AT3G18590

early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001969
Physical Location & Seq
Forward (+)
6398632 .. 6399337
706 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G18590.1

Sequence Viewer

Length: 567 bp
ATGGATTCATCAAAGAAGATCATTATCGTTATGTTTCTTGTAACATTTTACATGTTTTCATGTGTTTCAAGCACTGAATTTGAAGTTGGAGGAGAAAATGGTTGGATCGTACCAAAATCTAAGACTCTTGGAGATGCGTTTAACCAATGGGCGTCAGATAACCGGTTTAAAGTTGGTGACACTCTCCGTTTCAAGTACACGAAAGACTCAGTGTTAGTGGTGTCAGAAGAAGAGTACAAGAAATGCAAAGCGACCAAACCACAACTCTACTCAAACAACGAAGACACTGTTTTCAAACTAGACCGACCCGGTTTGTTTTACTTCATCAGCGGTGTCTCTGGCCACTGCGAAAAGGGTCAGAAGATGATCGTAAAAGTTATGGAGACGGAGTCTTCTACAGAGTCTCCTCCTCCATCCTCTTCTTCTTCTTCTTCGTCATCATCATCTCTTCCGGCGTCAACTCCCAAGGCCAAGAAGAGCAATGCCTTCAAAACCGCAGTCCAGTTCAGTAGTTCCGGTTTTGTTGTCTCTGCTGTTCTGATTGTTTCAGTTTTTGGTTTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

188

Amino Acids

20.56

Weight (kDa)

8.88

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 45 - 120 2.6e-20 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 330, 495
AclWI GGATC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 340
AcsI RAATTY 1 cut(s) 77
AcyI GRCGYC 2 cut(s) 152, 455
AfaI GTAC 3 cut(s) 111, 197, 236
AflIII ACRYGT 1 cut(s) 51
AgeI ACCGGT 1 cut(s) 162
AgsI TTSAA 5 cut(s) 69, 83, 193, 295, 490
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
Alw26I GTCTC 4 cut(s) 340, 377, 408, 532
AlwI GGATC 1 cut(s) 113
AoxI GGCC 2 cut(s) 340, 468
ApoI RAATTY 1 cut(s) 77
ArsI GACNNNNNNTTYG 2 cut(s) 483, 515
AsiGI ACCGGT 1 cut(s) 162
AsuC2I CCSGG 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 188
BalI TGGCCA 1 cut(s) 342
BbsI GAAGAC 2 cut(s) 288, 384
BccI CCATC 1 cut(s) 421
BcnI CCSGG 1 cut(s) 309
BcoDI GTCTC 4 cut(s) 340, 377, 408, 532
BfaI CTAG 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 309
BmrFI CCNGG 1 cut(s) 309
BmsI GCATC 1 cut(s) 124
BpiI GAAGAC 2 cut(s) 288, 384
BpuMI CCSGG 1 cut(s) 309
BsaBI GATNNNNATC 1 cut(s) 23
BsaHI GRCGYC 2 cut(s) 152, 455
BsaJI CCNNGG 1 cut(s) 465
BsaWI WCCGGW 2 cut(s) 162, 515
BsaXI ACNNNNNCTCC 4 cut(s) 374, 388, 404, 418
Bse118I RCCGGY 1 cut(s) 162
Bse1I ACTGG 1 cut(s) 502
Bse3DI GCAATG 1 cut(s) 487
Bse8I GATNNNNATC 1 cut(s) 23
BseDI CCNNGG 1 cut(s) 465
BseGI GGATG 1 cut(s) 413
BseJI GATNNNNATC 1 cut(s) 23
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 502
BseRI GAGGAG 3 cut(s) 105, 396, 399
BshFI GGCC 2 cut(s) 342, 470
BshTI ACCGGT 1 cut(s) 162
BsiSI CCGG 4 cut(s) 163, 309, 452, 516
BsmAI GTCTC 4 cut(s) 340, 377, 408, 532
BsmBI CGTCTC 1 cut(s) 377
BsnI GGCC 2 cut(s) 342, 470
Bsp143I GATC 3 cut(s) 18, 105, 366
BspACI CCGC 2 cut(s) 330, 495
BspANI GGCC 2 cut(s) 342, 470
BspCNI CTCAG 1 cut(s) 221
BspPI GGATC 1 cut(s) 113
BspQI GCTCTTC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 162
BsrI ACTGG 1 cut(s) 502
BssAI RCCGGY 1 cut(s) 162
BssECI CCNNGG 1 cut(s) 465
BssMI GATC 3 cut(s) 18, 105, 366
BssNI GRCGYC 2 cut(s) 152, 455
BssT1I CCWWGG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 289
Bst6I CTCTTC 4 cut(s) 225, 424, 453, 470
BstACI GRCGYC 2 cut(s) 152, 455
BstDEI CTNAG 2 cut(s) 120, 208
BstF5I GGATG 1 cut(s) 413
BstKTI GATC 3 cut(s) 21, 108, 369
BstMAI GTCTC 4 cut(s) 340, 377, 408, 532
BstMBI GATC 3 cut(s) 18, 105, 366
BstNSI RCATGY 1 cut(s) 55
BstSCI CCNGG 1 cut(s) 307
BstSFI CTRYAG 1 cut(s) 396
BstV2I GAAGAC 2 cut(s) 288, 384
BsuRI GGCC 2 cut(s) 342, 470
BtsCI GGATG 1 cut(s) 413
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 4 cut(s) 72, 216, 285, 343
Cfr10I RCCGGY 1 cut(s) 162
CseI GACGC 2 cut(s) 141, 444
Csp6I GTAC 3 cut(s) 110, 196, 235
CspAI ACCGGT 1 cut(s) 162
CviAII CATG 2 cut(s) 52, 60
CviJI RGCY 2 cut(s) 342, 470
CviKI_1 RGCY 2 cut(s) 342, 470
CviQI GTAC 3 cut(s) 110, 196, 235
DdeI CTNAG 2 cut(s) 120, 208
DpnI GATC 3 cut(s) 20, 107, 368
DpnII GATC 3 cut(s) 18, 105, 366
DraI TTTAAA 1 cut(s) 169
EaeI YGGCCR 1 cut(s) 340
Eam1104I CTCTTC 4 cut(s) 225, 424, 453, 470
EarI CTCTTC 4 cut(s) 225, 424, 453, 470
Eco130I CCWWGG 1 cut(s) 465
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
Esp3I CGTCTC 1 cut(s) 377
FaeI CATG 2 cut(s) 55, 63
FaiI YATR 4 cut(s) 32, 53, 61, 380
FatI CATG 2 cut(s) 51, 59
FokI GGATG 1 cut(s) 400
FspBI CTAG 1 cut(s) 299
HaeIII GGCC 2 cut(s) 342, 470
HapII CCGG 4 cut(s) 163, 309, 452, 516
HgaI GACGC 2 cut(s) 141, 444
Hin1I GRCGYC 2 cut(s) 152, 455
Hin1II CATG 2 cut(s) 55, 63
HincII GTYRAC 1 cut(s) 459
HindII GTYRAC 1 cut(s) 459
HinfI GANTC 5 cut(s) 5, 124, 206, 389, 401
HpaII CCGG 4 cut(s) 163, 309, 452, 516
HphI GGTGA 1 cut(s) 188
Hpy166II GTNNAC 2 cut(s) 198, 459
Hpy188I TCNGA 4 cut(s) 157, 226, 360, 540
Hpy8I GTNNAC 2 cut(s) 198, 459
HpyAV CCTTC 1 cut(s) 496
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4V TGCA 1 cut(s) 246
HpyF3I CTNAG 2 cut(s) 120, 208
Hsp92I GRCGYC 2 cut(s) 152, 455
Hsp92II CATG 2 cut(s) 55, 63
Kzo9I GATC 3 cut(s) 18, 105, 366
LguI GCTCTTC 1 cut(s) 470
LpnPI CCDG 6 cut(s) 176, 322, 324, 465, 515, 529
LweI GCATC 1 cut(s) 124
MaeI CTAG 1 cut(s) 299
MaeIII GTNAC 2 cut(s) 40, 176
MalI GATC 3 cut(s) 20, 107, 368
MboI GATC 3 cut(s) 18, 105, 366
MlsI TGGCCA 1 cut(s) 342
MluCI AATT 1 cut(s) 77
MluNI TGGCCA 1 cut(s) 342
MlyI GAGTC 4 cut(s) 118, 200, 398, 410
MmeI TCCRAC 2 cut(s) 67, 83
MnlI CCTC 4 cut(s) 83, 417, 420, 427
Mox20I TGGCCA 1 cut(s) 342
MscI TGGCCA 1 cut(s) 342
MseI TTAA 2 cut(s) 141, 168
Msp20I TGGCCA 1 cut(s) 342
MspA1I CMGCKG 1 cut(s) 330
MspI CCGG 4 cut(s) 163, 309, 452, 516
MspR9I CCNGG 1 cut(s) 309
NciI CCSGG 1 cut(s) 309
NdeII GATC 3 cut(s) 18, 105, 366
NlaIII CATG 2 cut(s) 55, 63
NmuCI GTSAC 1 cut(s) 176
NspI RCATGY 1 cut(s) 55
PciI ACATGT 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 470
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 388
PinAI ACCGGT 1 cut(s) 162
PleI GAGTC 4 cut(s) 118, 200, 397, 409
PpsI GAGTC 4 cut(s) 118, 200, 397, 409
PscI ACATGT 1 cut(s) 51
PsyI GACNNNGTC 1 cut(s) 388
RsaI GTAC 3 cut(s) 111, 197, 236
RsaNI GTAC 3 cut(s) 110, 196, 235
SapI GCTCTTC 1 cut(s) 470
SaqAI TTAA 2 cut(s) 141, 168
Sau3AI GATC 3 cut(s) 18, 105, 366
SchI GAGTC 4 cut(s) 118, 200, 398, 410
ScrFI CCNGG 1 cut(s) 309
SfaNI GCATC 1 cut(s) 124
SfcI CTRYAG 1 cut(s) 396
Sse9I AATT 1 cut(s) 77
SsiI CCGC 2 cut(s) 330, 495
SspMI CTAG 1 cut(s) 299
StyD4I CCNGG 1 cut(s) 307
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 289
TaqII GACCGA 1 cut(s) 318
TasI AATT 1 cut(s) 77
TatI WGTACW 2 cut(s) 195, 234
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 141, 168
Tru9I TTAA 2 cut(s) 141, 168
TscAI CASTG 4 cut(s) 79, 216, 292, 350
TseFI GTSAC 1 cut(s) 176
Tsp45I GTSAC 1 cut(s) 176
TspDTI ATGAA 2 cut(s) 48, 313
TspGWI ACGGA 2 cut(s) 176, 401
TspRI CASTG 4 cut(s) 79, 216, 292, 350
Tth111I GACNNNGTC 1 cut(s) 388
XapI RAATTY 1 cut(s) 77
XceI RCATGY 1 cut(s) 55
XspI CTAG 1 cut(s) 299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.