AT2G46420

Plant protein 1589 of unknown function (A_thal_3526)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001091
Physical Location & Seq
Forward (+)
19053470 .. 19057162
3693 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G46420.2

Sequence Viewer

Length: 1092 bp
ATGAAGAACGGACAGGAGTTGCAGTCTTCTACCCAAGTTTCACATGAGTCCCAAGGTGAACAGAAAGTTAATTTATCAGTAGAAGCTCCAATACAAGATTCTGGTTCTGTATCTGCCTCAAGTAATGACGGTAGGAAAGTGTCAAGACAAGATATTGAACTTGTCCAAAATTTGATAGAAAGATGTCTCCAGTTGTACATGAGCAGAGATGAGGTCGTAAAGACACTCTTGACCCGTGCAAGGATTGACCCTGGTTTTACAACATTAGTATGGCAAAAACTGGAAGAAGAAAACGCAGAGTTTTTCAGGGCATATTACATCAGACTGAAACTGAAGAAGCAAATAGTTGTATTCAATCATTTGCTTGAGCATCAGTATCACCTCACGAAGTATAATGTACATTCAAAAGTTCCTCTGGTCCCAATGCAGAACGGTATTCATCCTATGGCATCTGTGAACAACATGCCCATGGGATACCCAGTACTACAACATCCTCAGATGCATGCTCAAGGTCATCCCCATCTTGATCCTATGAGCTGTGGAATGTCAAGCTGCCATGTGGTTAATGGAGTCCCTGCACCTGCTAATTTCCAACCAATGAGGATCAACTCAGGGAATGATATGGTAATAGATACAACCATGGCTGAACCAACTCCAATGATTCCACCAAATAGTGGTATGTCGGATATGCCAGTAAGTCCAGCTTCTGTGGCGTCAAGCGGACACTTTCCATTTGCTGCTTCAGACATGTCAGGGATGGGAATGGACACGTCAGCTCTTGACTCTGCTTTCACATCTGATGTCGGAACTTCAGTAGGGTTACAACTTGGATCAGATGGAGGAGCTGGAAATTCTAGAGACCCTCTCAGGCCATTTGATCAGATACCTTGGAACTTTAGTCTCTCTGACCTCACTGCAGATTTGTCAAATCTTGGAGATTTAGGGGCTTTAGGAAACTATCCCGGTTCTCCTTTTCTGCCATCTGATTCAGAGATTTTACTTGACTCTCCCGAACAAGAGGACATAGATGAATTCTTTGTGGATTCGATTCCAGGACCTCCATGTTCTCAGTCAGAAGAAGACAAGTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
A_thal_3526

Protein Analysis

363

Amino Acids

39.56

Weight (kDa)

4.74

Isoelectric Point (pI)

58.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
A_thal_3526 PF09713 55 - 106 1.7e-25 Plant protein 1589 of unknown function (A_thal_3526)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 589
Acc36I ACCTGC 1 cut(s) 589
AccB7I CCANNNNNTGG 1 cut(s) 674
AciI CCGC 1 cut(s) 720
AclWI GGATC 3 cut(s) 521, 611, 838
AcsI RAATTY 3 cut(s) 169, 850, 1031
AcuI CTGAAG 3 cut(s) 353, 726, 795
AcyI GRCGYC 1 cut(s) 713
AfaI GTAC 3 cut(s) 197, 399, 483
AfiI CCNNNNNNNGG 2 cut(s) 240, 674
AflIII ACRYGT 2 cut(s) 747, 768
AgsI TTSAA 3 cut(s) 158, 355, 405
AjiI CACGTC 1 cut(s) 771
AjnI CCWGG 2 cut(s) 250, 1051
AjuI GAANNNNNNNTTGG 2 cut(s) 82, 114
AluBI AGCT 6 cut(s) 86, 537, 552, 704, 776, 845
AluI AGCT 6 cut(s) 86, 537, 552, 704, 776, 845
Alw26I GTCTC 3 cut(s) 191, 852, 905
AlwI GGATC 3 cut(s) 521, 611, 838
AlwNI CAGNNNCTG 1 cut(s) 707
AoxI GGCC 1 cut(s) 869
ApeKI GCWGC 2 cut(s) 552, 737
ApoI RAATTY 3 cut(s) 169, 850, 1031
AspS9I GGNCC 2 cut(s) 418, 1055
AsuC2I CCSGG 1 cut(s) 963
AsuHPI GGTGA 2 cut(s) 68, 371
AvaII GGWCC 2 cut(s) 418, 1055
BarI GAAGNNNNNNTAC 2 cut(s) 75, 107
BbsI GAAGAC 2 cut(s) 18, 1086
BbvI GCAGC 2 cut(s) 539, 724
BccI CCATC 4 cut(s) 528, 751, 830, 988
BciT130I CCWGG 2 cut(s) 252, 1053
BciVI GTATCC 1 cut(s) 467
BclI TGATCA 1 cut(s) 877
BcnI CCSGG 1 cut(s) 963
BcoDI GTCTC 3 cut(s) 191, 852, 905
BfaI CTAG 1 cut(s) 855
BfmI CTRYAG 1 cut(s) 915
BfuAI ACCTGC 1 cut(s) 589
BfuI GTATCC 1 cut(s) 467
BisI GCNGC 2 cut(s) 553, 738
BlsI GCNGC 2 cut(s) 554, 739
BmcAI AGTACT 1 cut(s) 483
Bme1390I CCNGG 3 cut(s) 252, 963, 1053
Bme18I GGWCC 2 cut(s) 418, 1055
BmgBI CACGTC 1 cut(s) 771
BmgT120I GGNCC 2 cut(s) 418, 1055
BmiI GGNNCC 1 cut(s) 420
BmrFI CCNGG 3 cut(s) 252, 963, 1053
BmrI ACTGGG 1 cut(s) 473
BmsI GCATC 3 cut(s) 379, 458, 489
BmuI ACTGGG 1 cut(s) 473
BpiI GAAGAC 2 cut(s) 18, 1086
BplI GAGNNNNNCTC 2 cut(s) 849, 881
BpmI CTGGAG 1 cut(s) 173
BpuEI CTTGAG 3 cut(s) 103, 386, 492
BpuMI CCSGG 1 cut(s) 963
BsaHI GRCGYC 1 cut(s) 713
BsaI GGTCTC 1 cut(s) 852
BsaJI CCNNGG 5 cut(s) 52, 250, 468, 639, 887
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 2 cut(s) 240, 674
Bse1I ACTGG 4 cut(s) 190, 285, 479, 692
BseBI CCWGG 2 cut(s) 252, 1053
BseDI CCNNGG 5 cut(s) 52, 250, 468, 639, 887
BseGI GGATG 4 cut(s) 439, 490, 514, 762
BseLI CCNNNNNNNGG 2 cut(s) 240, 674
BseMII CTCAG 4 cut(s) 509, 624, 880, 1082
BseNI ACTGG 4 cut(s) 190, 285, 479, 692
BseRI GAGGAG 1 cut(s) 855
BseXI GCAGC 2 cut(s) 539, 724
BsgI GTGCAG 1 cut(s) 561
BshFI GGCC 1 cut(s) 871
BsiSI CCGG 1 cut(s) 963
BslFI GGGAC 3 cut(s) 34, 404, 557
BslI CCNNNNNNNGG 2 cut(s) 240, 674
BsmAI GTCTC 3 cut(s) 191, 852, 905
BsmFI GGGAC 3 cut(s) 34, 404, 557
BsnI GGCC 1 cut(s) 871
Bso31I GGTCTC 1 cut(s) 852
Bsp1407I TGTACA 2 cut(s) 195, 397
Bsp143I GATC 4 cut(s) 526, 603, 830, 877
Bsp19I CCATGG 2 cut(s) 468, 639
BspACI CCGC 1 cut(s) 720
BspANI GGCC 1 cut(s) 871
BspCNI CTCAG 4 cut(s) 508, 623, 879, 1081
BspLI GGNNCC 1 cut(s) 420
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 589
BspPI GGATC 3 cut(s) 521, 611, 838
BspTNI GGTCTC 1 cut(s) 852
BsrGI TGTACA 2 cut(s) 195, 397
BsrI ACTGG 4 cut(s) 190, 285, 479, 692
BssECI CCNNGG 5 cut(s) 52, 250, 468, 639, 887
BssMI GATC 4 cut(s) 526, 603, 830, 877
BssNI GRCGYC 1 cut(s) 713
BssT1I CCWWGG 4 cut(s) 52, 468, 639, 887
Bst2UI CCWGG 2 cut(s) 252, 1053
Bst4CI ACNGT 2 cut(s) 131, 434
BstACI GRCGYC 1 cut(s) 713
BstAUI TGTACA 2 cut(s) 195, 397
BstC8I GCNNGC 1 cut(s) 504
BstDEI CTNAG 4 cut(s) 495, 610, 866, 1068
BstDSI CCRYGG 2 cut(s) 468, 639
BstF5I GGATG 4 cut(s) 439, 490, 514, 762
BstKTI GATC 4 cut(s) 529, 606, 833, 880
BstMAI GTCTC 3 cut(s) 191, 852, 905
BstMBI GATC 4 cut(s) 526, 603, 830, 877
BstMWI GCNNNNNNNGC 1 cut(s) 710
BstNI CCWGG 2 cut(s) 252, 1053
BstNSI RCATGY 3 cut(s) 466, 506, 751
BstSCI CCNGG 3 cut(s) 250, 961, 1051
BstSFI CTRYAG 1 cut(s) 915
BstV1I GCAGC 2 cut(s) 539, 724
BstV2I GAAGAC 2 cut(s) 18, 1086
BsuI GTATCC 1 cut(s) 467
BsuRI GGCC 1 cut(s) 871
BtgI CCRYGG 2 cut(s) 468, 639
BtrI CACGTC 1 cut(s) 771
BtsCI GGATG 4 cut(s) 439, 490, 514, 762
BtsI GCAGTG 1 cut(s) 912
BtsIMutI CAGTG 1 cut(s) 912
BveI ACCTGC 1 cut(s) 589
Cac8I GCNNGC 1 cut(s) 504
CaiI CAGNNNCTG 1 cut(s) 707
Cfr13I GGNCC 2 cut(s) 418, 1055
CseI GACGC 1 cut(s) 702
Csp6I GTAC 3 cut(s) 196, 398, 482
CviAII CATG 9 cut(s) 44, 199, 463, 469, 503, 557, 640, 748, 1062
CviJI RGCY 9 cut(s) 86, 537, 552, 644, 704, 776, 845, 871, 947
CviKI_1 RGCY 9 cut(s) 86, 537, 552, 644, 704, 776, 845, 871, 947
CviQI GTAC 3 cut(s) 196, 398, 482
DdeI CTNAG 4 cut(s) 495, 610, 866, 1068
DpnI GATC 4 cut(s) 528, 605, 832, 879
DpnII GATC 4 cut(s) 526, 603, 830, 877
Eco130I CCWWGG 4 cut(s) 52, 468, 639, 887
Eco31I GGTCTC 1 cut(s) 852
Eco47I GGWCC 2 cut(s) 418, 1055
Eco57I CTGAAG 3 cut(s) 353, 726, 795
EcoO109I RGGNCCY 1 cut(s) 1055
EcoRI GAATTC 1 cut(s) 1031
EcoRII CCWGG 2 cut(s) 250, 1051
EcoT14I CCWWGG 4 cut(s) 52, 468, 639, 887
EcoT22I ATGCAT 1 cut(s) 504
ErhI CCWWGG 4 cut(s) 52, 468, 639, 887
FaeI CATG 9 cut(s) 47, 202, 466, 472, 506, 560, 643, 751, 1065
FalI AAGNNNNNCTT 4 cut(s) 212, 244, 688, 720
FaqI GGGAC 3 cut(s) 34, 404, 557
FatI CATG 9 cut(s) 43, 198, 462, 468, 502, 556, 639, 747, 1061
FbaI TGATCA 1 cut(s) 877
Fnu4HI GCNGC 2 cut(s) 553, 738
FokI GGATG 4 cut(s) 426, 477, 501, 769
Fsp4HI GCNGC 2 cut(s) 553, 738
FspBI CTAG 1 cut(s) 855
GluI GCNGC 2 cut(s) 553, 738
GsuI CTGGAG 1 cut(s) 173
HaeIII GGCC 1 cut(s) 871
HapII CCGG 1 cut(s) 963
HgaI GACGC 1 cut(s) 702
Hin1I GRCGYC 1 cut(s) 713
Hin1II CATG 9 cut(s) 47, 202, 466, 472, 506, 560, 643, 751, 1065
HinfI GANTC 9 cut(s) 47, 98, 570, 661, 782, 986, 1004, 1043, 1048
HpaII CCGG 1 cut(s) 963
HphI GGTGA 2 cut(s) 68, 371
Hpy166II GTNNAC 2 cut(s) 59, 457
Hpy188III TCNNGA 7 cut(s) 144, 229, 385, 524, 779, 855, 1010
Hpy8I GTNNAC 2 cut(s) 59, 457
HpyCH4III ACNGT 2 cut(s) 131, 434
HpyCH4IV ACGT 1 cut(s) 770
HpyCH4V TGCA 6 cut(s) 22, 239, 427, 502, 578, 917
HpyF10VI GCNNNNNNNGC 1 cut(s) 710
HpyF3I CTNAG 4 cut(s) 495, 610, 866, 1068
HpySE526I ACGT 1 cut(s) 770
Hsp92I GRCGYC 1 cut(s) 713
Hsp92II CATG 9 cut(s) 47, 202, 466, 472, 506, 560, 643, 751, 1065
Ksp22I TGATCA 1 cut(s) 877
Kzo9I GATC 4 cut(s) 526, 603, 830, 877
LmnI GCTCC 2 cut(s) 91, 842
Lsp1109I GCAGC 2 cut(s) 539, 724
LweI GCATC 3 cut(s) 379, 458, 489
MaeI CTAG 1 cut(s) 855
MaeII ACGT 1 cut(s) 770
MaeIII GTNAC 1 cut(s) 819
MalI GATC 4 cut(s) 528, 605, 832, 879
MboI GATC 4 cut(s) 526, 603, 830, 877
MboII GAAGA 7 cut(s) 16, 18, 296, 299, 346, 1088, 1091
MluCI AATT 5 cut(s) 70, 169, 586, 850, 1031
MlyI GAGTC 4 cut(s) 56, 579, 776, 998
MmeI TCCRAC 3 cut(s) 616, 663, 784
Mph1103I ATGCAT 1 cut(s) 504
MseI TTAA 3 cut(s) 69, 564, 1090
MslI CAYNNNNRTG 2 cut(s) 268, 467
MspI CCGG 1 cut(s) 963
MspR9I CCNGG 3 cut(s) 252, 963, 1053
MvaI CCWGG 2 cut(s) 252, 1053
MwoI GCNNNNNNNGC 1 cut(s) 710
NciI CCSGG 1 cut(s) 963
NcoI CCATGG 2 cut(s) 468, 639
NdeII GATC 4 cut(s) 526, 603, 830, 877
NlaIII CATG 9 cut(s) 47, 202, 466, 472, 506, 560, 643, 751, 1065
NlaIV GGNNCC 1 cut(s) 420
NsiI ATGCAT 1 cut(s) 504
NspI RCATGY 3 cut(s) 466, 506, 751
PaeI GCATGC 1 cut(s) 506
PaqCI CACCTGC 1 cut(s) 589
PciI ACATGT 1 cut(s) 747
PfeI GAWTC 5 cut(s) 98, 661, 986, 1043, 1048
PflMI CCANNNNNTGG 1 cut(s) 674
PfoI TCCNGGA 1 cut(s) 1051
PkrI GCNGC 2 cut(s) 554, 739
PleI GAGTC 4 cut(s) 55, 578, 776, 998
PpsI GAGTC 4 cut(s) 55, 578, 776, 998
PpuMI RGGWCCY 1 cut(s) 1055
PscI ACATGT 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 1055
Psp6I CCWGG 2 cut(s) 250, 1051
PspGI CCWGG 2 cut(s) 250, 1051
PspN4I GGNNCC 1 cut(s) 420
PspPI GGNCC 2 cut(s) 418, 1055
PspPPI RGGWCCY 1 cut(s) 1055
PstI CTGCAG 1 cut(s) 919
PstNI CAGNNNCTG 1 cut(s) 707
RsaI GTAC 3 cut(s) 197, 399, 483
RsaNI GTAC 3 cut(s) 196, 398, 482
RseI CAYNNNNRTG 2 cut(s) 268, 467
SaqAI TTAA 3 cut(s) 69, 564, 1090
SatI GCNGC 2 cut(s) 553, 738
Sau3AI GATC 4 cut(s) 526, 603, 830, 877
Sau96I GGNCC 2 cut(s) 418, 1055
ScaI AGTACT 1 cut(s) 483
SchI GAGTC 4 cut(s) 56, 579, 776, 998
ScrFI CCNGG 3 cut(s) 252, 963, 1053
SfaNI GCATC 3 cut(s) 379, 458, 489
SfcI CTRYAG 1 cut(s) 915
SinI GGWCC 2 cut(s) 418, 1055
SmiMI CAYNNNNRTG 2 cut(s) 268, 467
SmlI CTYRAG 3 cut(s) 118, 365, 507
SmoI CTYRAG 3 cut(s) 118, 365, 507
SphI GCATGC 1 cut(s) 506
Sse9I AATT 5 cut(s) 70, 169, 586, 850, 1031
SsiI CCGC 1 cut(s) 720
SspMI CTAG 1 cut(s) 855
StyD4I CCNGG 3 cut(s) 250, 961, 1051
StyI CCWWGG 4 cut(s) 52, 468, 639, 887
TaaI ACNGT 2 cut(s) 131, 434
TaiI ACGT 1 cut(s) 773
TaqI TCGA 1 cut(s) 1046
TasI AATT 5 cut(s) 70, 169, 586, 850, 1031
TatI WGTACW 3 cut(s) 195, 397, 481
TfiI GAWTC 5 cut(s) 98, 661, 986, 1043, 1048
Tru1I TTAA 3 cut(s) 69, 564, 1090
Tru9I TTAA 3 cut(s) 69, 564, 1090
TscAI CASTG 1 cut(s) 919
TseI GCWGC 2 cut(s) 552, 737
TspDTI ATGAA 3 cut(s) 17, 428, 1044
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 1 cut(s) 919
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 2 cut(s) 418, 1055
XapI RAATTY 3 cut(s) 169, 850, 1031
XbaI TCTAGA 1 cut(s) 854
XceI RCATGY 3 cut(s) 466, 506, 751
XcmI CCANNNNNNNNNTGG 1 cut(s) 563
XspI CTAG 1 cut(s) 855
ZrmI AGTACT 1 cut(s) 483
Zsp2I ATGCAT 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.