AT4G08520

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002186
Physical Location & Seq
Forward (+)
5417209 .. 5420639
3431 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G08520.1

Sequence Viewer

Length: 546 bp
ATGGCAGGGACTAATGATTCTTGTCCTTTGGTAAAGAACATTCTTCTTCTAGACTCTGAAGGAAAGCGTGTGGCTGTCAAGTATTACTCCGATGATTGGGCAACTAATGCTTCCAAGTTAGCTTTTGAAAAATATGTCTTCTCGAAAACCTCCAAGACCAATGCTCGCACAGAAGCTGAGATCACACTGTTGGAGAGTAATATTGTTGTCTATAAGTTTGCCCAGGACCTGCATTTCTTTGTTACTGGAGGTGAAAATGAAAACGAGCTCGTCTTATCATCTGTTCTTCAAGGCTTTTTTGATGCTGTTGCGTTACTTCTGAGGAACAATGTTGAAAAGATGGAAGCCCTTGAAAACTTGGATCTCATCTTTTTGTGCCTTGATGAGATGGTTGATCAAGGGATGGTACTTGAAACAGACGCGAATGTTATTGCGGGGAAAGTAGCAATGCAAAGCGCAGAAGCAAGTGGTTCACTCTCTGAACAGACATTAACTCAAGCATTGGCAACAGCTCGGGAGCACCTTGCGAGAAGTCTTCTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Clat_adaptor_s

Protein Analysis

181

Amino Acids

19.88

Weight (kDa)

4.59

Isoelectric Point (pI)

34.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Clat_adaptor_s PF01217 11 - 151 6.3e-22 Clathrin adaptor complex small chain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 237
AccII CGCG 1 cut(s) 422
AciI CCGC 1 cut(s) 434
AclWI GGATC 1 cut(s) 369
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 1 cut(s) 408
AfiI CCNNNNNNNGG 1 cut(s) 96
AgsI TTSAA 5 cut(s) 128, 290, 335, 353, 413
AjnI CCWGG 1 cut(s) 222
AluBI AGCT 4 cut(s) 122, 176, 268, 512
AluI AGCT 4 cut(s) 122, 176, 268, 512
Alw21I GWGCWC 2 cut(s) 270, 522
AlwI GGATC 1 cut(s) 369
AlwNI CAGNNNCTG 2 cut(s) 176, 229
Ama87I CYCGRG 1 cut(s) 513
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 263
AvaI CYCGRG 1 cut(s) 513
AvaII GGWCC 1 cut(s) 226
BanII GRGCYC 1 cut(s) 270
BarI GAAGNNNNNNTAC 1 cut(s) 523
BbsI GAAGAC 2 cut(s) 130, 527
Bbv12I GWGCWC 2 cut(s) 270, 522
BccI CCATC 3 cut(s) 334, 382, 397
BciT130I CCWGG 1 cut(s) 224
BclI TGATCA 1 cut(s) 394
BfaI CTAG 1 cut(s) 50
BfuAI ACCTGC 1 cut(s) 237
Bme1390I CCNGG 1 cut(s) 224
Bme18I GGWCC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 513
BmgT120I GGNCC 1 cut(s) 226
BmrFI CCNGG 1 cut(s) 224
BmsI GCATC 1 cut(s) 292
BpiI GAAGAC 2 cut(s) 130, 527
BpmI CTGGAG 1 cut(s) 267
BpuEI CTTGAG 1 cut(s) 480
BsaJI CCNNGG 1 cut(s) 222
Bsc4I CCNNNNNNNGG 1 cut(s) 96
Bse1I ACTGG 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 1 cut(s) 224
BseDI CCNNGG 1 cut(s) 222
BseGI GGATG 1 cut(s) 408
BseLI CCNNNNNNNGG 1 cut(s) 96
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 2 cut(s) 168, 311
BseNI ACTGG 1 cut(s) 250
Bsh1236I CGCG 1 cut(s) 422
BsiHKAI GWGCWC 2 cut(s) 270, 522
BsiHKCI CYCGRG 1 cut(s) 513
BslFI GGGAC 1 cut(s) 22
BslI CCNNNNNNNGG 1 cut(s) 96
BsmFI GGGAC 1 cut(s) 22
BsoBI CYCGRG 1 cut(s) 513
Bsp1286I GDGCHC 2 cut(s) 270, 522
Bsp143I GATC 3 cut(s) 180, 361, 394
BspACI CCGC 1 cut(s) 434
BspCNI CTCAG 2 cut(s) 169, 312
BspFNI CGCG 1 cut(s) 422
BspMI ACCTGC 1 cut(s) 237
BspPI GGATC 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 3 cut(s) 180, 361, 394
Bst2UI CCWGG 1 cut(s) 224
Bst4CI ACNGT 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 107
BstC8I GCNNGC 1 cut(s) 166
BstDEI CTNAG 2 cut(s) 177, 320
BstF5I GGATG 1 cut(s) 408
BstFNI CGCG 1 cut(s) 422
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 3 cut(s) 183, 364, 397
BstMBI GATC 3 cut(s) 180, 361, 394
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNI CCWGG 1 cut(s) 224
BstSCI CCNGG 1 cut(s) 222
BstUI CGCG 1 cut(s) 422
BstV2I GAAGAC 2 cut(s) 130, 527
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BtsCI GGATG 1 cut(s) 408
BtsIMutI CAGTG 1 cut(s) 185
BveI ACCTGC 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 166
CaiI CAGNNNCTG 2 cut(s) 176, 229
CfoI GCGC 1 cut(s) 458
Cfr13I GGNCC 1 cut(s) 226
CseI GACGC 1 cut(s) 428
Csp6I GTAC 1 cut(s) 407
CviJI RGCY 7 cut(s) 74, 122, 176, 268, 294, 347, 512
CviKI_1 RGCY 7 cut(s) 74, 122, 176, 268, 294, 347, 512
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 2 cut(s) 177, 320
DpnI GATC 3 cut(s) 182, 363, 396
DpnII GATC 3 cut(s) 180, 361, 394
Ecl136II GAGCTC 1 cut(s) 268
Eco24I GRGCYC 1 cut(s) 270
Eco47I GGWCC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 268
Eco57I CTGAAG 1 cut(s) 78
Eco88I CYCGRG 1 cut(s) 513
EcoICRI GAGCTC 1 cut(s) 268
EcoO109I RGGNCCY 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 270
FaiI YATR 3 cut(s) 135, 213, 544
FaqI GGGAC 1 cut(s) 22
FauI CCCGC 1 cut(s) 427
FbaI TGATCA 1 cut(s) 394
FokI GGATG 1 cut(s) 415
FriOI GRGCYC 1 cut(s) 270
FspBI CTAG 1 cut(s) 50
GlaI GCGC 1 cut(s) 457
GsuI CTGGAG 1 cut(s) 267
HgaI GACGC 1 cut(s) 428
HhaI GCGC 1 cut(s) 458
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HinfI GANTC 2 cut(s) 17, 53
HphI GGTGA 1 cut(s) 263
Hpy166II GTNNAC 1 cut(s) 473
Hpy188I TCNGA 4 cut(s) 58, 91, 321, 481
Hpy188III TCNNGA 3 cut(s) 50, 142, 515
Hpy8I GTNNAC 1 cut(s) 473
HpyAV CCTTC 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 189
HpyCH4V TGCA 2 cut(s) 232, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 2 cut(s) 177, 320
HspAI GCGC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 394
Kzo9I GATC 3 cut(s) 180, 361, 394
LmnI GCTCC 1 cut(s) 517
LpnPI CCDG 4 cut(s) 209, 231, 236, 242
LweI GCATC 1 cut(s) 292
MaeI CTAG 1 cut(s) 50
MaeIII GTNAC 2 cut(s) 241, 312
MalI GATC 3 cut(s) 182, 363, 396
MboI GATC 3 cut(s) 180, 361, 394
MboII GAAGA 5 cut(s) 35, 38, 130, 278, 527
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 2 cut(s) 270, 522
MlyI GAGTC 1 cut(s) 47
MmeI TCCRAC 1 cut(s) 171
MnlI CCTC 3 cut(s) 160, 242, 315
MseI TTAA 1 cut(s) 491
MspR9I CCNGG 1 cut(s) 224
MvaI CCWGG 1 cut(s) 224
MvnI CGCG 1 cut(s) 422
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 3 cut(s) 180, 361, 394
PfeI GAWTC 1 cut(s) 17
PleI GAGTC 1 cut(s) 47
PpsI GAGTC 1 cut(s) 47
PpuMI RGGWCCY 1 cut(s) 226
Psp124BI GAGCTC 1 cut(s) 270
Psp5II RGGWCCY 1 cut(s) 226
Psp6I CCWGG 1 cut(s) 222
PspGI CCWGG 1 cut(s) 222
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
PstNI CAGNNNCTG 2 cut(s) 176, 229
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 491
Sau3AI GATC 3 cut(s) 180, 361, 394
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 47
ScrFI CCNGG 1 cut(s) 224
SduI GDGCHC 2 cut(s) 270, 522
SetI ASST 8 cut(s) 124, 152, 178, 231, 253, 270, 514, 525
SfaNI GCATC 1 cut(s) 292
SinI GGWCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 495
SmoI CTYRAG 1 cut(s) 495
SsiI CCGC 1 cut(s) 434
SspI AATATT 1 cut(s) 202
SspMI CTAG 1 cut(s) 50
SstI GAGCTC 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 143
TfiI GAWTC 1 cut(s) 17
Tru1I TTAA 1 cut(s) 491
Tru9I TTAA 1 cut(s) 491
TscAI CASTG 1 cut(s) 192
TspDTI ATGAA 1 cut(s) 273
TspRI CASTG 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 226
XbaI TCTAGA 1 cut(s) 49
XspI CTAG 1 cut(s) 50
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.