MD09G1190300.v1.1

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002186
Physical Location & Seq
Reverse (-)
16639568 .. 16642275
2708 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1190300.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGCAAGCCTCTTCAGCTACCGTGACTCGTGCCCTTCTGTGAAGAATATTCTTCTTCTGGACTCTGAAGGAAAGCGTGTGGCTGTCAAGTACTTCTCAGATGATTGGCCAACAAATGGTGCAAAGTTGGCTTTTGAAAAATCTGTGTTCACTAAGACTCTGAAGACAAATGCTCGGATAGAAGCGGAGATAATGATGTTTGAGAGCAACGTTGTTATATATAAGTTCGTGCAGGACCTTCACTTTTTTGTGACTGGAGGTGACGATGAAAATGAACTGATTTTAGCCACAGTTCTCCAGGGATTTTTTGATGCAGTTGCCCTTCTCTTGAGGAACAACGTTGACAAACGTGAGGCACTAGAGAATCTGGATCTTATACTTTTATGTTTCGATGAGATTGTTGATGGAGGGATGATTCTCGAAACAGATCCAAGCATTATTGCAGGAAAGGTGGCAACTCATACCATGGATGCAGATGCACCCTTGTCTGAGCAGACCATAACACAAGCATGGGCAACAGCACGGGAGCATCTAACCAGAACCCTTCTCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

185

Amino Acids

20.58

Weight (kDa)

4.73

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 14 - 153 6.1e-21 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 116
AciI CCGC 1 cut(s) 185
AclI AACGTT 2 cut(s) 210, 339
AclWI GGATC 2 cut(s) 378, 422
AcoI YGGCCR 1 cut(s) 107
AcuI CTGAAG 2 cut(s) 87, 182
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 1 cut(s) 137
AjnI CCWGG 1 cut(s) 297
AluBI AGCT 1 cut(s) 18
AluI AGCT 1 cut(s) 18
AlwI GGATC 2 cut(s) 378, 422
AoxI GGCC 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 235
AsuHPI GGTGA 1 cut(s) 272
AvaII GGWCC 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 35
BalI TGGCCA 1 cut(s) 109
BauI CACGAG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 170
BccI CCATC 1 cut(s) 398
BciT130I CCWGG 1 cut(s) 299
BfaI CTAG 1 cut(s) 359
BmcAI AGTACT 1 cut(s) 92
Bme1390I CCNGG 1 cut(s) 299
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 299
BmsI GCATC 4 cut(s) 301, 460, 466, 538
BpiI GAAGAC 1 cut(s) 170
BpmI CTGGAG 2 cut(s) 276, 281
BpuEI CTTGAG 1 cut(s) 349
BsaJI CCNNGG 2 cut(s) 298, 465
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 259
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 2 cut(s) 298, 465
BseGI GGATG 2 cut(s) 417, 475
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMII CTCAG 2 cut(s) 111, 480
BseNI ACTGG 1 cut(s) 259
BseSI GKGCMC 1 cut(s) 35
BsgI GTGCAG 1 cut(s) 251
BshFI GGCC 1 cut(s) 109
BslI CCNNNNNNNGG 1 cut(s) 116
BsnI GGCC 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 35
Bsp143I GATC 2 cut(s) 370, 427
Bsp19I CCATGG 1 cut(s) 465
BspACI CCGC 1 cut(s) 185
BspANI GGCC 1 cut(s) 109
BspCNI CTCAG 2 cut(s) 110, 481
BspPI GGATC 2 cut(s) 378, 422
BsrI ACTGG 1 cut(s) 259
BssECI CCNNGG 2 cut(s) 298, 465
BssMI GATC 2 cut(s) 370, 427
BssSI CACGAG 1 cut(s) 28
BssT1I CCWWGG 1 cut(s) 465
Bst2BI CACGAG 1 cut(s) 28
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 2 cut(s) 23, 292
Bst6I CTCTTC 1 cut(s) 17
BstC8I GCNNGC 1 cut(s) 7
BstDEI CTNAG 3 cut(s) 97, 153, 489
BstDSI CCRYGG 1 cut(s) 465
BstF5I GGATG 2 cut(s) 417, 475
BstKTI GATC 2 cut(s) 373, 430
BstMBI GATC 2 cut(s) 370, 427
BstMWI GCNNNNNNNGC 2 cut(s) 15, 128
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 297
BstSLI GKGCMC 1 cut(s) 35
BstV2I GAAGAC 1 cut(s) 170
BstX2I RGATCY 2 cut(s) 370, 427
BstYI RGATCY 2 cut(s) 370, 427
BsuRI GGCC 1 cut(s) 109
BtgI CCRYGG 1 cut(s) 465
BtsCI GGATG 2 cut(s) 417, 475
Cac8I GCNNGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 235
Csp6I GTAC 1 cut(s) 91
CviAII CATG 2 cut(s) 466, 510
CviJI RGCY 6 cut(s) 9, 18, 83, 109, 131, 287
CviKI_1 RGCY 6 cut(s) 9, 18, 83, 109, 131, 287
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 3 cut(s) 97, 153, 489
DpnI GATC 2 cut(s) 372, 429
DpnII GATC 2 cut(s) 370, 427
EaeI YGGCCR 1 cut(s) 107
Eam1104I CTCTTC 1 cut(s) 17
EarI CTCTTC 1 cut(s) 17
Eco130I CCWWGG 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 235
Eco57I CTGAAG 2 cut(s) 87, 182
EcoO109I RGGNCCY 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 297
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 2 cut(s) 469, 513
FaiI YATR 9 cut(s) 218, 220, 222, 377, 385, 462, 467, 500, 511
FatI CATG 2 cut(s) 465, 509
FokI GGATG 2 cut(s) 424, 482
FspBI CTAG 1 cut(s) 359
GsuI CTGGAG 2 cut(s) 276, 281
HaeIII GGCC 1 cut(s) 109
Hin1II CATG 2 cut(s) 469, 513
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 5 cut(s) 26, 62, 157, 364, 415
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 2 cut(s) 150, 343
Hpy188I TCNGA 5 cut(s) 67, 100, 162, 177, 490
Hpy188III TCNNGA 4 cut(s) 59, 328, 368, 419
Hpy8I GTNNAC 2 cut(s) 150, 343
HpyAV CCTTC 5 cut(s) 45, 62, 248, 332, 554
HpyCH4III ACNGT 2 cut(s) 23, 292
HpyCH4IV ACGT 3 cut(s) 210, 339, 349
HpyCH4V TGCA 6 cut(s) 122, 232, 314, 443, 473, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 15, 128
HpyF3I CTNAG 3 cut(s) 97, 153, 489
HpySE526I ACGT 3 cut(s) 210, 339, 349
Hsp92II CATG 2 cut(s) 469, 513
Kzo9I GATC 2 cut(s) 370, 427
LmnI GCTCC 1 cut(s) 526
LpnPI CCDG 8 cut(s) 44, 218, 240, 284, 311, 353, 429, 550
LweI GCATC 4 cut(s) 301, 460, 466, 538
MaeI CTAG 1 cut(s) 359
MaeII ACGT 3 cut(s) 210, 339, 349
MaeIII GTNAC 3 cut(s) 23, 250, 260
MalI GATC 2 cut(s) 372, 429
MboI GATC 2 cut(s) 370, 427
MboII GAAGA 5 cut(s) 4, 44, 47, 55, 175
MflI RGATCY 2 cut(s) 370, 427
MhlI GDGCHC 1 cut(s) 35
MlsI TGGCCA 1 cut(s) 109
MluNI TGGCCA 1 cut(s) 109
MlyI GAGTC 3 cut(s) 20, 56, 151
MnlI CCTC 5 cut(s) 20, 251, 324, 346, 401
Mox20I TGGCCA 1 cut(s) 109
MscI TGGCCA 1 cut(s) 109
MslI CAYNNNNRTG 1 cut(s) 508
Msp20I TGGCCA 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 299
MvaI CCWGG 1 cut(s) 299
MwoI GCNNNNNNNGC 2 cut(s) 15, 128
NcoI CCATGG 1 cut(s) 465
NdeII GATC 2 cut(s) 370, 427
NlaIII CATG 2 cut(s) 469, 513
NmuCI GTSAC 3 cut(s) 23, 250, 260
PfeI GAWTC 2 cut(s) 364, 415
PflMI CCANNNNNTGG 1 cut(s) 116
PleI GAGTC 3 cut(s) 20, 56, 151
PpsI GAGTC 3 cut(s) 20, 56, 151
PpuMI RGGWCCY 1 cut(s) 235
Psp1406I AACGTT 2 cut(s) 210, 339
Psp5II RGGWCCY 1 cut(s) 235
Psp6I CCWGG 1 cut(s) 297
PspGI CCWGG 1 cut(s) 297
PspPI GGNCC 1 cut(s) 235
PspPPI RGGWCCY 1 cut(s) 235
PsuI RGATCY 2 cut(s) 370, 427
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 508
Sau3AI GATC 2 cut(s) 370, 427
Sau96I GGNCC 1 cut(s) 235
ScaI AGTACT 1 cut(s) 92
SchI GAGTC 3 cut(s) 20, 56, 151
ScrFI CCNGG 1 cut(s) 299
SduI GDGCHC 1 cut(s) 35
SetI ASST 7 cut(s) 20, 213, 240, 262, 342, 352, 453
SfaNI GCATC 4 cut(s) 301, 460, 466, 538
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 1 cut(s) 508
SmlI CTYRAG 1 cut(s) 328
SmoI CTYRAG 1 cut(s) 328
SsiI CCGC 1 cut(s) 185
SspI AATATT 1 cut(s) 49
SspMI CTAG 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 297
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 2 cut(s) 23, 292
TaiI ACGT 3 cut(s) 213, 342, 352
TaqI TCGA 2 cut(s) 390, 420
TatI WGTACW 1 cut(s) 90
TfiI GAWTC 2 cut(s) 364, 415
TseFI GTSAC 3 cut(s) 23, 250, 260
Tsp45I GTSAC 3 cut(s) 23, 250, 260
TspDTI ATGAA 2 cut(s) 282, 288
Van91I CCANNNNNTGG 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 235
XspI CTAG 1 cut(s) 359
ZrmI AGTACT 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.