AT3G09800

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002186
Physical Location & Seq
Reverse (-)
3006428 .. 3008610
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G09800.2

Sequence Viewer

Length: 540 bp
ATGTCTCCTGATTCTTGTCCTTTGGTAAAGAAGATTCTTCTTCTAGACTCTGAAGGAAAGCGTGTGGCTGTCAAGTATTACTCGGATGACTGGCCAACTAATGCCGCCAAGTTAAGTTTTGAGAAGTATGTATTCTCAAAGACCTCTAAGACCAATGCTCGTACAGAAGCTGAGATCACACTGTTGGACAGCAATATTATTGTCTATAAGTTTGCCCAGGACCTTCACTTCTTTGTTACTGGAGGTGAAAATGAAAACGAGCTCATCTTAGCTTCTGTTCTTCAAGGCTTTTTTGATGCTGTTGCACTTCTTCTGAGGAGCAATGTGGAAAAGATGGAAGCCCTTGAGAACTTGGATCTCATCTTTCTATGCCTTGATGAAATGGTCGATCAAGGGGTGGTTCTTGAAACGGATCCAAACGTCATTGCGGGGAAAGTAGCAATGCAGAGCACAGAAGCTAGTGGTTCACTCTCTGAACAGACACTAACACAAGCGCTAGCAACAGCTCGGGAGCATCTGGCAAGAAGTCTGCTTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

179

Amino Acids

19.75

Weight (kDa)

4.65

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 19 - 111 9.3e-16 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 105, 428
AclWI GGATC 3 cut(s) 363, 407, 420
AcoI YGGCCR 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 72
AfaI GTAC 1 cut(s) 163
AfeI AGCGCT 1 cut(s) 495
AgsI TTSAA 2 cut(s) 284, 407
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 5 cut(s) 170, 262, 272, 458, 506
AluI AGCT 5 cut(s) 170, 262, 272, 458, 506
Alw21I GWGCWC 2 cut(s) 264, 452
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 3 cut(s) 363, 407, 420
AlwNI CAGNNNCTG 1 cut(s) 170
Ama87I CYCGRG 1 cut(s) 507
Aor51HI AGCGCT 1 cut(s) 495
AoxI GGCC 1 cut(s) 92
AspLEI GCGC 1 cut(s) 496
AspS9I GGNCC 1 cut(s) 220
AsuHPI GGTGA 1 cut(s) 257
AsuNHI GCTAGC 1 cut(s) 496
AvaI CYCGRG 1 cut(s) 507
AvaII GGWCC 1 cut(s) 220
BalI TGGCCA 1 cut(s) 94
BamHI GGATCC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 264
BarI GAAGNNNNNNTAC 1 cut(s) 517
Bbv12I GWGCWC 2 cut(s) 264, 452
BccI CCATC 1 cut(s) 328
BciT130I CCWGG 1 cut(s) 218
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 44, 459, 497
BfoI RGCGCY 1 cut(s) 497
BisI GCNGC 1 cut(s) 105
BlsI GCNGC 1 cut(s) 106
Bme1390I CCNGG 1 cut(s) 218
Bme18I GGWCC 1 cut(s) 220
BmeT110I CYCGRG 1 cut(s) 507
BmgT120I GGNCC 1 cut(s) 220
BmiI GGNNCC 1 cut(s) 414
BmrFI CCNGG 1 cut(s) 218
BmsI GCATC 2 cut(s) 286, 523
BmtI GCTAGC 1 cut(s) 500
BpmI CTGGAG 1 cut(s) 261
BpuEI CTTGAG 1 cut(s) 365
BsaJI CCNNGG 1 cut(s) 216
Bse1I ACTGG 2 cut(s) 95, 244
Bse3DI GCAATG 3 cut(s) 328, 423, 447
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 1 cut(s) 91
BseMI GCAATG 3 cut(s) 328, 423, 447
BseMII CTCAG 2 cut(s) 162, 305
BseNI ACTGG 2 cut(s) 95, 244
BseRI GAGGAG 1 cut(s) 331
BshFI GGCC 1 cut(s) 94
BsiHKAI GWGCWC 2 cut(s) 264, 452
BsiHKCI CYCGRG 1 cut(s) 507
BsmAI GTCTC 1 cut(s) 9
BsnI GGCC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 507
Bsp1286I GDGCHC 2 cut(s) 264, 452
Bsp143I GATC 4 cut(s) 174, 355, 388, 412
BspACI CCGC 2 cut(s) 105, 428
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 2 cut(s) 163, 306
BspLI GGNNCC 1 cut(s) 414
BspOI GCTAGC 1 cut(s) 500
BspPI GGATC 3 cut(s) 363, 407, 420
BsrDI GCAATG 3 cut(s) 328, 423, 447
BsrI ACTGG 2 cut(s) 95, 244
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 4 cut(s) 174, 355, 388, 412
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 4 cut(s) 147, 171, 268, 314
BstF5I GGATG 1 cut(s) 91
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 1 cut(s) 496
BstKTI GATC 4 cut(s) 177, 358, 391, 415
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 174, 355, 388, 412
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 1 cut(s) 216
BstX2I RGATCY 2 cut(s) 355, 412
BstYI RGATCY 2 cut(s) 355, 412
BsuRI GGCC 1 cut(s) 94
BtsCI GGATG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 498
CaiI CAGNNNCTG 1 cut(s) 170
CfoI GCGC 1 cut(s) 496
Cfr13I GGNCC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 162
CviAII CATG 1 cut(s) 537
CviJI RGCY 9 cut(s) 68, 94, 170, 262, 272, 288, 341, 458, 506
CviKI_1 RGCY 9 cut(s) 68, 94, 170, 262, 272, 288, 341, 458, 506
CviQI GTAC 1 cut(s) 162
DdeI CTNAG 4 cut(s) 147, 171, 268, 314
DpnI GATC 4 cut(s) 176, 357, 390, 414
DpnII GATC 4 cut(s) 174, 355, 388, 412
EaeI YGGCCR 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 220
Eco47III AGCGCT 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 72
Eco88I CYCGRG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 262
EcoO109I RGGNCCY 1 cut(s) 220
EcoRII CCWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 264
FaeI CATG 1 cut(s) 540
FaiI YATR 4 cut(s) 129, 207, 370, 538
FatI CATG 1 cut(s) 536
FauI CCCGC 1 cut(s) 421
Fnu4HI GCNGC 1 cut(s) 105
FokI GGATG 1 cut(s) 98
FriOI GRGCYC 1 cut(s) 264
Fsp4HI GCNGC 1 cut(s) 105
FspBI CTAG 3 cut(s) 44, 459, 497
GlaI GCGC 1 cut(s) 495
GluI GCNGC 1 cut(s) 105
GsuI CTGGAG 1 cut(s) 261
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 1 cut(s) 94
HhaI GCGC 1 cut(s) 496
Hin1II CATG 1 cut(s) 540
Hin6I GCGC 1 cut(s) 494
HinP1I GCGC 1 cut(s) 494
HinfI GANTC 3 cut(s) 11, 34, 47
HphI GGTGA 1 cut(s) 257
Hpy166II GTNNAC 1 cut(s) 467
Hpy188I TCNGA 4 cut(s) 52, 85, 315, 475
Hpy188III TCNNGA 4 cut(s) 8, 44, 404, 509
Hpy8I GTNNAC 1 cut(s) 467
HpyAV CCTTC 2 cut(s) 47, 233
HpyCH4III ACNGT 1 cut(s) 183
HpyCH4IV ACGT 1 cut(s) 420
HpyCH4V TGCA 2 cut(s) 305, 445
HpyF3I CTNAG 4 cut(s) 147, 171, 268, 314
HpySE526I ACGT 1 cut(s) 420
Hsp92II CATG 1 cut(s) 540
HspAI GCGC 1 cut(s) 494
Kzo9I GATC 4 cut(s) 174, 355, 388, 412
LmnI GCTCC 2 cut(s) 318, 511
LpnPI CCDG 6 cut(s) 21, 76, 203, 225, 230, 503
LweI GCATC 2 cut(s) 286, 523
MaeI CTAG 3 cut(s) 44, 459, 497
MaeII ACGT 1 cut(s) 420
MaeIII GTNAC 1 cut(s) 235
MalI GATC 4 cut(s) 176, 357, 390, 414
MboI GATC 4 cut(s) 174, 355, 388, 412
MboII GAAGA 5 cut(s) 29, 32, 43, 272, 302
MflI RGATCY 2 cut(s) 355, 412
MhlI GDGCHC 2 cut(s) 264, 452
MlsI TGGCCA 1 cut(s) 94
MluNI TGGCCA 1 cut(s) 94
MlyI GAGTC 1 cut(s) 41
MmeI TCCRAC 1 cut(s) 165
MnlI CCTC 3 cut(s) 154, 236, 309
Mox20I TGGCCA 1 cut(s) 94
MscI TGGCCA 1 cut(s) 94
MseI TTAA 1 cut(s) 113
Msp20I TGGCCA 1 cut(s) 94
MspR9I CCNGG 1 cut(s) 218
MvaI CCWGG 1 cut(s) 218
NdeII GATC 4 cut(s) 174, 355, 388, 412
NheI GCTAGC 1 cut(s) 496
NlaIII CATG 1 cut(s) 540
NlaIV GGNNCC 1 cut(s) 414
PfeI GAWTC 2 cut(s) 11, 34
PkrI GCNGC 1 cut(s) 106
PleI GAGTC 1 cut(s) 41
PpsI GAGTC 1 cut(s) 41
PpuMI RGGWCCY 1 cut(s) 220
Psp124BI GAGCTC 1 cut(s) 264
Psp5II RGGWCCY 1 cut(s) 220
Psp6I CCWGG 1 cut(s) 216
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 220
PspPPI RGGWCCY 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 170
PsuI RGATCY 2 cut(s) 355, 412
RsaI GTAC 1 cut(s) 163
RsaNI GTAC 1 cut(s) 162
SacI GAGCTC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 113
SatI GCNGC 1 cut(s) 105
Sau3AI GATC 4 cut(s) 174, 355, 388, 412
Sau96I GGNCC 1 cut(s) 220
SchI GAGTC 1 cut(s) 41
ScrFI CCNGG 1 cut(s) 218
SduI GDGCHC 2 cut(s) 264, 452
SetI ASST 9 cut(s) 146, 172, 225, 247, 264, 274, 423, 460, 508
SfaNI GCATC 2 cut(s) 286, 523
SinI GGWCC 1 cut(s) 220
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
SsiI CCGC 2 cut(s) 105, 428
SspI AATATT 1 cut(s) 196
SspMI CTAG 3 cut(s) 44, 459, 497
SstI GAGCTC 1 cut(s) 264
StyD4I CCNGG 1 cut(s) 216
TaaI ACNGT 1 cut(s) 183
TaiI ACGT 1 cut(s) 423
TaqI TCGA 1 cut(s) 387
TauI GCSGC 1 cut(s) 107
TfiI GAWTC 2 cut(s) 11, 34
Tru1I TTAA 1 cut(s) 113
Tru9I TTAA 1 cut(s) 113
TscAI CASTG 1 cut(s) 186
TspDTI ATGAA 2 cut(s) 267, 393
TspGWI ACGGA 1 cut(s) 425
TspRI CASTG 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 220
XbaI TCTAGA 1 cut(s) 43
XspI CTAG 3 cut(s) 44, 459, 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.