RLG00000005366

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001969
Physical Location & Seq
Forward (+)
67124403 .. 67125059
657 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005366

Sequence Viewer

Length: 558 bp
ATGGAATCCTCCAAAGTATTTCTCTTTTTTCTTCTCTTTGCTATTCAGGTCGTCTTTGTAACATGTACTCAATTTCAAGTTGGTGACAAGAGCAATGGATGGGAGGTCCCCAAATCAAAGAGTGACCAAGATAGGTACAACCAATGGGCCTCTAAGAATAGGTTCAAAGTTGACGACACTCTCAATTTCAATTACAAGAAAAACTCAGACTCAGTTATGGTGGTGACGGAAGCTGAGTACGAAAAATGCCATTCCGATAATCCCATTTTCAGCTCTAACGATGGTGACTCGGTCTTCAAATTGGATCGACCCGGTTCGTTCTACTTCATTAGTGGGACTGCCGGACACTGCGAGAAAGGGCAGAAAATGATTGTCAAAGTCTTGGGATCACCGGCTGCTGGAACAGAAAGCCCACCACCTACTCAATCAGCAAACCAGAATTCAACTGAATCACCAGATCATCATGACATGAAGAACAATGCAGTTGCAATGCATGCTGTAGCTGCCATCTCTTTTACAACTTCTGTAATGTCCTTTCTTGGGGTCTTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

186

Amino Acids

20.57

Weight (kDa)

5.92

Isoelectric Point (pI)

32.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 39 - 121 1.1e-23 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 312, 394
AcsI RAATTY 1 cut(s) 439
AfaI GTAC 3 cut(s) 67, 137, 239
AfiI CCNNNNNNNGG 2 cut(s) 398, 540
AflIII ACRYGT 1 cut(s) 62
AgsI TTSAA 5 cut(s) 77, 166, 190, 298, 444
AluBI AGCT 3 cut(s) 233, 273, 503
AluI AGCT 3 cut(s) 233, 273, 503
AlwI GGATC 2 cut(s) 312, 394
AoxI GGCC 1 cut(s) 147
ApeKI GCWGC 2 cut(s) 395, 503
ApoI RAATTY 1 cut(s) 439
Asp700I GAANNNNTTC 1 cut(s) 161
AspS9I GGNCC 2 cut(s) 106, 147
AsuC2I CCSGG 1 cut(s) 312
AsuHPI GGTGA 5 cut(s) 95, 235, 296, 381, 444
AvaII GGWCC 1 cut(s) 106
BbsI GAAGAC 1 cut(s) 286
BbvI GCAGC 2 cut(s) 382, 490
BccI CCATC 3 cut(s) 93, 275, 515
BcnI CCSGG 1 cut(s) 312
BfaI CTAG 1 cut(s) 556
BfmI CTRYAG 1 cut(s) 498
BisI GCNGC 2 cut(s) 396, 504
BlsI GCNGC 2 cut(s) 397, 505
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 2 cut(s) 106, 147
BmiI GGNNCC 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 312
BpiI GAAGAC 1 cut(s) 286
BpuMI CCSGG 1 cut(s) 312
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 540
Bse118I RCCGGY 1 cut(s) 391
Bse3DI GCAATG 2 cut(s) 100, 495
BseGI GGATG 1 cut(s) 104
BseLI CCNNNNNNNGG 2 cut(s) 398, 540
BseMI GCAATG 2 cut(s) 100, 495
BseMII CTCAG 3 cut(s) 219, 225, 225
BseXI GCAGC 2 cut(s) 382, 490
BshFI GGCC 1 cut(s) 149
BsiSI CCGG 3 cut(s) 312, 342, 392
BslFI GGGAC 2 cut(s) 92, 349
BslI CCNNNNNNNGG 2 cut(s) 398, 540
BsmFI GGGAC 2 cut(s) 92, 349
BsnI GGCC 1 cut(s) 149
Bsp143I GATC 3 cut(s) 304, 386, 457
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 3 cut(s) 218, 224, 226
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 1 cut(s) 108
BspPI GGATC 2 cut(s) 312, 394
BsrDI GCAATG 2 cut(s) 100, 495
BsrFI RCCGGY 1 cut(s) 391
BssAI RCCGGY 1 cut(s) 391
BssMI GATC 3 cut(s) 304, 386, 457
BstAPI GCANNNNNTGC 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 495
BstDEI CTNAG 4 cut(s) 153, 205, 211, 234
BstF5I GGATG 1 cut(s) 104
BstKTI GATC 3 cut(s) 307, 389, 460
BstMBI GATC 3 cut(s) 304, 386, 457
BstMWI GCNNNNNNNGC 2 cut(s) 494, 503
BstNSI RCATGY 2 cut(s) 66, 497
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 498
BstV1I GCAGC 2 cut(s) 382, 490
BstV2I GAAGAC 1 cut(s) 286
BsuRI GGCC 1 cut(s) 149
BtsCI GGATG 1 cut(s) 104
BtsI GCAGTG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 346
Cac8I GCNNGC 1 cut(s) 495
CciI TCATGA 1 cut(s) 463
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 106, 147
Csp6I GTAC 3 cut(s) 66, 136, 238
CviAII CATG 4 cut(s) 63, 464, 469, 494
CviJI RGCY 6 cut(s) 149, 233, 273, 395, 411, 503
CviKI_1 RGCY 6 cut(s) 149, 233, 273, 395, 411, 503
CviQI GTAC 3 cut(s) 66, 136, 238
DdeI CTNAG 4 cut(s) 153, 205, 211, 234
DpnI GATC 3 cut(s) 306, 388, 459
DpnII GATC 3 cut(s) 304, 386, 457
Eco47I GGWCC 1 cut(s) 106
EcoO109I RGGNCCY 1 cut(s) 106
EcoRI GAATTC 1 cut(s) 439
EcoT22I ATGCAT 1 cut(s) 495
FaeI CATG 4 cut(s) 66, 467, 472, 497
FaiI YATR 5 cut(s) 64, 218, 465, 470, 495
FaqI GGGAC 2 cut(s) 92, 349
FatI CATG 4 cut(s) 62, 463, 468, 493
Fnu4HI GCNGC 2 cut(s) 396, 504
FokI GGATG 1 cut(s) 111
Fsp4HI GCNGC 2 cut(s) 396, 504
FspBI CTAG 1 cut(s) 556
GluI GCNGC 2 cut(s) 396, 504
HaeIII GGCC 1 cut(s) 149
HapII CCGG 3 cut(s) 312, 342, 392
Hin1II CATG 4 cut(s) 66, 467, 472, 497
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 4 cut(s) 5, 209, 287, 449
HpaII CCGG 3 cut(s) 312, 342, 392
HphI GGTGA 5 cut(s) 95, 235, 296, 381, 444
Hpy166II GTNNAC 1 cut(s) 172
Hpy188I TCNGA 2 cut(s) 208, 256
Hpy188III TCNNGA 1 cut(s) 464
Hpy8I GTNNAC 1 cut(s) 172
HpyCH4V TGCA 3 cut(s) 482, 488, 493
HpyF10VI GCNNNNNNNGC 2 cut(s) 494, 503
HpyF3I CTNAG 4 cut(s) 153, 205, 211, 234
Hsp92II CATG 4 cut(s) 66, 467, 472, 497
Kzo9I GATC 3 cut(s) 304, 386, 457
LpnPI CCDG 7 cut(s) 32, 325, 355, 384, 405, 449, 468
Lsp1109I GCAGC 2 cut(s) 382, 490
MaeI CTAG 1 cut(s) 556
MaeIII GTNAC 5 cut(s) 58, 83, 122, 223, 284
MalI GATC 3 cut(s) 306, 388, 459
MboI GATC 3 cut(s) 304, 386, 457
MboII GAAGA 4 cut(s) 23, 286, 484, 544
MluCI AATT 5 cut(s) 71, 184, 190, 299, 439
MlyI GAGTC 2 cut(s) 203, 281
MnlI CCTC 3 cut(s) 19, 97, 160
Mph1103I ATGCAT 1 cut(s) 495
MroXI GAANNNNTTC 1 cut(s) 161
MspI CCGG 3 cut(s) 312, 342, 392
MspR9I CCNGG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 494, 503
NciI CCSGG 1 cut(s) 312
NdeII GATC 3 cut(s) 304, 386, 457
NlaIII CATG 4 cut(s) 66, 467, 472, 497
NlaIV GGNNCC 1 cut(s) 108
NmuCI GTSAC 4 cut(s) 83, 122, 223, 284
NsiI ATGCAT 1 cut(s) 495
NspI RCATGY 2 cut(s) 66, 497
PaeI GCATGC 1 cut(s) 497
PagI TCATGA 1 cut(s) 463
PciI ACATGT 1 cut(s) 62
PdmI GAANNNNTTC 1 cut(s) 161
PfeI GAWTC 2 cut(s) 5, 449
PflFI GACNNNGTC 1 cut(s) 290
PkrI GCNGC 2 cut(s) 397, 505
PleI GAGTC 2 cut(s) 203, 281
PpsI GAGTC 2 cut(s) 203, 281
PpuMI RGGWCCY 1 cut(s) 106
PscI ACATGT 1 cut(s) 62
Psp5II RGGWCCY 1 cut(s) 106
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 2 cut(s) 106, 147
PspPPI RGGWCCY 1 cut(s) 106
PsyI GACNNNGTC 1 cut(s) 290
RsaI GTAC 3 cut(s) 67, 137, 239
RsaNI GTAC 3 cut(s) 66, 136, 238
SatI GCNGC 2 cut(s) 396, 504
Sau3AI GATC 3 cut(s) 304, 386, 457
Sau96I GGNCC 2 cut(s) 106, 147
SchI GAGTC 2 cut(s) 203, 281
ScrFI CCNGG 1 cut(s) 312
SetI ASST 8 cut(s) 51, 108, 137, 164, 235, 275, 421, 505
SfcI CTRYAG 1 cut(s) 498
SinI GGWCC 1 cut(s) 106
SphI GCATGC 1 cut(s) 497
Sse9I AATT 5 cut(s) 71, 184, 190, 299, 439
SspMI CTAG 1 cut(s) 556
StyD4I CCNGG 1 cut(s) 310
TaqI TCGA 1 cut(s) 307
TaqII GACCGA 1 cut(s) 280
TasI AATT 5 cut(s) 71, 184, 190, 299, 439
TatI WGTACW 1 cut(s) 65
TfiI GAWTC 2 cut(s) 5, 449
TscAI CASTG 1 cut(s) 353
TseFI GTSAC 4 cut(s) 83, 122, 223, 284
TseI GCWGC 2 cut(s) 395, 503
Tsp45I GTSAC 4 cut(s) 83, 122, 223, 284
TspDTI ATGAA 2 cut(s) 316, 485
TspGWI ACGGA 1 cut(s) 242
TspRI CASTG 1 cut(s) 353
Tth111I GACNNNGTC 1 cut(s) 290
VpaK11BI GGWCC 1 cut(s) 106
XapI RAATTY 1 cut(s) 439
XceI RCATGY 2 cut(s) 66, 497
XmnI GAANNNNTTC 1 cut(s) 161
XspI CTAG 1 cut(s) 556
Zsp2I ATGCAT 1 cut(s) 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.