Rw6G026630

Early nodulin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001969
Physical Location & Seq
Reverse (-)
50059689 .. 50061886
2198 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026630.1

Sequence Viewer

Length: 522 bp
ATGAAACAATTTGATTCTTTCTTACTTGAAGTAATACCACAACCATTTTTTATGTTTAATCTTTTGTTATTATTTAAGGCCCTGATATTTTATGATTTCGTCTTTCGATCAGGAGGTCCAAATTCTGGTTTTCAGAGATTGAAACTTGATCATATTCTTGGCAATTTGGATCCTTTGTATATAAATGTTCAGAAAGCTGAGTACGAGAAATGCCATTCTGATAATCCCATTTTCAGCTCTAACGATGGTGACTCGGTTTTCAAATTGGATCGACCTGGTTCGTTCTACTTCATTAGAGGGACTGCCGGACACTGCGAGAAAGGGCAGAAAATGATTGTCAAAGTCTTGGGATCACCGGCTGCTGGAACAGAAAGCCCACCACCTGCTCAATCAGCAAACCAGAATGCAACTGAATCACCAGATCATCATGACATGAAGAACAATGCAGTTGCAATGCATGCTGTAGCTGCCATCTCTTTTACAAGTTCCGTAATATCATTTCTTGGGGTCTTTTTCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cu_bind_like

Protein Analysis

173

Amino Acids

19.2

Weight (kDa)

6.28

Isoelectric Point (pI)

49.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu_bind_like PF02298 62 - 109 2.9e-09 Plastocyanin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 391
Acc36I ACCTGC 1 cut(s) 391
AccB7I CCANNNNNTGG 1 cut(s) 125
AclWI GGATC 4 cut(s) 164, 177, 276, 358
AcsI RAATTY 1 cut(s) 121
AfaI GTAC 1 cut(s) 203
AfiI CCNNNNNNNGG 2 cut(s) 125, 362
AgsI TTSAA 3 cut(s) 29, 142, 262
AjnI CCWGG 1 cut(s) 274
AluBI AGCT 3 cut(s) 197, 237, 467
AluI AGCT 3 cut(s) 197, 237, 467
AlwI GGATC 4 cut(s) 164, 177, 276, 358
AoxI GGCC 1 cut(s) 78
ApeKI GCWGC 2 cut(s) 359, 467
ApoI RAATTY 1 cut(s) 121
AspS9I GGNCC 2 cut(s) 79, 116
AsuHPI GGTGA 3 cut(s) 260, 345, 408
AvaII GGWCC 1 cut(s) 116
BamHI GGATCC 1 cut(s) 169
BbvI GCAGC 2 cut(s) 346, 454
BccI CCATC 2 cut(s) 239, 479
BciT130I CCWGG 1 cut(s) 276
BclI TGATCA 1 cut(s) 148
BfaI CTAG 1 cut(s) 520
BfmI CTRYAG 1 cut(s) 462
BfuAI ACCTGC 1 cut(s) 391
BisI GCNGC 2 cut(s) 360, 468
BlsI GCNGC 2 cut(s) 361, 469
Bme1390I CCNGG 1 cut(s) 276
Bme18I GGWCC 1 cut(s) 116
BmgT120I GGNCC 2 cut(s) 79, 116
BmiI GGNNCC 1 cut(s) 171
BmrFI CCNGG 1 cut(s) 276
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 362
Bse118I RCCGGY 1 cut(s) 355
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 276
BseLI CCNNNNNNNGG 2 cut(s) 125, 362
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 189
BseXI GCAGC 2 cut(s) 346, 454
BshFI GGCC 1 cut(s) 80
BsiSI CCGG 2 cut(s) 306, 356
BslFI GGGAC 1 cut(s) 313
BslI CCNNNNNNNGG 2 cut(s) 125, 362
BsmFI GGGAC 1 cut(s) 313
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 80
Bsp143I GATC 6 cut(s) 107, 148, 169, 268, 350, 421
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 1 cut(s) 190
BspHI TCATGA 1 cut(s) 427
BspLI GGNNCC 1 cut(s) 171
BspMI ACCTGC 1 cut(s) 391
BspPI GGATC 4 cut(s) 164, 177, 276, 358
BsrDI GCAATG 1 cut(s) 459
BsrFI RCCGGY 1 cut(s) 355
BssAI RCCGGY 1 cut(s) 355
BssMI GATC 6 cut(s) 107, 148, 169, 268, 350, 421
Bst2UI CCWGG 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 458
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 1 cut(s) 198
BstKTI GATC 6 cut(s) 110, 151, 172, 271, 353, 424
BstMBI GATC 6 cut(s) 107, 148, 169, 268, 350, 421
BstMWI GCNNNNNNNGC 3 cut(s) 392, 458, 467
BstNI CCWGG 1 cut(s) 276
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 1 cut(s) 274
BstSFI CTRYAG 1 cut(s) 462
BstV1I GCAGC 2 cut(s) 346, 454
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BsuRI GGCC 1 cut(s) 80
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
BveI ACCTGC 1 cut(s) 391
Cac8I GCNNGC 1 cut(s) 459
CciI TCATGA 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 2 cut(s) 79, 116
CsiI ACCWGGT 1 cut(s) 274
Csp6I GTAC 1 cut(s) 202
CviAII CATG 3 cut(s) 428, 433, 458
CviJI RGCY 6 cut(s) 80, 197, 237, 359, 375, 467
CviKI_1 RGCY 6 cut(s) 80, 197, 237, 359, 375, 467
CviQI GTAC 1 cut(s) 202
DdeI CTNAG 1 cut(s) 198
DpnI GATC 6 cut(s) 109, 150, 171, 270, 352, 423
DpnII GATC 6 cut(s) 107, 148, 169, 268, 350, 421
Eco47I GGWCC 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 79
EcoRII CCWGG 1 cut(s) 274
EcoT22I ATGCAT 1 cut(s) 459
FaeI CATG 3 cut(s) 431, 436, 461
FaiI YATR 8 cut(s) 53, 93, 153, 180, 182, 429, 434, 459
FaqI GGGAC 1 cut(s) 313
FatI CATG 3 cut(s) 427, 432, 457
FbaI TGATCA 1 cut(s) 148
Fnu4HI GCNGC 2 cut(s) 360, 468
Fsp4HI GCNGC 2 cut(s) 360, 468
FspBI CTAG 1 cut(s) 520
GluI GCNGC 2 cut(s) 360, 468
HaeIII GGCC 1 cut(s) 80
HapII CCGG 2 cut(s) 306, 356
Hin1II CATG 3 cut(s) 431, 436, 461
HinfI GANTC 3 cut(s) 14, 251, 413
HpaII CCGG 2 cut(s) 306, 356
HphI GGTGA 3 cut(s) 260, 345, 408
Hpy188I TCNGA 3 cut(s) 135, 192, 220
Hpy188III TCNNGA 2 cut(s) 111, 428
HpyCH4V TGCA 4 cut(s) 407, 446, 452, 457
HpyF10VI GCNNNNNNNGC 3 cut(s) 392, 458, 467
HpyF3I CTNAG 1 cut(s) 198
Hsp92II CATG 3 cut(s) 431, 436, 461
Ksp22I TGATCA 1 cut(s) 148
Kzo9I GATC 6 cut(s) 107, 148, 169, 268, 350, 421
Lsp1109I GCAGC 2 cut(s) 346, 454
MabI ACCWGGT 1 cut(s) 274
MaeI CTAG 1 cut(s) 520
MaeIII GTNAC 1 cut(s) 248
MalI GATC 6 cut(s) 109, 150, 171, 270, 352, 423
MboI GATC 6 cut(s) 107, 148, 169, 268, 350, 421
MboII GAAGA 2 cut(s) 448, 508
MflI RGATCY 1 cut(s) 169
MluCI AATT 4 cut(s) 8, 121, 163, 263
MlyI GAGTC 1 cut(s) 245
MnlI CCTC 2 cut(s) 107, 290
Mph1103I ATGCAT 1 cut(s) 459
MseI TTAA 2 cut(s) 57, 75
MspI CCGG 2 cut(s) 306, 356
MspR9I CCNGG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 409
MvaI CCWGG 1 cut(s) 276
MwoI GCNNNNNNNGC 3 cut(s) 392, 458, 467
NdeII GATC 6 cut(s) 107, 148, 169, 268, 350, 421
NlaIII CATG 3 cut(s) 431, 436, 461
NlaIV GGNNCC 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 248
NsiI ATGCAT 1 cut(s) 459
NspI RCATGY 1 cut(s) 461
PaeI GCATGC 1 cut(s) 461
PagI TCATGA 1 cut(s) 427
PaqCI CACCTGC 1 cut(s) 391
PctI GAATGC 1 cut(s) 409
PfeI GAWTC 2 cut(s) 14, 413
PflMI CCANNNNNTGG 1 cut(s) 125
PkrI GCNGC 2 cut(s) 361, 469
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 274
PspGI CCWGG 1 cut(s) 274
PspN4I GGNNCC 1 cut(s) 171
PspPI GGNCC 2 cut(s) 79, 116
PsuI RGATCY 1 cut(s) 169
RsaI GTAC 1 cut(s) 203
RsaNI GTAC 1 cut(s) 202
SaqAI TTAA 2 cut(s) 57, 75
SatI GCNGC 2 cut(s) 360, 468
Sau3AI GATC 6 cut(s) 107, 148, 169, 268, 350, 421
Sau96I GGNCC 2 cut(s) 79, 116
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 1 cut(s) 276
SetI ASST 6 cut(s) 118, 199, 239, 277, 385, 469
SexAI ACCWGGT 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 462
SinI GGWCC 1 cut(s) 116
SphI GCATGC 1 cut(s) 461
Sse9I AATT 4 cut(s) 8, 121, 163, 263
SspMI CTAG 1 cut(s) 520
StyD4I CCNGG 1 cut(s) 274
TaqI TCGA 2 cut(s) 106, 271
TasI AATT 4 cut(s) 8, 121, 163, 263
TfiI GAWTC 2 cut(s) 14, 413
Tru1I TTAA 2 cut(s) 57, 75
Tru9I TTAA 2 cut(s) 57, 75
TscAI CASTG 1 cut(s) 317
TseFI GTSAC 1 cut(s) 248
TseI GCWGC 2 cut(s) 359, 467
Tsp45I GTSAC 1 cut(s) 248
TspDTI ATGAA 3 cut(s) 17, 280, 449
TspGWI ACGGA 1 cut(s) 478
TspRI CASTG 1 cut(s) 317
Van91I CCANNNNNTGG 1 cut(s) 125
VpaK11BI GGWCC 1 cut(s) 116
XapI RAATTY 1 cut(s) 121
XceI RCATGY 1 cut(s) 461
XspI CTAG 1 cut(s) 520
Zsp2I ATGCAT 1 cut(s) 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.