RchiOBHm_Chr2g0124081

Coatomer subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002186
Physical Location & Seq
Forward (+)
37427493 .. 37428469
977 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49631

Sequence Viewer

Length: 486 bp
ATGAAATTATTAGGCTACCTGCGTATAGGATTTGGAGTTGATGATTCATCCCCATCTGTGAATAACATTCTTCTTCTAGACTCTGAAGGGAAGCGTGTGGCTGTCAAGTATTACTCAGATGATTGGCCAACAAATGGTGCAAAGCTAGCTTTTGAAAAATCTGTATTCGCTAAGACTCTAAAGACCAATGCAAGGCTAGAAGCGGAGATAATGATGTTCGACAGCAACATTGTTATATACAAGTTTATGCAGGACCTTCACTTCTTTGTGACTGGAGGCGACGATGAAAACGAACTCATTTTAGCCACTGTACTTCAGGGATTCTTTGATGCAGTTGCCCTTCTCTTGAGGGAGGCGCTAGAGAACCTGGATCTCATACTTCTATGCTTTGATGAGATCGTAGATGGAGGGATGATACTTGAAACAGATCCAACTGTTATCGCAGGAAAGGTAGCAACTCATACCATTTGCTGGTTGTGCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

161

Amino Acids

17.97

Weight (kDa)

4.45

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 20 - 152 7.4e-17 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 27
AccB7I CCANNNNNTGG 2 cut(s) 134, 471
AciI CCGC 1 cut(s) 203
AclWI GGATC 2 cut(s) 378, 422
AcoI YGGCCR 1 cut(s) 125
AcuI CTGAAG 2 cut(s) 105, 299
AfaI GTAC 1 cut(s) 312
AfiI CCNNNNNNNGG 3 cut(s) 134, 192, 471
AgsI TTSAA 2 cut(s) 155, 422
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 2 cut(s) 145, 149
AluI AGCT 2 cut(s) 145, 149
AlwI GGATC 2 cut(s) 378, 422
AoxI GGCC 1 cut(s) 125
AspLEI GCGC 1 cut(s) 358
AspS9I GGNCC 1 cut(s) 253
AsuNHI GCTAGC 1 cut(s) 145
AvaII GGWCC 1 cut(s) 253
BalI TGGCCA 1 cut(s) 127
BccI CCATC 2 cut(s) 61, 398
BciT130I CCWGG 1 cut(s) 368
BfaI CTAG 4 cut(s) 77, 146, 197, 359
BfoI RGCGCY 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 27
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 253
BmgT120I GGNCC 1 cut(s) 253
BmrFI CCNGG 1 cut(s) 368
BmsI GCATC 1 cut(s) 319
BmtI GCTAGC 1 cut(s) 149
BpmI CTGGAG 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 367
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 192, 471
Bse1I ACTGG 1 cut(s) 277
BseBI CCWGG 1 cut(s) 368
BseGI GGATG 2 cut(s) 47, 417
BseLI CCNNNNNNNGG 3 cut(s) 134, 192, 471
BseMII CTCAG 1 cut(s) 129
BseNI ACTGG 1 cut(s) 277
BshFI GGCC 1 cut(s) 127
BslI CCNNNNNNNGG 3 cut(s) 134, 192, 471
BsnI GGCC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 370, 396, 427
BspACI CCGC 1 cut(s) 203
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 128
BspMI ACCTGC 1 cut(s) 27
BspOI GCTAGC 1 cut(s) 149
BspPI GGATC 2 cut(s) 378, 422
BsrI ACTGG 1 cut(s) 277
BssMI GATC 3 cut(s) 370, 396, 427
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 2 cut(s) 310, 436
BstC8I GCNNGC 1 cut(s) 147
BstDEI CTNAG 2 cut(s) 115, 171
BstF5I GGATG 2 cut(s) 47, 417
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 3 cut(s) 373, 399, 430
BstMBI GATC 3 cut(s) 370, 396, 427
BstMWI GCNNNNNNNGC 2 cut(s) 146, 477
BstNI CCWGG 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 366
BstX2I RGATCY 2 cut(s) 370, 427
BstYI RGATCY 2 cut(s) 370, 427
BsuRI GGCC 1 cut(s) 127
BtsCI GGATG 2 cut(s) 47, 417
BtsIMutI CAGTG 1 cut(s) 306
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 1 cut(s) 147
CfoI GCGC 1 cut(s) 358
Cfr13I GGNCC 1 cut(s) 253
Csp6I GTAC 1 cut(s) 311
CviJI RGCY 7 cut(s) 15, 101, 127, 145, 149, 196, 305
CviKI_1 RGCY 7 cut(s) 15, 101, 127, 145, 149, 196, 305
CviQI GTAC 1 cut(s) 311
DdeI CTNAG 2 cut(s) 115, 171
DpnI GATC 3 cut(s) 372, 398, 429
DpnII GATC 3 cut(s) 370, 396, 427
EaeI YGGCCR 1 cut(s) 125
Eco47I GGWCC 1 cut(s) 253
Eco57I CTGAAG 2 cut(s) 105, 299
EcoO109I RGGNCCY 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 366
FaiI YATR 7 cut(s) 26, 236, 238, 248, 377, 385, 462
FokI GGATG 2 cut(s) 34, 424
FspBI CTAG 4 cut(s) 77, 146, 197, 359
GlaI GCGC 1 cut(s) 357
GsuI CTGGAG 1 cut(s) 294
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 1 cut(s) 127
HhaI GCGC 1 cut(s) 358
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HinfI GANTC 4 cut(s) 44, 80, 175, 321
Hpy188I TCNGA 2 cut(s) 85, 118
Hpy188III TCNNGA 2 cut(s) 77, 346
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 3 cut(s) 80, 266, 350
HpyCH4III ACNGT 2 cut(s) 310, 436
HpyCH4V TGCA 4 cut(s) 140, 191, 250, 332
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 477
HpyF3I CTNAG 2 cut(s) 115, 171
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 3 cut(s) 370, 396, 427
LpnPI CCDG 8 cut(s) 32, 236, 258, 302, 353, 380, 429, 457
LweI GCATC 1 cut(s) 319
MaeI CTAG 4 cut(s) 77, 146, 197, 359
MaeIII GTNAC 1 cut(s) 268
MalI GATC 3 cut(s) 372, 398, 429
MboI GATC 3 cut(s) 370, 396, 427
MboII GAAGA 2 cut(s) 62, 65
MflI RGATCY 2 cut(s) 370, 427
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 127
MlyI GAGTC 2 cut(s) 74, 169
MmeI TCCRAC 1 cut(s) 455
MnlI CCTC 4 cut(s) 269, 342, 346, 401
Mox20I TGGCCA 1 cut(s) 127
MscI TGGCCA 1 cut(s) 127
Msp20I TGGCCA 1 cut(s) 127
MspR9I CCNGG 1 cut(s) 368
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 146, 477
NdeII GATC 3 cut(s) 370, 396, 427
NheI GCTAGC 1 cut(s) 145
NmuCI GTSAC 1 cut(s) 268
PcsI WCGNNNNNNNCGW 1 cut(s) 288
PfeI GAWTC 2 cut(s) 44, 321
PflMI CCANNNNNTGG 2 cut(s) 134, 471
PleI GAGTC 2 cut(s) 74, 169
PpsI GAGTC 2 cut(s) 74, 169
PpuMI RGGWCCY 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 253
Psp6I CCWGG 1 cut(s) 366
PspGI CCWGG 1 cut(s) 366
PspPI GGNCC 1 cut(s) 253
PspPPI RGGWCCY 1 cut(s) 253
PsuI RGATCY 2 cut(s) 370, 427
RsaI GTAC 1 cut(s) 312
RsaNI GTAC 1 cut(s) 311
Sau3AI GATC 3 cut(s) 370, 396, 427
Sau96I GGNCC 1 cut(s) 253
SchI GAGTC 2 cut(s) 74, 169
ScrFI CCNGG 1 cut(s) 368
SetI ASST 6 cut(s) 21, 147, 151, 258, 369, 453
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 253
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 1 cut(s) 5
SsiI CCGC 1 cut(s) 203
SspMI CTAG 4 cut(s) 77, 146, 197, 359
StyD4I CCNGG 1 cut(s) 366
TaaI ACNGT 2 cut(s) 310, 436
TaqI TCGA 1 cut(s) 219
TasI AATT 1 cut(s) 5
TatI WGTACW 1 cut(s) 310
TfiI GAWTC 2 cut(s) 44, 321
TscAI CASTG 1 cut(s) 313
TseFI GTSAC 1 cut(s) 268
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 17, 36, 300
TspRI CASTG 1 cut(s) 313
Van91I CCANNNNNTGG 2 cut(s) 134, 471
VpaK11BI GGWCC 1 cut(s) 253
XbaI TCTAGA 1 cut(s) 76
XspI CTAG 4 cut(s) 77, 146, 197, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.