Rroxscaffold_1G00063480

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002186
Physical Location & Seq
Forward (+)
85360930 .. 85362264
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00063480.1

Sequence Viewer

Length: 528 bp
ATGGGAAGTCTCTTCAATTATCGTGATTCATCCCCATCTGTGAAGAACATTCTTCTTCTAGACTCTGAAGGGAAGCGTGTGGCTGTCAAGTATTACTCAGATGATTGGCCAACAAATGGCGCAAAGCTAGCTTTTGAAAAATCTGTATTCGCTAAGACTCTAAAGACAAATGCAAGGCTAGAAGCGGAGATAATGATGTTCGACAGCAACGTTGTTATATACAAGTTTATGCAGGACCTTCACTTCTTTGTGACTGGAGGCGACGATGAAAACGAACTCATTTTAGCCACTGTACTTCAGGGATTCTTTGATGCAGTTGCCTTTCTCTTGAGGCACAATGTCGACAAAAGGGAGGCGCTAGAGAACCTGGATCTCATACTTCTATGCTTTGATGAGATCGTTGATGGAGGGATGATACTTGAAACAGATCCGACTGTTATCGCAGGAAAGGTAGCAACTCATACCATGGATGCTGATGCACCATTGTCTGAGCAGGCGCTCAAAAGTGCTTCTGGAGGCATTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

175

Amino Acids

19.36

Weight (kDa)

4.6

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 14 - 153 1.9e-21 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 116
AccI GTMKAC 1 cut(s) 342
AciI CCGC 1 cut(s) 185
AclI AACGTT 1 cut(s) 210
AclWI GGATC 2 cut(s) 378, 422
AcoI YGGCCR 1 cut(s) 107
AcuI CTGAAG 2 cut(s) 87, 281
AfaI GTAC 1 cut(s) 294
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 3 cut(s) 16, 137, 422
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 2 cut(s) 127, 131
AluI AGCT 2 cut(s) 127, 131
Alw26I GTCTC 1 cut(s) 14
AlwI GGATC 2 cut(s) 378, 422
AoxI GGCC 1 cut(s) 107
AspLEI GCGC 3 cut(s) 122, 358, 499
AspS9I GGNCC 1 cut(s) 235
AsuNHI GCTAGC 1 cut(s) 127
AvaII GGWCC 1 cut(s) 235
BalI TGGCCA 1 cut(s) 109
BccI CCATC 2 cut(s) 43, 398
BciT130I CCWGG 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 14
BfaI CTAG 4 cut(s) 59, 128, 179, 359
BfoI RGCGCY 2 cut(s) 359, 500
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 368
BmsI GCATC 3 cut(s) 301, 460, 466
BmtI GCTAGC 1 cut(s) 131
BpmI CTGGAG 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 465
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 3 cut(s) 29, 417, 475
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 2 cut(s) 111, 480
BseNI ACTGG 1 cut(s) 259
BshFI GGCC 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 14
BsmI GAATGC 1 cut(s) 519
BsnI GGCC 1 cut(s) 109
Bsp143I GATC 3 cut(s) 370, 396, 427
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 1 cut(s) 185
BspANI GGCC 1 cut(s) 109
BspCNI CTCAG 2 cut(s) 110, 481
BspOI GCTAGC 1 cut(s) 131
BspPI GGATC 2 cut(s) 378, 422
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 3 cut(s) 370, 396, 427
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 2 cut(s) 292, 436
Bst6I CTCTTC 1 cut(s) 17
BstC8I GCNNGC 2 cut(s) 129, 495
BstDEI CTNAG 3 cut(s) 97, 153, 489
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 3 cut(s) 29, 417, 475
BstH2I RGCGCY 2 cut(s) 359, 500
BstHHI GCGC 3 cut(s) 122, 358, 499
BstKTI GATC 3 cut(s) 373, 399, 430
BstMAI GTCTC 1 cut(s) 14
BstMBI GATC 3 cut(s) 370, 396, 427
BstMWI GCNNNNNNNGC 1 cut(s) 128
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 366
BstX2I RGATCY 2 cut(s) 370, 427
BstYI RGATCY 2 cut(s) 370, 427
BsuRI GGCC 1 cut(s) 109
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 3 cut(s) 29, 417, 475
BtsIMutI CAGTG 1 cut(s) 288
Cac8I GCNNGC 2 cut(s) 129, 495
CfoI GCGC 3 cut(s) 122, 358, 499
Cfr13I GGNCC 1 cut(s) 235
Csp6I GTAC 1 cut(s) 293
CviAII CATG 1 cut(s) 466
CviJI RGCY 6 cut(s) 83, 109, 127, 131, 178, 287
CviKI_1 RGCY 6 cut(s) 83, 109, 127, 131, 178, 287
CviQI GTAC 1 cut(s) 293
DdeI CTNAG 3 cut(s) 97, 153, 489
DpnI GATC 3 cut(s) 372, 398, 429
DpnII GATC 3 cut(s) 370, 396, 427
EaeI YGGCCR 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 87, 281
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 1 cut(s) 469
FaiI YATR 7 cut(s) 218, 220, 230, 377, 385, 462, 467
FatI CATG 1 cut(s) 465
FblI GTMKAC 1 cut(s) 342
FokI GGATG 3 cut(s) 16, 424, 482
FspBI CTAG 4 cut(s) 59, 128, 179, 359
GlaI GCGC 3 cut(s) 121, 357, 498
GsuI CTGGAG 1 cut(s) 276
HaeII RGCGCY 2 cut(s) 359, 500
HaeIII GGCC 1 cut(s) 109
HhaI GCGC 3 cut(s) 122, 358, 499
Hin1II CATG 1 cut(s) 469
Hin6I GCGC 3 cut(s) 120, 356, 497
HinP1I GCGC 3 cut(s) 120, 356, 497
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 4 cut(s) 26, 62, 157, 303
Hpy166II GTNNAC 1 cut(s) 343
Hpy188I TCNGA 4 cut(s) 67, 100, 432, 490
Hpy188III TCNNGA 4 cut(s) 23, 59, 328, 513
Hpy8I GTNNAC 1 cut(s) 343
Hpy99I CGWCG 1 cut(s) 266
HpyAV CCTTC 2 cut(s) 62, 248
HpyCH4III ACNGT 2 cut(s) 292, 436
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 4 cut(s) 173, 232, 314, 479
HpyF10VI GCNNNNNNNGC 1 cut(s) 128
HpyF3I CTNAG 3 cut(s) 97, 153, 489
HpySE526I ACGT 1 cut(s) 210
Hsp92II CATG 1 cut(s) 469
HspAI GCGC 3 cut(s) 120, 356, 497
Kzo9I GATC 3 cut(s) 370, 396, 427
LpnPI CCDG 8 cut(s) 218, 240, 284, 353, 380, 429, 479, 498
LweI GCATC 3 cut(s) 301, 460, 466
MaeI CTAG 4 cut(s) 59, 128, 179, 359
MaeII ACGT 1 cut(s) 210
MaeIII GTNAC 1 cut(s) 250
MalI GATC 3 cut(s) 372, 398, 429
MboI GATC 3 cut(s) 370, 396, 427
MboII GAAGA 4 cut(s) 4, 44, 47, 55
MflI RGATCY 2 cut(s) 370, 427
MlsI TGGCCA 1 cut(s) 109
MluCI AATT 1 cut(s) 16
MluNI TGGCCA 1 cut(s) 109
MlyI GAGTC 2 cut(s) 56, 151
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 5 cut(s) 251, 324, 346, 401, 509
Mox20I TGGCCA 1 cut(s) 109
MscI TGGCCA 1 cut(s) 109
Msp20I TGGCCA 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 368
Mva1269I GAATGC 1 cut(s) 519
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 1 cut(s) 128
NcoI CCATGG 1 cut(s) 465
NdeII GATC 3 cut(s) 370, 396, 427
NheI GCTAGC 1 cut(s) 127
NlaIII CATG 1 cut(s) 469
NmuCI GTSAC 1 cut(s) 250
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 270
PctI GAATGC 1 cut(s) 519
PfeI GAWTC 2 cut(s) 26, 303
PflMI CCANNNNNTGG 1 cut(s) 116
PleI GAGTC 2 cut(s) 56, 151
PpsI GAGTC 2 cut(s) 56, 151
PpuMI RGGWCCY 1 cut(s) 235
Psp1406I AACGTT 1 cut(s) 210
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 2 cut(s) 370, 427
RsaI GTAC 1 cut(s) 294
RsaNI GTAC 1 cut(s) 293
SalI GTCGAC 1 cut(s) 341
Sau3AI GATC 3 cut(s) 370, 396, 427
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 56, 151
ScrFI CCNGG 1 cut(s) 368
SetI ASST 6 cut(s) 129, 133, 213, 240, 369, 453
SfaNI GCATC 3 cut(s) 301, 460, 466
SinI GGWCC 1 cut(s) 235
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
Sse9I AATT 1 cut(s) 16
SsiI CCGC 1 cut(s) 185
SspMI CTAG 4 cut(s) 59, 128, 179, 359
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 2 cut(s) 292, 436
TaiI ACGT 1 cut(s) 213
TaqI TCGA 2 cut(s) 201, 342
TasI AATT 1 cut(s) 16
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 2 cut(s) 26, 303
TscAI CASTG 1 cut(s) 295
TseFI GTSAC 1 cut(s) 250
Tsp45I GTSAC 1 cut(s) 250
TspDTI ATGAA 2 cut(s) 18, 282
TspRI CASTG 1 cut(s) 295
Van91I CCANNNNNTGG 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 235
XbaI TCTAGA 1 cut(s) 58
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 4 cut(s) 59, 128, 179, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.