTCCGTGGAGGAGTCGATTCTAATTTACTCAATTCTCCGGTGTGCAGTGTGGATTCTGAGAAGACAAATAATTTTTCTATGCCAAATGACCTTCAAATACAGCCTTCAAACACTGAGCAAGAATATCATCTGAAGTCTATTTCAAAGAGAAATGAAACCATAGAGGGAAAATACAATCACACTTTTGCTACAAAGCCTTTGTTAGATGAAGGCTTGAAGAAGCTTGACAGTTTCAACCGATGGATGAGCAAAGAACTTGGAGACGTGGACGAGACACACACACAGTCCAATTCAGAGACGTACTGGGATACTGTTGAAAGTGACAATGGGGTCGATGAGTCCAGTATTCCTCTTCAAGTACGCTTGGATAGCTATATGCTGGGGCCTTCTCTTTCCCAGGACCAGCTCTTTAGCATTATTGATTTCTCACCAAACTGGGCATACGAAAACTCTGTAATCAAGGTTCTGATCACAGGAAGATTTTTGAAGAGTCAACAAGCAGAAAGTTGTAAGTGGTCATGCATGTTTGGCGAAGTTGAAGTTCCTGCTGAGGTTATAGCTGATGGTGTTCTTCGTTGTTATACTCCCATCCATAAGGCTGGGAGAATTCCTTTCTACGTGACATGTTCCAATAGGCTGGCTTGTAGTGAAATCAGGGAATTTGAATACCGAGTCGGGCAAATACCAGATTATGATGTTAAAGATGATTACAGTGGCTGCACAAATGAAATATTAAATATGCGCTTTGGAAAATTACTGTCTTTAAGCTCTAGCTCTCCAACTTTTGATCCCACCAGCGTAGCAGAGAATTCTGAGATAATCAGTAAAATTGATTTGTTGCTGAAAAATGACAATGGTGAGTGGGACAAGATGCTACAACTTACGTCAGATGAGGACTTTTCCTTGGAGAGAGTAGAGGACCAGTTGCTTCAACAGCTACTTAAAGAGAAGTTGCTTGCATGGCTCTTGCAAAAGCTGGCTGCTGGTGGAAAAGGCCCTAGTGTACTAGACGAGGGTGGCCAAGGTGTATTACATTTTGGAGCTGCTCTTGGCTATGATTGGGTCTTACTGCCCACAATAACTGCCGGAGTCAGTGTCAATTTTCGTGACATTAATGGATGGACAGCTCTTCACTGGGCAGCTTTTTGTGGCAGAGAGCGCACAGTTGCTTCCCTCATCTCTCTTGGTGCAGCTCCCGGACTATTAACAGATCCAAAAACCAAATATCCTAATGGAAGAACACCTGCAGACCTAGCTTCTGCACAGGGCCACAAGGGAATTGCTGGTTATCTTGCAGAATCTGCTTTGAGTGACCAACTTTCATTTCTTAATTTGGATATCAAGGAAGACAATAATGCTGATATTTCAGGGGCCAATGCAGTACGAACAGTTTCAGAACAAATTGCAACTCCTATCGGGAATGGGGATTTAACGGATGGGCTTTCCCTGAGGGATTCTTTAACTGCTGTCTGTAATGCTACCCAAGCTGCTGCTCGTATTCATCAAGTGTTCAGGGTAAAATCTTTCCAGCTGAAGCAGTTGAAAGAATATGGTAGTGATAACTTTGGAATTTCAGATGAAGATGCTCTATCAATGATTGCAGTCAAGTCACATAAGCCAGGGAAACGTGATGAGCATGTGGATGCTGCTGCAATTCGAATACAAAATAAGTTCCGTAGTTGGAAGGGTAGAAAAGATTATTTGATAATCCGACAACGTATTGTCAAAATTCAGGCTCATGTAAGAGGCCACCAGGTGAGGAAAAACTACAGAAAGATAGTCTGGTCCGTAGGAATTGTAGAGAAGATAATTTTGCGTTGGAGAAGAAAAGGAAGTGGTTTGCGTGGGTTCAAGCCAGAACCACTTACTGAAGCCCCCAGCATGCAAGCTTCATCATCGAAGGATGATGATTATGATGTACTGAAAGAAGGAAGGAAGCAAACAGAGCAAAGGTTGCAAAAGGCGCTTGCCAGGGTGAAATCCATGGTTCAATATCCTGAAGCAAGAGATCAATACTGTCGGCTGCTGAATGTTGTCACTGAGATACAGGAAACCAAGGTGGTATATGATAGTGGTACGACTAGTTCAGAAGGGAGAGTCGATATGGATCACAACCTCATAGATATTGCAGCATTGTTGGACGAAGAAGACATTTACATGCCTACAGAAGCTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 CG-1 IQ TIG

Protein Analysis

1108

Amino Acids

124.44

Weight (kDa)

5.73

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 21 - 134 2.9e-50 CG-1 domain
Ank_2 PF12796 727 - 815 7.5e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2395
AasI GACNNNNNNGTC 1 cut(s) 1472
Acc16I TGCGCA 1 cut(s) 48
Acc36I ACCTGC 1 cut(s) 2395
AccB1I GGYRCC 2 cut(s) 869, 984
AccB7I CCANNNNNTGG 2 cut(s) 161, 861
AciI CCGC 1 cut(s) 5
AclWI GGATC 7 cut(s) 172, 375, 827, 1107, 1925, 2348, 3258
AcoI YGGCCR 2 cut(s) 214, 2161
AcuI CTGAAG 6 cut(s) 120, 465, 1295, 2696, 3033, 3162
AdeI CACNNNGTG 2 cut(s) 161, 2899
AfaI GTAC 9 cut(s) 565, 784, 1022, 1445, 1503, 2148, 2526, 3063, 3220
AfeI AGCGCT 2 cut(s) 17, 583
AfiI CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
AflII CTTAAG 1 cut(s) 272
AflIII ACRYGT 1 cut(s) 1766
AhlI ACTAGT 1 cut(s) 3224
AjiI CACGTC 1 cut(s) 1408
AjnI CCWGG 6 cut(s) 760, 836, 1539, 2761, 2895, 3113
AjuI GAANNNNNNNTTGG 2 cut(s) 2451, 2483
Alw26I GTCTC 5 cut(s) 262, 968, 1398, 1409, 1433
AlwI GGATC 7 cut(s) 172, 375, 827, 1107, 1925, 2348, 3258
AlwNI CAGNNNCTG 3 cut(s) 143, 1860, 2444
Aor51HI AGCGCT 2 cut(s) 17, 583
AoxI GGCC 9 cut(s) 214, 892, 1091, 1526, 2137, 2161, 2410, 2514, 2890
AseI ATTAAT 1 cut(s) 2256
Asp700I GAANNNNTTC 3 cut(s) 105, 453, 2533
AspLEI GCGC 6 cut(s) 18, 49, 584, 1887, 2304, 3109
AspS9I GGNCC 8 cut(s) 1039, 1526, 1543, 2062, 2138, 2410, 2514, 2928
AsuC2I CCSGG 4 cut(s) 86, 159, 398, 2340
AsuHPI GGTGA 7 cut(s) 617, 778, 1126, 1563, 2012, 2911, 3130
AsuII TTCGAA 3 cut(s) 109, 600, 2800
AvaII GGWCC 4 cut(s) 1039, 1543, 2062, 2928
AxyI CCTNAGG 2 cut(s) 672, 2591
BaeGI GKGCMC 1 cut(s) 987
BalI TGGCCA 2 cut(s) 216, 2163
BanI GGYRCC 2 cut(s) 869, 984
BbsI GAAGAC 3 cut(s) 1211, 2496, 3297
BbvCI CCTCAGC 1 cut(s) 1692
BceAI ACGGC 1 cut(s) 740
BciT130I CCWGG 6 cut(s) 762, 838, 1541, 2763, 2897, 3115
BciVI GTATCC 1 cut(s) 1444
BclI TGATCA 1 cut(s) 1611
BcnI CCSGG 4 cut(s) 86, 159, 398, 2340
BcoDI GTCTC 5 cut(s) 262, 968, 1398, 1409, 1433
BcuI ACTAGT 1 cut(s) 3224
BfaI CTAG 6 cut(s) 519, 1914, 2142, 2150, 2396, 3225
BfmI CTRYAG 4 cut(s) 665, 2388, 2911, 3306
BfoI RGCGCY 3 cut(s) 19, 585, 3110
BfrI CTTAAG 1 cut(s) 272
BfuAI ACCTGC 1 cut(s) 2395
BfuI GTATCC 1 cut(s) 1444
Bme18I GGWCC 4 cut(s) 1039, 1543, 2062, 2928
BmgBI CACGTC 1 cut(s) 1408
BmgT120I GGNCC 8 cut(s) 1039, 1526, 1543, 2062, 2138, 2410, 2514, 2928
BmiI GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1527, 2515
BmrI ACTGGG 4 cut(s) 634, 1456, 1588, 2287
BmsI GCATC 7 cut(s) 345, 595, 599, 805, 2004, 2716, 2776
BmuI ACTGGG 4 cut(s) 634, 1456, 1588, 2287
BpiI GAAGAC 3 cut(s) 1211, 2496, 3297
BplI GAGNNNNNCTC 4 cut(s) 244, 276, 568, 600
BpmI CTGGAG 1 cut(s) 244
Bpu10I CCTNAGC 1 cut(s) 1692
Bpu14I TTCGAA 3 cut(s) 109, 600, 2800
BpuEI CTTGAG 4 cut(s) 380, 570, 595, 771
BpuMI CCSGG 4 cut(s) 86, 159, 398, 2340
BsaAI YACGTR 2 cut(s) 782, 1762
BsaBI GATNNNNATC 1 cut(s) 3249
BsaWI WCCGGW 1 cut(s) 1180
BsaXI ACNNNNNCTCC 3 cut(s) 2223, 2253, 3298
Bsc4I CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
Bse1I ACTGG 8 cut(s) 227, 640, 895, 1451, 1485, 1583, 2065, 2282
Bse21I CCTNAGG 2 cut(s) 672, 2591
Bse8I GATNNNNATC 1 cut(s) 3249
BseBI CCWGG 6 cut(s) 762, 838, 1541, 2763, 2897, 3115
BseGI GGATG 9 cut(s) 360, 871, 1082, 1392, 1731, 2267, 2584, 2791, 3052
BseJI GATNNNNATC 1 cut(s) 3249
BseLI CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
BseNI ACTGG 8 cut(s) 227, 640, 895, 1451, 1485, 1583, 2065, 2282
BseRI GAGGAG 2 cut(s) 377, 1167
BseSI GKGCMC 1 cut(s) 987
BseYI CCCAGC 3 cut(s) 1522, 1742, 3020
BsgI GTGCAG 4 cut(s) 1207, 1846, 2352, 2388
BshFI GGCC 9 cut(s) 216, 894, 1093, 1528, 2139, 2163, 2412, 2516, 2892
BshNI GGYRCC 2 cut(s) 869, 984
BsiSI CCGG 6 cut(s) 86, 159, 397, 1181, 2229, 2340
BslFI GGGAC 1 cut(s) 2021
BslI CCNNNNNNNGG 4 cut(s) 161, 397, 762, 861
BsmAI GTCTC 5 cut(s) 262, 968, 1398, 1409, 1433
BsmBI CGTCTC 3 cut(s) 968, 1398, 1433
BsmFI GGGAC 1 cut(s) 2021
BsmI GAATGC 2 cut(s) 707, 1111
BsnI GGCC 9 cut(s) 216, 894, 1093, 1528, 2139, 2163, 2412, 2516, 2892
Bsp119I TTCGAA 3 cut(s) 109, 600, 2800
Bsp1286I GDGCHC 1 cut(s) 987
Bsp19I CCATGG 1 cut(s) 3126
BspACI CCGC 1 cut(s) 5
BspANI GGCC 9 cut(s) 216, 894, 1093, 1528, 2139, 2163, 2412, 2516, 2892
BspHI TCATGA 1 cut(s) 262
BspLI GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1527, 2515
BspMAI CTGCAG 1 cut(s) 2392
BspMI ACCTGC 1 cut(s) 2395
BspPI GGATC 7 cut(s) 172, 375, 827, 1107, 1925, 2348, 3258
BspQI GCTCTTC 1 cut(s) 2277
BspT104I TTCGAA 3 cut(s) 109, 600, 2800
BspT107I GGYRCC 2 cut(s) 869, 984
BspTI CTTAAG 1 cut(s) 272
BsrI ACTGG 8 cut(s) 227, 640, 895, 1451, 1485, 1583, 2065, 2282
BssT1I CCWWGG 6 cut(s) 873, 1034, 2046, 2164, 3126, 3198
Bst2UI CCWGG 6 cut(s) 762, 838, 1541, 2763, 2897, 3115
Bst6I CTCTTC 4 cut(s) 1078, 1500, 1625, 2277
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 2 cut(s) 2444, 3097
BstBAI YACGTR 2 cut(s) 782, 1762
BstBI TTCGAA 3 cut(s) 109, 600, 2800
BstDSI CCRYGG 2 cut(s) 1147, 3126
BstF5I GGATG 9 cut(s) 360, 871, 1082, 1392, 1731, 2267, 2584, 2791, 3052
BstH2I RGCGCY 3 cut(s) 19, 585, 3110
BstHHI GCGC 6 cut(s) 18, 49, 584, 1887, 2304, 3109
BstMAI GTCTC 5 cut(s) 262, 968, 1398, 1409, 1433
BstNI CCWGG 6 cut(s) 762, 838, 1541, 2763, 2897, 3115
BstNSI RCATGY 5 cut(s) 1669, 1770, 2783, 3028, 3304
BstSFI CTRYAG 4 cut(s) 665, 2388, 2911, 3306
BstSLI GKGCMC 1 cut(s) 987
BstV2I GAAGAC 3 cut(s) 1211, 2496, 3297
BstX2I RGATCY 2 cut(s) 367, 2353
BstXI CCANNNNNNTGG 3 cut(s) 560, 1742, 1780
BstYI RGATCY 2 cut(s) 367, 2353
Bsu36I CCTNAGG 2 cut(s) 672, 2591
BsuI GTATCC 1 cut(s) 1444
BsuRI GGCC 9 cut(s) 216, 894, 1093, 1528, 2139, 2163, 2412, 2516, 2892
BtgI CCRYGG 2 cut(s) 1147, 3126
BtgZI GCGATG 1 cut(s) 56
BtrI CACGTC 1 cut(s) 1408
BtsCI GGATG 9 cut(s) 360, 871, 1082, 1392, 1731, 2267, 2584, 2791, 3052
BtsI GCAGTG 2 cut(s) 298, 1195
BtsIMutI CAGTG 9 cut(s) 255, 298, 441, 1195, 1254, 1861, 2242, 2275, 3180
BveI ACCTGC 1 cut(s) 2395
CaiI CAGNNNCTG 3 cut(s) 143, 1860, 2444
CciI TCATGA 1 cut(s) 262
CfoI GCGC 6 cut(s) 18, 49, 584, 1887, 2304, 3109
Cfr13I GGNCC 8 cut(s) 1039, 1526, 1543, 2062, 2138, 2410, 2514, 2928
CseI GACGC 2 cut(s) 351, 985
CsiI ACCWGGT 1 cut(s) 2895
Csp6I GTAC 9 cut(s) 564, 783, 1021, 1444, 1502, 2147, 2525, 3062, 3219
CviQI GTAC 9 cut(s) 564, 783, 1021, 1444, 1502, 2147, 2525, 3062, 3219
DraI TTTAAA 1 cut(s) 436
DraIII CACNNNGTG 2 cut(s) 161, 2899
DrdI GACNNNNNNGTC 1 cut(s) 1472
DseDI GACNNNNNNGTC 1 cut(s) 1472
EaeI YGGCCR 2 cut(s) 214, 2161
Eam1104I CTCTTC 4 cut(s) 1078, 1500, 1625, 2277
EarI CTCTTC 4 cut(s) 1078, 1500, 1625, 2277
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 6 cut(s) 873, 1034, 2046, 2164, 3126, 3198
Eco32I GATATC 1 cut(s) 2482
Eco47I GGWCC 4 cut(s) 1039, 1543, 2062, 2928
Eco47III AGCGCT 2 cut(s) 17, 583
Eco57I CTGAAG 6 cut(s) 120, 465, 1295, 2696, 3033, 3162
Eco81I CCTNAGG 2 cut(s) 672, 2591
EcoO109I RGGNCCY 2 cut(s) 1526, 2138
EcoRI GAATTC 2 cut(s) 1749, 1951
EcoRII CCWGG 6 cut(s) 760, 836, 1539, 2761, 2895, 3113
EcoRV GATATC 1 cut(s) 2482
EcoT14I CCWWGG 6 cut(s) 873, 1034, 2046, 2164, 3126, 3198
EcoT22I ATGCAT 2 cut(s) 709, 1667
ErhI CCWWGG 6 cut(s) 873, 1034, 2046, 2164, 3126, 3198
Esp3I CGTCTC 3 cut(s) 968, 1398, 1433
FaqI GGGAC 1 cut(s) 2021
FauNDI CATATG 1 cut(s) 1126
FbaI TGATCA 1 cut(s) 1611
FokI GGATG 9 cut(s) 367, 878, 1069, 1399, 1718, 2274, 2591, 2798, 3059
FspBI CTAG 6 cut(s) 519, 1914, 2142, 2150, 2396, 3225
FspI TGCGCA 1 cut(s) 48
GlaI GCGC 6 cut(s) 17, 48, 583, 1886, 2303, 3108
GsaI CCCAGC 3 cut(s) 1526, 1746, 3024
GsuI CTGGAG 1 cut(s) 244
HaeII RGCGCY 3 cut(s) 19, 585, 3110
HaeIII GGCC 9 cut(s) 216, 894, 1093, 1528, 2139, 2163, 2412, 2516, 2892
HapII CCGG 6 cut(s) 86, 159, 397, 1181, 2229, 2340
HgaI GACGC 2 cut(s) 351, 985
HhaI GCGC 6 cut(s) 18, 49, 584, 1887, 2304, 3109
Hin6I GCGC 6 cut(s) 16, 47, 582, 1885, 2302, 3107
HinP1I GCGC 6 cut(s) 16, 47, 582, 1885, 2302, 3107
HincII GTYRAC 1 cut(s) 1637
HindII GTYRAC 1 cut(s) 1637
HindIII AAGCTT 3 cut(s) 571, 1364, 3030
HpaII CCGG 6 cut(s) 86, 159, 397, 1181, 2229, 2340
HphI GGTGA 7 cut(s) 617, 778, 1126, 1563, 2012, 2911, 3130
Hpy166II GTNNAC 4 cut(s) 391, 1411, 1637, 2147
Hpy188III TCNNGA 7 cut(s) 205, 263, 656, 740, 2249, 2560, 3140
Hpy8I GTNNAC 4 cut(s) 391, 1411, 1637, 2147
HpyCH4IV ACGT 7 cut(s) 781, 1407, 1442, 1761, 2027, 2770, 2860
HpySE526I ACGT 7 cut(s) 781, 1407, 1442, 1761, 2027, 2770, 2860
HspAI GCGC 6 cut(s) 16, 47, 582, 1885, 2302, 3107
Ksp22I TGATCA 1 cut(s) 1611
LguI GCTCTTC 1 cut(s) 2277
LmnI GCTCC 3 cut(s) 2183, 2341, 3319
LweI GCATC 7 cut(s) 345, 595, 599, 805, 2004, 2716, 2776
MabI ACCWGGT 1 cut(s) 2895
MaeI CTAG 6 cut(s) 519, 1914, 2142, 2150, 2396, 3225
MaeII ACGT 7 cut(s) 781, 1407, 1442, 1761, 2027, 2770, 2860
MaeIII GTNAC 9 cut(s) 642, 823, 1132, 1463, 1762, 2249, 2453, 2751, 3178
MfeI CAATTG 1 cut(s) 35
MflI RGATCY 2 cut(s) 367, 2353
MhlI GDGCHC 1 cut(s) 987
MlsI TGGCCA 2 cut(s) 216, 2163
MluNI TGGCCA 2 cut(s) 216, 2163
MlyI GAGTC 7 cut(s) 620, 1164, 1490, 1642, 1824, 2241, 3249
MmeI TCCRAC 6 cut(s) 1066, 1946, 2804, 2878, 2942, 3261
Mox20I TGGCCA 2 cut(s) 216, 2163
Mph1103I ATGCAT 2 cut(s) 709, 1667
MroXI GAANNNNTTC 3 cut(s) 105, 453, 2533
MscI TGGCCA 2 cut(s) 216, 2163
MslI CAYNNNNRTG 2 cut(s) 2784, 3299
Msp20I TGGCCA 2 cut(s) 216, 2163
MspA1I CMGCKG 2 cut(s) 1089, 2674
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 6 cut(s) 86, 159, 397, 1181, 2229, 2340
MunI CAATTG 1 cut(s) 35
Mva1269I GAATGC 2 cut(s) 707, 1111
MvaI CCWGG 6 cut(s) 762, 838, 1541, 2763, 2897, 3115
NciI CCSGG 4 cut(s) 86, 159, 398, 2340
NcoI CCATGG 1 cut(s) 3126
NdeI CATATG 1 cut(s) 1126
NlaIV GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1527, 2515
NmuCI GTSAC 7 cut(s) 1132, 1463, 1762, 2249, 2453, 2751, 3178
NsbI TGCGCA 1 cut(s) 48
NsiI ATGCAT 2 cut(s) 709, 1667
NspI RCATGY 5 cut(s) 1669, 1770, 2783, 3028, 3304
NspV TTCGAA 3 cut(s) 109, 600, 2800
PaeI GCATGC 1 cut(s) 3028
PagI TCATGA 1 cut(s) 262
PaqCI CACCTGC 1 cut(s) 2395
PciI ACATGT 1 cut(s) 1766
PciSI GCTCTTC 1 cut(s) 2277
PctI GAATGC 2 cut(s) 707, 1111
PdmI GAANNNNTTC 3 cut(s) 105, 453, 2533
PfeI GAWTC 6 cut(s) 696, 941, 1160, 1196, 2441, 2597
PflMI CCANNNNNTGG 2 cut(s) 161, 861
PfoI TCCNGGA 1 cut(s) 2338
PleI GAGTC 7 cut(s) 619, 1163, 1489, 1641, 1823, 2240, 3248
PpsI GAGTC 7 cut(s) 619, 1163, 1489, 1641, 1823, 2240, 3248
Ppu21I YACGTR 2 cut(s) 782, 1762
PscI ACATGT 1 cut(s) 1766
PshBI ATTAAT 1 cut(s) 2256
Psp6I CCWGG 6 cut(s) 760, 836, 1539, 2761, 2895, 3113
PspFI CCCAGC 3 cut(s) 1522, 1742, 3020
PspGI CCWGG 6 cut(s) 760, 836, 1539, 2761, 2895, 3113
PspN4I GGNNCC 6 cut(s) 871, 986, 1040, 1145, 1527, 2515
PspPI GGNCC 8 cut(s) 1039, 1526, 1543, 2062, 2138, 2410, 2514, 2928
PsrI GAACNNNNNNTAC 4 cut(s) 1590, 1622, 3211, 3243
PstI CTGCAG 1 cut(s) 2392
PstNI CAGNNNCTG 3 cut(s) 143, 1860, 2444
PsuI RGATCY 2 cut(s) 367, 2353
PvuII CAGCTG 2 cut(s) 1089, 2674
RsaI GTAC 9 cut(s) 565, 784, 1022, 1445, 1503, 2148, 2526, 3063, 3220
RsaNI GTAC 9 cut(s) 564, 783, 1021, 1444, 1502, 2147, 2525, 3062, 3219
RseI CAYNNNNRTG 2 cut(s) 2784, 3299
SapI GCTCTTC 1 cut(s) 2277
Sau96I GGNCC 8 cut(s) 1039, 1526, 1543, 2062, 2138, 2410, 2514, 2928
SchI GAGTC 7 cut(s) 620, 1164, 1490, 1642, 1824, 2241, 3249
SduI GDGCHC 1 cut(s) 987
SexAI ACCWGGT 1 cut(s) 2895
SfaNI GCATC 7 cut(s) 345, 595, 599, 805, 2004, 2716, 2776
SfcI CTRYAG 4 cut(s) 665, 2388, 2911, 3306
SfuI TTCGAA 3 cut(s) 109, 600, 2800
SinI GGWCC 4 cut(s) 1039, 1543, 2062, 2928
SmiMI CAYNNNNRTG 2 cut(s) 2784, 3299
SmlI CTYRAG 5 cut(s) 272, 359, 574, 585, 786
SmoI CTYRAG 5 cut(s) 272, 359, 574, 585, 786
SpeI ACTAGT 1 cut(s) 3224
SphI GCATGC 1 cut(s) 3028
SsiI CCGC 1 cut(s) 5
SspI AATATT 2 cut(s) 883, 1875
SspMI CTAG 6 cut(s) 519, 1914, 2142, 2150, 2396, 3225
StyI CCWWGG 6 cut(s) 873, 1034, 2046, 2164, 3126, 3198
TaiI ACGT 7 cut(s) 784, 1410, 1445, 1764, 2030, 2773, 2863
TaqI TCGA 8 cut(s) 109, 600, 657, 1158, 1476, 2800, 3041, 3243
TatI WGTACW 2 cut(s) 2146, 3061
TfiI GAWTC 6 cut(s) 696, 941, 1160, 1196, 2441, 2597
TscAI CASTG 9 cut(s) 255, 305, 448, 1195, 1261, 1861, 2242, 2282, 3187
TseFI GTSAC 7 cut(s) 1132, 1463, 1762, 2249, 2453, 2751, 3178
Tsp45I GTSAC 7 cut(s) 1132, 1463, 1762, 2249, 2453, 2751, 3178
TspGWI ACGGA 4 cut(s) 1136, 2591, 2807, 2920
TspRI CASTG 9 cut(s) 255, 305, 448, 1195, 1261, 1861, 2242, 2282, 3187
Van91I CCANNNNNTGG 2 cut(s) 161, 861
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 4 cut(s) 1039, 1543, 2062, 2928
VspI ATTAAT 1 cut(s) 2256
XceI RCATGY 5 cut(s) 1669, 1770, 2783, 3028, 3304
XcmI CCANNNNNNNNNTGG 1 cut(s) 1093
XmnI GAANNNNTTC 3 cut(s) 105, 453, 2533
XspI CTAG 6 cut(s) 519, 1914, 2142, 2150, 2396, 3225
Zsp2I ATGCAT 2 cut(s) 709, 1667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.